Article
Biochemistry & Molecular Biology
Ankoor Patel, Emmely E. Treffers, Markus Meier, Trushar R. Patel, Jorg Stetefeld, Eric J. Snijder, Brian L. Mark
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Microbiology
Jing-wen Lin, Chao Tang, Han-cheng Wei, Baowen Du, Chuan Chen, Minjin Wang, Yongzhao Zhou, Ming-xia Yu, Lu Cheng, Suvi Kuivanen, Natacha S. Ogando, Lev Levanov, Yuancun Zhao, Chang-ling Li, Ran Zhou, Zhidan Li, Yiming Zhang, Ke Sun, Chengdi Wang, Li Chen, Xia Xiao, Xiuran Zheng, Sha-sha Chen, Zhen Zhou, Ruirui Yang, Dan Zhang, Mengying Xu, Junwei Song, Danrui Wang, Yupeng Li, ShiKun Lei, Wanqin Zeng, Qingxin Yang, Ping He, Yaoyao Zhang, Lifang Zhou, Ling Cao, Feng Luo, Huayi Liu, Liping Wang, Fei Ye, Ming Zhang, Mengjiao Li, Wei Fan, Xinqiong Li, Kaiju Li, Bowen Ke, Jiannan Xu, Huiping Yang, Shusen He, Ming Pan, Yichen Yan, Yi Zha, Lingyu Jiang, Changxiu Yu, Yingfen Liu, Zhiyong Xu, Qingfeng Li, Yongmei Jiang, Jiufeng Sun, Wei Hong, Hongping Wei, Guangwen Lu, Olli Vapalahti, Yunzi Luo, Yuquan Wei, Thomas Connor, Wenjie Tan, Eric J. Snijder, Teemu Smura, Weimin Li, Jia Geng, Binwu Ying, Lu Chen
Summary: This study utilized an integrative approach to analyze mutations of the SARS-CoV-2 virus, identifying 35 recurrent variants of clinical importance, with one variant associated with severe clinical phenotypes. The findings suggest that mutations in Nsp1 may have implications for molecular diagnostics and drug design.
CELL HOST & MICROBE
(2021)
Article
Microbiology
Kristina Kovacikova, Marina Gorostiola Gonzalez, Rhian Jones, Juan Reguera, Alba Gigante, Maria-Jesus Perez-Perez, Gerhard Puerstinger, Julia Moesslacher, Thierry Langer, Lak Shin Jeong, Leen Delang, Johan Neyts, Eric J. Snijder, Gerard J. P. van Westen, Martijn J. van Hemert
Summary: This study compared the antiviral efficacy of nsP1 inhibitors and found that CHVB and MADTP series had similar modes of action while differing from the FHNA series. Molecular-docking studies revealed the binding sites and potential actions of different series of inhibitors within the nsP1 structure.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Immunology
Laura Solforosi, Harmjan Kuipers, Mandy Jongeneelen, Sietske K. Rosendahl Huber, Joan E. M. van der Lubbe, Liesbeth Dekking, Dominika N. Czapska-Casey, Ana Izquierdo Gil, Miranda R. M. Baert, Joke Drijver, Joost Vaneman, Ella van Huizen, Ying Choi, Jessica Vreugdenhil, Sanne Kroos, Adriaan H. de Wilde, Eleni Kourkouta, Jerome Custers, Remko van der Vlugt, Daniel Veldman, Jeroen Huizingh, Krisztian Kaszas, Tim J. Dalebout, Sebenzile K. Myeni, Marjolein Kikkert, Eric J. Snijder, Dan H. Barouch, Kinga P. Boszormenyi, Marieke A. Stammes, Ivanela Kondova, Ernst J. Verschoor, Babs E. Verstrepen, Gerrit Koopman, Petra Mooij, Willy M. J. M. Bogers, Marjolein van Heerden, Leacky Muchene, Jeroen T. B. M. Tolboom, Ramon Roozendaal, Boerries Brandenburg, Hanneke Schuitemaker, Frank Wegmann, Roland C. Zahn
Summary: A two-dose regimen of Ad26.COV2.S vaccine induced higher antibody responses in aged NHPs and provided better protection against SARS-CoV-2. Neutralizing antibody responses remained stable for at least 14 weeks in one-dose regimens, indicating good durability.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Microbiology
Rui Guo, Xingyu Yan, Yanhua Li, Jin Cui, Saurav Misra, Andrew E. Firth, Eric J. Snijder, Ying Fang
Summary: This study demonstrates that PRRSV nsp2TF interacts with the major arteriviral envelope proteins GP5 and M, promoting arterivirus assembly by antagonizing ubiquitination-dependent proteasomal degradation of these key viral structural proteins.
