Article
Virology
Moustafa Gibory, Tone Bruun, Elmira Flem, Jennifer Lynn Dembinski, Ildri Haltbakk, Ketil Stordal, Svein Arne Nordbo, Kirsti Jakobsen, Elisebet Haarr, Truls Michael Leegaard, Susanne Gjeruldsen Dudman
Summary: After the introduction of the rotavirus vaccine in Norway, there was no clear evidence of vaccine-induced virus strain replacement, with G1P[8] being the most common type both pre and post vaccine implementation. The study revealed that 21.4% of cases detected were vaccine strains, highlighting the importance of continuous genotype surveillance for assessing vaccine program effectiveness.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Infectious Diseases
Nosheen Basharat, Asma Sadiq, Muhammad Dawood, Shahid Ali, Alam Khan, Rooh Ullah, Hayat Khan, Aamir Aziz, Hamid Ali, Aamer Ali Shah, Ijaz Ali, Jadoon Khan
Summary: This study aimed to detect the prevalence of Rotavirus A (RVA) in children in Pakistan after the introduction of rotavirus vaccines. Results showed a high prevalence of RVA in children with acute gastroenteritis (AGE), with no significant differences in gender and age among RVA infections. The seasons of winter, spring, and fall/autumn were statistically significant for RVA prevalence. Recommendations include implementing rotavirus vaccines, providing adequate nutrition and clean water supply for infants, and continuous surveillance to calculate the disease burden caused by RVA.
BMC INFECTIOUS DISEASES
(2021)
Article
Agriculture, Dairy & Animal Science
Gazanfar Abass, Zunjar Baburao Dubal, Kaushal K. Rajak, Balasaheb M. Kale, Abhay Raorane, Nitin Dudhe, Bilal Ahmad Malla, Dhananjay Desai, Dharmendra K. Sinha, Obli R. Vinodh Kumar, Yashpal Singh Malik
Summary: This study investigated the genotypes of RVA in pig populations in western and northern India. The results showed the circulation of G4P[6] and G9P[13] RVA strains in pigs, indicating evidence of inter-species transmission with human RVA strains.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ozer Ates, Kadir Yesilbag
Summary: This study successfully isolated RVA G10P[11] in the cattle farm, and found that this genotype plays an important role in the etiology of diarrhea in newborn calves. It should be considered in the selection of vaccines applied to the dams.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Public, Environmental & Occupational Health
Shoko Okitsu, Pattara Khamrin, Toshiyuki Hikita, Aksara Thongprachum, Ngan Thi Kim Pham, Sheikh Ariful Hoque, Satoshi Hayakawa, Niwat Maneekarn, Hiroshi Ushijima
Summary: The study in Japan showed that with the increase in rotavirus vaccination rates, the detection rates of RVA have decreased, and the genotype combinations have changed in different years.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2022)
Article
Agriculture, Dairy & Animal Science
Bilal Ahmad Malla, Zunjar Baburao Dubal, Anukampa Kadwalia, Gazanfar Abass, Obli Rajendran Vinodh Kumar, Ashok Kumar, Kaushal Kishore Rajak, Ishfaq Maqbool, Aquil Mohmad, Vivekanandhan Rangaraju, Arfa Fayaz
Summary: Rapid and reliable diagnosis of diarrhoeal disease is important for distinguishing the etiological agents and choosing suitable treatment. This study reveals a seasonal pattern in the occurrence of rotavirus in diarrheic children, calves, and piglets from Bareilly, Uttar Pradesh, India. The positivity rate is higher in winter compared to autumn.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Abderrahim Hatib, Rihabe Boussettine, Najwa Hassou, Abdelouaheb Benani, Moulay Mustapha Ennaji
Summary: This study aimed to monitor and evaluate the prevalence of Group A rotavirus (RVA) in oysters collected from Oualidia Lagoon, Morocco, and its relationship with watershed contribution. The results showed that 46.15% of the tested samples were positive for RVA, with statistically significant seasonal distribution. Therefore, consumption of RVA-contaminated oysters poses a serious threat to human health.
