Article
Microbiology
Vicken Aknadibossian, Jose C. Huguet-Tapia, Victor Golyaev, Mikhail M. Pooggin, Svetlana Y. Folimonova
Summary: Citrus tristeza virus (CTV) is an economically important pathogen that causes various damaging symptoms on citrus plants, and different variants of CTV can have different effects on host growth. Through transcriptome analysis, researchers identified a small number of differentially expressed genes in the asymptomatic T36-infected trees, while a much larger number of differentially expressed genes were identified in the growth-restricting T68-1 infection. These findings provide insights into the mechanisms underlying the growth repression induced by severe CTV isolates in sweet orange trees.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Sung-Hwan Kang, Vicken Aknadibossian, Laxmi Kharel, Shachinthaka D. Dissanayaka Mudiyanselage, Ying Wang, Svetlana Y. Folimonova
Summary: A unique viral protein, p33, was found to specifically bind to a long non-coding RNA (LMT1) produced by Citrus tristeza virus (CTV), which is the first report in the field of plant viruses. This interaction was confirmed through a series of experiments, and the biological significance of the interaction between these two viral factors was discussed.
Article
Plant Sciences
Maryam Khalilzadeh, Kyle Clark Weber, Manjul Dutt, Choaa Amine El-Mohtar, Amit Levy
Summary: This study investigated the transcriptome changes in Citrus macrophylla trees infected with different Citrus tristeza virus mutants. The results showed that the expression of specific genes related to xylem differentiation, phloem and cambium development, and cell cycle re-entry were associated with the development of stem pitting symptoms. The findings suggest that phloem regeneration may contribute to the development of stem pitting symptoms.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Munmi Borah, Palash Deb Nath, Supratik Pal Chaudhury, Kajal Kumar Biswas, Basavaprabhu L. Patil, Andreas Voloudakis
Summary: Citrus tristeza virus (CTV) causes a devastating disease in citrus plants, resulting in significant yield losses and even plant death. This study demonstrates the potential use of exogenously applied dsRNA for controlling CTV, providing an alternative to transgenic RNAi. These findings have important implications for non-transgenic approaches to manage CTV in citrus crops.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mahan Ghafari, Peter Simmonds, Oliver G. Pybus, Aris Katzourakis
Summary: This study provides a predictive mechanistic model to explain the rate decay phenomenon of viruses and successfully applies it to re-estimate the date of diversification of hepatitis C virus genotypes, as well as the origin time of the most recent common ancestor of sarbecoviruses. By studying the evolutionary processes of viruses, it offers a new perspective for us to understand the origins of these viruses.
Article
Virology
Changning Wang, Chaoyun Chen, Yiqun Chen, Ke Zhong, Long Yi
Summary: This study has revealed that CTV in China originated from wild citrus in Hunan and Jiangxi. The spatial-temporal distribution and dispersal patterns of CTV have provided insights into the population and pandemic history of CTV, contributing to a better understanding of its spread and origin in China.
Article
Biotechnology & Applied Microbiology
Yong-Duo Sun, Lei Zhang, Svetlana Y. Folimonova
Summary: Plants have developed a complex immune system to defend against pathogens. A study found that Citrus macrophylla miraculin-like protein 2 (CmMLP2) employs a two-way strategy to resist Citrus tristeza virus (CTV) infection, including interrupting p33 distribution and inducing cellular stress to inhibit virus replication.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Artem N. Bondaryuk, Nina V. Kulakova, Olga I. Belykh, Yurij S. Bukin
Summary: The study evaluated the temporal signal and substitution rate of different subtypes of tick-borne encephalitis virus (TBEV). The analysis showed that the TBEV Siberian subtype lacked temporal structure and could not be used for substitution rate estimation without other TBEV subtypes. The common clade of TBEV had the lowest substitution rate among tick-borne flaviviruses, and the European subtype had the lowest rate within the common clade.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Dilip Kumar Ghosh, Amol Kokane, Sunil Kokane, Krishanu Mukherjee, Jigme Tenzin, Datta Surwase, Dhanshree Deshmukh, Mrugendra Gubyad, Kajal Kumar Biswas
Summary: This study detected a high incidence of citrus tristeza virus (CTV) infection in Mandarin orange samples from Bhutan using reverse transcription PCR (RT-PCR). Genetic variability among Bhutanese CTV variants was analyzed by sequencing four independent genomic regions and comparing with GenBank deposited sequences. The study identified unexpected diversity among the Bhutanese CTV variants and also discovered a resistant-breaking variant. The findings contribute to the understanding of CTV diversity and provide insights into pathogenicity assessments.