Article
Immunology
Joan E. M. van der Lubbe, Sietske K. Rosendahl Huber, Aneesh Vijayan, Liesbeth Dekking, Ella van Huizen, Jessica Vreugdenhil, Ying Choi, Miranda R. M. Baert, Karin Feddes-de Boer, Ana Izquierdo Gil, Marjolein van Heerden, Tim J. Dalebout, Sebenzile K. Myeni, Marjolein Kikkert, Eric J. Snijder, Leon de Waal, Koert J. Stittelaar, Jeroen T. B. M. Tolboom, Jan Serroyen, Leacky Muchene, Leslie van der Fits, Lucy Rutten, Johannes P. M. Langedijk, Dan H. Barouch, Hanneke Schuitemaker, Roland C. Zahn, Frank Wegmann
Summary: This study investigated the immunogenicity, protective efficacy, and potential for vaccine-associated enhanced respiratory disease (VAERD) mediated by Ad26.COV2.S in a Syrian hamster challenge model with the G614 spike SARS-CoV-2 variant. Results showed that higher vaccine doses elicited substantial neutralizing antibodies titers and provided complete protection against lung infection and pneumonia in over 80% of Syrian hamsters inoculated with SARS-CoV-2.
Article
Biotechnology & Applied Microbiology
Margo A. Brinton, Anastasia A. Gulyaeva, Udeni B. R. Balasuriya, Magda Dunowska, Kay S. Faaberg, Tony Goldberg, Frederick C. C. Leung, Hans J. Nauwynck, Eric J. Snijder, Tomasz Stadejek, Alexander E. Gorbalenya
Summary: The Arteriviridae family consists of enveloped RNA viruses that infect non-human mammals, causing diseases in some cases while remaining asymptomatic in others.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dorothee Bardiot, Laura Vangeel, Mohamed Koukni, Philippe Arzel, Marleen Zwaagstra, Heyrhyoung Lyoo, Patrick Wanningen, Shamshad Ahmad, Linlin Zhang, Xinyuanyuan Sun, Adrien Delpal, Cecilia Eydoux, Jean-Claude Guillemot, Eveline Lescrinier, Hugo Klaassen, Pieter Leyssen, Dirk Jochmans, Karolien Castermans, Rolf Hilgenfeld, Colin Robinson, Etienne Decroly, Bruno Canard, Eric J. Snijder, Martijn J. van Hemert, Frank van Kuppeveld, Patrick Chaltin, Johan Neyts, Steven De Jonghe, Arnaud Marchand
Summary: In the search for small-molecule antiviral drugs against SARS-CoV-2, a high-throughput screening identified a promising hit structure that showed activity against various beta-coronaviruses by interfering with viral entry. Further structure-activity relationship studies revealed that a specific pyridyl moiety and different heteroaromatic cycles were optimal, and structural diversity was tolerated by the alkoxy group.
Article
Immunology
Anna H. E. Roukens, Cilia R. Pothast, Marion Koenig, Wesley Huisman, Tim Dalebout, Tamar Tak, Shohreh Azimi, Yvonne Kruize, Renate S. Hagedoorn, Mihaela Zlei, Frank J. T. Staal, Fenna J. de Bie, Jacques J. M. van Dongen, Sesmu M. Arbous, Jaimie L. H. Zhang, Maaike Verheij, Corine Prins, Anne M. van der Does, Pieter S. Hiemstra, Jutte J. C. de Vries, Jacqueline J. Janse, Meta Roestenberg, Sebenzile K. Myeni, Marjolein Kikkert, Maria Yazdanbakhsh, Mirjam H. M. Heemskerk, Hermelijn H. Smits, Simon P. Jochems
Summary: Systemic immune cell dynamics during COVID-19 have been well-documented, but less is known about immune cells in the respiratory tract. This study characterized nasal and systemic immune cells in COVID-19 patients, showing increased inflammatory cells in the nasal mucosa during acute infection. After recovery, nasal immune cells mostly normalized, but SARS-CoV-2-specific CD8(+) T cells could persist for at least 2 months.
