Article
Immunology
Ping Li, Zengli Guo, Yisong Y. Wan
Summary: Research has shown that SKI expression plays a critical role in the differentiation of pathogenic Th17 cells, and controlling SKI expression can modulate the function of these cells and treat Th17-related diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Ulas Ceylan, Steffen Haupeltshofer, Laura Kaemper, Justus Dann, Bjorn Ambrosius, Ralf Gold, Simon Faissner
Summary: Clozapine regulates microglial function and attenuates chronic EAE, even in a therapeutic treatment paradigm. This well-defined generic medication might be considered as a promising add-on therapeutic for further development in progressive MS.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nozomi Sachi, Naganori Kamiyama, Benjawan Saechue, Sotaro Ozaka, Astri Dewayani, Shimpei Ariki, Thanyakorn Chalalai, Yasuhiro Soga, Chiaki Fukuda, Yomei Kagoshima, Supanuch Ekronarongchai, Takashi Kobayashi
Summary: Multiple sclerosis is an autoimmune disease characterized by immune attack on the nerve myelin sheath. The balance between pathogenic Th17 cells and regulatory Treg cells, expressing chemokine receptor CCR6, is crucial in determining disease activity. The role of CCL20, the ligand of CCR6, in attracting immune cells to the central nervous system (CNS) in multiple sclerosis has not been fully understood. This study generated CCL20-knockout and CCR6-knockout mice using CRISPR/Cas9 system and found that the migratory function mediated by CCL20/CCR6 may not be necessary for the onset of experimental autoimmune encephalomyelitis (EAE).
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Andrea Herrero-Cervera, Carla Espinos-Estevez, Susana Martin-Vano, Alida Taberner-Cortes, Maria Aguilar-Ballester, Angela Vinue, Laura Piqueras, Sergio Martinez-Hervas, Herminia Gonzalez-Navarro
Summary: The study suggests a protective role of LIGHT in preventing VSMC trans-differentiation and maintaining their contractile characteristics. Loss of LIGHT exacerbates AAA severity and affects the gene expression of VSMCs.
Article
Immunology
Kota Moriguchi, Katsuichi Miyamoto, Yuta Fukumoto, Susumu Kusunoki
Summary: The study found that changing the number of daylight hours affects the course of experimental autoimmune encephalomyelitis (EAE) disease, with a 24-hour dark or light cycle environment upregulating internal corticosterone secretion and ameliorating the disease process.
JOURNAL OF NEUROIMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Angela A. Wang, Jennifer L. Gommerman, Olga L. Rojas
Summary: B cells play a critical role in the adaptive immune system by differentiating into plasmablasts and plasma cells to produce antibodies. Plasmablasts/plasma cells can survive for years in the bone marrow and intestine, producing cytokines and acting as regulatory cells.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Katharina Robichon, Sven Sondhauss, T. William Jordan, Robert A. Keyzers, Bronwen Connor, Anne C. La Flamme
Summary: This study investigated the tissue distribution and localization of clozapine in the brain using mass spectrometry techniques and found that clozapine was enriched in specific brain regions and significantly elevated in serum during EAE. Additionally, changes in dopamine receptor expression on brain-resident and infiltrating immune cells during EAE were observed, providing insights into how neuroinflammation alters the distribution and downstream effects of clozapine.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Liubov Beduleva, Tatyana Khramova, Alexandr Sidorov, Alexey Terentiev, Nadezhda Abisheva, Daria Menshikova, Pavel Ivanov, Kseniya Fomina, Anna Gorbushina, Natalia Shklyaeva, Igor Menshikov
Summary: The study found that stimulation of regRF production through IgG Fc fragments bearing regRF epitopes can alleviate symptoms of experimental autoimmune encephalomyelitis in rats. Conversely, immunization with Fc fragments without these epitopes worsened the condition.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Immunology
Yingying Wei, Zachary Braunstein, Jun Chen, Xinwen Min, Handong Yang, Lihua Duan, Lingli Dong, Jixin Zhong
