Article
Biochemical Research Methods
Seyyed Mehdy Elahi, Nazila Nazemi-Moghaddam, Christine Gadoury, Julie Lippens, Stevo Radinovic, Marie-Helene Venne, Anne Marcil, Renald Gilbert
Summary: The FFA method for titrating AdV using new monoclonal antibodies and automatic counting of positive cells is more precise and reliable compared to traditional methods.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Nasir Uddin Ahmed, Abul Khair, Jayedul Hassan, Md Abu Hadi Noor Ali Khan, A. K. M. Anisur Rahman, Warda Hoque, Mustafizur Rahman, Nobumichi Kobayashi, Michael P. Ward, Md Mahbub Alam
Summary: This study aimed to identify risk factors for BRV infection and determine the G and P genotypes of BRV strains in diarrheic calves in Bangladesh. Through the analysis of 200 fecal samples from diarrheic calves, risk factors for BRV infection were found to include district, age, and breed of calves. The predominant genotype of BRV was identified as G6P[11].
Article
Biotechnology & Applied Microbiology
Mingyue Cao, Lijun Shi, Peng Peng, Bo Han, Lin Liu, Xiaoqing Lv, Zhu Ma, Shengli Zhang, Dongxiao Sun
Summary: This study investigated the genetic effects of the HTR1B gene on milk fatty acid traits in dairy cattle. Multiple SNPs were found to be significantly associated with medium-chain saturated fatty acids, and haplotype analysis revealed strong associations with specific fatty acids. A missense mutation in exon 1 and SNPs in the promoter region were predicted to alter protein structure and transcription factor binding sites, suggesting potential mechanisms for the genetic effects observed.
Article
Microbiology
Xiaolei Ren, Waqar Saleem, Robin Haes, Jiexiong Xie, Sebastiaan Theuns, Hans J. Nauwynck
Summary: This study found that VP8* protein of porcine rotavirus specifically binds to glycans with a β-lactose structure, and lactose can significantly reduce the infectivity of specific viral strains, thus playing a protective role in preventing infection in piglets.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Antonio Arenas, Carmen Borge, Alfonso Carbonero, Ignacio Garcia-Bocanegra, David Cano-Terriza, Javier Caballero, Antonio Arenas-Montes
Summary: After a year of the SARS-CoV-2 epidemic, there is still no specific effective treatment, so finding pharmacological and immunological mechanisms to control the infection is important. Using cow's milk immune to BCoV as a possible control measure against COVID-19, due to the close phylogenetic relationship between COVID-19 and BCoV viruses, can help with specific immune response.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Veterinary Sciences
Junzhen Chen, Dan Li, Yafang Xu, Zeyu Li, Siqi Ma, Xinyi Liu, Yuanyuan Yuan, Chengyuan Zhang, Qiang Fu, Huijun Shi
Summary: This study optimized dual and multiplex ddPCR methods for the detection of bovine enterovirus, bovine coronavirus, and bovine rotavirus infections. The results showed that these methods were more effective than conventional qPCR methods.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Food Science & Technology
Mutamed Ayyash, Abdelmoneim Abdalla, Basim Abu-Jdayil, Thom Huppertz, Raman Bhaskaracharya, Saleha Al-Mardeai, Anusha Mairpady, Arachchige Ranasinghe, Anas Al-Nabulsi
Summary: The study found that high-pressure processing and ultra-high temperature treatment can enhance the rheological properties of fermented camel milk, with the lowest viscosity observed in fermented milk treated with low-temperature long-time and high-temperature short-time methods.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ralf Weiskirchen, Sarah K. Schroeder, Sabine Weiskirchen, Eva Miriam Buhl, Bodo Melnik
Summary: Extracellular vesicles, such as exosomes, are small bilayered biovesicles released by cells, enriched with biologically active components. Milk exosomes play a key role in infant growth and development, while exosomes derived from different cell types have regenerative and immunomodulatory properties. Isolation protocols for milk exosomes allow the purification of highly enriched fractions, expressing typical exosomal protein markers.
