Article
Biochemistry & Molecular Biology
Elena R. Kingston, Lianne W. Blodgett, David P. Bartel
Summary: In this study, six sites that can direct miRNA degradation were identified in Drosophila cells or embryos, with five sites located in mRNAs and one site located in a noncoding RNA named Marge. These sites are able to degrade miRNAs without causing collateral target degradation, which helps explain the effectiveness of this miRNA-degradation pathway. Disruption of this pathway results in lethality, with many flies dying as embryos. The concomitant derepression of miR-3 and its paralog miR-309, as well as the loss of Marge-directed degradation of miR-310 miRNAs, are responsible for some of the lethal effects and defects in embryonic cuticle development. Therefore, TDMD is implicated in the viability and proper development of animals.
Article
Genetics & Heredity
Daiki Shikata, Shogo Matoba, Masashi Hada, Akihiko Sakashita, Kimiko Inoue, Atsuo Ogura
Summary: In this study, researchers identified RLTR45-int subfamily of ERVK as a strong reprogramming barrier for SCNT. This reprogramming barrier not only suppressed the expression of RLTR45-int elements, but also inhibited the expression of nearby genes, further affecting the development and survival of embryos.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Yanhua Song, Gaopeng Hou, Jonathan Diep, Yaw Shin Ooi, Natalia S. Akopyants, Stephen M. Beverley, Jan E. Carette, Harry B. Greenberg, Siyuan Ding
Summary: ING3 plays a crucial role in silencing ERVs by establishing EZH2-mediated H3K27 trimethylation modification, leading to the induction of MDA5-MAVS-interferon signaling and functional inhibition of several virus infections. Loss of ING3 results in decreased H3K27 trimethylation enrichment at ERVs, demonstrating an important new function of ING3 in ERV silencing and contributing to innate immune regulation in somatic cells.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Isabel Duarte, Gil Carraco, Nayara T. D. de Azevedo, Vladimir Benes, Raquel P. Andrade
Summary: MicroRNAs in chick embryos have been identified and shown to potentially play important roles in regulating early vertebrate embryo development. This discovery is significant for the avian research community and can lead to further experimental testing of new miRNAs.
Article
Genetics & Heredity
Christopher Wong, Pratik S. Kadekar, Elena S. Jurczak, Richard S. Roy
Summary: During environmental challenges, organisms can temporarily halt reproduction and enter a diapause stage. In C. elegans, the activation of AMPK in neurons plays a critical role in controlling germ cell development quiescence during the dauer stage. Genetic analysis revealed that AMPK activation in neurons leads to changes in RAB-7 trafficking through the regulation of TBC-7, a predicted RabGAP protein. This cellular trafficking pathway, regulated by AMPK and microRNAs, controls germline gene expression cell non-autonomously in response to adverse environmental conditions.
Article
Multidisciplinary Sciences
Odei Barrenada, Eduardo Larriba, Daniel Fernandez-Perez, Miguel angel Brieno-Enriquez, Jesus del Mazo Martinez
Summary: Recent studies have shown that mitochondrial small noncoding RNAs (sncRNAs) play important roles in germ cell development and differentiation. By analyzing sequencing data, we identified the origins, expression levels, and potential functions of piRNAs, as well as their association with other sncRNAs and the mitochondrial regulatory region. Our findings suggest that mitochondria-associated piRNAs may mediate nucleo-mitochondrial communication.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Omid Karami, Cheryl Philipsen, Arezoo Rahimi, Annisa Ratna Nurillah, Kim Boutilier, Remko Offringa
Summary: The study reveals that endogenous auxin is crucial for maintaining embryonic cell identity and the formation of multicellular proembryos during somatic embryogenesis induced by 2,4-D and AHL15 in Arabidopsis.
Article
Multidisciplinary Sciences
Jian Huang, Lei Zhao, Shikha Malik, Benjamin R. Gentile, Va Xiong, Tzahi Arazi, Heather A. Owen, Jiri Friml, Dazhong Zhao
Summary: This study demonstrates that spatially restricted auxin signaling is necessary for promoting megaspore mother cell (MMC) specification in plants, and miR160 and ARF17 play key roles in this process. These findings are important for our understanding of the fate determination of reproductive cells in plants.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Tingting Li, Weigao Yuan, Shuai Qiu, Jisen Shi
Summary: This study examined 10 reference genes in synchronized somatic embryogenic and subsequent germinative cultures of Liriodendron hybrids using quantitative real-time reverse transcription PCR. Different suitable reference genes were identified for SE tissues and germinative organ tissues, providing guidance for future gene expression studies and functional analyses of SE in Liriodendron hybrids as well as early embryonic gene expression analyses in other woody plant species.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Xiaonan Xu, Kaizhen Wang, Olga Vera, Akanksha Verma, Neel Jasani, Ilah Bok, Olivier Elemento, Dongliang Du, Xiaoqing Yu, Florian A. Karreth
Summary: The study reveals that ceRNAs play a crucial role in the oncogenic effects of somatic copy-number alterations (CNA), sequestering tumor suppressor microRNAs and promoting cancer progression and metastasis.