Summary: Multiple sclerosis (MS) is an autoimmune disease mediated by T cells, but the exact signaling pathways involved in effector T cells in MS are not well understood. This study investigated the role of Janus kinase 2 (JAK2) and its potential as a therapeutic target in MS. The results showed that knockout of JAK2 in T cells prevented the development of MS in mice. In vitro experiments also demonstrated that JAK2 disruption suppressed the differentiation of TH1 cells and the production of IFN gamma. These findings suggest that overactive JAK2 signaling in T lymphocytes plays a critical role in MS and could be targeted for therapeutic intervention.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chao Zheng, Lingling Liu, Caiyun Liu, Fengna Chu, Yue Lang, Shan Liu, Yan Mi, Jie Zhu, Tao Jin
Summary: Inducing tolerogenic dendritic cells (tDCs) with low RelB expression could effectively alleviate symptoms and reduce immune cell infiltration and demyelination in experimental autoimmune encephalomyelitis (EAE) mouse model.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Chiara Camponeschi, Maria De Carluccio, Susanna Amadio, Maria Elisabetta Clementi, Beatrice Sampaolese, Cinzia Volonte, Maria Tredicine, Vincenzo Romano Spica, Rosa Di Liddo, Francesco Ria, Fabrizio Michetti, Gabriele Di Sante
Summary: The study demonstrated a direct correlation between S100B and inflammatory processes, and investigated the effect of AA on a mouse model of multiple sclerosis by inhibiting astrocytic S100B synthesis. The results showed that the AA-treated group had milder symptoms in the early phase of the disease and reduced various pathological features.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Anwar Fathollahi, Seyed Mahmoud Hashemi, Mostafa Haji Molla Hoseini, Sajjad Tavakoli, Elnaz Farahani, Farshid Yeganeh
Summary: Treatment with intranasal administration of MSC-SEV and MSC showed a significant decrease in clinical scores in EAE mice, with MSC-SEV being more effective than MSC. The therapy increased immunomodulatory responses, including an increase in the frequency of Foxp3+ CD25+ regulatory T cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Immunology
Byunghyun Park, Yu Sun Jeong, Wonseok Hu, Mingyu Lee, Ji Cheol Kim, Geon Ho Bae, Yong-Soo Bae, Yoe-Sik Bae
Summary: Multiple sclerosis (MS) is a disease that damages the brain and spinal cord, leading to paralysis. Recent research has shown that B cells play a significant role in the pathogenesis of MS, as autoantibodies from B cells are closely related to central nervous system damage and prognosis. Regulating the activity of antibody secreting cells may be linked to the severity of MS symptoms.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Shishi Shen, Xi Cheng, Luyao Zhou, Yipeng Zhao, Hai Wang, Jie Zhang, Xiaobo Sun, Yuge Wang, Yaqing Shu, Yanteng Xu, Yu Tao, Mingqiang Li, Zhengqi Lu, Wei Cai, Guangjun Nie, Wei Qiu
Summary: This study developed neutrophil-derived nanovesicles (NNVs) to enhance the efficiency of myelin debris clearance in microglia for multiple sclerosis (MS) therapy. The results showed that NNVs treatment ameliorated lesional neuroinflammation and improved neurological functions and white matter integrity in a mouse model of MS. Additionally, NNVs treatment upregulated the expression of NRF2 in microglia and activated the transcription of RUBCN, leading to enhanced phagocytosis of myelin debris.
Article
Immunology
Wenlin Hao, Qinghua Luo, Michael D. Menger, Klaus Fassbender, Yang Liu
Summary: Anti-CD52 therapy demonstrates neuroprotective effects in EAE mice by reducing T lymphocyte counts, preventing myelin loss, and protecting neurons. Neuronal BDNF regulates the neuroprotective and anti-inflammatory effects of CD52 antibody in EAE mice.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Franziska K. Merkt, Flaminia Mazzone, Shabnam Shaneh Sazzadeh, Lorand Bonda, Larissa K. E. Hinz, Irina Gruber, Karin Buchholz, Christoph Janiak, Klaus Pfeffer, Thomas J. J. Mueller
Summary: A gold-catalyzed cycloisomerization reaction was used to synthesize a series of luminescent silylated heterocycles, whose optical properties can be tuned by different functional groups. These compounds also showed promising anti-parasitic activities, making them potential candidates for novel anti-parasitic therapies.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Immunology
Anne Tersteegen, Ursula R. Sorg, Richard Virgen-Slane, Marcel Helle, Patrick Petzsch, Ildiko R. Dunay, Karl Kohrer, Daniel Degrandi, Carl F. Ware, Klaus Pfeffer
Summary: LT beta receptor plays a critical role in regulating adaptive immune responses to control T. gondii infection. LT beta receptor deficiency leads to globally altered IFN-gamma regulation, absence of parasite-specific IgG response, and severe loss of immune control over the parasites.
