Article
Biochemistry & Molecular Biology
Vladyslav Bondarenko, Mikhail Nikolaev, Dimitri Kromm, Roman Belousov, Adrian Wolny, Marloes Blotenburg, Peter Zeller, Saba Rezakhani, Johannes Hugger, Virginie Uhlmann, Lars Hufnagel, Anna Kreshuk, Jan Ellenberg, Alexander van Oudenaarden, Anna Erzberger, Matthias P. Lutolf, Takashi Hiiragi
Summary: Using engineering methods, we recreated the implantation process of mouse embryos outside the uterus and revealed the crucial role of physical interactions between the embryo and uterus in embryo morphogenesis. The study found that adhesion between the trophoblast and the uterine matrix is necessary for the transition of the blastocyst to the egg cylinder.
Article
Microbiology
Li Wang, Junyu Chen, Lin He, Hanbo Liu, Yan Liu, Zonghui Luan, Hong Li, Weixin Liu, Mengjun Luo
Summary: The aim of this study was to investigate the distribution and differences in the uterus and vaginal microbiome between women with early embryonic arrest and those with normal pregnancy using high-throughput sequencing. The results showed significant differences in Lactobacillus, Bacteroidetes, and Helicobacter in the vagina between the two groups. The study also identified specific dominant bacterial species and genera in both the vagina and uterus.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Reproductive Biology
Kailiang Li, Quanxi Li, Shah Tauseef Bashir, Brent M. Bany, Romana A. Nowak
Summary: Conditional knockout of Basigin results in decreased fertility in mice, with abnormalities in implantation, decidualization, placentation, and parturition in the uteri of knockout mice. This suggests that Basigin plays a critical role in successful pregnancy through its functions in implantation and decidualization.
BIOLOGY OF REPRODUCTION
(2021)
Article
Veterinary Sciences
Katarzyna Buska-Mach, Anna Ewa Kedzierska, Adam Lepczynski, Agnieszka Herosimczyk, Malgorzata Ozgo, Pawel Karpinski, Agnieszka Gomulkiewicz, Daria Lorek, Anna Slawek, Piotr Dziegiel, Anna Chelmonska-Soyta
Summary: This study demonstrated that pregnant female mice can distinguish between embryos of normal and impaired biological quality before implantation, showing differential gene expression in signal transduction pathways and differential protein expression in splenic CD4(+) T lymphocytes.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Miguel Tavares Pereira, Renata Nowaczyk, Rita Payan-Carreira, Sonia Miranda, Selim Aslan, Duygu Kaya, Mariusz P. Kowalewski
Summary: The study found that the expression of certain immune factors in canine pregnancy is influenced by the presence of embryos, with varying levels of immune regulation and anti-inflammatory conditions at different stages of pregnancy.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Immunology
Jiri Fronek, Libor Janousek, Jakub Kristek, Jaroslav Chlupac, Marek Pluta, Robert Novotny, Jana Maluskova, Michael Olausson
Summary: This study reported the first live birth after a deceased donor uterus transplant in Europe, demonstrating that nulliparous uteri may be a suitable source of uterine grafts for transplantation. Stenting appears to be a feasible treatment for vaginal anastomotic stenosis.
Article
Obstetrics & Gynecology
Alexa Bishop, Judith E. Cartwright, Guy S. Whitley
Summary: Stanniocalcin-1 (STC-1) is a glycoprotein hormone with wide expression in female reproductive tissues, playing a vital role in processes such as ovarian follicular development, blastocyst implantation, and placental development. Dysregulation of STC-1 is associated with severe reproductive disorders, highlighting the need for further research on its regulation and function.
HUMAN REPRODUCTION UPDATE
(2021)
Article
Reproductive Biology
Victoria de Brun, Juan J. Loor, Hugo Naya, Andrea Grana-Baumgartner, Mario Vailati-Riboni, Omar Bulgari, Khuram Shahzad, Jose Alfonso Abecia, Cecilia Sosa, Ana Meikle
Summary: Transcriptomics and bioinformatics were used to study the impact of undernutrition and pregnancy on uterine immune system and remodeling. Results showed that pregnancy altered gene expression, particularly immune system and uterine remodeling genes, while undernutrition attenuated these effects.