Article
Public, Environmental & Occupational Health
Ye Wang, Yuling Zheng, Yan Li, Shengwei Zhang, Xin Wang, Huijun Zong, Wenhua Huang, Decong Kong, Yongqiang Jiang, Peng Liu, Qingyu Lv, Hua Jiang
Summary: In this study, a rapid, accurate, and sensitive AlphaLISA method was developed and evaluated for the detection of rotavirus infection. The results showed good consistency with the gold standard RT-qPCR method. AlphaLISA can be used for the rapid detection of rotavirus infection in clinical practice.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Chemistry, Applied
Rongbo Fan, Shubin Xie, Shifeng Wang, Zhongna Yu, Xueheng Sun, Qijing Du, Yongxin Yang, Rongwei Han
Summary: This study identified markers for detecting adulteration in goat milk using proteomics and metabolomics methods. SCGB1D, uric acid, and N-formylkynurenine were found to be potential markers at different adulteration levels. These findings can aid in assessing the authenticity of goat milk.
Article
Food Science & Technology
Mengjie Zhang, Xiaomeng Sun, Jianjun Cheng, Mingruo Guo
Summary: This study analyzed and compared the differences in proteins, fatty acids, minerals, and basic chemical composition between canine, bovine, and caprine milk. Canine milk contains higher contents of protein, fat, and ash, and a lower content of lactose compared to bovine and caprine milk. The protein and fatty acid profiles of canine milk are different from those of bovine and caprine milk. Canine milk also has higher levels of Mg, K, Na, Fe, and Zn.
Review
Agriculture, Dairy & Animal Science
Ludmila Krizova, Veronika Krestakova, Katerina Dadakova, Tomas Kasparovsky
Summary: Milk and dairy products are important sources of isoflavones, including equol, which can be adjusted in content through feedstuffs and processing methods to provide a source of this metabolite for humans who cannot produce it themselves.
Article
Microbiology
Apostolos S. Angelidis, Afroditi S. Grammenou, Charalampos Kotzamanidis, Nektarios D. Giadinis, Antonios G. Zdragas, Daniel Sergelidis
Summary: This study aimed to estimate the prevalence of L. monocytogenes in bovine bulk-tank milk in Greece and characterize the isolates in terms of pathogenic determinants, biofilm-forming ability, and antimicrobial susceptibility. Out of 138 samples, five (3.6%) tested positive for L. monocytogenes, with low populations. Most isolates belonged to serogroups 1/2a, 3a and carried virulence genes, while biofilm-forming ability and antimicrobial resistance varied. The study highlights the importance of continued monitoring of this pathogen in farm animals due to its public health threat.
Article
Nanoscience & Nanotechnology
Hong En Fu, Thomas Myeongseok Koo, Myeong Soo Kim, Min Jun Ko, Bum Chul Park, Kyuha Oh, Younhyung Cho, Jae-Wan Jung, Sungil Kim, Woong Sik Jang, Chae Seung Lim, Young Keun Kim
Summary: This study aimed to establish a magnetic-fluorescent nanocluster for high-sensitivity lateral flow assay (LFA) to detect rotavirus antigen. The results showed that the developed LFA platform outperformed commercial test kits, with the potential to inhibit virus propagation.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Applied
Xin Wang, Aiqian Ye, Anant Dave, Harjinder Singh
Summary: Plant-based milk alternatives are gaining popularity due to sustainability and health benefits. In vitro digestion study showed that oat milk remained stable, while oat milk-bovine skim milk blend formed coagulated particles, impacting gastric emptying rate and nutrient release.
FOOD HYDROCOLLOIDS
(2022)
Article
Behavioral Sciences
Alexandra Lundquist, Brent A. McBride, Sharon M. Donovan, Maris Wszalek
Summary: This study aimed to understand and describe the perceptions of first-time mothers with prenatal intentions to breastfeed and utilize childcare, of paternal support for the breastfeeding experience. The findings suggest that the breastfeeding experience of first-time mothers is shaped by the actions, behaviors, and beliefs of their partners, and that there is a lack of knowledge on how to optimally involve fathers in breastfeeding.