FRONTIERS IN MICROBIOLOGY
(2022)
Editorial Material
Biology
Debapriyo Chakraborty
Summary: The repeated emergence of similar variants of influenza virus is due to interactions between the virus's RNA segments.
Article
Entomology
Fengnian Wu, Mochi Huang, Eduardo G. P. Fox, Jiaquan Huang, Yijing Cen, Xiaoling Deng, Meirong Xu
Summary: Citrus tristeza virus (CTV) is a significant viral pathogen of citrus trees transmitted by several aphid species. This study shows that the Asian citrus psyllid D. citri is capable of acquiring and transmitting CTV, potentially serving as a vector for two major citrus diseases, Huanglongbing (HLB) and Citrus tristeza. The persistence and transmission ability of CTV in D. citri varied among different host plant species, with no transmission observed to orange jasmine plants.
Article
Plant Sciences
Yong-Duo Sun, Svetlana Y. Folimonova
Summary: The study revealed the development process of stem pitting in citrus plants infected with Citrus tristeza virus (CTV), showing that the virus invades protophloem, metaphloem, and unexpected metaxylem cells, disrupting the differentiation of xylem tracheary elements and leading to the formation of stem pits. This provides new insights into the role of xylem tissue in virus pathogenicity.
Article
Microbiology
Daniel Goncalves-Carneiro, Paul D. Bieniasz
Summary: The development of safe and effective vaccines against viruses is crucial for disease control. Recent advancements in DNA synthesis technology have fueled research efforts in generating attenuated viruses through synonymous mutations. Understanding the molecular mechanisms of attenuation by genetic recoding of viruses could provide insights for vaccine development.
Article
Horticulture
Melina C. da Silva, Patricia I. S. Pinto, Rui Guerra, Amilcar Duarte, Deborah M. Power, Natalia T. Marques
Summary: This study investigated the transcriptome changes in Citrus macrophylla in response to drought stress and identified thousands of differentially expressed transcripts. The results showed that drought stress can induce the up- and down-regulation of various genes in the stem tissue of Citrus macrophylla, some of which may be associated with drought tolerance in this species.
SCIENTIA HORTICULTURAE
(2023)
Article
Biochemistry & Molecular Biology
Talia Kustin, Adi Stern
Summary: RNA viruses exhibit a trend towards A-rich coding sequences during evolution, mainly influenced by selection pressures, which contribute to the advancement of genome evolution. Research findings suggest that single-stranded RNA viruses undergo selective pressure resulting in the presence of A-rich coding sequences in their genomes.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biochemical Research Methods
Goncalo Silva, Joshua Oyekanmi, Chukwuemeka K. Nkere, Moritz Bomer, P. Lava Kumar, Susan E. Seal
ANALYTICAL BIOCHEMISTRY
(2018)
Article
Virology
Chukwuemeka K. Nkere, Joshua O. Oyekanmi, Goncalo Silva, Moritz Bomer, Gabriel I. Atiri, Joseph Onyeka, Norbert G. Maroya, Susan E. Seal, P. Lava Kumar
ARCHIVES OF VIROLOGY
(2018)
Article
Plant Sciences
Moritz Bomer, Ajith I. Rathnayake, Paul Visendi, Steven O. Sewe, Juan Paolo A. Sicat, Goncalo Silva, P. Lava Kumar, Susan E. Seal
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2019)
Article
Plant Sciences
Goncalo Silva, Moritz Bomer, Ajith Rathnayake, Steven O. Sewe, Paul Visendi, Joshua O. Oyekanmi, Marian D. Quain, Belinda Akomeah, P. Lava Kumar, Susan E. Seal
Article
Biochemistry & Molecular Biology
Natalia T. Marques, Alexandra Filipe, Patricia Pinto, Jose Barroso, Helena Trindade, Deborah M. Power, Ana Cristina Figueiredo
CHEMISTRY & BIODIVERSITY
(2020)
Article
Horticulture
Melina C. da Silva, Patricia I. S. Pinto, Rui Guerra, Amilcar Duarte, Deborah M. Power, Natalia T. Marques
Summary: This study investigated the transcriptome changes in Citrus macrophylla in response to drought stress and identified thousands of differentially expressed transcripts. The results showed that drought stress can induce the up- and down-regulation of various genes in the stem tissue of Citrus macrophylla, some of which may be associated with drought tolerance in this species.
