Article
Engineering, Environmental
Xiaofeng Yi, Shenhua Huang, Lu Chang, Zhaoshou Wang, Yuanpeng Wang
Summary: The study investigated As(V) immobilization and redistribution processes during ferrihydrite reduction mediated by Geobacter sulfurreducens. The results showed that As(V) was redistributed on different minerals and EPS induced by G. sulfurreducens, with the addition of TiO2 nanoparticles delaying rather than promoting the entire process. Changes in aqueous arsenic and iron concentrations were found to be closely related to the formation time of secondary minerals.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Jianping Wu, Shenhua Huang, JiaYing Su, Xiaofeng Yi, Yuanpeng Wang
Summary: This study demonstrated that the cooperation of Geobacter sulfurreducens and ferrihydrite can continuously fix the highly toxic As(III) released into the water, providing an environmentally friendly method to treat dissolved As(III) pollutants in soil.
Article
Microbiology
Ethan Howley, Dongwon Ki, Rosa Krajmalnik-Brown, Cesar Torres
Summary: This study analyzed the cell composition of Geobacter sulfurreducens using metallomics, cell fraction and elemental analyses, and transcriptomics. The results showed that G. sulfurreducens cells have high concentrations of iron and lipids, which do not change with different electron acceptors.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Xiaomeng Liu, Toshiyuki Ueki, Hongyan Gao, Trevor L. Woodard, Kelly P. Nevin, Tianda Fu, Shuai Fu, Lu Sun, Derek R. Lovley, Jun Yao
Summary: Using microbial biofilms as renewable materials for fabricating clean energy harvesting devices can generate continuous electricity from evaporating water, enhancing sustainability.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Jinxin Xie, Victoria S. Coker, Brian O'Driscoll, Rongsheng Cai, Sarah J. Haigh, Jonathan R. Lloyd
Summary: This study found that Geobacter sulfurreducens cannot directly reduce pentavalent antimony or cause its release. The results highlight the close associations between iron and antimony in environmental systems, suggesting the potential use of iron for remediation of antimony contamination.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yonggang Yang, Zegao Wang, Cuifen Gan, Lasse Hyldgaard Klausen, Robin Bonne, Guannan Kong, Dizhou Luo, Mathijs Meert, Chunjie Zhu, Guoping Sun, Jun Guo, Yuxin Ma, Jesper Tataru Bjerg, Jean Manca, Meiying Xu, Lars Peter Nielsen, Mingdong Dong
Summary: Long-distance extracellular electron transfer has been observed in Gram-negative bacteria and now also in a filamentous, unicellular Gram-positive bacterium. The filamentous bacterium Lysinibacillus varians GY32 is capable of bidirectional extracellular electron transfer, forming centimetre-range conductive cellular networks with 1mm-long cells and conductive appendages. Our results demonstrate that Gram-positive bacteria can also participate in long-distance electron transfer processes.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Mon Oo Yee, Lars D. M. Ottosen, Amelia-Elena Rotaru
Summary: Improving methane production through electrical current application to anaerobic digesters has garnered interest, but this study shows that electrochemical stimulation has an overall detrimental effect on a direct interspecies electron transfer (DIET) consortium. The presence of an alternative electron donor inhibits efficient electron retrieval by the methanogen from Geobacter and induces catabolic repression of oxidative metabolism in Geobacter.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Guoping Ren, Zhao Wang, Bintian Zhang, Xing Liu, Jie Ye, Qichang Hu, Shungui Zhou
Summary: The study introduces a simple whole-cell hygroelectric generator technology that generates sustainable electricity from ambient humidity. Results demonstrate that the energy harvesting performance is highly influenced by surface hydrophilicity and ionization level.
Article
Engineering, Environmental
Lili Tian, Xuejun Yan, Dongbin Wang, Qing Du, Yuxuan Wan, Lean Zhou, Tian Li, Chengmei Liao, Nan Li, Xin Wang
Summary: Electroactive biofilms, with the unique ability to exchange electrons with electrodes, have gained increasing attention. This study reveals that Geobacter spp., specifically G. sulfurreducens and G. anodireducens, respond differently to electric fields (EF), resulting in the enrichment of G. anodireducens in the strong EF region. Transcriptome analysis suggests that two-component systems are key for EF sensing in G. anodireducens, while also highlighting the involvement of chemotaxis, metabolism, secretion, and other processes in Geobacter biofilm formation.
Article
Environmental Sciences
Rasha Elmeihy, Xiao-Chen Shi, Pier-Luc Tremblay, Tian Zhang
Summary: Geobacter sulfurreducens can efficiently remove hexavalent chromium from contaminated sites by reducing it to less toxic chromium (III) with the help of extracellular proteins. The high-density cultures of G. sulfurreducens have shown great potential for bioremediation applications, such as the treatment of industrial wastewaters.
