From Cells to Virus Particles: Quantitative Methods to Monitor RNA Packaging

Q&A: What are exosomes, exactly?

(2016) James R. Edgar   BMC BIOLOGY

Imaging HIV-1 RNA dimerization in cells by multicolor super-resolution and fluctuation microscopies

(2016) Mireia Ferrer et al.   NUCLEIC ACIDS RESEARCH

Requirements for nucleocapsid-mediated regulation of reverse transcription during the late steps of HIV-1 assembly

(2016) Pierre-Jean Racine et al.   Scientific Reports

A retrovirus packages nascent host noncoding RNAs from a novel surveillance pathway

(2015) Matthew J. Eckwahl et al.   GENES & DEVELOPMENT

HIV-1 RNA genome dimerizes on the plasma membrane in the presence of Gag protein

(2015) Jianbo Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus duringDe NovoPrimary Infection of Human B and Endothelial Cells

(2015) Pravinkumar Purushothaman et al.   JOURNAL OF VIROLOGY

HIV-1 nucleocapsid and ESCRT-component Tsg101 interplay prevents HIV from turning into a DNA-containing virus

(2014) Célia Chamontin et al.   NUCLEIC ACIDS RESEARCH

Structural basis of genomic RNA (gRNA) dimerization and packaging determinants of mouse mammary tumor virus (MMTV)

(2014) Suriya J Aktar et al.   Retrovirology

MLV requires Tap/NXF1-dependent pathway to export its unspliced RNA to the cytoplasm and to express both spliced and unspliced RNAs

(2014) Lucie Pessel-Vivares et al.   Retrovirology

Life of psi: How full-length HIV-1 RNAs become packaged genomes in the viral particles

(2014) Malika Kuzembayeva et al.   VIROLOGY

Combining Single RNA Sensitive Probes with Subdiffraction-Limited and Live-Cell Imaging Enables the Characterization of Virus Dynamics in Cells

(2013) Eric Alonas et al.   ACS Nano

TANGO: a generic tool for high-throughput 3D image analysis for studying nuclear organization

(2013) Jean Ollion et al.   BIOINFORMATICS

FISH-quant: automatic counting of transcripts in 3D FISH images

(2013) Florian Mueller et al.   NATURE METHODS

Dual Analysis of the Murine Cytomegalovirus and Host Cell Transcriptomes Reveal New Aspects of the Virus-Host Cell Interface

(2013) Vanda Juranic Lisnic et al.   PLoS Pathogens

A comprehensive evaluation of normalization methods for Illumina high-throughput RNA sequencing data analysis

(2012) M.-A. Dillies et al.   BRIEFINGS IN BIOINFORMATICS

Identification of a Minimal Region of the HIV-1 5′-Leader Required for RNA Dimerization, NC Binding, and Packaging

(2012) Xiao Heng et al.   JOURNAL OF MOLECULAR BIOLOGY

HIV-2 Genome Dimerization Is Required for the Correct Processing of Gag: a Second-Site Reversion in Matrix Can Restore Both Processes in Dimerization-Impaired Mutant Viruses

(2012) A. L'Hernault et al.   JOURNAL OF VIROLOGY

Immunolabeling artifacts and the need for live-cell imaging

(2012) Ulrike Schnell et al.   NATURE METHODS

Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

(2012) Sami Hocine et al.   NATURE METHODS

Sequences within Both the 5′ UTR and Gag Are Required for Optimal In Vivo Packaging and Propagation of Mouse Mammary Tumor Virus (MMTV) Genomic RNA

(2012) Farah Mustafa et al.   PLoS One

Application of Live-Cell RNA Imaging Techniques to the Study of Retroviral RNA Trafficking

(2012) Darrin V. Bann et al.   Viruses-Basel

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

(2012) Kishanda Vyboh et al.   Jove-Journal of Visualized Experiments

Small Nucleolar RNAs U32a, U33, and U35a Are Critical Mediators of Metabolic Stress

(2011) Carlos I. Michel et al.   Cell Metabolism

Structural Determinants and Mechanism of HIV-1 Genome Packaging

(2011) Kun Lu et al.   JOURNAL OF MOLECULAR BIOLOGY

Role of HIV-1 RNA and protein determinants for the selective packaging of spliced and unspliced viral RNA and host U6 and 7SL RNA in virus particles

(2011) L. Didierlaurent et al.   NUCLEIC ACIDS RESEARCH

High-resolution human cytomegalovirus transcriptome

(2011) D. Gatherer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell biology of retroviral RNA packaging

(2011) Nolwenn Jouvenet et al.   RNA Biology

Mutations in matrix and SP1 repair the packaging specificity of a Human Immunodeficiency Virus Type 1 mutant by reducing the association of Gag with spliced viral RNA

(2010) Natalia Ristic et al.   Retrovirology

Murine leukemia virus RNA dimerization is coupled to transcription and splicing processes

(2010) Stéphan Maurel et al.   Retrovirology

Imaging the interaction of HIV-1 genomes and Gag during assembly of individual viral particles

(2009) Nolwenn Jouvenet et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High efficiency of HIV-1 genomic RNA packaging and heterozygote formation revealed by single virion analysis

(2009) J. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamics of HIV-1 Assembly and Release

(2009) Sergey Ivanchenko et al.   PLoS Pathogens

Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline

(2008) Partha P. Das et al.   MOLECULAR CELL

Imaging the biogenesis of individual HIV-1 virions in live cells

(2008) Nolwenn Jouvenet et al.   NATURE