Article
Gastroenterology & Hepatology
Xian Li, Yi-Ru Han, Xuefeng Xuefeng, Yong-Xiang Ma, Guo-Sheng Xing, Zhi-Wen Yang, Zhen Zhang, Lin Shi, Xin-Lin Wu
Summary: This study suggests a potential role of CENPK in promoting tumor proliferation and it may serve as a novel diagnostic and prognostic biomarker for CRC.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Virology
Hemalatha Beesetti, Sathyamangalam Swaminathan
Summary: In this study, a HuAd5 vector encoding shRNA was shown to effectively suppress the replication of all four prototypic DENV serotypes in Vero cells and human macrophages. The HuAd5 vector-mediated DENV suppression in macrophages was found to be dependent on the presence of anti-HuAd5 antibodies, suggesting implications for the specific targeting of HuAd5 vector-mediated antiviral RNA interference therapy to macrophages.
Article
Agriculture, Dairy & Animal Science
Jun Gu, Hao Li, Zhen Bi, Kai Li, Zhiquan Li, Deping Song, Zhen Ding, Houjun He, Qiong Wu, Dongyan Huang, Ping Gan, Yu Ye, Yuxin Tang
Summary: The study shows that a dual shRNA expression system is an effective strategy in combating Porcine deltacoronavirus (PDCoV) infection without cytotoxicity, which would facilitate the ongoing development of RNAi-based therapeutic drugs against viral diseases.
Article
Chemistry, Analytical
Su Jiang, Qian Liu, Wen-Jing Liu, Lin Cui, Chun-Yang Zhang
Summary: A label-free fluorescent method has been developed to sensitively detect lncRNA in cancer cells. This method exhibits high sensitivity and potential for detecting different types of lncRNAs.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Medicine, Research & Experimental
Jin Qian, Bauer L. LeSavage, Kelsea M. Hubka, Chenkai Ma, Suchitra Natarajan, Joshua T. Eggold, Yiren Xiao, Katherine C. Fuh, Venkatesh Krishnan, Annika Enejder, Sarah C. Heilshorn, Oliver Dorigo, Erinn B. Rankin
Summary: Ovarian cancer-associated mesothelial cells play a critical role in promoting chemoresistance and stemness in ovarian cancer. The secreted factor osteopontin drives mesothelial-mediated chemoresistance and stemness, and therapeutic targeting of osteopontin may enhance platinum sensitivity in ovarian cancer.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Yoshinobu Kariya, Midori Oyama, Yukiko Kariya, Yasuhiro Hashimoto
Summary: Phosphorylated OPN secreted from cancer cells plays a regulatory role in cancer cell motility, with higher OPN expression levels correlating with increased cell migration capability. The presence of phosphorylated OPN in cell culture media affects cancer cell motility, indicating a potential mechanism for regulating cancer cell migration through the secretion of phosphorylated OPN.
Article
Pharmacology & Pharmacy
Kiruthiga Chandramohan, Devasahayam Jaya Balan, Kasi Pandima Devi, Seyed Fazel Nabavi, Sara Reshadat, Maryam Khayatkashani, Sepideh Mahmoodifar, Rosanna Filosa, Niloufar Amirkhalili, Soroush Pishvaei, Omolbanin Sargazi-Aval, Seyed Mohammad Nabavi
Summary: Colorectal cancer is a common and deadly disease, especially among older individuals. Chemotherapy has negative effects on both normal and malignant cells. Various signaling pathways, such as Hh, JAK/STAT, Wnt/beta-catenin, TNF-beta, EGFR/MAPK, PI3K, NF-kappa B, and Notch, are involved in the development of colorectal cancer. This study focuses on innovative siRNA therapies and delivery methods for the safe and effective treatment of colorectal cancer.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Nasir Uddin, Daniel W. Binzel, Dan Shu, Tian-Min Fu, Peixuan Guo
Summary: Exosome is a vesicle that can efficiently deliver therapeutics to the cytosol without endosome trapping, making it an excellent vehicle for in vivo therapy. However, its lack of specific recognition ability for target cells may cause potential side-effects and toxicity. Modification techniques have been used to decorate exosomes with targeting ligands and reduce nonspecific binding, providing a promising approach for targeted delivery of anticancer therapeutics.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Cell Biology
Yibin Zhu, Chi Zhang, Liming Zhang, Yun Yang, Xi Yu, Jinglin Wang, Qiyong Liu, Penghua Wang, Gong Cheng
Summary: This study reveals how a human-blood-derived microRNA, hsa-miR-150-5p, interferes with the mosquito's antiviral system to facilitate flavivirus infection and transmission. By hijacking the mosquito's RNA interference system, this microRNA suppresses the expression of certain chymotrypsins with potent virucidal activity, impacting the transmission of arboviruses.
