Article
Optics
Jiajia Chen, Zhenhong Du, Chenhao Xu, Xiao Xiao, Wei Gong, Ke Si
Summary: Here we report an ultrafast 3D histological imaging scheme based on optimized selective plane illumination microscopy (mSPIM), a minutes-time scale clearing method (FOCM), and a deep learning-based image enhancement algorithm (SRACNet) to realize histological preparation and imaging of clinical tissues. Our scheme enables fast imaging at micron-level resolution and demonstrates detailed 3D morphological connections that are difficult to identify in conventional 2D histology.
Article
Optics
Pablo Roldan-Varona, Calum A. A. Ross, Luis Rodriguez-Cobo, Jose Miguel Lopez-Higuera, Erin Gaughan, Kevin Dhaliwal, Michael G. G. Tanner, Robert R. R. Thomson, Helen E. E. Parker
Summary: Imaging fibers are used to perform real-time fluorescence endomicroscopy, in vivo, in situ, with the goal of increasing diagnostic information for a plethora of organ systems and diseases. Widefield fiber endomicroscopy systems are simple, cost-effective, and come with fast image acquisition times. However, alternative approaches such as scanning systems produce higher contrast images with intrinsic optical sectioning, improving the visibility of histological features, albeit at the expense of simplicity, cost, and acquisition rate. We developed a selective plane illumination microscopy endoscopic fiber platform, consisting of an ultrafast laser fabricated end-cap, integrated with a polymer coherent fiber bundle, and an epifluorescence microscope. Polymer fibers are known to fluoresce when pumped with blue light, enhancing the background and noise in images. Our end-cap design circumvents this challenge. We demonstrate a reduction of out-of-focus features, along with improved contrast of in-focus features, in images of a tissue phantom. Moreover, we demonstrate the utility of our platform for endomicroscopy using a whole, ex vivo human lung model.
Article
Chemistry, Analytical
Clara Gomez-Cruz, Sonia Laguna, Ariadna Bachiller-Pulido, Cristina Quilez, Marina Canadas-Ortega, Ignacio Albert-Smet, Jorge Ripoll, Arrate Munoz-Barrutia
Summary: This manuscript presents a novel architecture of a single-plane illumination microscopy (SPIM) for high-resolution imaging of live processes at a cellular level. The custom-made microscope overcomes limitations of existing techniques, achieving high-speed acquisition capabilities, low phototoxicity, and low mechanical disturbances, and demonstrating excellent imaging performance in microfluidic devices.
Article
Biochemical Research Methods
Caroline Bissardon, Xacier Mermet, Clement Quintard, Federico Sanjuan, Yves Fouillet, Frederic Bottausci, Marie Carriere, Florence Rivera, Pierre Blandin
Summary: In this article, the authors present an original selective plane illumination fluorescence microscope that is dedicated to imaging biostructures similar to organ-on-chips in microfluidic chips. The microscope compromises between a relatively large field of view and moderate resolution to allow morphological analysis of volumetric samples at the cellular scale inside microfluidic chambers. The setup is based on a simple design built around the chip and its microfluidic environment, enabling 3D imaging inside the chip.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemical Research Methods
Cong T. S. Van, Chrysanthe Preza
Summary: This study introduces a regularized three-dimensional model-based restoration method with positivity constraint for processing 3D data from structured illumination microscopy systems. The proposed method provides axial super resolution and improves 3D resolution compared to standard methods.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Optics
Yile Sun, Hongfei Zhu, Lu Yin, Hanmeng Wu, Mingxuan Cai, Weiyun Sun, Yueshu Xu, Xinxun Yang, Jiaxiao Han, Wenjie Liu, Yubing Han, Xiang Hao, Renjie Zhou, Cuifang Kuang, Xu Liu
Summary: Fluorescence interference structured illumination microscopy (FI-SIM) is a new imaging modality that allows for high axial resolution imaging without the need for z-axis scanning. It can quickly acquire images with relatively low laser power density, making it suitable for observing fixed and live subcellular structures.