Article
Biochemistry & Molecular Biology
Rui Cao, Zhen-Shan Yang, Sui-Li Hu, Shi-Jin Liang, Shu-Miao Zhang, Song-Qi Zhu, Lin Lu, Cheng-Hong Long, Si-Tong Yao, Yong-Jiang Ma, Xiao-Huan Liang
Summary: This study focused on the molecular mechanism of actin assembly regulation in uterine smooth muscle during early pregnancy. The results showed that Tagln plays a critical role in F-actin polymerization, and decreases in actin assembly are crucial for optimal embryo implantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Reproductive Biology
Jessica S. Dudley, Christopher R. Murphy, Michael B. Thompson, Bronwyn M. McAllan
Summary: This review discusses the morphological and molecular changes to the uterine epithelium preceding implantation among mammals, aiming to identify commonalities and constraints of these cellular changes for understanding the evolution of placentation in mammals and suggesting directions for future research. The complex modifications to the ultrastructure of uterine epithelial cells during the formation of placenta are compared and discussed, highlighting similarities in changes to the cytoskeleton and gross morphology of the cells in all eutherians and marsupials studied. Further research is needed to understand the evolution of placentation among viviparous mammals, particularly concerning the level of placental invasiveness, hormonal control, and genetic underpinnings of pregnancy in marsupial taxa.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Annika Geijer-Simpson, Haidee Tinning, Tiago H. C. De Bem, Ioannis Tsagakis, Alysha S. Taylor, Laura Hume, Lisa M. Collins, Niamh Forde
Summary: Environmental stressors experienced during fetal development can have long-lasting effects on the reproductive potential of offspring. This study used a porcine model to investigate the impact of prenatal steroid hormone exposure on the reproductive tracts of female offspring. Results showed that the sex ratio of the litter influenced the number of primordial follicles in the ovaries, with male-biased litters having higher numbers of follicles in low birth weight pigs. However, there was no effect of sex bias on endometrial gland development. Additionally, lower birth weight was associated with a decreased proportion of glands in the endometrium and increased variability in both male-biased and female-biased litters. The study highlights the importance of prenatal factors in shaping reproductive health in females.
BIOLOGY OF REPRODUCTION
(2023)
Article
Biology
Mitsunori Matsuo, Jia Yuan, Yeon Sun Kim, Amanda Dewar, Hidetoshi Fujita, Sudhansu K. Dey, Xiaofei Sun
Summary: This study reveals that mice with uterine depletion of Foxa2 may enter embryonic diapause, with Msx1 and LIF potentially playing a role in resuming pregnancy.
Article
Cell Biology
Shuo Wan, Yadong Sun, Jinbao Zong, Wanqing Meng, Jiacong Yan, Kexin Chen, Sanfeng Wang, Daji Guo, Zhiqiang Xiao, Qinghua Zhou, Zhinan Yin, Meixiang Yang
Summary: Infertility is a global reproductive health problem, and the role of m(6)A modification in infertility is still unknown. This study reveals that METTL3-dependent m(6)A methylation plays a crucial role in female fertility by balancing estrogen and progesterone signaling.
CELL DEATH & DISEASE
(2023)
Article
Environmental Sciences
Xiaoyun Wu, Xiaowen Yang, Yuchai Tian, Pengchong Xu, Huifeng Yue, Nan Sang
Summary: The study found that BPB and BPAF, analogs of BPA, have adverse effects on uterine health, causing endometrial contraction, decreased epithelial height, and increased gland numbers. Bioinformatics analysis revealed that both BPB and BPAF disrupt the immune landscape of the uterus. Furthermore, eight genes associated with BPB and BPAF exposure were found to be related to uterine corpus endometrial carcinoma (UCEC), and high expression of Srd5a1 was significantly correlated with poor prognosis in patients.
ENVIRONMENT INTERNATIONAL
(2023)
Review
Agriculture, Dairy & Animal Science
Anna J. Korzekwa, Angelika M. Kotlarczyk
Summary: The use of assisted reproduction techniques in Cervidae is crucial for conservation efforts, particularly in managing endangered species and farmed deer. While some techniques like IVF have shown satisfactory results, others such as cryopreservation still require refinement.
