Article
Microbiology
Sergey N. Gavrilov, Daria G. Zavarzina, Ivan M. Elizarov, Tamara V. Tikhonova, Natalia I. Dergousova, Vladimir O. Popov, Jonathan R. Lloyd, David Knight, Mohamed Y. El-Naggar, Sahand Pirbadian, Kar Man Leung, Frank T. Robb, Maksim V. Zakhartsev, Orianna Bretschger, Elizaveta A. Bonch-Osmolovskaya
Summary: The biogenic transformation of Fe minerals associated with extracellular electron transfer (EET) by thermophilic Gram-positive bacteria is not well understood. In this study, the EET pathways in the thermophilic Gram-positive bacterium Carboxydothermus ferrireducens were investigated, revealing the formation of magnetite crystals and active colonization of anodes in bioelectrochemical systems. Genome analysis identified novel c-type multiheme cytochromes involved in electron exchange with ferrihydrite or an anode, providing insights into cell-to-mineral interactions in thermal environments.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Ola M. Gomaa, Nazua L. Costa, Catarina M. Paquete
Summary: Bioelectrochemical systems are a rising technology with versatile applications, but the use of Gram-positive bacteria has been limited due to a lack of knowledge about their electron transfer pathways and the presence of a non-conductive cell wall. This review explores the electroactivity of Gram-positive bacteria, focusing on different pathways of electron transfer and the roles of multiheme cytochromes, quorum sensing molecules, peptide-based signaling, and pili in extracellular electron transfer. Understanding these fundamentals is critical for improving the use of Gram-positive bacteria in bioelectrochemical systems and discovering new applications.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Ecology
Ugo Marzocchi, Casper Thorup, Ann-Sofie Dam, Andreas Schramm, Nils Risgaard-Petersen
Summary: The study reveals that the freshwater cable bacterium Ca. Electronema sp. GS is capable of dissimilatory nitrate reduction to ammonium (DNRA). This finding suggests that these bacteria may play a significant role in the nitrate cycle, contributing to higher rates of DNRA and nitrite production in the sediment.
Article
Microbiology
Sara Tejedor-Sanz, Siliang Li, Biki Bapi Kundu, Caroline M. Ajo-Franklin
Summary: We report the first evidence of electron uptake from a cathode by Lactiplantibacillus plantarum, a primarily fermentative bacteria, and its coupling to the reduction of organic and inorganic electron acceptors. This process reroutes glucose fermentation towards lactate degradation and enhances cell viability.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Tatsuki Kunoh, Tatsuya Yamamoto, Manoj Prasad, Erika Ono, Xiaojie Li, Shinya Sugimoto, Eiji Iida, Nozomu Obana, Minoru Takeda, Nobuhiko Nomura, Andrew S. S. Utada
Summary: The bacterium Leptothrix cholodnii forms porous pellicles at the air-liquid interface by swimming towards the interface using its polar flagellum and intertwining with others with the assistance of its nanofibril sheath.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Federico Turco, Marco Garavaglia, Rob Van Houdt, Phil Hill, Frankie J. Rawson, Katalin Kovacs
Summary: This study established and characterized inducible and constitutive promoter libraries for Cupriavidus metallidurans CH34 and developed a single-plasmid CRISPR-Cas9 system that can be delivered by both conjugation and electroporation. The CRISPR-Cas9 system was used to target candidate genes, resulting in successful generation of single and double deletion strains. The findings suggest that the deleted type IV pili candidate genes are not involved in extracellular electron transfer under specific conditions.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Microbiology
Abhiney Jain, Anaisa Coelho, Joana Madjarov, Catarina M. Paquete, Jeffrey A. Gralnick
Summary: Fe(II)-oxidizing bacteria are crucial in biogeochemical cycles and perform extracellular electron transfer through the Mto pathway. The electron transport proteins required for the growth of Sideroxydans species remain understudied, and there is debate about the functionality and directionality of these proteins.
Article
Thermodynamics
M. S. Pattanaik, S. K. Cheekati, V. B. Varma, R. Ramanujan
Summary: Heat transfer efficiency is a major challenge in many industrial and commercial systems, where conventional heat pipes are limited by their heat transport performance. A magnetic cooling device based on ferrofluid technology can transfer heat over longer distances, exhibiting self-pumping and self-regulating characteristics. Enhanced cooling is achieved near strong magnetic fields, with Nusselt number showing maximum values.