Review
Cell Biology
Brandon Malone, Nadya Urakova, Eric J. Snijder, Elizabeth A. Campbell
Summary: The molecular basis and complexity of coronavirus RNA-synthesizing machinery is still not fully understood. Recent research has focused on deciphering and understanding the structures, functions, and interactions of the subunits involved in SARS-CoV-2 replication and transcription. Both viral and host factors play a crucial role in coordinating RNA translation, replication, and transcription, making them potential targets for antiviral therapy.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Natacha S. Ogando, Priscila El Kazzi, Jessika C. Zevenhoven-Dobbe, Brenda W. Bontes, Alice Decombe, Clara C. Posthuma, Volker Thiel, Bruno Canard, Francois Ferron, Etienne Decroly, Eric J. Snijder
Summary: The study found that N7-MTase is a critical enzyme for betacoronavirus replication and essential for virus viability. By conducting mutagenesis experiments on betacoronavirus nonstructural protein 14, key residues of the catalytic pocket were identified, which could be targeted for designing inhibitors with potential pan-coronaviral activity spectrum.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Nadia Oreshkova, Sebenzile K. Myeni, Niraj Mishra, Irina C. Albulescu, Tim J. Dalebout, Eric J. Snijder, Peter J. Bredenbeek, Kai Dallmeier, Marjolein Kikkert
Summary: The study successfully generated a novel vaccine platform by inserting subunits from three different betacoronaviruses into the yellow fever virus 17D vaccine, which elicited good immune responses in mice. These YF17D replicon-based vaccines could be a promising and safe option for current and future emerging coronaviruses.
Article
Chemistry, Medicinal
Rostom Ahmed-Belkacem, Marcel Hausdorff, Adrien Delpal, Priscila Sutto-Ortiz, Agathe M. G. Colmant, Franck Touret, Natacha S. Ogando, Eric J. Snijder, Bruno Canard, Bruno Coutard, Jean-Jacques Vasseur, Etienne Decroly, Francoise Debart
Summary: This study focuses on the design and synthesis of inhibitors against SARS-CoV-2 enzymes involved in RNA capping, which are important for viral RNA stability, mRNA translation, and evasion of innate immunity. Seven compounds showed significant inhibitory activity, and the three most potent inhibitors stabilized the enzyme effectively.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Melissa Thaler, Clarisse Salgado-Benvindo, Anouk Leijs, Ali Tas, Dennis K. Ninaber, Jack L. Arbiser, Eric J. Snijder, Martijn J. van Hemert
Summary: The SARS-CoV-2 pandemic has created a demand for broad-spectrum antivirals to be prepared for future outbreaks. In addition to inhibiting the virus, therapy should aim to suppress pathogenic host responses. Clinical studies have shown a link between SARS-CoV-2 infection and pathogenic angiogenesis in the lungs, involving the upregulation of angiogenic factors like ANGPTL4. Propranolol, a beta-blocker, has been used to suppress abnormal ANGPTL4 expression in the treatment of hemangiomas. This study found that R-propranolol can inhibit the replication of SARS-CoV-2 and other coronaviruses, as well as suppress the upregulation of ANGPTL4.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Bob Boogaard, Ali Tas, Joep Nijssen, Freek Broeren, John van den Dobbelsteen, Vincent Verhoeven, Jip Pluim, Sing Dekker, Eric J. Snijder, Martijn J. van Hemert, Sander Herfst
Summary: Due to shortages caused by the SARS-CoV-2 pandemic, three models of facemasks were designed to meet FFP2 requirements and can be produced locally.
AEROSOL AND AIR QUALITY RESEARCH
(2021)