SCIENTIA HORTICULTURAE
(2023)
Article
Agronomy
Melina da Silva, Sandra Germano, Amilcar Duarte, Patricia Pinto, Natalia Tomas Marques
Summary: Citrus tristeza virus (CTV) has caused significant losses in citrus producing regions. This study investigates the effect of CTV infection on the expression of cell wall-associated genes in two citrus cultivars, ‘Valencia Late’ and ‘Fina’. The results suggest that CTV infection can modify the expression of specific genes in the cell wall, and this influence varies between cultivars.
Article
Plant Sciences
Susana A. Dandlen, Jose P. Da Silva, Maria Graca Miguel, Amilcar Duarte, Deborah M. Power, Natalia Tomas Marques
Summary: The susceptibility of Citrus macrophylla (CM) to the severe Citrus tristeza virus (CTV), T36, is higher than that of Citrus aurantium (CA). The impact of host-virus interactions on host physiology remains largely unknown. This study evaluated the metabolite profile and antioxidant activity in the phloem sap of healthy and infected CA and CM plants. The results showed that the antioxidant enzyme activity increased significantly in infected CM plants and decreased in infected CA plants. Furthermore, CTV infection resulted in a drastic reduction in secondary metabolites in CA but not in CM.
Review
Plant Sciences
Ana Rita Trindade, Paulo Paiva, Vander Lacerda, Natalia Marques, Luis Neto, Amilcar Duarte
Summary: Pitaya, or dragon fruit, has seen an increase in demand due to its health benefits and profitability. The Iberian Peninsula and Mediterranean countries have favorable climate for cultivating pitaya. Research shows that pitaya has relatively low water requirements, making it a sustainable crop. However, more research is needed to increase productivity and improve fruit quality in different regions.
Meeting Abstract
Biochemistry & Molecular Biology
S. A. Dandlen, M. P. Trindade, L. T. Anjos, A. M. Duarte, D. M. Power, N. T. Marques
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Review
Biochemistry & Molecular Biology
Goncalo Silva, Jenny Tomlinson, Nawaporn Onkokesung, Sarah Sommer, Latifa Mrisho, James Legg, Ian P. Adams, Yaiza Gutierrez-Vazquez, Thomas P. Howard, Alex Laverick, Oindrila Hossain, Qingshan Wei, Kaitlin M. Gold, Neil Boonham
Summary: Plant pests and diseases have increasingly impacted food security and natural ecosystems in recent years, due to factors such as globalisation, climate change, and pesticide resistance. Early detection and molecular diagnostics play a crucial role in controlling pests and diseases effectively.
EMERGING TOPICS IN LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Mathias Tembo, Adedapo O. Adediji, Sophie Bouvaine, Patrick C. Chikoti, Susan E. Seal, Goncalo Silva
SCIENTIFIC REPORTS
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
C. M. Varanda, P. Materatski, M. D. Campos, M. I. Clara, N. Marques, G. Nolasco, M. R. Felix
MOLECULAR PLANT-MICROBE INTERACTIONS
(2019)
Article
Virology
Moritz Bomer, Ajith I. Rathnayake, Paul Visendi, Goncalo Silva, Susan E. Seal
ARCHIVES OF VIROLOGY
(2018)
Article
Agriculture, Multidisciplinary
Andreia M. Afonso, Rui Guerra, Ana M. Cavaco, Patricia Pinto, Andre Andrade, Amilcar Duarte, Deborah M. Power, Natalia T. Marques
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2017)