ADVANCED PHOTONICS
(2023)
Article
Multidisciplinary Sciences
Morteza J. Siavashani, Iman Naghedi, Vahid Abbasian, Ehsan A. Akhlaghi, Mohammad A. Charsooghi, Mohammad Taghi Tavassoly, Ali-Reza Moradi
Summary: The study introduces a new method for quantitative phase imaging of cells using scanning diffractometry, which is non-contact and vibration-immune, for acquiring 3D cell images. Through Fresnel diffractometry, precise measurements of cell displacement can be achieved and applied to imaging different cell types.
SCIENTIFIC REPORTS
(2021)
Article
Optics
S. R. Rinsa, Kripa Chitre, Aditya Kurup, Upendra Nongthomba, Srinivasa Murty Srinivasula, Mayanglambam Suheshkumar Singh
Summary: We present a novel optical-based microscopy imaging technology called simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM) imaging system. This system overcomes the longstanding challenge of non-destructively obtaining images of biological samples at different field of views and spatial resolutions simultaneously in real-time. The unique feature of independently controlling the magnification powers of individual images enables simultaneous observation of sub-microscopic details and entire structure, as well as spatio-temporal dynamics of functional activities of biological specimens. Experimental results demonstrate the significant impacts of this system in both technological implication and biological applications.
Article
Biochemical Research Methods
Bingying Chen, Bo-Jui Chang, Philippe Roudot, Felix Zhou, Etai Sapoznik, Madeleine Marlar-Pavey, James B. Hayes, Peter T. Brown, Chih-Wei Zeng, Talley Lambert, Jonathan R. Friedman, Chun-Li Zhang, Dylan T. Burnette, Douglas P. Shepherd, Kevin M. Dean, Reto P. Fiolka
Summary: Structured illumination microscopy (SIM) improves the spatial resolution of fluorescence microscopy, while light-sheet fluorescence microscopy (LSFM) avoids exciting out-of-focus fluorescence. By combining SIM with LSFM using oblique plane microscopy, high-resolution three-dimensional imaging with low phototoxicity can be achieved.
Article
Biochemical Research Methods
Qiuyuan Zhong, Anan Li, Rui Jin, Dejie Zhang, Xiangning Li, Xueyan Jia, Zhangheng Ding, Pan Luo, Can Zhou, Chenyu Jiang, Zhao Feng, Zhihong Zhang, Hui Gong, Jing Yuan, Qingming Luo
Summary: The development of LiMo technique and HD-fMOST method allows for high-throughput imaging of thick tissues and substantial improvement in neuronal morphology reconstruction. By achieving >30-fold lossless data compression at a voxel resolution of 0.32 x 0.32 x 1.00 mu m(3), real-time high-precision (95% accuracy) brain-wide 3D cell counting is made possible.
Article
Chemistry, Multidisciplinary
Baolei Liu, Jiayan Liao, Yiliao Song, Chaohao Chen, Lei Ding, Jie Lu, Jiajia Zhou, Fan Wang
Summary: The study introduces a method for multiplexed super-resolution imaging using lifetime-engineered upconversion nanoparticles, achieving a lateral resolution of 185 nm. By utilizing a deep learning algorithm, more accurate decoding compared to a numeric algorithm is achieved, with decoding accuracies above 93% for a three-channel super-resolution imaging.
NANOSCALE ADVANCES
(2021)
Article
Engineering, Electrical & Electronic
Alexandr Melnikov, Daniel P. -K. Lun
Summary: This paper presents a new approach to three-dimensional imaging using a moving fringe illumination pattern in Structured Light Illumination Microscopy (SIM). By extracting the spatial location of the fluorescent object from the temporal frequency of the emitted signal, accurate 3D image reconstruction is achieved. The use of the Minimum-norm method in the proposed MF SIM system improves the axial resolution beyond the classical 3D SIM approach.
IEEE PHOTONICS JOURNAL
(2022)
Article
Engineering, Electrical & Electronic
Panchun Gu, Zengxin Huang, Mi Ping, Wenshuang Li, Meng Xiang, Xizeng Feng, Dengfeng Kuang
Summary: This study presents a novel method to generate multiplane parallel light sheets with high resolution and low phototoxicity using an isosceles triangular array microstructure. Experimental validation and comparison with simulations were performed to evaluate the imaging quality, showing promising results for volumetric imaging in light sheet fluorescence microscopy.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Article
Optics
Fanghui Xu, Jiachen Zhang, Dongdong Ding, Wenjie Liu, Chi Zheng, Sijia Zhou, Youhua Chen, Cuifang Kuang
Summary: This study presents an electro-optic high-speed phase-shift super-resolution microscopy imaging system with various modes and optimized design, achieving improved frequency in real-time reconstruction.
