Article
Infectious Diseases
Nicole de Mello Fiallos, Ana Luiza Ribeiro Aguiar, Bruno Nascimento da Silva, Mariana Lara Mendes Pergentino, Marcos Fabio Gadelha Rocha, Jose Julio Costa Sidrim, Debora Castelo Branco de Souza Collares Maia, Rossana de Aguiar Cordeiro
Summary: This study aimed to investigate the antimicrobial potential of promethazine (PMZ) and chlorpromazine (CPZ) against dual-species biofilms of Enterococcus faecalis and Candida albicans. Results showed that PMZ was the most efficient phenothiazine, capable of reducing cell counts, biomass, biovolume, carbohydrate and protein contents of the biofilms. PMZ and CPZ did not increase the antimicrobial activity of the irrigants.
Article
Microbiology
Qingsong Jiang, Qi Jing, Biao Ren, Lei Cheng, Xuedong Zhou, Wenli Lai, Jinzhi He, Mingyun Li
Summary: This study investigated the effect of Enterococcus faecalis on the biofilm formation, hyphal morphogenesis, and virulence gene expression of Candida albicans. The results showed that exposure to E. faecalis culture supernatant promoted the biofilm formation and hyphal morphogenesis of C. albicans, increased the production of exopolymeric substances, cellular surface hydrophobicity, adhesion force of biofilms, and the expression level of EFG1 virulence gene.
Article
Biotechnology & Applied Microbiology
Nicole de Mello Fiallos, Ana Luiza Ribeiro Aguiar, Bruno Nascimento da Silva, Marcos Fabio Gadelha Rocha, Jose Julio Costa Sidrim, Debora Castelo Branco de Souza Collares Maia, Rossana de Aguiar Cordeiro
Summary: This study optimized an in vitro dual-species biofilm model of Enterococcus faecalis and Candida albicans for endodontic research. The established model can be used to explore interkingdom relationships and develop new strategies against periapical infections.
Article
Microbiology
Yuting Fu, Wenjing Wang, Qiao Zeng, Ting Wang, Weidong Qian
Summary: The study demonstrated the efficacy of luteolin against planktonic cell growth, adhesion, and biofilm formation of Candida albicans and Enterococcus faecalis, suggesting its potential as a promising antibiofilm agent for the therapeutic management of biofilm-related infections induced by these two species.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Physics, Applied
Yinghui Wen, Yun Luo, Xiaoling Wei, Haibo Tan, Rui Ai, Zhujiang Xiong, Liming Ye
Summary: This study investigates the antibacterial effects of liquid discharge cold plasma (LDCP) on Enterococcus faecalis (E. faecalis) planktonic cultures and root canal biofilms. The results show that LDCP treatment has a significant antibacterial effect on both planktonic E. faecalis and biofilms, surpassing the efficacy of 0.2% chlorhexidine treatment. This work suggests the potential application of liquid discharge plasma devices in root canal treatment.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Abhishek Parolia, Haresh Kumar, Srinivasan Ramamurthy, Thiagarajan Madheswaran, Fabian Davamani, Malikarjuna Rao Pichika, Kit-Kay Mak, Amr S. Fawzy, Umer Daood, Allan Pau
Summary: The study focused on the antibacterial effect of propolis nanoparticles as an endodontic irrigant against Enterococcus faecalis biofilm and compared it with other irrigants, revealing that PN300 was significantly more effective in reducing CFUs compared to other groups. In addition, PN300 showed similar antibacterial efficacy to 6% NaOCl and 2% CHX at different time intervals and depths.
Article
Biology
Yuliya Morozova, Iva Voborna, Radovan Zizka, Katerina Bogdanova, Renata Vecerova, Dominik Rejman, Milan Kolar, Duy Dinh Do Pham, Pavel Holik, Roman Mostek, Matej Rosa, Lenka Pospisilova
Summary: The study focused on the ex vivo effects of second- and third-generation LPPOs on Enterococcus faecalis in root canal samples, comparing them with other disinfectants. Results showed that LPPOs demonstrated superior bactericidal effects compared to the tested disinfectants by reducing planktonic bacterial cells and inhibiting biofilm formation.
Article
Pharmacology & Pharmacy
Virginia Barreto Lordello, Andreia Bagliotti Meneguin, Sarah Raquel de Annunzio, Maria Pia Taranto, Marlus Chorilli, Carla Raquel Fontana, Daniela Cardoso Umbelino Cavallini
Summary: The orodispersible film developed in this study successfully delivered probiotic Enterococcus faecium CRL 183 into the oral cavity, demonstrating good antifungal activity against Candida albicans and reducing biofilm formation.
