Article
Immunology
Bahareh Vakili, Mahboubeh Karami-Darehnaranji, Esmaeil Mirzaei, Farnaz Hosseini, Navid Nezafat
Summary: Graphene, as a new type of vaccine adjuvant, has been found to enhance antigen immunogenicity and stimulate immune responses. However, its injection may lead to oxidative stress and inflammation, thereby requiring modifications to reduce adverse effects.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Hyunjung Kim, Jigui Yu, Di Bai, Moon H. H. Nahm, Pengfei Wang
Summary: Both VSA-1 and QS-21 can enhance immune responses, particularly beneficial for breakthroughs related to PCV13. Due to its accessibility and lower toxicity, VSA-1 can be included in a new vaccine formulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Neftali Ortega Alarcon, Maddy Jaramillo, Heidi M. Mansour, Bo Sun
Summary: This review summarizes the current progress of therapeutic subunit vaccines for cancer immunotherapy, including the classification of tumor antigens and the bioinformatic approaches for identifying tumor neoantigens. The targeting adjuvants based on pattern recognition receptors (PRRs) and their delivery platforms are extensively discussed, emphasizing the importance of the interplay between multiple adjuvants and their combined delivery in cancer immunotherapy.
Article
Pharmacology & Pharmacy
Myriam Lamrayah, Capucine Phelip, Celine Coiffier, Celine Lacroix, Thibaut Willemin, Thomas Trimaille, Bernard Verrier
Summary: This study investigates the potential of micelles composed of polylactide-block-poly(N-acryloxysuccinimide-co-N-vinylpyrrolidone) as a delivery system for vaccines. The micelles were non-toxic and efficiently taken up by immune cells. Coupling of antigenic protein p24 on the micelles improved its antigenicity. In mouse immunization, the micelles showed comparable immune response intensity to gold standards and higher avidity index over time.
Article
Oncology
Sapna P. Patel, Gina R. Petroni, Jason Roszik, Walter C. Olson, Nolan A. Wages, Kimberly A. Chianese-Bullock, Mark Smolkin, Nikole Varhegyi, Elizabeth Gaughan, Kelly T. Smith, Kathleen Haden, Emily H. Hall, Sacha Gnjatic, Patrick Hwu, Craig L. Slingluff
Summary: This study evaluated the safety and immunogenicity of a novel long peptide vaccine with different adjuvants, showing that the LPV7 vaccine induced certain immune responses under various adjuvant strategies, but did not significantly enhance T cell responses compared to vaccines using short peptides.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Immunology
Ana Carolina dos Reis Albuquerque Cajaraville, Mariana Pierre de Barros Gomes, Tamiris Azamor, Renata Carvalho Pereira, Patricia Cristina da Costa Neves, Paula Mello De Luca, Sheila Maria Barbosa de Lima, Luciane Pinto Gaspar, Elena Caride, Marcos da Silva Freire, Marco Alberto Medeiros
Summary: The attenuated yellow fever (YF) vaccine is highly effective but can cause rare fatal adverse events and has contraindications. Therefore, developing a non-live vaccine is important.
Article
Virology
Siva K. Gandhapudi, Hua Shi, Martin R. Ward, John Peyton Bush, Margarita Avdiushko, Karuna Sundarapandiyan, Lauren V. Wood, Mania Dorrani, Afsheen Fatima, Joe Dervan, Frank Bedu-Addo, Greg Conn, Ted M. Ross, Jerold G. Woodward
Summary: In this study, a novel pure enantio-specific cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (R-DOTAP) was evaluated as an immunomodulator for subunit vaccines capable of inducing both humoral and cellular-mediated immunity. R-DOTAP nanoparticles promoted strong cellular and antibody-mediated immune responses and protected mice from challenge with live SARS-CoV2 or influenza viruses.