CELLULAR AND MOLECULAR BIOLOGY
(2022)
Article
Food Science & Technology
Chenang Lyu, Feifeng Lu, Zhentao Shi, Dapeng Wang
Summary: The study developed an ISC-RT-qPCR assay for detecting encapsulated RVA in oysters with higher sensitivity and accuracy than RT-qPCR. When applied to oysters collected from four different farms, ISC-RT-qPCR showed a higher detection rate of RVA compared to RT-qPCR, indicating its potential as a better method for disease monitoring and control.
Article
Food Science & Technology
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irene Iugovaz, Neda Nasheri
Summary: A method for extracting viral RNA from raw oysters has been developed, showing high efficiency in removing RT-PCR inhibitors and reliably detecting viral contamination. The method outperformed the ISO 15216-1 method and showed comparable efficiency to Method E, with fewer steps and shorter hands-on time.
Article
Biochemical Research Methods
Soontag Jung, Daseul Yeo, Zhaoqi Wang, Seoyoung Woo, Yeeun Seo, Md. Iqbal Hossain, Min Suk Rhee, Changsun Choi
Summary: This study successfully improved the primer set for the detection of SaV in food samples with high specificity and sensitivity, enabling efficient and reliable detection of SaV.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Engineering, Environmental
Daniel A. Okaali, Carolien Kroeze, Gertjan Medema, Peter Burek, Heather Murphy, Innocent K. Tumwebaze, Joan B. Rose, Matthew E. Verbyla, Sowed Sewagudde, Nynke Hofstra
Summary: This study investigates the impact of fecal pathogens on surface water sanitation, considering factors such as future population growth, urbanization, and climate change, providing a model for simulating rotavirus river inputs and concentrations in Uganda. The results indicate that urban sanitation conditions are a key factor influencing rotavirus concentrations.
Article
Veterinary Sciences
Chun Qi Li, Li Qun Hu, Guo Ping Liu, Yan Wang, Tong Li, Shao Xian Chen, Xiao Lin Yang, Li Xin Ma, Jian Guo Zeng
Summary: The study established a convenient, reliable, specific, sensitive, and anti-interference detection method for PEDV and PDCoV using duplex nested RT-PCR. This method can also determine the molecular epidemiological background.
BMC VETERINARY RESEARCH
(2023)
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Chemistry, Multidisciplinary
Xander F. van Kooten, Yana Rozevsky, Yulia Marom, Efrat Ben Sadeh, Amit Meller
Summary: Most RNA-based COVID-19 diagnostic methods use enzymatic amplification for high sensitivity but rely on arbitrary thresholding. This study introduces a solid-state nanopore sensing technology for label-free quantification of SARS-CoV-2 RNA in clinical nasal swab samples. The method allows for ratiometric quantification of RNA abundance through single-molecule counting and length-based nanopore identification of dsDNA. The results show that this approach can provide quantitative positive outcomes in clinical samples classified as negative by RT-qPCR.
Article
Environmental Sciences
Enric Cuevas-Ferrando, Alba Perez-Cataluna, Ana Allende, Susana Guix, Walter Randazzo, Gloria Sanchez
Summary: The study evaluated the analytical performance of dead-end hollow fiber ultrafiltration combined with different secondary concentration options in monitoring SARS-CoV-2 viral particles, finding that PEG-precipitation and centrifugal filtration are effective methods for virus recovery.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Xiaoxue Xie, Zhenqi Peng, Ziqi Wang, Xinyi Hua, Zhifang Wang, Keqin Deng, Xiumei Yang, Haowen Huang, Xuanyong Liu
Article
Biochemistry & Molecular Biology
Baoting Sun, Zichen Wang, Xiaoyi Wang, Mengxia Qiu, Zhijin Zhang, Ziyuan Wang, Jiandong Cui, Shiru Jia
Summary: Phenylketonuria (PKU) is a genetic defect in phenylalanine metabolism. A novel paper-based biosensor for semi-quantitative detection of phenylalanine in urine samples was developed, showing good stability and reusability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Analytical
Xinyi Hua, Zhifang Wang, Ziqi Wang, Linlin Chen, Zaichun Zhou, Junlin Ouyang, Keqin Deng, Xiumei Yang, Haowen Huang
Summary: An innovative biosensing assay was developed for simplified, cost-effective, and sensitive detection. By treating target proteins with iron porphyrin in situ, a stable protein complex with peroxidase activity was formed, providing visible signals comparable to ELISA. This immunoassay eliminates the need for expensive cross-linking processes, making it practical for clinical diagnosis, food safety, and environmental monitoring.
ANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Ziyuan Wang, Fengzhu Guo, Tianyu Dong, Zhilei Tan, Mohamed Abdelraof, Zichen Wang, Jiandong Cui, Shiru Jia
Summary: The metabolomic analysis revealed significant differences in the biosynthesis of epsilon-PL between the high-yield strain 6#-7 and the original strain TUST. Improved glucose transport and absorption, enhanced enzymatic activities related to epsilon-PL synthesis, and increased resistance to epsilon-PL were found in 6#-7. These findings suggest an enhanced ability of mutagenized strain 6#-7 to synthesize epsilon-PL compared to the original strain.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Immunology
Wang Wang, Wei-Zhen Liu, Zi-Liang Wang, Dong-Xiao Duan, Xue-Yun Wang, Shi-Jin Liu, Zhi-Ju Wang, Guo-Gang Xing, Ying Xing
Summary: This study used standard and modified social defeat stress mouse models to investigate the impact of perioperative social defeat stress on postsurgical pain in male and female mice. The results showed that social defeat stress could induce prolonged postsurgical pain, and spinal microglial activation plays a key role in this process in male mice. However, this effect was not significant in female mice.
BRAIN BEHAVIOR AND IMMUNITY
(2022)
Review
Pediatrics
Zijun Wang, Siya Zhao, Yuyi Tang, Zhili Wang, Qianling Shi, Xiangyang Dang, Lidan Gan, Shuai Peng, Weiguo Li, Qi Zhou, Qinyuan Li, Joy James Mafiana, Rafael Gonzalez Cortes, Zhengxiu Luo, Enmei Liu, Yaolong Chen
Summary: This systematic review evaluated the efficacy and safety of using potential drugs, remdesivir and glucocorticoid, in treating children and adolescents with COVID-19, as well as using intravenous immunoglobulin (IVIG) in treating MIS-C. The included studies showed that remdesivir may cause adverse events and death, while glucocorticoids were associated with mortality and mechanical ventilation rate. IVIG combined with methylprednisolone had better clinical efficacy for MIS-C patients with severe symptoms. The current evidence is of low quality, and high-quality randomized controlled trials are recommended to provide substantial evidence for guidelines development.
EUROPEAN JOURNAL OF PEDIATRICS
(2022)
Article
Environmental Sciences
Yiteng Zhang, Xingxing Cheng, Ziliang Wang, Mudassir Hussain Tahir, Zhiqiang Wang, Xuetao Wang, Chao Wang
Summary: This study proposed a novel multi-stage utilization process for cabbage waste, which involved low-temperature extraction, medium-temperature thermolysis, and high-temperature activation. The process yielded valuable products such as plant extracts, pyrolytic oil, and biochar, with optimized temperatures and performance characteristics.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Siyu Song, Jing Wang, Guanwen Liu, Lu Ding, Yaxin Li, Hongyu Qi, Lai Wei, Jiachao Zhao, Tian Chen, Meiru Zhao, Ziyuan Wang, Yingying Yang, Daqing Zhao, Xiangyan Li, Zeyu Wang
Summary: In this study, the mechanism of the Wenfei Buqi Tongluo (WBT) formula in inhibiting inflammatory response during idiopathic pulmonary fibrosis (IPF) was explored. Network pharmacology and in vivo experiments were used to investigate the effects of the WBT formula. The results showed that the WBT formula can suppress IPF by inhibiting inflammation via the TLR4/MyD88/NF-kappa B pathway.