Article
Oncology
Yunxuan Wang, Ting Yan, Jialiang Li, Wenmeng Wang, Qiong Wu, Jinming Shi, Dangdang Li, Daniel B. Stovall, Qingyuan Zhang, Guangchao Sui
EXPERIMENTAL CELL RESEARCH
(2020)
Article
Pharmacology & Pharmacy
Shah Jehan, Chen Zhong, Guangyue Li, Syed Zulqarnain Bakhtiar, Dangdang Li, Guangchao Sui
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Aixin Hao, Yu Wang, Xiao Zhang, Jialiang Li, Yingzhou Li, Dangdang Li, George Kulik, Guangchao Sui
Summary: The study identified a lncRNA, HYOU1-AS, which promotes the progression of triple negative breast cancer (TNBC) by upregulating HYOU1. Inhibiting HYOU1-AS could suppress the proliferation and migration of TNBC cells, suggesting its potential as a therapeutic target. Increased levels of HYOU1 correlated with poor clinical outcomes in breast cancer patients, indicating the significance of the HYOU1-AS/HYOU1 axis in TNBC progression.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Oncology
Cheng Yi, Guangyue Li, Wenmeng Wang, Yixuan Sun, Yueling Zhang, Chen Zhong, Daniel B. Stovall, Dangdang Li, Jinming Shi, Guangchao Sui
Summary: The study revealed that synthetic peptides derived from YPB and OPB domains disrupted the interaction between YY1 and EZH2, inhibiting the proliferation of breast cancer cells and reducing tumor growth. Additionally, these peptides interfered with H3K27 trimethylation and primarily targeted the PTENP1 gene, highlighting its importance in the anticancer activity of YPB and OPB peptides.
Article
Biochemistry & Molecular Biology
Lanlan Zhang, Ting Yan, Wenmeng Wang, Qiong Wu, Guangyue Li, Dangdang Li, Daniel B. Stovall, Yunxuan Wang, Yuhua Li, Guangchao Sui
Summary: The study found that G-quadruplex structures formed in the AKT1 promoter and its 3'-UTR positively regulate AKT1 gene expression. SP1 can bind to its consensus sites, but the strongest promoter strength requires the presence of both G4 motifs and SP1 binding sites.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Shuang Qiu, Chen Zhong, Bo Zhao, Guangyue Li, Jinhua Wang, Shah Jehan, Jialiang Li, Xudong Zhao, Dangdang Li, Guangchao Sui
Summary: Ellagic acid induced G1 phase arrest and promoted apoptosis of HCC cells by activating the p21 gene and downregulating the MCM2-7 genes, respectively. The findings of this study provide valuable insights for the development of novel therapeutic strategies for HCC patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Jialiang Li, Guangyue Li, Yige Qi, Yao Lu, Hao Wang, Ke Shi, Dangdang Li, Jinming Shi, Daniel B. Stovall, Guangchao Sui
Summary: In this study, SRSF5 was identified as a key regulator promoting the splicing of DMTF1 beta and gamma, while reducing DMTF1 alpha splicing. The expression of SRSF5 was positively correlated with the DMTF1 beta/alpha ratio in breast cancer samples.