Article
Immunology
Anna Morena D'Alise, Linda Nocchi, Irene Garzia, Laura Secli, Luigia Infante, Fulvia Troise, Gabriella Cotugno, Simona Allocca, Giuseppina Romano, Armin Lahm, Guido Leoni, Emanuele Sasso, Elisa Scarselli, Alfredo Nicosia
Summary: Virus vectored genetic vaccines (Vvgv) are a promising approach for immune protection against infectious diseases and cancer. However, combining adjuvants with genetic vaccines has been challenging. In this study, the researchers developed a novel approach using an adenovirus vector to synchronize the activity of the genetic adjuvant with the vaccine. The results showed that this approach enhanced immune responses to viral and tumor antigens, suggesting its potential for more effective genetic vaccines.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Lingxiao Zhang, Jing Zhao, Xi Hu, Chenhan Wang, Yingbo Jia, Chaojie Zhu, Shangzhi Xie, Jiyoung Lee, Fangyuan Li, Daishun Ling
Summary: This study reports a Zn2+-doped layered double hydroxide (Zn-LDH) immunomodulating adjuvant, which can neutralize the acidic tumor microenvironment and promote an immune response. The Zn-LDH induces immunogenic cell death and enhances the release of antigens to stimulate cytotoxic T lymphocytes. The experiments demonstrate that the Zn-LDH adjuvant alone can significantly inhibit the growth, recurrence, and metastasis of solid tumors.
ADVANCED MATERIALS
(2022)
Review
Oncology
Marit M. Melssen, Caroline T. Fisher, Craig L. Slingluff, Cornelis J. M. Melief
Summary: Water-in-oil emulsion incomplete Freund's adjuvant (IFA) has been widely used in vaccines, and the new generation Montanide adjuvants have been developed to reduce toxicity. Peptide vaccines in Montanide ISA-51 or ISA-720 can induce high antibody titers and durable effector T cell responses. CD4(+) T cell help and peptide affinity are important for an efficient T cell response. Additional inclusion of Toll-like receptor ligand can enhance the immune response.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Multidisciplinary Sciences
Rong Yang, Shanshan Zhou, Qibing Zhou
Summary: In vitro cancer cells treated with a new potent ICD inducer, NAP, followed by UVA irradiation, were effective tumor immunotherapy when combined with CpG adjuvant, leading to enhanced survival and activation of T cells in tumors.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Multidisciplinary Sciences
Laura A. J. Feather, Vinod Nadella, Elisabeth Kastner, Yvonne Perrie, Anthony C. Hilton, Andrew Devitt
Summary: Liposomes are versatile systems in vaccine technology, acting as antigen delivery systems and adjuvants. This study develops an in vitro screening tool to predict the in vivo efficacy of novel liposome formulations.
SCIENTIFIC REPORTS
(2022)
Article
Polymer Science
Adam M. Weiss, Marcos A. Lopez, Benjamin W. Rawe, Saikat Manna, Qing Chen, Elizabeth J. Mulder, Stuart J. Rowan, Aaron P. Esser-Kahn
Summary: Cationic polymers are widely used in various biotechnologies, but their properties can lead to toxic or immunogenic effects. In this study, we synthesized a library of 107 polymers with varying charge, hydrophobicity, and molecular weight. By screening for cytotoxicity and immunogenicity, we identified three compositional regions of interest and investigated the underlying mechanisms using confocal microscopy. The results showed that highly cationic polymers induced toxicity by disrupting the cellular plasma membrane, while high molecular weight, hydrophobic polymers activated the NLRP3 inflammasome, leading to an immunogenic response. The framework described in this study allows for the systematic characterization of cationic materials for a range of applications.
Review
Chemistry, Physical
Adelina-Gabriela Niculescu, Alexandru Mihai Grumezescu
Summary: Polymer-based nanoparticles customized for size, morphology, and surface properties are gaining attention for drug, biomolecule, and gene delivery. They protect payloads from degradation and sustain controlled drug release, reducing clearance and increasing stability, solubility, and optimal concentration at target sites, especially in vaccine development.
Review
Pharmacology & Pharmacy
William R. Lykins, Christopher B. Fox
Summary: In recent years, there has been increased interest in vaccine adjuvant development for new and emerging vaccine modalities. However, the development of vaccine adjuvants still faces challenges, including translation complexities, safety concerns, and sourcing raw materials at scale. This review explores various adjuvant technologies and provides recommendations for improving clinical success through early consideration of factors and application of tools in the vaccine development pipeline.