BIOMED RESEARCH INTERNATIONAL
(2022)
Article
Pharmacology & Pharmacy
Yingying Yang, Lu Ding, Tingting Bao, Yaxin Li, Jing Ma, Qingwei Li, Zezheng Gao, Siyu Song, Jing Wang, Jiachao Zhao, Ziyuan Wang, Daqing Zhao, Xiangyan Li, Zeyu Wang, Linhua Zhao, Xiaolin Tong
Summary: Research has shown that Qimai Feiluoping decoction (QM) can improve PF symptoms in COVID-19 convalescent patients by regulating epithelial-mesenchymal transition, extracellular matrix degradation, and the TGF-beta signaling pathway. QM contains multiple active components and exerts its anti-PF effects by inhibiting the TGF-beta/Smad3 pathway.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Lu Ding, Yaxin Li, Yingying Yang, Siyu Song, Hongyu Qi, Jing Wang, Ziyuan Wang, Jiachao Zhao, Wei Zhang, Linhua Zhao, Daqing Zhao, Xiangyan Li, Zeyu Wang
Summary: This study analyzed the chemical components of the WBT formula and predicted its potential targets and mechanism against PF using network pharmacology. It was found that WBT can block the progression of PF by inhibiting EMT and promoting ECM degradation via the TGF-beta/Smad3 pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Food Science & Technology
Lei Zhang, Ziyuan Wang, Yi Jiao, Zichen Wang, Xiuming Tang, Zhanxin Du, Zeyang Zhang, Shihai Lu, Changsheng Qiao, Jiandong Cui
Summary: Biobased polymer packaging films have witnessed significant developments in recent years. In this study, biodegradable packaging films with antimicrobial properties and mechanical strength were successfully developed. The films showed improved UV/visible light barrier properties and enhanced antimicrobial activities, making them promising for food packaging and preservation systems.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Applied
Zi-Xuan Wu, De-Yang Li, Miao Shen, Zi-Ye Wang, Zi-Wei Wang, Yu-Xin Liu, Yan-Hong Bai, Da-Yong Zhou
Summary: The appropriate sous-vide cooking conditions were determined using differential scanning calorimetry analysis. Sous-vide cooking significantly improved the texture indicators of scallop adductor muscle (SAM) and altered the protein structure and chemical interactions, resulting in changes in the microstructure and water distribution of muscle fibers.
Article
Plant Sciences
Qian Wang, Er-Ling Ou, Pu-Chang Wang, Ying Chen, Zi-Yuan Wang, Zhi-Wei Wang, Xiang-Wen Fang, Jin-Lin Zhang
Summary: This study provides new insights into the role of the plant growth-promoting rhizobacterium B. amyloliquefaciens GB03 as a mediator of defense against nitrogen deficiency in non-leguminous grass tall fescue. The results show that GB03 inoculation can significantly enhance the growth and development of tall fescue, particularly under total nitrogen conditions.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Psychiatry
Yuanyuan Gui, Xiaocheng Zhou, Zixin Wang, Yiliang Zhang, Zhaobin Wang, Geyu Zhou, Yize Zhao, Manhua Liu, Hui Lu, Hongyu Zhao
Summary: This study examined the associations between the predicted polygenic risk scores of six psychiatric disorders and cognitive functions, behavior, and brain imaging traits using publicly available genome-wide association studies and individual-level data from the Adolescent Brain Cognitive Development study and the UK Biobank study. Significant heterogeneity was found in genetic associations between cognitive traits and psychiatric disorders, as well as behavior and brain imaging, between sexes.
TRANSLATIONAL PSYCHIATRY
(2022)
Article
Public, Environmental & Occupational Health
Zhenkun Wang, Aihua Du, Hong Liu, Ziwei Wang, Jifa Hu
Summary: This study investigates the temporal and regional trends, as well as risk factors, of cardiovascular diseases (CVDs) worldwide from 1990 to 2017. The study finds that the number of deaths from CVDs has increased over time, but the age-standardized mortality rate has decreased. Metabolic risks and behavioral factors are identified as the main contributors to CVDs.
JOURNAL OF EPIDEMIOLOGY AND GLOBAL HEALTH
(2022)