Review
Biochemistry & Molecular Biology
Lin Zhang, Shubo Wang, Wenmeng Wang, Jinming Shi, Daniel B. Stovall, Dangdang Li, Guangchao Sui
Summary: In live cells, proteins and nucleic acids can form isolated phases through multivalent interactions, a process known as liquid-liquid phase separation (LLPS). LLPS has been recognized as a general mechanism for the intracellular organization of biomolecules and regulates the assembly and composition of membraneless organelles and condensates. Aberrant LLPS can contribute to the onset and progression of diseases, including neurodegenerative disorders and cancers. This review summarizes the properties of different membraneless organelles and condensates, and discusses various phase separation-regulated biological processes, as well as how dysregulated LLPS may contribute to human diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Jinhua Wang, Dangdang Li, Bo Zhao, Juhyok Kim, Guangchao Sui, Jinming Shi
Summary: Receptors play key roles in oncogenic processes and are correlated with cancer patients' prognoses. Targeting receptors using natural compounds has gained interest for cancer therapy. This review summarizes the classification of receptors, natural compounds targeting these receptors, and the mechanisms of their anti-cancer activities. The aim is to provide primary knowledge for mechanistic regulation and clinical applications of cancer therapies through targeting deregulated receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Wenmeng Wang, Shiyao Qiao, Guangyue Li, Jiahui Cheng, Cuicui Yang, Chen Zhong, Daniel B. Stovall, Jinming Shi, Chunbo Teng, Dangdang Li, Guangchao Sui
Summary: It has been discovered that YY1 can activate target gene expression by forming liquid-liquid phase separation condensates to assemble enhancers and super-enhancers. The histidine cluster in YY1's transactivation domain plays a crucial role in this process.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Umar Farooq, Hao Wang, Jingru Hu, Guangyue Li, Shah Jehan, Jinming Shi, Dangdang Li, Guangchao Sui
Summary: Polydatin (PD) is a natural compound that has anticancer activities, but its mechanisms of action are not well understood. PD selectively inhibited the proliferation of hepatocellular carcinoma (HCC) cells while showing low toxicity in normal cells. Additionally, PD enhanced the inhibitory effects of doxorubicin (DOX) and cisplatin (DDP) in HCC cells and reduced their toxicity in normal liver cells. RNA-seq studies revealed multiple altered signaling pathways in PD-treated HCC cells, and the expression of PD-repressed genes correlated with poor clinical outcomes in HCC patients. These findings improve our understanding of PD's anticancer mechanisms and suggest potential clinical approaches for HCC therapies.
Article
Biology
Guangyue Li, Gongbo Su, Yunxuan Wang, Wenmeng Wang, Jinming Shi, Dangdang Li, Guangchao Sui
Summary: G-quadruplexes in promoters play a regulatory role in DNA replication and gene transcription. They exhibit selective constraint and contribute to active gene expression by enriching gene activation histone marks, chromatin remodeler and transcription factor binding sites.
COMMUNICATIONS BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jiahui Cheng, Guangyue Li, Wenmeng Wang, Daniel B. Stovall, Guangchao Sui, Dangdang Li
Summary: As ubiquitous transcripts in eukaryotes, circRNAs are closed loop RNA molecules without a cap and poly(A) tail. Initially considered non-coding RNA, circRNAs have been found to encode functional proteins. This review discusses their biogenesis, mRNA products, regulatory mechanisms, aberrant expression, and biological and clinical relevance in cancer.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2023)
Article
Oncology
Shiyao Qiao, Wenmeng Wang, Cheng Yi, Qingqing Xu, Wenfei Wang, Jinming Shi, Daniel B. Stovall, Dangdang Li, Guangchao Sui
Summary: YY1 regulates various cancer-related genes and activates key oncoproteins. The oncoprotein binding (OPB) domain of YY1 is necessary and stimulatory to its oligomerization. Hydrophobic residues, especially F219, in the OPB are essential to YY1 intermolecular interaction. Mutations of the hydrophobic residues showed better ability than wild-type YY1 in promoting breast cancer cell proliferation and migration. Further study revealed improved binding affinity of YY1 proteins with mutated hydrophobic residues to EZH2. Our data support the model of a mutually exclusive process between YY1 oligomerization and its regulation of oncoproteins.
Article
Oncology
Jialiang Li, Ke Shi, Tianqi Xu, Jingru Hu, Tianxin Li, Guangyue Li, Kuida Chen, Dangdang Li, Kazushi Inoue, Guangchao Sui
Summary: The tumor suppressor gene DMTF1 has three alternatively spliced mRNA isoforms encoding different protein variants, with beta and gamma isoforms exhibiting impaired mRNA stability and reduced protein expression. Both beta and gamma isoforms can associate with DMTF1 alpha and counteract its transactivation of the ARF promoter, suggesting oncogenic roles in breast cancer development.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2021)
