Journal
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
Volume 50, Issue 2, Pages 360-366Publisher
SPRINGER INDIA
DOI: 10.1007/s13197-011-0345-9
Keywords
L. fermentum; HT-29; Caco-2; Fermented milk; Fructooligosaccharides; Vitamin B-12
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Funding
- CSIR
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Functional properties of the Lactobacillus fermentum CFR 2195, isolated from healthy infant feces have been evaluated. The adherence of L. fermentum to HT-29 and Caco-2 cell-lines were found to be 197.66 +/- 15.62 and 100.33 +/- 15.69 per 100 cells, respectively. The effect of different concentrations of FOS (0.5, 1.0, 1.5, 2.0 and 2.5%) on the growth rate of L. fermentum was checked and 2.0% FOS was selected for further studies. The synbiotic preparation containing L. fermentum and FOS exhibited significant antimicrobial activity against a few tested common food borne pathogens. The proteolytic activity of the L. fermentum was significant and the total amino acid content in milk fermented with L. fermentum was 555 mg/l. In addition, it was found to produce 29.45 ng vitamin B-12/g dry biomass in submerged fermentation (96 h) with successive anaerobic and aerobic phases of 48 h.
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