Article
Nutrition & Dietetics
Victoria C. Daniels, Marcia H. Monaco, Mei Wang, Johanna Hirvonen, Henrik Max Jensen, Arthur C. Ouwehand, Ratna Mukherjea, Ryan N. Dilger, Sharon M. Donovan
Summary: This study investigated the effects of supplementing piglet formula with 2'-fucosyllactose (2'-FL) and Bifidobacterium longum subsp. infantis Bi-26 (Bi-26) on piglet growth and intestinal development. The results showed that the addition of 2'-FL and/or Bi-26 supported piglet growth without detrimental effects on body weight, organ weight, or intestinal structure and function.
Article
Cell Biology
No-Joon Song, Aejin Lee, Rumana Yasmeen, Qiwen Shen, Kefeng Yang, Shashi Bhushan Kumar, Danah Muhanna, Shanvanth Arnipalli, Sabrena F. Noria, Bradley J. Needleman, Jeffrey W. Hazey, Dean J. Mikami, Joana Ortega-Anaya, Rafael Jimenez-Flores, Jeremy Prokop, Ouliana Ziouzenkova
Summary: This study reveals that epiregulin (EREG) regulates glucose uptake by activating leptin receptor (LepR) under leptin-deficient conditions. EREG promotes glucose uptake through ERK activation and GLUT4 translocation to the cell surface. This mechanism is independent of obesity in mice.
Article
Pharmacology & Pharmacy
Aejin Lee, McKensie L. Mason, Tao Lin, Shashi Bhushan Kumar, Devan Kowdley, Jacob H. Leung, Danah Muhanna, Yuan Sun, Joana Ortega-Anaya, Lianbo Yu, Julie Fitzgerald, A. Courtney DeVries, Randy J. Nelson, Zachary M. Weil, Rafael Jimenez-Flores, Jon R. Parquette, Ouliana Ziouzenkova
Summary: This study compares the effects of free insulin to insulin bound to AACs nanofibers in type 1 diabetes models. The results show that the AAC2-hINS complex significantly improves glucose levels, insulin levels, and neurotransmitter expression, and enhances physical and cognitive performance.
Review
Agriculture, Dairy & Animal Science
Brianda D. Gonzalez-Orozco, Israel Garcia-Cano, Rafael Jimenez-Flores, Valente B. Alvarez
Summary: Kefir, a fermented dairy product with probiotic properties, has shown promising antimicrobial effects through its microbiota and associated compounds, which may act through various mechanisms. While research on kefir's health properties exists, more studies are needed to further understand the antimicrobial activity of its microbiota and compounds.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
G. Ortiz Gonzalez, R. Jimenez Flores, D. R. Bremmer, J. H. Clark, E. J. DePeters, S. J. Schmidt, J. K. Drackley
Summary: Modification of milk fat composition can alter manufacturing characteristics or produce dairy products low in saturated fat. This study evaluated the functional properties of cream obtained from milks with modified fat composition. Results showed that the optimal functionality of cream depends more on its content of palmitic acid than on unsaturated fatty acids.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Da Chen, Diana Rocha-Mendoza, Shengyue Shan, Zachary Smith, Israel Garcia-Cano, Julie Prost, Rafael Jimenez-Flores, Osvaldo Campanella
Summary: This study compared the physicochemical properties, in vitro digestion, and cellular uptake between meat analogues (MAs) and chicken breasts (CB). It was found that MAs had differences in hardness and texturization compared to CB, and the soluble peptides in MAs had higher molecular weight and hydrophobicity after digestion. Both MAs and CB showed no cytotoxicity or inflammatory response to Caco-2 cells, with MAs exhibiting lower peptide permeability across the cells. Liquid chromatography with tandem mass spectrometry revealed that the identified peptides in MAs and CB contained different numbers of amino acid residues, with MAs having fewer essential and non-essential amino acids in the permeate compared to CB.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Igor Splichal, Sharon M. Donovan, Zdislava Kindlova, Zbynek Stranak, Vera Neuzil Bunesova, Marek Sinkora, Katerina Polakova, Barbora Valaskova, Alla Splichalova
Summary: Gnotobiotic animals with defined microbiota were used to study host-microbiota and microbiota-microbiota interferences. Preterm germ-free piglets were mono-associated with probiotic Bifidobacterium animalis subsp. lactis BB-12 (BB12) to prevent the consequences of Salmonella Typhimurium infection. BB12 partially ameliorated the inflammatory response to LT2 infection by preserving goblet cell density in the colon and decreasing the expression of inflammatory markers in the jejunum, ileum, colon, and plasma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Behavioral Sciences
Tianying Cai, Carolyn Sutter, Sharon M. Donovan, Barbara H. Fiese
Summary: The study aimed to investigate the relationship between mother-infant sleep duration for the first 2 years of life, whether this relationship changes over time, and the factors influencing this relationship. The parents of 464 infants provided sleep duration and health information at 3, 12, 18, and 24 months postpartum. The findings showed two sleep profiles (low maternal sleep and average maternal sleep) and a transition from low to average sleep profile after 12 months. The study highlights the importance of establishing healthy sleep routines for mothers and infants in the first year to promote long-term sleep hygiene.
