Article
Chemistry, Applied
Feng Li, Yang-Yang Zhang, Tian-Zhen Liu, Bing-Yu Wei, Jun-Ying Miao, Bao-Xiang Zhao, Zhao-Min Lin
Summary: SO2, an important gaseous signaling molecule in cells, is also used as a preservative in food and drinks. A fluorescent probe CPO was developed to detect SO2 derivatives in body and food, showing high energy transfer efficiency and selectivity. It had fast response time and low detection limit, and could detect SO2 derivatives in various substances.
Article
Chemistry, Analytical
Lei Zhang, Jun Guo, Qihua You, Yuqing Xu
Summary: Intracellular pH plays a crucial role in cellular processes, and a water-soluble fluorescent pH probe based on the protonation/deprotonation of the 4-methylpiperazin-1-yl group was designed. The probe showed fluorescence OFF-ON mechanism under acidic conditions, exhibiting high selectivity, photostability, fast response to pH changes, and low cytotoxicity to cells. It can selectively accumulate in lysosomes and monitor pH changes stimulated by chloroquine in living cells. The probe has potential for diagnosing pH-related diseases.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Applied
Zhang-Yi Li, Xiao-Ling Cui, Meng-Min Xiao, Jun-Ying Miao, Bao-Xiang Zhao, Zhao-Min Lin
Summary: BDMNP is a novel FRET-ICT-based pH probe that has shown high energy transfer efficiency and linear response in the pH range of 5.4-6.8 (pKa = 6.10), with high selectivity and sensitivity. It has been successfully applied to monitor lysosomal pH changes in living cells and can detect subtle pH changes in various environmental water samples.
Article
Polymer Science
Koushik Bhattacharya, Moumita Kundu, Subhayan Das, Sarthik Samanta, Sib Sankar Roy, Mahitosh Mandal, Nikhil K. Singha
Summary: A functional fluorescent sensor has been developed in this study, which can distinguish cancer cells from normal cells by monitoring the pH change.
MACROMOLECULAR RAPID COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Jie Cui, Hailiang Nie, Xiaomeng Liang, Jie Bai, Xiaoling Zhang
Summary: Acidity in biological microenvironments plays a crucial role in physiology and pathology. This study developed a pyrene-derived ratiometric fluorescent probe that can sensitively and selectively monitor pH changes.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Analytical
Yong-Shun Chen, Bang-Zhao Zhou, Feng-Ting Liu, Jun-Ying Miao, Bao-Xiang Zhao, Zhao-Min Lin
Summary: A novel fluorescent probe cys-pH based on intramolecular charge transfer mechanism is developed for pH detection targeting mitochondria, with excellent mitochondrial targeting and sensitive response to fluctuations in mitochondrial pH.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Spectroscopy
Zhang-Yi Li, Meng-Min Xiao, Yi Zheng, Bao-Xiang Zhao
Summary: In this work, a new FRET-ICT-based ratiometric fluorescent and colorimetric probe (DMNP) was developed for detecting thiophenol. The probe exhibited a fast response, ultrahigh energy transfer efficiency, and clear spacing of emission peaks, and showed good performance in various real water samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Zhongjiao Feng, Jiasheng Wu, Meiyu Jiang, Jie Sha, Weimin Liu, Haohui Ren, Wenjun Zhang, Chun-Sing Lee, Pengfei Wang
Summary: The pH homeostasis of Golgi is crucial for maintaining normal cell functions. The development of an effective Golgi-targeting fluorescent probe (Golgi-hNR) that can sensitively detect pH changes is of great significance. By using Golgi-hNR, real-time tracking of Golgi pH variations in living cells has been successfully visualized.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Applied
Feng Li, Bang-Zhao Zhou, Shou-Kang Sun, Qi-Hong Yang, Jun-Ying Miao, Bao-Xiang Zhao, Zhao-Min Lin
Summary: In this study, a novel FRET-based fluorescent probe (named CBC) was developed for highly selective detection of bisulfite using a ratiometric method. Probe CBC showed excellent sensitivity towards HSO3- within 1 equivalent. Cell imaging experiments demonstrated that CBC could detect exogenous and endogenous bisulfite with excellent photostability and low cytotoxicity. Moreover, CBC probe achieved specific targeting to lipid droplets, indicating its potential as a biological tool for monitoring in vivo bisulfite changes.