Article
Multidisciplinary Sciences
Badaut Cyril, Pimnitah Visitdesotrakul, Aurelie Chabry, Pascal Bigey, Bernard Tornyigah, Jocelyne Roman, Jules Alao Maroufou, Annick Amoussou, Blaise Serge Ayivi, Gratien Sagbo, Nicaise Tuikue Ndam, Andrew V. Oleinikov, Rachida Tahar
Summary: The study found significant IgG responses against the entire DC13, PF11_0521_ DBL beta 3_D4 domain, and peptides. The responses varied against different peptides and depended on the clinical status of children. The response was stronger at day 30, and mostly did not differ between CM group and UM group.
SCIENTIFIC REPORTS
(2021)
Correction
Multidisciplinary Sciences
Cyril Badaut, Pimnitah Visitdesotrakul, Aurelie Chabry, Pascal Bigey, Bernard Tornyigah, Jocelyne Roman, Jules Alao Maroufou, Annick Amoussou, Blaise Serge Ayivi, Gratien Sagbo, Nicaise Tuikue Ndam, Andrew V. Oleinikov, Rachida Tahar
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Marcela Coelho Silva Ribeiro, Marcelo Coutinho de Miranda, Pricila da Silva Cunha, Gracielle Ferreira Andrade, Gustavo de Oliveira Fulgencio, Dawidson Assis Gomes, Silvia Ligorio Fialho, Frederico Pittella, Christine Charrueau, Virginie Escriou, Armando Silva-Cunha
Summary: The study developed lipoplexes coated with hyaluronic acid for potential neuroprotective effect in a retinal light damage model. These systems successfully delivered siRNA molecules targeting caspase-3 and showed significant inhibition of caspase-3 expression without any retinal toxicity.
Article
Chemistry, Multidisciplinary
Cristina Ana Mocanu, Elena Valeria Fuior, Geanina Voicu, Daniela Rebleanu, Florentina Safciuc, Mariana Deleanu, Ioana Madalina Fenyo, Virginie Escriou, Ileana Manduteanu, Maya Simionescu, Manuela Calin
Summary: The newly developed Psel-lipo/shRAGE lipoplexes reduce the inflammatory processes associated with RAGE signaling and the progression of atherosclerosis in ApoE-deficient mice. Downregulation of RAGE employing these lipoplexes may represent a promising new targeted therapy to block atherosclerosis progression.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Pharmacology & Pharmacy
Asad Ur Rehman, Virginie Busignies, Marcela Coelho Silva Ribeiro, Nayara Almeida Lage, Pierre Tchoreloff, Virginie Escriou, Christine Charrueau
Summary: The incorporation of siRNA into nanocarriers is essential for intracellular delivery, but its fate in the gastrointestinal environment remains unclear. The study showed that freshly prepared siRNA lipoplexes maintained their efficacy in gastric fluid, while exposure to intestinal fluid led to decreased gene silencing. Tablet-formed lipoplexes protected siRNA in gastric fluid but released it in intestinal fluid, impacting gene silencing efficacy.
Review
Biochemistry & Molecular Biology
Malvina Pizzuto, Pablo Pelegrin, Jean-Marie Ruysschaert
Summary: The NLRP3 inflammasome is an important initiator of inflammation, and its activation is regulated by lipids. This review summarizes the mechanisms of NLRP3 inflammasome activation by different lipids and how lipids control the cellular localization of NLRP3 during activation.
PROGRESS IN LIPID RESEARCH
(2022)
Article
Food Science & Technology
Albert Ros-Lucas, Pascal Bigey, Jean-Philippe Chippaux, Joaquim Gascon, Julio Alonso-Padilla
Summary: Snakebite envenomation causes a significant number of deaths each year, and the production of antivenoms is often limited by their variable potency and accessibility. This study presents a method based on sequence conservation and protein structural features to prioritize therapeutic epitopes from snake venom. The selected epitopes are applicable to the most medically important snake species in West Sub-Saharan Africa and can be customized for venom sequence analysis in other regions.