INFECTION AND IMMUNITY
(2021)
Review
Cell Biology
Rafael Leite Dantas, Jana Freff, Oliver Ambree, Eva C. Beins, Andreas J. Forstner, Udo Dannlowski, Bernhard T. Baune, Stefanie Scheu, Judith Alferink
Summary: Dendritic cells (DCs), a heterogeneous population of myeloid cells, are believed to play a role in peripheral immune responses and neuroinflammation in mood disorders, although their specific mechanisms of involvement remain unclear. The review summarizes current knowledge on the role of DCs in mood disorders and discusses their impact on neuroinflammation and behavior in animal models of AD.
Article
Biochemistry & Molecular Biology
Ursula R. Sorg, Nicole Kuepper, Julia Mock, Anne Tersteegen, Patrick Petzsch, Karl Koehrer, Thomas Hehlgans, Klaus Pfeffer
Summary: LTβR is crucial for efficient liver regeneration in hepatocytes, while the cooperating role of LTβR and TNFRp55 signaling pathways helps upregulate a set of target genes critical for liver regeneration.
BIOLOGICAL CHEMISTRY
(2021)
Article
Infectious Diseases
Bayaraa Baljin, Ulziijargal Gurjav, Khosbayar Tulgaa, Ganbaatar Baldan, Batbaatar Gunchin, Tsogtsaikhan Sandag, Klaus Pfeffer, Andreas F. Wendel, Colin R. MacKenzie
Summary: The study found that the colonization rate of gram-negative multi-drug resistant organisms was high at admission and doubled during hospitalization in Mongolia's National Trauma Center. Enterobacterales was the predominant colonizer and highly resistant to 3rd generation cephalosporins, indicating the need for improved infection control policies and antibiotic stewardship programs.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Biotechnology & Applied Microbiology
Tobias Beer, Sebastian Haensch, Klaus Pfeffer, Sander H. J. Smits, Stefanie Weidtkamp-Peters, Lutz Schmitt
Summary: Secretion systems play a vital role in Gram-negative bacteria by allowing communication with the outside world. This study focused on the hemolysin A (HlyA) type I secretion system (T1SS) in Escherichia coli and analyzed its localization and clustering. The results showed that the amount of ToIC, a component of the T1SS, was not affected by overexpression of the HlyBD complex. Superresolution microscopy revealed that the HlyA T1SS did not exhibit polarization and the size of T1SS clusters did not correlate with outer membrane islands.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Immunology
Marc Dalod, Stefanie Scheu
Summary: DCs play a crucial role in antigen presentation and orchestrate information transfer in the immune system. Mouse models provide valuable insights into the different functions of DC types and their activation states. This article provides comprehensive information on selecting mutant mouse strains for DC research and discusses the advantages, limitations, and future perspectives of these models.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Oncology
Jessica M. Salmon, Izabela Todorovski, Kym L. Stanley, Claudia Bruedigam, Conor J. Kearney, Luciano G. Martelotto, Fernando Rossello, Timothy Semple, Gisela Mir Arnau, Magnus Zethoven, Michael Bots, Stefan Bjelosevic, Leonie A. Cluse, Peter J. Fraser, Veronique Litalien, Eva Vidacs, Kate McArthur, Antony Y. Matthews, Elise Gressier, Nicole A. de Weerd, Jens Lichte, Madison J. Kelly, Simon J. Hogg, Paul J. Hertzog, Lev M. Kats, Stephin J. Vervoort, Daniel D. De Carvalho, Stefanie Scheu, Sammy Bedoui, Benjamin T. Kile, Steven W. Lane, Andrew C. Perkins, Andrew H. Wei, Pilar M. Dominguez, Ricky W. Johnstone
Summary: Inhibition of HDACs can induce differentiation and therapeutic effects in AML cells, and epigenetic rewiring of pDCs enhances antitumor immunity, offering a new therapeutic approach.
