Article
Mycology
Adans A. Colman, Harry C. Evans, Sara S. Salcedo-Sarmiento, Uwe Braun, Kifle Belachew-Bekele, Robert W. Barreto
Summary: The study described a new fungal species, Digitopodium hemileiae, which is a mycoparasite of coffee leaf rust. The research identified the potential applications of these fungi, including as biocontrol agents against coffee leaf rust, and reclassified species in different regions.
Article
Chemistry, Multidisciplinary
Fatemeh Farhangdoust, Feng Cheng, Wentao Liang, Yongmin Liu, Meni Wanunu
Summary: Compared to specific techniques, DNA sequencing does not require prior knowledge of the DNA sample. Current sequencing technologies require high inputs for library preparation, but electro-optical zero-mode waveguides (eZMWs) can efficiently load and rapidly identify DNA fragments, making them suitable for low-input DNA capture studies where native DNA is required.
ADVANCED MATERIALS
(2022)
Article
Plant Sciences
Qianwen Liu, Shuai Guo, Xiasheng Zheng, Xiaofeng Shen, Tianyi Zhang, Baosheng Liao, Wenrui He, Haoyu Hu, Ruiyang Cheng, Jiang Xu
Summary: A new method for determining the heterozygosity of licorice seed mixture was proposed, based on high-throughput full-length multiple DNA barcodes. The results showed that this method successfully identified the complete gradients of complex mixed samples. This method provides a brilliant and feasible way for evaluating the heterozygosity of licorice seeds.
Review
Plant Sciences
Shilpa, Rajni Kant Thakur, Pramod Prasad, S. C. Bhardwaj, O. P. Gangwar, Subodh Kumar
Summary: The outcome of host-pathogen interactions is influenced by both genetic and epigenetic systems.
Article
Chemistry, Multidisciplinary
Jinyue Zhang, Yu Wang, Yuqin Wang, Panke Zhang, Hong-Yuan Chen, Shuo Huang
Summary: DNA lesions induced by alkylating agents can be directly distinguished by observing the enzymatic stalling kinetics, which is not interfered with by the sequence context.
Article
Plant Sciences
Rimsha Ashraf, Eva Johansson, Pernilla Vallenback, Brian J. Steffenson, Prabin Bajgain, Mahbubjon Rahmatov
Summary: In this study, a wheat-rye multiple disomic substitution line, SLU126 4R (4D), 5R (5D), and 6R (7D), was used and crossed with Chinese Spring ph1b to induce homeologous recombination, resulting in introgressions with reduced rye chromosome segments that possessed resistance against wheat stripe rust. Through genotyping-by-sequencing (GBS) platforms and aligning putative SNPs to annotated rye NLR genes, the physical positions of NLR genes related to stripe rust resistance were identified. The study also confirmed the existence of a rye translocation containing the stripe resistance gene, which provides an additional asset for wheat improvement.
Article
Microbiology
Irene Gomez-de La Cruz, Karina Guillen-Navarro, Graciela Huerta-Palacios, Luz Veronica Garcia-Fajardo, Misael Martinez-Bolanos
Summary: This study evaluates the enzymatic activity of chitinase, glucanase, and protease in three native coffee leaf rust mycoparasite isolates from Chiapas, Mexico, and finds that hydrolytic enzymes play an important role in the interactions between these mycoparasites and CLR.
Article
Plant Sciences
Meng Li, Lu Hou, Chenxing Zhang, Weicong Yang, Xinru Liu, Hanqing Zhao, Xiaoming Pang, Yingyue Li
Summary: Autotetraploid sour jujube exhibits superior drought tolerance compared to diploid, possibly due to an increase in xylem vessels and parenchyma cells, as well as differential gene regulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Alejandro Scaffa, George A. Tollefson, Hongwei Yao, Salu Rizal, Joselynn Wallace, Nathalie Oulhen, Jennifer F. Carr, Katy Hegarty, Alper Uzun, Phyllis A. Dennery
Summary: This study reveals the potential DNA binding domains of HO-1 protein through experiments and structural modeling, and further demonstrates HO-1's ability to regulate the expression of target genes. This provides a foundation for developing specific drugs to modulate gene expression and cellular function.