Article
Biology
Fengbin Wang, Chi Ho Chan, Victor Suciu, Khawla Mustafa, Madeline Ammend, Dong Si, Allon Hochbaum, Edward H. Egelman, Daniel R. Bond
Summary: Early genetic and structural observations suggested that metal-reducing organisms like Geobacter had extracellular conductive filaments composed of type IV pili. However, recent research has shown that bacterial c-type cytochromes can also form extracellular filaments for long-range electron transport. Cryo-electron microscopy was used to determine the structure of a third extracellular filament, formed from Geobacter sulfurreducens octaheme cytochrome, OmcZ. The structure of OmcZ differs from that of OmcS and OmcE, indicating that conductive cytochrome polymers likely arose independently from different ancestral multiheme proteins.
Review
Biochemical Research Methods
Elie Desmond-Le Quemener, Roman Moscoviz, Nicolas Bernet, Andrew Marcus
Summary: The study of mathematical modeling of DIET and MIET in anaerobic ecosystems offers new strategies for improving biotechnologies. While the modeling of DIET kinetics is still in its early stages, recent concepts introduced for modeling electroactive biofilms should be further developed. DIET shows potential to increase IET rates compared to H 2 -MIET, but additional characterizations of biological components supporting IET are needed to enhance predictions.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Tao Wang, Gefu Zhu, Bin Kuang, Jianbo Jia, Changyu Liu, Guanjing Cai, Chunxing Li
Summary: The co-culture of G. sulfurreducens and S. fumaroxidans in bioelectrochemical cells achieved more efficient propionate degradation compared to the control group. Carbon resource concentration and pH were both significantly correlated with propionate degradation. G. sulfurreducens strengthened the consumption of H-2 and acetate via direct interspecific electron transfer in propionate degradation.
Article
Microbiology
Hayley J. Gadol, Joseph Elsherbini, Benjamin D. Kocar
Summary: This research investigates the impact of different iron oxide minerals on microbial methane production and community development. It shows that only poorly crystalline iron oxide mineral, ferrihydrite, has a significant effect on iron reduction and microbial community composition. The presence of ferrihydrite continues to influence microbial community development even after the onset of methanogenesis, leading to increased dissimilarity compared to control groups.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Derek R. Lovley, Jun Yao
Summary: Intrinsically conductive protein nanowires produced from inexpensive, renewable feedstocks offer a sustainable alternative to traditional nanowire electronic materials, with potential for various applications in electronics without the need for high energy inputs or toxic chemicals. Further development of these protein nanowire electronics will require interdisciplinary teams of engineers, biophysicists, and synthetic biologists.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Yixin Li, Zheng Chen, Yanyan Shi, Qingliu Luo, Yiming Wang, Honghui Wang, Yajuan Peng, Haitao Wang, Ning He, Yuanpeng Wang
JOURNAL OF HAZARDOUS MATERIALS
(2020)
Article
Environmental Sciences
Xu Shang, Hong Huang, Kun Mei, Fang Xia, Zheng Chen, Yue Yang, Randy A. Dahlgren, Minghua Zhang, Xiaoliang Ji
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Review
Environmental Sciences
Guowen Dong, Honghui Wang, Zhiying Yan, Jing Zhang, Xiaoliang Ji, Maozi Lin, Randy A. Dahlgren, Xu Shang, Minghua Zhang, Zheng Chen
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Soil Science
Liuying Wang, Zhenyue Lin, Shurui Liu, Dun Fu, Zhipeng Li, Qingliu Luo, Jianwu Tang, Zheng Chen, Ning He, Yuanpeng Wang
SOIL & TILLAGE RESEARCH
(2020)
Article
Engineering, Environmental
Guowen Dong, Ruiwen Han, Yajing Pan, Chengkai Zhang, Yu Liu, Honghui Wang, Xiaoliang Ji, Randy A. Dahlgren, Xu Shang, Zheng Chen, Minghua Zhang
Summary: This study found that adding MnO2 particles under intermittent illumination significantly promoted the mobility of Fe and As in arsenic-enriched soils, with higher dosage demonstrating greater effects. MnO2 amendments not only altered the soil microbial community structure, but also enhanced the reduction of Fe/As.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Engineering, Environmental
Xiaoliang Ji, Yuan Ma, Ganning Zeng, Xiaoqun Xu, Kun Mei, Zhenfeng Wang, Zheng Chen, Randy Dahlgren, Minghua Zhang, Xu Shang
Summary: This study found that dredged sediments contain high concentrations of microplastics, which may potentially remobilize into the environment during the handling process. The dredged sediments stored in farmlands can disperse microplastics to surrounding soils and surface waters, with higher concentrations observed in dry and wet seasons due to wind dispersion and rainfall/runoff erosion, respectively.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Yue Yang, Xu Shang, Zheng Chen, Kun Mei, Zhenfeng Wang, Randy A. Dahlgren, Minghua Zhang, Xiaoliang Ji
Summary: A support vector regression (SVR) model was developed to predict nitrogen isotopic composition of nitrate in water bodies, outperforming multivariate linear regression and general regression neural network models in terms of accuracy.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Review