Article
Immunology
Xiaoli Yang, Shafaqat Ali, Manman Zhao, Lisa Richter, Vanessa Schaefer, Julian Schliehe-Diecks, Marian Frank, Jing Qi, Pia-Katharina Larsen, Jennifer Skerra, Heba Islam, Thorsten Wachtmeister, Christina Alter, Anfei Huang, Sanil Bhatia, Karl Koehrer, Carsten Kirschning, Heike Weighardt, Ulrich Kalinke, Rainer Kalscheuer, Markus Uhrberg, Stefanie Scheu
Summary: This study reveals that BEA activates BMDCs and induces the production of inflammatory cytokines and chemokines, which is dependent on the MyD88 and TRIF signaling pathways as well as TLR4. These findings contribute to a better understanding of the mechanism of action of BEA in immune cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Manfred B. Lutz, Shafaqat Ali, Cindy Audiger, Stella E. Autenrieth, Luciana Berod, Venetia Bigley, Laura Cyran, Marc Dalod, Jan Doerrie, Diana Dudziak, Georgina Florez-Grau, Lucila Giusiano, Gloria J. Godoy, Marion Heuer, Anne B. Krug, Christian H. K. Lehmann, Christian T. Mayer, Shalin H. Naik, Stefanie Scheu, Gerty Schreibelt, Elodie Segura, Kristin Sere, Tim Sparwasser, Jurjen Tel, Huaming Xu, Martin Zenke
Summary: This article is part of a series of guidelines on dendritic cells, providing advanced protocols for the preparation and characterization of murine and human dendritic cells. The protocols cover the preparation and generation of dendritic cells from different cellular sources, including the generation of different subsets such as cDC1, cDC2, and pDC using Flt3L in mice, and the generation of bone marrow-derived dendritic cells using GM-CSF. For human dendritic cells, the protocols focus on the culture of CD34(+) cells on OP9 cell layers to generate different subsets, as well as the generation of dendritic cells from peripheral blood monocytes. The article has been peer-reviewed and approved by the co-authors, making it an essential resource for basic and clinical dendritic cell immunologists.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Infectious Diseases
Flaminia Mazzone, Viktor E. Simons, Lasse van Geelen, Marian Frank, Attila Mandi, Tibor Kurtan, Klaus Pfeffer, Rainer Kalscheuer
Summary: Toxoplasma gondii is a pathogen that can infect various warm-blooded animals, including humans, causing toxoplasmosis. Current treatments for toxoplasmosis have severe side effects and are not effective in eradicating chronic infection. Therefore, there is an urgent need to develop novel anti-toxoplasma drugs with low toxicity. In this study, eight natural products were isolated from the crude extract of the endophytic fungus Paraboeremia selaginellae obtained from the leaves of the plant Philodendron monstera. Six of these natural products showed antitoxoplasma activity with low or no cytotoxicity in human cell lines. These findings suggest that biphenyl ethers, bioxanthracenes, and 5S,6S-phomalactone from P. selaginellae are potential candidates for novel anti-toxoplasma drugs.
Meeting Abstract
Immunology
Xiaoli Yang, Lisa Richter, Stefanie Scheu
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Meeting Abstract
Immunology
Shafaqat Ali, Ritu Mann Nuettel, Regine Dress, Patrick Petzsch, Karl Ehrich Koehrer, Haifeng Chris Xu, Philipp Lang, Judith Alferink, Stefanie Scheu
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Meeting Abstract
Immunology
Marcel Marson, Christina Ruland, Judith Alferink, Stefanie Scheu
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Genetics & Heredity
Ritu Mann-Nuettel, Shafaqat Ali, Patrick Petzsch, Karl Koehrer, Judith Alferink, Stefanie Scheu
Summary: This study reveals the role of transcription factors (TFs) in immune responses, particularly the less noticed but potentially important AP-1 family, in pDCs. The research demonstrates the involvement of TLR9-induced TFs in epigenetic modulation, NF kappa B and JAK-STAT signaling, and protein production in the endoplasmic reticulum.