Article
Food Science & Technology
Meng-Yue Zhou, Ran -Ran Xing, Ke-Han Liu, Yi-Qiang Ge, Ying Chen
Summary: This study aimed to develop a DNA barcoding method for the species identification of culinary spices. Five barcodes were evaluated to detect 19 culinary spices, but they could not definitively identify all the species. The combination of ITS2 and psbA-trnH barcodes improved the identification ability, successfully identifying all tested spices except for Cambodian cardamom. The method can be used to standardize labels on commercial culinary spices.
Article
Multidisciplinary Sciences
Guotai Yu, Oadi Matny, Nicolas Champouret, Burkhard Steuernagel, Matthew J. Moscou, Inmaculada Hernandez-Pinzon, Phon Green, Sadiye Hayta, Mark Smedley, Wendy Harwood, Ngonidzashe Kangara, Yajuan Yue, Catherine Gardener, Mark J. Banfield, Pablo D. Olivera, Cole Welchin, Jamie Simmons, Eitan Millet, Anna Minz-Dub, Moshe Ronen, Raz Avni, Amir Sharon, Mehran Patpour, Annemarie F. Justesen, Murukarthick Jayakodi, Axel Himmelbach, Nils Stein, Shuangye Wu, Jesse Poland, Jennifer Ens, Curtis Pozniak, Miroslava Karafiatova, Istvan Molnar, Jaroslav Dolezel, Eric R. Ward, T. Lynne Reuber, Jonathan D. G. Jones, Martin Mascher, Brian J. Steffenson, Brande B. H. Wulff
Summary: In this study, the authors assembled the genome of the wild diploid wheat relative Aegilops sharonensis and successfully cloned the stem rust resistance gene Sr62. The results show that Sr62 can be transferred to common wheat and provides high levels of resistance. This has significant implications for improving the disease resistance of wheat.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Andrea Spitaleri, Denis Garoli, Moritz Schuette, Hans Lehrach, Walter Rocchia, Francesco De Angelis
Summary: Single molecule protein sequencing has the potential to significantly impact proteomics and human biology, but faces challenges in its technological realization. Current protein sequencing techniques have limitations in sample amount and measurement range. Molecular dynamics simulations have shown that a re-engineered protein acting as a biological nanopore can accurately recognize different amino acids, paving the way for a new sequencing method.
Article
Plant Sciences
Jonathan Eagle, Yan Liu, Yukiko Naruoka, Weizhen Liu, Travis Ruff, Marcus Hooker, Sajal Sthapit, Elliott Marston, Karol Marlowe, Michael Pumphrey, Deven R. See
Summary: This study identified quantitative trait loci (QTL) for stripe rust resistance in wheat using a back-cross inbred-line population. A major QTL conferring all-stage resistance was identified on chromosome 1B, along with seven additional QTLs associated with adult plant resistance. This research provides valuable insights for improving resistance to stripe rust in wheat.
Article
Genetics & Heredity
Billy T. Lau, Alison Almeda, Marie Schauer, Madeline McNamara, Xiangqi Bai, Qingxi Meng, Mira Partha, Susan M. Grimes, HoJoon Lee, Gregory M. Heestand, Hanlee P. Ji
Summary: We developed a strategy using nanopore-based single-molecule sequencing to measure cfDNA methylomes, enabling longitudinal monitoring of cancer patients during treatment. This approach generated significantly more reads compared to existing methods and allowed us to determine the origin of individual reads. By leveraging the methylomes of matched tumors and immune cells, we characterized the cfDNA methylomes of cancer patients.
Article
Plant Sciences
Huan Guo, Hong Zhang, Guanghao Wang, Changyou Wang, Yajuan Wang, Xinlun Liu, Wanquan Ji
Summary: Wheat HSPs, encoded by conserved gene families, play important roles in plant development and stress responses. This study classified TaDnaJs and TaHsp70s into different groups and found diverse structural organization in TaDnaJ but a conserved J-domain. The expression patterns of HSP-encoding genes vary in response to different fungal stresses, indicating potential functional roles in wheat responses to fungal infections.