APPLIED THERMAL ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Shuomeng Zhang, Lei Wang, Liang Wu, Zhongjian Li, Bin Yang, Yang Hou, Lecheng Lei, Shaoan Cheng, Qinggang He
Summary: The study investigates the adhesion behavior of electroactive bacteria under various metabolic conditions using an integrated electrochemical single-cell force microscopy system. It establishes a nonlinear model between bacterial adhesion force and electric field intensity, and demonstrates how the EET processes impact the conformation and unfolding events of outer membrane appendages. The research reveals how the anisotropy of bacterial conductive structure translates into desired adhesion behavior in different scenarios.
Article
Microbiology
Rohit Ruhal, Rashmi Kataria
Summary: Biofilms formed by Gram-positive and Gram-negative bacteria are the root cause of industrial biofouling and antimicrobial resistance during infections. To understand the mechanisms of biofilm formation better, it is essential to study common components and genetic regulations that are shared among the two types of bacteria.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Immunology
Qingliang Wang, Xiaojie Li, Wenting Tang, Xiaoling Guan, Zhiyong Xiong, Yong Zhu, Jiao Gong, Bo Hu
Summary: This study explored the gene differences between Gram-negative, Gram-positive, and mixed infections in sepsis using genome-wide techniques. The results showed molecular heterogeneity between Gram-negative and Gram-positive sepsis, which was confirmed in the validation dataset.共有40个共同的差异基因集识别. The findings offer new insights into investigating the pathophysiology of bacterial sepsis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Organic
Tianwen Chen, Liyuan Wang, Shayu Li, Lichun Dong, Luxi Tan
Summary: In this study, a novel N-substituted diketopyrrolopyrrole (DPP) derivative, Ph-DPP, was synthesized, and it exhibited interaction with Lewis alkaline anions, such as F-. Both the typical electron-transfer-dominated anion-π interaction product, Ph-DPP-, and an unexpected isomer product, i-Ph-DPP, were observed. The formation mechanism of these products was investigated using density functional theory calculations, which suggested that a deprotonation initiation route is favored, providing valuable insight into the debated role of F(-) in non-covalent intermolecular interactions.
Article
Electrochemistry
Daobo Li, Xiaodan Zheng, Yonggang Yang, Guannan Kong, Meiying Xu
Summary: This study discovered that the Gram-positive strain Lysinibacillus varians GY32 expresses high levels of c-Cyts, expanding the potential of developing bioelectrochemical applications using diverse microbial models.
Article
Engineering, Environmental
Xiaoyan Qi, Huimin Wang, Xinyu Gao, Lei Zhang, Sen Wang, Xia Wang, Ping Xu
Summary: This study discovered an anaerobic bacterium, Pseudomonas putida B6-2, capable of degrading aromatic compounds and generating electricity in microbial fuel cells (MFCs). The bacterium successfully removed multiple aromatic compound pollutants and achieved high power densities in MFCs using a nickel-coated sponge with a three-dimensional macroporous structure as the anode. Preliminary analysis suggests that P. putida B6-2 may transfer extracellular electrons through redox mediators and microbial nanowires.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Agricultural Engineering
Peng Cheng, Yingchuan Zhang, Nianfang Ma, Lining Wang, Liqun Jiang, Zhen Fang, Yitong Wang, Xiangping Tan
Summary: Microbial fuel cell (MFC) has great potential in wastewater treatment and energy generation. This study reveals the electrochemical mechanism of extracellular anaerobic respiration in MFC inoculated with gram-positive Rhodococcus pyridinivorans HR-1. The MFC exhibits rapid recovery of currents on the anode, with redox peaks near -0.38 and -0.18 V. Blocking the biofilm-based pathway with a filter membrane did not prevent electricity generation in the HR-1 inoculated MFC, suggesting the presence of a self-secreted electron shuttle pathway.
BIORESOURCE TECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Rui Tao, Xianlin Qu, Zegao Wang, Fang Li, Lei Yang, Jiheng Li, Dan Wang, Kun Zheng, Mingdong Dong
Summary: This study demonstrates the tunability of photoresponse and excellent photodetection capability by constructing a MoS2 homojunction. Nb doping induces p-type doping in MoS2, significantly enhancing the photoresponse and detectivity. The research not only reveals the tunable photoexcitation mechanism but also provides a strategy for achieving high-performance photodetectors.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Physical
Zibo Li, Qian Liu, Qiang Li, Mingdong Dong
Summary: This study investigates the lubrication effect of hydrated halogen anions on positively charged surfaces using atomic force microscopy and friction force microscopy. The results show that the mechanical properties of the hydration layers depend on the concentration of electrolyte solutions, and the friction coefficient and friction dissipation are significantly lower at low concentrations. Superlubricity can be achieved in low concentration electrolyte solution. These findings provide insights into the origins of hydration lubrication.