Article
Dentistry, Oral Surgery & Medicine
Pasika Thienngern, Anchana Panichuttra, Chootima Ratisoontorn, Chuanchom Aumnate, Oranart Matangkasombut
Summary: In an in vitro root canal model, chitosan paste was found to be more effective than Ca(OH)(2) in reducing viable E. faecalis biofilm and significantly reducing viable bacteria. However, it showed no significant difference in efficacy against C. albicans compared to Ca(OH)(2).
Article
Infectious Diseases
Monica Naufel Sousa, Alessandra Teixeira Macedo, Gabriella Freitas Ferreira, Haryne Lizandrey Azevedo Furtado, Aruana Joaquim Matheus Costa Rodrigues Pinheiro, Lidio Goncalves Lima-Neto, Valeria Costa Fontes, Rayana Larissa Pinheiro Soares Ferreira, Cristina Andrade Monteiro, Angela Falcai, Lillian Nunes Gomes, Queila da Silva Rosa Braganca, Dennyse de Sousa Brandao Torres, Livia Camara de Carvalho Galvao, Rodrigo Assuncao Holanda, Julliana Ribeiro Alves Santos
Summary: Failure in endodontic treatments is mainly due to the difficulty in eradicating microbes in the root canal system. Punica granatum leaf extract shows potential as an alternative canal dressing due to its antimicrobial properties. This study evaluated the antimicrobial activity of the leaf extract alone or in combination with calcium hydroxide against Enterococcus faecalis and Candida albicans.
Article
Chemistry, Physical
Stefan Kranz, Andre Guellmar, Franziska Braeutigam, Silke Tonndorf-Martini, Markus Heyder, Markus Reise, Bernd Sigusch
Summary: The study showed that chloramine-T is highly effective in suppressing Enterococcus faecalis inside dentinal tubules, while calcium hydroxide and sodium hypochlorite have limited effects in comparison.
Article
Medicine, General & Internal
Magdalena Moryl, Aleksandra Palatynska-Ulatowska, Agnieszka Maszewska, Iwona Grzejdziak, Silvia Dias de Oliveira, Marieli Chitolina Pradebon, Liviu Steier, Antoni Rozalski, Jose Antonio Poli de Figueiredo
Summary: This study isolated and characterized two phages against E. faecalis and confirmed their lytic activity against the bacteria. The phages showed efficacy against E. faecalis biofilm in an ex vivo model, supporting the potential of phage therapy for prevention and treatment of E. faecalis-associated infections.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Microbiology
Xinyi Kuang, Jin Zhang, Xian Peng, Qian Xie, Jiyao Li, Xuedong Zhou, Youfu Luo, Xin Xu
Summary: The study demonstrates that II-6s exhibits potent antimicrobial activity against Enterococcus faecalis without inducing drug resistance, and has moderate cytotoxicity. Moreover, II-6s shows significant bactericidal effect against E. faecalis in dentinal tubules.
JOURNAL OF ORAL MICROBIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Shymaa Shaaban, Salma Genena, Alaaeldin Elraggal, Gamal M. Hamad, Marwa A. Meheissen, Sybel Moussa
Summary: This study evaluated the antibacterial activity of multi-strain probiotics supernatants (MSP) - Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus acidophilus as an intracanal medication on Enterococcus faecalis (E. faecalis) biofilm in a tooth model. The results showed that MSP has a similar antibacterial effect to calcium hydroxide (Ca(OH)(2)).
Article
Biotechnology & Applied Microbiology
Qian Du, Shasha Yuan, Shuangyuan Zhao, Di Fu, Yifei Chen, Yuan Zhou, Yangpei Cao, Yuan Gao, Xin Xu, Xuedong Zhou, Jinzhi He
Summary: The co-infection of Candida albicans and Enterococcus faecalis was found to exacerbate periapical lesions by enhancing biofilm virulence. Co-inoculation also led to increased osteoclastic bone resorption and suppressed osteoblastic bone formation. The synergistic effects up-regulated inflammatory cytokines.