Article
Chemistry, Multidisciplinary
Danielle Campiol Arruda, Anne-Marie Lachages, Helene Demory, Guillaume Escriou, Rene Lai-Kuen, Pierre-Yves Dugas, Celine Hoffmann, Stephanie Bessoles, Guillaume Sarrabayrouse, Angelo Malachias, Stephanie Finet, Pedro Lana Gastelois, Waldemar Augusto de Almeida Macedo, Armando da Silva Cunha, Pascal Bigey, Virginie Escriou
Summary: This study developed a novel lipid-polymer hybrid nanoparticle called Spheroplexes for efficient siRNA delivery. The nanoparticles consist of a hydrophobic PLGA matrix surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed. The Spheroplexes were shown to be stable and more efficient than siRNA lipoplexes for delivering siRNA to cultured cells.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Anesthesiology
Raquel Tonello, Wayne B. Anderson, Steve Davidson, Virginie Escriou, Lei Yang, Brian L. Schmidt, Wendy L. Imlach, Nigel W. Bunnett
Summary: This study suggests that endocytosis plays a key role in sensitization of nociceptors and synaptic transmission, and inhibiting endocytosis could be a potential therapeutic approach for pain treatment.
Article
Pharmacology & Pharmacy
Panpan Ma, Johanne Seguin, Nhu Ky Ly, Luis Castillo Henriquez, Eva Plansart, Karim Hammad, Rabah Gahoual, Helene Dhotel, Charlotte Izabelle, Bruno Saubamea, Cyrille Richard, Virginie Escriou, Nathalie Mignet, Yohann Corvis
Summary: This study reports the formulation, stability, and in vitro efficiency of Fisetin nanocrystals stabilized by poloxamer P407. The nanocrystals exhibit uniform size and high stability in various environments, and show improved cytotoxicity and anti-angiogenic effect compared to the free drug, suggesting promising clinical applications.
INTERNATIONAL JOURNAL OF PHARMACEUTICS-X
(2022)
Article
Biochemistry & Molecular Biology
Olivier Brison, Stefano Gnan, Dana Azar, Stephane Koundrioukoff, Rodrigo Melendez-Garcia, Su-Jung Kim, Melanie Schmidt, Sami El-Hilali, Yan Jaszczyszyn, Anne-Marie Lachages, Claude Thermes, Chun-Long Chen, Michelle Debatisse
Summary: The loose activation of the DNA-replication checkpoint causes instability in common fragile sites (CFSs), while tight checkpoint activation or CDK1 inhibition stabilizes them by advancing replication completion through extra replication-initiation events that depend on the availability of S-phase proteins involved in pre-RC building.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Review
Cell Biology
Malvina Pizzuto, Pablo Pelegrin
TRENDS IN CELL BIOLOGY
(2020)
Review
Chemistry, Multidisciplinary
Zhiyuan Shi, Yong Hu, Xin Li
Summary: Controlled drug delivery systems that can respond to mechanical force offer a unique solution for on-demand drug activation and release. Among various mechanical stimuli, ultrasound (US) has advantages in achieving spatiotemporally controlled drug release. Traditional US-triggered drug release relies on heat-induced phase transitions or chemical transformations, while the cutting-edge approach of Sonopharmacology leverages polymer mechanochemistry. The remaining challenges and potential future directions in this field are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Lijie Zheng, Yuanzheng Chen, Xun Gu, Yingying Li, Hanqing Zhao, Wenjun Shao, Tao Ma, Chuanbin Wu, Qingqing Wang
Summary: In this study, a novel dosage form consisting of dissolving microneedles and an adhesive transdermal patch was developed for the treatment of rheumatoid arthritis. In vitro and in vivo experiments demonstrated that the combination of drugs delivered by this dosage form effectively reduced joint inflammation and damage.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Kyungjik Yang, Hwa Seung Han, Seung Hwan An, Kyung Hoon Park, Keonwook Nam, Shinha Hwang, Yuyeon Lee, Sung Yeon Cho, Taehyung Kim, Deokyeong Choe, Sang Won Kim, Wonkyu Yu, Hyunah Lee, Jiyong Park, Sangguan You, Dong- Gyu Jo, Ki Young Choi, Young Hoon Roh, Jae Hyung Park
Summary: This study developed CP-loaded CS microcapsules to enhance the oral bioavailability of CP through controlled gastrointestinal delivery. The optimized microcapsules exhibited desirable physicochemical properties, showed anti-photoaging effects via antioxidant activity, and achieved controlled release in the gastrointestinal tract. This research provides a simple and economical approach for enhancing the oral bioavailability of CP for customized bioactive compound administration.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Huiyang Li, Shuo Liu, Wenjin Dai, Bingmei Yao, Yong Zhou, Sujia Si, Hairong Yu, Riguang Zhao, Fang Jin, Liqun Jiang