Article
Optics
Rinsa Salahudeen Rafeeka, Aditya Kurup, Debasish Roy, Upendra Nongthomba, Srinivasa Murty Srinivasula, Mayanglambam Suheshkumar Singh
Summary: This paper presents a detailed design and instrumentation of an optical imaging system, sMx-SPIM, which can image biological specimens at different magnification levels simultaneously. The simultaneous magnified views assist in perceiving biological activities occurring over a short period of time.
OPTICAL ENGINEERING
(2022)
Article
Urology & Nephrology
Suman Ranjit, Kammi Henriksen, Alexander Dvornikov, Marco Delsante, Avi Rosenberg, Moshe Levi, Enrico Gratton
KIDNEY INTERNATIONAL
(2020)
Article
Chemistry, Analytical
Alexander Vallmitjana, Alexander Dvornikov, Belen Torrado, David M. Jameson, Suman Ranjit, Enrico Gratton
METHODS AND APPLICATIONS IN FLUORESCENCE
(2020)
Article
Multidisciplinary Sciences
Marlene Cervantes, Ignasi Forne, Suman Ranjit, Enrico Gratton, Axel Imhof, Paolo Sassone-Corsi
Review
Urology & Nephrology
Suman Ranjit, Luca Lanzano, Andrew E. Libby, Enrico Gratton, Moshe Levi
Summary: Fluorescence microscopy techniques, including confocal microscopy and super-resolution techniques, have played a crucial role in evaluating kidney function and pathology. These advancements have allowed for detailed assessment of kidney structure and function, providing exceptional insights into kidney dynamics.
NATURE REVIEWS NEPHROLOGY
(2021)
Article
Multidisciplinary Sciences
Carolina Magdalen Greco, Marlene Cervantes, Jean-Michel Fustin, Kakeru Ito, Nicholas Ceglia, Muntaha Samad, Jiejun Shi, Kevin Brian Koronowski, Ignasi Forne, Suman Ranjit, Jonathan Gaucher, Kenichiro Kinouchi, Rika Kojima, Enrico Gratton, Wei Li, Pierre Baldi, Axel Imhof, Hitoshi Okamura, Paolo Sassone-Corsi
Review
Biophysics
Leonel Malacrida, Suman Ranjit, David M. Jameson, Enrico Gratton
Summary: The phasor approach in fluorescence lifetime imaging has become a common method to analyze complex fluorescence signals in biological samples, providing insights into fundamental biological states and molecular interactions. It offers new avenues for disease characterization and diagnostics.
ANNUAL REVIEW OF BIOPHYSICS, VOL 50, 2021
(2021)
Article
Multidisciplinary Sciences
Gao Sheng, Hongyan Yuan, Lu Jin, Suman Ranjit, Julia Panov, Xun Lu, Moshe Levi, Robert I. Glazer
Summary: The study identified fibrosis as a crucial factor in tumor progression, and demonstrated that using the LXR agonist DMHCA could reduce fibrosis, improve immune surveillance, decrease tumor progression and enhance the efficacy of cancer therapy.
Review
Chemistry, Analytical
Belen Torrado, Leonel Malacrida, Suman Ranjit
Summary: The phasor approach improves the analysis of fluorescence lifetime imaging and hyperspectral fluorescence imaging by increasing analytical speed, reducing data dimensionality, and utilizing the reciprocity principle in the phasor space. It allows for the calculation of phasor coordinates and fractional intensity contributions from multiple fluorescent species, even in the presence of complex fluorescence lifetime decays.