Article
Dentistry, Oral Surgery & Medicine
Anja Ivica, Shengjile Deari, Raphael Patcas, Franz E. Weber, Matthias Zehnder
JOURNAL OF ENDODONTICS
(2020)
Article
Medicine, General & Internal
Anja Ivica, Chafik Ghayor, Matthias Zehnder, Silvio Valdec, Franz E. Weber
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Dentistry, Oral Surgery & Medicine
S. Deari, M. Zehnder, A. Al-Jadaa
INTERNATIONAL ENDODONTIC JOURNAL
(2020)
Review
Medicine, General & Internal
Amina Munir, Matthias Zehnder, Dan-Krister Rechenberg
JOURNAL OF CLINICAL MEDICINE
(2020)
Editorial Material
Medicine, General & Internal
Matthias Zehnder
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Medicine, General & Internal
Nidambur Vasudev Ballal, Henry F. Duncan, Namith Rai, Prateek Jalan, Matthias Zehnder
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Dentistry, Oral Surgery & Medicine
D. K. Rechenberg, A. Munir, M. Zehnder
Summary: The study aimed to evaluate and correlate three different states of severely painful endodontically derived inflammation with their depiction on periapical radiographs using periapical index (PAI) scores. The results showed a good correlation between the clinically diagnosed main location of periapical inflammation and PAI scores, although in some cases, the PAI scores underestimated the clinical situation.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Article
Dentistry, Oral Surgery & Medicine
Erik Trautmann, Thomas Attin, Dirk Mohn, Matthias Zehnder
Summary: Strong oxidizing agents like sodium hypochlorite and hydrogen peroxide are commonly used as root canal irrigants in dentistry. While both are bleaching agents, they differ in stability and pH levels in aqueous solutions. Sodium hypochlorite is more stable at high pH levels, while hydrogen peroxide gains bleaching power on organic stains when alkalized.
JOURNAL OF ENDODONTICS
(2021)
Article
Chemistry, Physical
Nidambur Vasudev Ballal, Anja Ivica, Pamela Meneses, Raj Kumar Narkedamalli, Thomas Attin, Matthias Zehnder
Summary: Adding HEDP to NaOCl solution can create a combined endodontic irrigant with soft tissue-dissolving and decalcifying effects. Experiments showed a significant synergistic effect between NaOCl and ultrasonic activation in tissue weight loss and gelatin dissolution, without negative impact from HEDP.
Review
Dentistry, Oral Surgery & Medicine
Matthias Zehnder, Georgios N. Belibasakis
Summary: This narrative review discusses the use of biomarkers in clinical Endodontic research, highlighting the importance of addressing clinical problems through suitable diagnostic biomarkers. The study focuses on two clinical issues, reversible versus irreversible pulpitis and persistent idiopathic dentoalveolar pain, to demonstrate the role of biomarkers in understanding complex dental pathologies and guiding appropriate treatment strategies.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Frank Sanner, David Sonntag, Norbert Hambrock, Matthias Zehnder
Summary: Persistent idiopathic dentoalveolar pain (PIDAP) mainly affects females and is typically associated with undisturbed sleep and periodontal allodynia.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
N. Ballal, H. F. Duncan, D. B. Wiedemeier, N. Rai, P. Jalan, V Bhat, V. S. Belle, M. Zehnder
Summary: In this study, it was found that the levels of neutrophil gelatinase 9 (MMP-9)/total protein (TP) and NaOCl lavage significantly influenced pulp survival in cariously exposed and directly capped teeth, while mild preoperative discomfort did not have an impact.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Polymer Science
Nidambur Vasudev Ballal, Amal Roy, Matthias Zehnder
Summary: This study evaluated the impact of root canal wall conditioning on the adhesion of different root canal sealers, finding that the use of NaOCl with HEDP was beneficial for both types of sealers. The adhesion of the epoxy resin sealer was negatively affected by NaOCl deproteinization in a dose-dependent manner, while the CaSi sealer showed the opposite effect.
Article
Dentistry, Oral Surgery & Medicine
Anas Al-Jadaa, Zehraa Saidi, Maise Mahmoud, Rahaf Al-Taweel, Matthias Zehnder
Summary: This study compared the in vitro efficacy and safety of different irrigant activation devices in apically closed and open root canal systems. The results showed that ultrasonic agitation was more effective in cleaning the central area of the root canal, while it had a lower effect in cleaning the simulated periapical lesion. Therefore, ultrasonic agitation was found to be more targeted and safer after the administration of sodium hypochlorite.
JOURNAL OF ENDODONTICS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Kevin Hofpeter, Matthias Zehnder, Michael Huelsmann, Anas Al-Jadaa, Shengjile Deari
Summary: This study aimed to develop a standardized curved root canal model in bovine dentine and compare its behavior to resin in standard plastic training blocks during chemo-mechanical preparation. The study found that dentine had higher microhardness compared to resin, and dentine exhibited greater canal transportation, which was slightly increased by EDTA. The results are important for testing and comparing materials and concepts of chemo-mechanical root canal instrumentation.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)