Article
Chemistry, Analytical
Hongkang Xu, Yingcui Bu, JunJun Wang, Min Qu, Jie Zhang, Xiaojiao Zhu, Gang Liu, Zhichao Wu, Guangmei Chen, Hongping Zhou
Summary: Lyso-pH, a coumarin-based fluorescent molecule, shows excellent pH sensitivity and targeting capability for monitoring intracellular pH changes, particularly providing important insights into the interaction between mitochondria and lysosome.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Spectroscopy
Tian-Ran Wang, Qian Chen, Meng-Yu Tang, Yu Zhang, Shi-Li Shen, Xiao-Qun Cao
Summary: The article presents a near-infrared pH probe designed based on the rhodamine framework for detecting mitochondrial pH changes during Mitophagy. The probe shows high sensitivity to pH, suitable for mitochondrial pH detection, with excellent mitochondria-targeting ability.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Shan Wang, Bingya Wang, Lei Zhu, Ji-Ting Hou, Kang-Kang Yu
Summary: The ratiometric fluorescent probe presented in this study offers excellent photostability and chemostability for monitoring pH changes in living cells. Quantifying the down-regulation of intracellular pH during autophagy induced by various stimuli can provide valuable guidance for constructing autophagy models to study related pathological processes.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Yan Zhang, Ahui Qin, Shuai Gong, Mingxin Li, Zhiyuan Meng, Yueyin Liang, Zheyu Shen, Zhonglong Wang, Shifa Wang
Summary: Viscosity and pH are crucial microenvironment parameters that can indicate various diseases when experiencing abnormal fluctuations at the cellular level. Traditional viscosity/pH meters are not suitable for cellular environments, so there is an urgent demand for developing viscosity/pH-sensitive fluorescent probes. The novel dual-functional camphor-based fluorescent probe PN-SP exhibits intense green emission in response to alkaline pH and bright red fluorescence in high-viscosity media, offering a solution to problems caused by the use of multiple probes in cellular environments.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Hana Valenta, Sophie Dupre-Crochet, Mouna Abdesselem, Tania Bizouarn, Laura Baciou, Oliver Nusse, Ariane Deniset-Besseau, Marie Erard
Summary: The phagocyte NADPH oxidase is a key enzyme of the innate immune system, and the use of the chimeric protein Trimera can activate the enzyme and sustain high levels of activity, but it leads to intracellular acidification, apoptosis, and local peroxidation of lipids in the membrane.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Chemistry, Analytical
Yuxiang Zhao, Yabei Li, Shuake Kuermanbayi, Yulin Liu, Junjie Zhang, Zhaoyang Ye, Hui Guo, Kai Qu, Feng Xu, Fei Li
Summary: Ferroptosis, a promising therapeutic strategy for cancers, has attracted great interest. Monitoring the dynamic changes in key parameters during the early course of ferroptosis can provide insights for understanding the underlying mechanisms and developing targeted therapies.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Upendar G. Reddy, Jingjing Liu, Patrick Hoffmann, Johannes Steinmetzer, Helmar Goerls, Stephan Kupfer, Sven H. C. Askes, Ute Neugebauer, Stefanie Graefe, Alexander Schiller
Article
Biochemistry & Molecular Biology
Dirk Hubmacher, Nandaraj Taye, Zerina Balic, Stetson Thacker, Sheila M. Adams, David E. Birk, Ronen Schweitzer, Suneel S. Apte
Article
Chemistry, Multidisciplinary
Vadde Ramu, Gandra Upendar Reddy, Jingjing Liu, Patrick Hoffmann, Rudrakant Sollapur, Ralf Wyrwa, Stephan Kupfer, Christian Spielmann, Sylvestre Bonnet, Ute Neugebauer, Alexander Schiller
CHEMISTRY-A EUROPEAN JOURNAL
(2019)
Article
Chemistry, Inorganic & Nuclear
Jingjing Liu, Patrick Hoffmann, Johannes Steinmetzer, Sven H. C. Askes, Stephan Kupfer, Helmar Goerls, Stefanie Graefe, Ute Neugebauer, Upendar Reddy Gandra, Alexander Schiller
Article