Article
Chemistry, Multidisciplinary
Ziwei Su, Xiangxiang Zhang, Weichen Wang, Mingdong Dong, Xiaojun Han
Summary: This study demonstrates an efficient way to build self-powered artificial cells containing thylakoids that convert light energy into chemical energy to supply ATP. The generated ATP and NADPH can be used in promoting biological enzyme cascade reactions and provide a pathway for building more complex artificial cells.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Analytical
Fuxing Kang, Wei Yang, Mengwen Guo, Lars Borregaard Pedersen, Lars Peter Nielsen
Summary: Developing a PH3 microsensor with high selectivity and sensitivity is crucial for monitoring PH3 in aquatic or sedimentary environments. The sensor utilizes an Au-coated Pt working electrode and Pt guard electrode to selectively oxidize PH3, generating a current proportional to its concentration. By eliminating major H2S disruptors, the effects of other gases on the microsensor can be ignored.
ANALYTICAL CHEMISTRY
(2023)
Article
Thermodynamics
Botao Zhang, Shengjie Gong, Shichang Dong, Zhenqin Xiong, Qiang Guo
Summary: Boiling-induced vibration characteristics and mechanisms were investigated in pool boiling experiments. Turbulence and bubble collapse were identified as the two main mechanisms causing vibration. The peak amplitude of the first natural frequency increased with increasing heat flux and decreasing subcooling degree for turbulence-induced vibration. The peak amplitude of the second natural frequency increased with increasing heat flux for a certain subcooled temperature above 10 degrees C, and remained stable for lower subcooled temperatures. The dominant mechanism transitioned from bubble collapse to turbulence with decreasing subcooled temperature for a certain heat flux. The highest root-mean-square (RMS) value of vibration velocity corresponded to an increasing subcooled temperature with increasing heat flux.
APPLIED THERMAL ENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Deliang Zhang, Mingzheng Huang, Lasse Hyldgaard Klausen, Qiang Li, Suzhi Li, Mingdong Dong
Summary: Tribological properties are greatly influenced by environmental conditions, and the effect of liquid on friction is not well understood. In this study, the nanoscale friction of molybdenum disulfide (MoS2) in polar and nonpolar liquids was explored using friction force microscopy. The friction force exhibited layer-dependent behavior in liquids, with thinner samples having larger friction force. Interestingly, the polarity of the liquid significantly affected the friction, with polar water showing higher friction than nonpolar dodecane. Atomically resolved friction images and simulations revealed that liquid polarity influenced the friction behavior through liquid molecule arrangement and hydrogen-bond formation. This study provides insights into friction on two-dimensional materials in liquids and has promising applications in low-friction technologies.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Environmental Sciences
Nian Liu, Zhen Sun, Huan Zhang, Lasse Hyldgaard Klausen, Ryu Moonhee, Shifei Kang
Summary: Bacterial and photocatalysis techniques are widely used for the remediation of ammonia nitrogen wastewater. While traditional microbial methods are proven useful, there is a need for more efficient and controllable treatment methods to address the diverse cases of ammonia nitrogen pollution. Bacterial treatment relies on ammonia nitrogen oxidation-reduction by nitrifying and denitrifying bacteria, but these reactions are slow and uncontrolled. Photocatalysis, on the other hand, has advantages such as low temperature reaction and long life, but cannot perform multiple complex biochemical reactions. This review highlights recent achievements, key problems, and future directions for remediation of high-ammonia-nitrogen wastewater using bacterial treatment and photocatalysis techniques, including the potential of combining bacterial-photocatalysis techniques.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Jesper J. Bjerg, Jamie J. M. Lustermans, Ian P. G. Marshall, Anna J. Mueller, Signe Brokjaer, Casper A. Thorup, Paula Tataru, Markus Schmid, Michael Wagner, Lars Peter Nielsen, Andreas Schramm
Summary: Cable bacteria are filamentous microbes that conduct electrons via internal wires and couple sulfide oxidation between sediment layers. Other bacteria swarm around the anoxic part of oxygen-respiring cable bacteria and seem to transfer electrons to cable bacteria via soluble metabolites. This association and interaction with diverse partners may explain how oxygen from cable bacteria affects microbial communities and processes in anoxic environments.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Jamie J. M. Lustermans, Jesper J. J. Bjerg, Laurine D. W. Burdorf, Lars Peter Nielsen, Andreas Schramm, Ian P. G. Marshall
Summary: Cable bacteria are filamentous bacteria that play a major role in sediment geochemistry and microbial communities. Our study investigated the timing and drivers of flocking in a culture of freshwater cable bacteria. We found that flocking always occurred, regardless of the overall abundance or activity of the cable bacteria. Flocking was linked to the individual cable bacteria's electron conducting activity.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Bjorn Joos, Ken Elen, Jonathan van den Ham, Nicole Meulendijks, Pascal Buskens, Andreas Paulus, Koen Wouters, Jean Manca, Jan D'Haen, Sudhanshu Shukla, Bart Vermang, Marlies Van Bael, An Hardy
Summary: A benign aqueous route for bismuth-containing photoelectrodes using CuBi2O4 as a photocathode is proposed. The method offers advantages of elemental homogeneity, ease of fabrication, and production of phase-pure kusachiite crystalline phase. The fabricated CuBi2O4 photocathodes demonstrate competitive photocurrent densities, comparable to non-aqueous organic synthesis routes.