Article
Biochemistry & Molecular Biology
Xin Zhao, Xiaomin Li, Haiyang Sun, Xuan Zhao, Tingting Gao, Panpan Shi, Fuquan Chen, Lin Liu, Xinyi Lu
Summary: Dot1l is a histone methyltransferase responsible for H3K79 methylation, but it also participates in gene expression silencing. In this study, we found that Dot1l represses endogenous retroviruses and activates the 2-cell-like state in embryonic stem cells (ESCs). Mechanistically, Dot1l interacts with Npm1 to suppress MERVL expression by regulating protein level and deposition of histone H1. Our findings reveal a non-canonical transcriptional repressive role of Dot1l in ESCs.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Cell Biology
Jenna K. Schmidt, Kathryn M. Jones, Trevor Van Vleck, Marina E. Emborg
Summary: This article discusses the current methods and limitations for producing nonhuman primate genetic models that accurately replicate human disease, and reflects on how to ethically maximize the translational relevance of these models in the search for new therapeutic strategies to treat human disease.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Zi Liang, Liyan Liu, Xinyi Guo, Xia Wu, Yun-Li Yu, Ziyang Yu, Xiaolong Hu, Xing Zhang, Ji Wang
Summary: This study revealed differentially expressed circRNAs in iCCA tissues, suggesting that circRNAs may play crucial roles in iCCA pathogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Xiaolu Wei, Danna G. Eickbush, Iain Speece, Amanda M. Larracuente
Summary: The text discusses the important roles of satDNAs in heterochromatin formation and chromosome segregation, and how they are regulated by piRNAs.
Article
Cell Biology
Qin Wang, Jiena Liu, Zilong You, Yanling Yin, Lei Liu, Yujuan Kang, Siwei Li, Shipeng Ning, Hui Li, Yajie Gong, Shouping Xu, Da Pang
Summary: The study revealed that TINCR is upregulated in human breast cancer tissues and its knockdown suppresses tumorigenesis in vitro and in vivo. Mechanistically, TINCR recruits DNMT1 to the miR-503-5p locus promoter, increasing methylation and suppressing transcriptional expression of miR-503-5p. Additionally, TINCR functions as a competing endogenous RNA to upregulate EGFR expression by sponging miR-503-5p.
CELL DEATH & DISEASE
(2021)
Article
Plant Sciences
Emilija Hristova, Kateryna Fal, Laurin Klemme, David Windels, Etienne Bucher
Article
Plant Sciences
Gregory S. Jensen, Kateryna Fal, Olivier Hamant, Elizabeth S. Haswell
JOURNAL OF EXPERIMENTAL BOTANY
(2017)
Review
Virology
Mireia Ferrer, Simon Henriet, Celia Chamontin, Sebastien Laine, Marylene Mougel
Article
Biochemistry & Molecular Biology
Wei Deng, Simon Henriet, Daniel Chourrout
Article
Biochemistry & Molecular Biology
Magali Naville, Simon Henriet, Ian Warren, Sara Sumic, Magnus Reeve, Jean-Nicolas Volff, Daniel Chourrout
Article
Developmental Biology
Kateryna Fal, Matthieu Cortes, Mengying Liu, Sam Collaudin, Pradeep Das, Olivier Hamant, Christophe Trehin
Article
Biochemistry & Molecular Biology
Simon Henriet, Berta Colom Sanmarti, Sara Sumic, Daniel Chourrout
Review
Biochemistry & Molecular Biology
Kateryna Fal, Denisa Tomkova, Gilles Vachon, Marie-Edith Chaboute, Alexandre Berr, Cristel C. Carles
Summary: This review discusses the advantages and limitations of tools and technologies developed for impacting epigenetic marks in functional epigenomics. The methods range from drug inhibitors and nanobodies to engineered proteins targeting specific chromatin regions, with recent developments focusing on more precise and flexible tool designs. Proof-of-concept studies also indicate future potential for even finer, inducible/switchable systems for temporal analyses of molecular events following changes in chromatin marks.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Kateryna Fal, Niklas Korsbo, Juan Alonso-Serra, Jose Teles, Mengying Liu, Yassin Refahi, Marie-Edith Chaboute, Henrik Jonsson, Olivier Hamant
Summary: The study reveals that organogenesis in plants generates compression that has global effects on chromatin in individual cells, providing further evidence of the role of forces on chromatin changes. The research also shows that the presence of active nuclear compression in the domain where organ outgrowth occurs, suggesting a link between mechanical stress and chromatin alterations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Zoology
Simon Henriet, Anne Aasjord, Daniel Chourrout
Summary: Fritillaria and Oikopleura, closely related genera, exhibit major morphological differences in their adult forms that originate from a key developmental stage during larval organogenesis. In this stage, the epidermis of Fritillaria larvae retracts from the posterior digestive organs and establishes territories for house production.
FRONTIERS IN ZOOLOGY
(2022)
Article
Plant Sciences
Kateryna Fal, Alexandre Berr, Marie Le Masson, Adi Faigenboim, Emeline Pano, Nickolay Ishkhneli, Netta-Lee Moyal, Claire Villette, Denisa Tomkova, Marie-Edith Chaboute, Leor Eshed Williams, Cristel C. Carles
Summary: Chromatin is a dynamic platform that regulates gene expression through epigenetic modifications, particularly targeting histone H3 amino acid residues. This study focuses on the function of lysine 27 of H3 (H3K27), specifically its trimethylation by the Polycomb Repressive Complex 2 (PRC2), in controlling developmental genes in plants. By expressing a non-modifiable variant of H3 at residue K27 (H3.3(K27A)) in Arabidopsis, the researchers discovered novel roles of H3K27 in plant cell fates, metabolic pathways, and the elongation and lignin composition of the stem.
Article
Developmental Biology
Kateryna Fal, Mengying Liu, Assem Duisembekova, Yassin Refahi, Elizabeth S. Haswell, Olivier Hamant
Article
Biochemistry & Molecular Biology
Kateryna Fal, Benoit Landrein, Olivier Hamant
PLANT SIGNALING & BEHAVIOR
(2016)