Polymer Science
Upendar Reddy Gandra, Santhosh Kumar Podiyanachari, Antsar R. Hlil, Ihor Kulai, Saeed AI-Meer, Mohammed Al-Hashimi, Hassan S. Bazzi
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
(2019)
Article
Nanoscience & Nanotechnology
Upendar Reddy Gandra, Alessandro Sinopoli, Salvador Moncho, Manjula NandaKumar, Dragan B. Ninkovic, Snezana D. Zaric, Muhammad Sohail, Saeed Al-Meer, Edward N. Brothers, Nayef A. Mazloum, Mohammed Al-Hashimi, Hassan S. Bazzi
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Orthopedics
Nandaraj Taye, Stylianos Z. Karoulias, Dirk Hubmacher
JOURNAL OF ORTHOPAEDIC RESEARCH
(2020)
Article
Oncology
Aftab Alam, Nandaraj Taye, Sonal Patel, Milind Thube, Jayati Mullick, Vibhuti Kumar Shah, Richa Pant, Tanaya Roychowdhurey, Nilanjan Banerjee, Subhranosu Chatterjee, Rittwika Bhattachatya, Rini Roy, Ashis Mukhopadhyay, Devraj Mogare, Samit Chattopadhyay
Article
Multidisciplinary Sciences
Nandaraj Taye, Sarah Stanley, Dirk Hubmacher
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Biochemistry & Molecular Biology
Apoorva Parulekar, Arpankumar Choksi, Nandaraj Taye, Kumar V. S. Totakura, Priyanka Firmal, Gopal C. Kundu, Samit Chattopadhyay
Summary: The study reveals that down-regulation of SMAR1 leads to the activation of hTERT, resulting in an increase in the cancer stem cell phenotype in colorectal cancer cells. SMAR1 acts as a transcriptional repressor of hTERT, and its depletion promotes the total CD133(+)CD44(+) population and enhanced sphere-forming ability of colorectal cancer cells.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Keron W. J. Rose, Nandaraj Taye, Stylianos Z. Karoulias, Dirk Hubmacher
Summary: ADAMTS proteases play crucial roles in ECM formation and remodeling, but can also contribute to diseases such as arthritis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Upendar Reddy Gandra, Santhosh Kumar Podiyanachari, Hassan S. Bazzi, Mohammed Al-Hashimi
Summary: This mini-review presents recent advances in the synthesis of functional materials using ring-opening metathesis polymerization (ROMP)-derived poly(olefins) for drug release, sensing, and cellular uptake in the past seven years (2015-2022). The review highlights the highly promising potential of ROMP-derived well-defined functional poly(olefins) for medical and biological studies. It provides valuable insights for the design and development of new smart and flexible-functionality ROMP-based polymeric materials for various biological applications.
Article
Biochemistry & Molecular Biology
Nandaraj Taye, Mukti Singh, Clair Baldock, Dirk Hubmacher
Summary: Myogenesis is regulated by MYOD1 and involves ADAMTSL2, which acts as a signal hub to integrate WNT, TGF6, and potentially other signaling pathways in the dynamic microenvironment of differentiating myoblasts during skeletal muscle development and regeneration. ADAMTSL2 depletion leads to impaired myoblast differentiation and abnormal skeletal muscle architecture. Mechanistically, ADAMTSL2 enhances WNT signaling by binding to WNT ligands and receptors, and a WNT-binding ADAMTSL2 peptide is sufficient to promote myogenesis in vitro.
Article
Cell & Tissue Engineering
B. Satz-Jacobowitz, N. Taye, S. Z. Karoulias, D. Hubmacher
Summary: Biochemical and biophysical factors are important to consider when modeling in vivo cellular behavior in vitro. One underappreciated factor is the high concentration of macromolecules present in vivo, which is typically not simulated in standard cell culture conditions. This study found that adding inert macromolecules to cell culture medium can better mimic the high concentration environments found in vivo, leading to enhanced deposition of fibrillin-1.
EUROPEAN CELLS & MATERIALS
(2022)
Article
Multidisciplinary Sciences
Upendar Reddy Gandra, Raphael Courjaret, Khaled Machaca, Mohammed Al-Hashimi, Hassan S. Bazzi
SCIENTIFIC REPORTS
(2020)