ADVANCED SUSTAINABLE SYSTEMS
(2023)
Article
Nanoscience & Nanotechnology
Rongrong Wu, Zengkai Wang, Zili Jia, Chenglong Li, Jie Wang, Lei Liu, Mingdong Dong
Summary: This study investigates the cross-fibrillation of A beta peptides and different hIAPP structures. The experimental results show that hIAPP aggregates enhance the fibrillation of A beta, especially hIAPP fibrils. Using quantitative nanomechanical mapping and molecular dynamic simulations, different hybrid amyloid strains can be distinguished based on their mechanical properties and structural transformations.
Article
Multidisciplinary Sciences
Steven J. Roeters, Kris Strunge, Kasper B. Pedersen, Thaddeus W. Golbek, Mikkel Bregnhoj, Yuge Zhang, Yin Wang, Mingdong Dong, Janni Nielsen, Daniel E. Otzen, Birgit Schiott, Tobias Weidner
Summary: This study reports a mechanism explaining why lipid membranes catalyze the formation of harmful aggregates at elevated concentrations.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Biomaterials
Chih-Hao Lu, Ching-Ting Tsai, Taylor Jones, Vincent Chim, Lasse H. H. Klausen, Wei Zhang, Xiao Li, Zeinab Jahed, Bianxiao Cui
Summary: This study develops a nanostructure-based curvature sensing platform that enables quantitative and multiplex analysis of curvature-sensitive proteins. It finds that the platform can be used to study both negative and positive curvature-sensing proteins. The NanoCurvS platform provides a robust, multiplex, and easy-to-use tool for quantitative analysis of both positive and negative curvature-sensing proteins.
BIOMATERIALS SCIENCE
(2023)
Article
Chemistry, Analytical
Fuxing Kang, Wei Yang, Mengwen Guo, Lars Borregaard Pedersen, Lars Peter Nielsen
Summary: Monitoring PH3 flux at the Earth's surface-atmosphere interface is a challenging task. A new amperometric PH3 microsensor with high selectivity and sensitivity is developed for in situ detection in aquatic or sedimentary environments. The sensor uses a Au-coated Pt working electrode and a Pt guard electrode, and employs a two-layer silicone membrane to selectively oxidize PH3 into H3PO4, producing a current proportional to the PH3 concentration (>2 nmol center dot L-1). The sensor is designed to eliminate interferences from H2S and other gases, making it suitable for environmental monitoring. A sedimentary profile example demonstrates the presence of high PH3 accumulations 13 mm below the sediment surface.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Jeanine S. Geelhoed, Casper A. Thorup, Jesper J. Bjerg, Lars Schreiber, Lars Peter Nielsen, Andreas Schramm, Filip J. R. Meysman, Ian P. G. Marshall
Summary: In this study, we identified a novel species of marine cable bacteria with a large diameter, named Candidatus Electrothrix gigas. The genomes of this species contain a gene encoding a novel actin-like protein (Bbp) that is also found in other giant bacteria. This suggests that there may be a genetic basis for large cell size. The Bbp protein may have a structural role in the cell or potentially facilitate intracellular transport.
MICROBIOLOGY SPECTRUM
(2023)