Article
Biochemistry & Molecular Biology
Julien Ouvrard, Lisa Muniz, Estelle Nicolas, Didier Trouche
Summary: siRNAs can induce transcriptional silencing of chromatin-associated noncoding RNA through base-pairing with the target sequence. Transcriptional silencing occurs only in cis, and both Ago1 and Ago2 are involved.
MOLECULAR AND CELLULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Heike Braun, Michael Hauke, Anne Ripperger, Christian Ihling, Matthew Fuszard, Robert Eckenstaler, Ralf A. Benndorf
Summary: Knockdown of DICER1 or DROSHA by RNAi does not profoundly affect the angiogenic capacity of HUVEC, suggesting a potential increase in certain angiogenic activities instead. Further studies are needed to clarify the influence of these enzymes in the context of human endothelial cell-related angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Made Angga Akwiditya, Chean Yeah Yong, Mohd Termizi Yusof, Abdul Razak Mariatulqabtiah, Kok Lian Ho, Wen Siang Tan
Summary: Gene therapy using tHBcAg VLP as a carrier for shRNA targeting anti-apoptotic Bcl-2 has shown promising results in downregulating Bcl-2 expression in cancer cells. The encapsidated plasmid conjugated with folic acid demonstrated targeted delivery and significant reduction in cell viability, indicating the potential of this approach for gene silencing in cancer therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Bridget P. Keenan, Elizabeth E. McCarthy, Arielle Ilano, Hai Yang, Li Zhang, Kathryn Allaire, Zenghua Fan, Tony Li, David S. Lee, Yang Sun, Alexander Cheung, Diamond Luong, Hewitt Chang, Brandon Chen, Jaqueline Marquez, Brenna Sheldon, Robin K. Kelley, Chun Jimmie Ye, Lawrence Fong
Summary: Research has found that CD14+ monocytes increase in patients with anti-PD-1 refractory biliary tract cancer, leading to T cell dysfunction. The CD14CTX gene signature is associated with poorer prognosis.
Article
Agriculture, Multidisciplinary
Jui-Feng Tsai, Ting-Shuan Wu, Ying-Tzu Huang, Wan-Ju Lin, Feng-Yih Yu, Biing-Hui Liu
Summary: The mycotoxin citrinin (CTN) can induce chromosomal instability and has carcinogenic potential in human cells. Short-term and long-term exposure to CTN can promote cell migration, anchorage-independent growth, and mitotic spindle abnormalities.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jerome Laisney, Vanessa Loczenski Rose, Kayla Watters, Kevin Donohue, Jason M. Unrine
Summary: Research has shown that utilizing cerium oxide nanoparticles as a carrier successfully achieved oral delivery of shRNA, triggering RNA interference in stink bugs. By optimizing the properties of nanoparticles and the loading ratio of shRNA, successful reduction in target gene transcription levels was achieved, demonstrating the potential of nanoparticle delivery system to enhance the delivery of RNA-based biocontrols to stink bugs.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)