Summary: Changes in bodily fluid pressures are crucial in diseases like high-altitude pulmonary edema (HAPE). Researchers have developed hydrostatic pressure-sensitive multivesicular liposomes (PSMVLs) that can release drugs in response to pressure changes, with potential applications in HAPE treatment. Animal experiments showed that this system provides better protection for lung tissues and respiratory function, reducing the occurrence of pulmonary edema.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Qian Hu, Hongbing Lan, Yinmei Tian, Xiaonan Li, Mengmeng Wang, Jiao Zhang, Yulin Yu, Wei Chen, Li Kong, Yuanyuan Guo, Zhiping Zhang
Summary: Coacervate droplets formed through liquid-liquid phase separation have potential as delivery vesicles for therapeutics. However, their lack of physiological stability and membranes are challenges. In this study, polylysine-polynucleotide complex coacervate droplets with favorable stability were formulated to concentrate molecules and nanoparticles. Phospholipid membranes were further coated on the droplets to create coacervate-based artificial protocells (ArtPC) with membrane-like structures. These biofunctional ArtPC effectively reduced blood uric acid levels and prevented renal injuries.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Xiaowei Wang, Hongwei Lu, Fang Luo, Dan Wang, Apeng Wang, Xuelei Wang, Wenkai Feng, Xiaobo Wang, Jiayi Su, Mingliang Liu, Guimin Xia
Summary: Four novel lipid-like GEM diesters were synthesized and encapsulated into liposomes to improve the antitumor efficacy of Gemcitabine. The liposomes loaded with dimyristoyl GEM (LipodmGEM) showed enhanced cellular uptake, improved inhibition of cell migration, and a greatly extended half-life compared to free Gemcitabine. LipodmGEM successfully enriched the drug in the tumor and exhibited excellent anticancer efficacy in vivo with negligible systemic toxicity.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Yajing Sun, Ze Lu, John A. Taylor, Jessie L. S. Au
Summary: A recent development in cancer chemotherapy is the use of cytotoxics to induce tumor-specific immune response through immunogenic cell death (ICD). This study describes a method that utilizes immunostaining and machine-learning to identify cells with ecto-CRT in intact 3-dimensional tissues. The method was successfully applied to study drug-induced ICD in human bladder cancer.
JOURNAL OF CONTROLLED RELEASE
(2024)
Review
Chemistry, Multidisciplinary
Rafat Ali, Shantanu Sen, Rohil Hameed, Aamir Nazir, Sandeep Verma
Summary: This review provides a focused overview of emerging strategies for delivering gasotransmitters in a controlled and sustained manner to reestablish neurophysiological homeostasis.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Jing Chen, Xinyi Zhang, Jinshen Zhang, Zhaoxia Wang, Guilan Zhu, Ming Geng, Jinmiao Zhu, Yajun Chen, Wei Wang, Youcui Xu
Summary: In this study, a multifunctional responsive hydrogel system was developed for synergistic reoxygenation and chemo/photothermal therapy. The hydrogel system showed both therapeutic effects against metastatic breast cancer and wound infection, making it a promising strategy for treating and preventing tumor recurrence and associated wound infection.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Baoshan Huang, Na Zhang, Xinying Qiu, Rui Zeng, Shuimiao Wang, Mengxia Hua, Qing Li, Kaihui Nan, Sen Lin
Summary: This study revealed that robust ROS can oxidize mitochondrial DNA (ox-mtDNA) and cause its release into the cytosol, resulting in the activation of NLRP3 inflammasome. By using the mitochondria-targeted antioxidant SkQ1 and a novel mitochondria-targeted nanoparticle (SkQ1 NP), it was found that mitochondrial ROS scavenging could in situ inhibit DED-induced mtDNA oxidation and suppress NLRP3-mediated inflammation.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Wenqi Liu, Cheng Hu, Linyu Long, Shuyi He, Wen Zhang, Zhicun Wang, Li Yang, Yunbing Wang
Summary: Myocardial infarction is the leading cause of cardiovascular mortality, and current treatment methods have limitations. This study developed a smart carrier that can release different therapeutic substances for different pathological processes, effectively improving cardiac function, promoting cardiac repair, and preventing ventricular remodeling.
JOURNAL OF CONTROLLED RELEASE
(2024)