Article
Biochemistry & Molecular Biology
Ryan Kurtz, Andrew Libby, Bryce A. Jones, Komuraiah Myakala, Xiaoxin Wang, Yichien Lee, Grace Knoer, Julia N. Lo Cascio, Michaela McCormack, Grace Nguyen, Elijah N. D. Choos, Olga Rodriguez, Avi Z. Rosenberg, Suman Ranjit, Christopher Albanese, Moshe Levi, Carolyn M. Ecelbarger, Blythe D. Shepard
Summary: SGLT2 inhibition has emerged as a new therapeutic target for patients with diabetes and kidney disease. This study using an obese TallyHo mouse model found that modulation of renal glucose handling can decrease circulating triglycerides and lipid accumulation in the liver, improve liver structure and function, and promote healthy adipose expansion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Jiman Kang, Jedson R. Liggett, Digvijay Patil, Suman Ranjit, Katrina Loh, Anju Duttargi, Yuki Cui, Kesha Oza, Brett S. Frank, DongHyang Kwon, Bhaskar Kallakury, Simon C. Robson, Thomas M. Fishbein, Wanxing Cui, Khalid Khan, Alexander Kroemer
Summary: ILC1 cells play a significant role in fatty liver and ischemia-reperfusion injury under conditions of high-fat diet.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Endocrinology & Metabolism
David Montefusco, Maryam Jamil, Melissa A. Maczis, William Schroeder, Moshe Levi, Suman Ranjit, Jeremy Allegood, Dipankar Bandyopadhyay, Reuben Retnam, Sarah Spiegel, L. Ashley Cowart
Summary: Men with NAFLD are more likely to progress to NASH and liver fibrosis than women. This study revealed a novel pathway involving estrogen-mediated cross-talk between hepatocytes and HSCs that may contribute to sex differences in NAFLD through the anti-fibrogenic function of the S1P/S1PR3 axis.
MOLECULAR METABOLISM
(2022)
Article
Immunology
Jedson R. Liggett, Jiman Kang, Suman Ranjit, Olga Rodriguez, Katrina Loh, Digvijay Patil, Yuki Cui, Anju Duttargi, Sang Nguyen, Britney He, Yichien Lee, Kesha Oza, Brett S. Frank, DongHyang Kwon, Heng-Hong Li, Bhaskar Kallakury, Andrew Libby, Moshe Levi, Simon C. Robson, Thomas M. Fishbein, Wanxing Cui, Chris Albanese, Khalid Khan, Alexander Kroemer
Summary: This study investigated the role of Type 1 Natural Killer T-cells (NKT1 cells) in hepatic ischemia-reperfusion injury (IRI) and found that hepatic steatosis exacerbates the damage. Through experiments and analysis, it was observed that a high-fat diet increases the frequency of NKT1 cells and enhances interferon-gamma expression. The study also demonstrated that oral NAC supplementation can improve liver function and glycolysis, while reducing interferon-gamma expression. Lipidomic analysis revealed that NAC downregulates the PPAR-gamma pathway.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Nadia Sarfraz, Emilia Moscoso, Therese Oertel, Harrison J. Lee, Suman Ranjit, Esther Braselmann
Summary: Visualizing RNA in live cells is crucial for understanding its dynamics and functions in complex cellular environments. Current efforts involve genetically fusing RNA aptamer tags to the RNA of interest and using fluorescent molecules for detection. The Riboglow-FLIM platform presented in this study offers a smaller tag and superior cell contrast compared to intensity-based detection, allowing for simultaneous visualization of multiple RNAs using different lifetime-based tags.
NATURE COMMUNICATIONS
(2023)
Article
Biophysics
Nadia Sarfraz, Harrison J. Lee, Morgan K. Rice, Emilia Moscoso, Luke K. Shafik, Eric Glasgow, Suman Ranjit, Ben J. Lambeck, Esther Braselmann
Summary: The study establishes a RNA tagging and imaging platform called Riboglow-FLIM for complex cellular imaging applications. By evaluating the FLIM capabilities, they were able to optimize the entire process and successfully visualize and detect the cell-to-cell heterogeneity of noncoding RNA in live zebrafish embryos.
BIOPHYSICAL REPORTS
(2023)
Article
Chemistry, Physical
Alexander Vallmitjana, Belen Torrado, Alexander Dvornikov, Suman Ranjit, Enrico Gratton
JOURNAL OF PHYSICAL CHEMISTRY B
(2020)