JOURNAL OF DEVELOPMENTAL AND BEHAVIORAL PEDIATRICS
(2023)
Article
Chemistry, Applied
Kaavya Rathnakumar, Joana Ortega-Anaya, Rafael Jimenez-Flores, Sergio I. Martinez-Monteagudo
Summary: This study extracted milk phospholipids (MPLs) from beta-serum using a tertiary amine (N-N dimethylcyclohexylamine, CyNMe2). The extraction conditions (time, temperature, and sample-to-solvent ratio) were evaluated, and the extracted lipids were analyzed and quantified. The highest extraction yields of MPLs were obtained at 60 degrees C extraction for 18 hours with a sample-to-solvent ratio of 18 (w/w). The recovered fraction consisted of phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, and sphingomyelin.
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2023)
Article
Food Science & Technology
Holly A. Huellemeier, Necla M. Eren, Taylor D. Payne, Zachary D. Schultz, Rafael Jimenez-Flores, Dennis R. Heldman
Summary: The formation of proteinaceous fouling on both submicron and pilot scales is observed, with a gradient in fouling magnitude at both scales as a function of local residence time and temperature. The study shows that there is a scale-up factor of approximately 70-120 times when converting between QCM-D and pilot-scale fouling thicknesses. The similarities in foulant properties and fouling rates support the use of QCM-D as a viable technology for scale-up studies of milk fouling.
ACS FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Kaavya Rathnakumar, Joana Ortega-Anaya, Rafael Jimenez-Flores, Sergio I. Martinez-Monteagudo
Summary: The distribution of phospholipids within ice cream manufacturing process was studied using partition coefficients and confocal microscopy. The results showed that phospholipids were mainly located in the fat phase, and the addition of phospholipids increased viscosity and yield stress of the ice cream, delaying the onset and slowing down the rate of meltdown.
JOURNAL OF DAIRY SCIENCE
(2023)
Review
Nutrition & Dietetics
Yuting Fan, Arden L. McMath, Sharon M. Donovan
Summary: This review examines the impact of milk oligosaccharides (MOS) on brain and neurocognitive development in early life, synthesizing current literature from preclinical models and human observational studies. The study found that fucosylated and sialylated milk oligosaccharides play crucial roles in learning, memory, executive functioning, and brain structural development. However, limitations were identified, such as overlooking the complexity of naturally occurring MOS compositions and challenges in quantifying MOS intake in human studies.
Article
Microbiology
Brianda D. Gonzalez-Orozco, Tasha M. Santiago-Rodriguez, Israel Garcia-Cano, Rafael Jimenez-Flores, Valente B. Alvarez
Summary: This study presents the draft genome sequence and assembly of Lactobacillus helveticus OSU-BDGOAK2 and Lactobacillus kefiranofaciens OSU-BDGOA1 isolated from kefir grains, which exhibited in vitro antibacterial activity. Genome analysis revealed the presence of gene clusters encoding bacteriocins in both strains.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Food Science & Technology
Alba C. Mayta-Apaza, Diana Rocha-Mendoza, Israel Garcia-Cano, Rafael Jimenez-Flores
Summary: This study focuses on valorizing waste streams from the dairy and fisheries industries using fermentation. Previous results showed advantages of rapid acidification and high microbial activity in this fermentation. The study characterizes the peptides produced from this fermentation and provides valuable information for potential applications in industrial fermentation and efficient waste stream utilization.
ACS FOOD SCIENCE & TECHNOLOGY
(2022)