Environmental Sciences
Zheng Chen, Jing Zhang, Qingyang Lyu, Honghui Wang, Xiaoliang Ji, Zhiying Yan, Fang Chen, Randy A. Dahlgren, Minghua Zhang
Summary: This review provides a comprehensive assessment of recent advancements in the design of living biomaterials that can be exploited to enhance bioelectrocatalytic performance in bioelectrochemical systems. The integration of modular configurations of abiotic and biotic components, such as conductive nanomaterials and electrochemically active microbes, show promise in significantly boosting extracellular electron transfer efficiency. The interdisciplinary approach combining molecular biology, electrochemistry, and materials science is crucial in advancing the utility and novelty of bioelectrochemical systems and meeting global sustainability goals.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Mingzhu Fang, Zhonglu Liao, Xiaoliang Ji, Xuan Zhu, Zhenfeng Wang, Changjie Lu, Chenwei Shi, Zheng Chen, Liyun Ge, Minghua Zhang, Randy A. Dahlgren, Xu Shang
Summary: This study identified that ingestion of atmospheric-deposited microplastics during routine dining/drinking activities is an important exposure pathway, in addition to inhalation. The measured abundances of atmospheric-deposited microplastics were high, with a dominance of amorphous fragments. The ingestion of atmospheric-deposited microplastics through diet was found to be similar to inhalation exposure but significantly greater than direct ingestion from food sources. Simple mitigation strategies can effectively reduce the exposure of atmospheric-deposited microplastics through diet.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Engineering, Civil
Xiaoliang Ji, Lielin Shu, Jian Li, Congyuan Zhao, Wenli Chen, Zheng Chen, Xu Shang, Randy A. Dahlgren, Yue Yang, Minghua Zhang
Summary: This study used a multiple isotope approach to quantitatively identify sources and transformations of riverine nitrate in a wetland plain river network in eastern China. The results showed that municipal sewage was the dominant nitrate source, followed by soil nitrogen, atmospheric nitrate deposition, and nitrogen fertilizer. Additionally, the study highlighted the benefits of using Delta O-17-NO3- to enhance nitrate source apportionment.
JOURNAL OF HYDROLOGY
(2022)
Article
Engineering, Environmental
Siguang Liu, Yifeng Huang, Dehua Luo, Xiao Wang, Zhenfeng Wang, Xiaoliang Ji, Zheng Chen, Randy A. Dahlgren, Minghua Zhang, Xu Shang
Summary: The sinking of microplastics after biofouling is an important mechanism for their transport and sedimentation in oceans and freshwaters. Smaller microplastic particles sink faster, while films and fibers do not necessarily sink faster.
Article
Environmental Sciences
Jian Li, Feng Wang, Jing Zhang, Honghui Wang, Chongyuan Zhao, Lielin Shu, Peng Huang, Yejing Xu, Zhiying Yan, Randy A. Dahlgren, Zheng Chen
Summary: A novel photosensitized living biohybrid was created by assembling amine-functionalized carbon dots and polydopamine onto bacteria, which showed high efficiency in catalytic reduction of Cr(VI) under visible light. The modular configuration of the biohybrid allowed for increased electron flux and enhanced electron transport ability. Moreover, the carbon dots themselves also directly reduced Cr(VI).
Article
Engineering, Environmental
Xiaoliang Ji, Lielin Shu, Wenli Chen, Zheng Chen, Xu Shang, Yue Yang, Randy A. Dahlgren, Minghua Zhang
Summary: This study examined the sources and contributions of riverine nitrate in the Wen-Rui Tang River in China using various methods. The SIAR model confirmed municipal sewage as the major source of nitrate, with nitrogen fertilizer and soil nitrogen also identified as secondary sources. The study found that the uncertainties associated with different sources varied, and the isotopic composition of the predominant source had the strongest effect on the overall results of nitrate source apportionment.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Congyuan Zhao, Jing Zhang, Zheng Chen, Jian Li, Lielin Shu, Xiaoliang Ji
Summary: This paper summarizes effective constructions for improving electron transfer efficiency in bio-electrocatalytic systems and discusses the mechanisms for strengthening extracellular electron transfer. Additionally, it explores the challenges and opportunities for microbes-based living biomaterials in environmental applications.
PROGRESS IN CHEMISTRY
(2022)
Article
Environmental Sciences
Jian Li, Feng Wang, Jing Zhang, Honghui Wang, Chongyuan Zhao, Lielin Shu, Peng Huang, Yejing Xu, Zhiying Yan, Randy A. Dahlgren, Zheng Chen
Summary: A novel photosensitized living biohybrid was fabricated by inward-to-outward assembly of amine-functionalized carbon dots (NCDs) and polydopamine (PDA) to Shewanella oneidensis MR-1 for high-efficiency microbial-photoreduction of Cr(VI). Modular configurations of NCDs and PDA provided biohybrids with a large electron flux, enhancing electron transport ability and achieving significant reduction capacity for Cr(VI). The NCD-assembled biohybrids showed enhanced Cr(VI) reduction, outperforming conventional microbial technologies for Cr(VI) remediation.
