Article
Engineering, Biomedical
M. Bouyer, C. Garot, P. Machillot, J. Vollaire, V Fitzpatrick, S. Morand, J. Boutonnat, V Josserand, G. Bettega, C. Picart
Summary: The study suggests that using 3D-printed PLA scaffolds loaded with reduced doses of BMP-2 may be a safe and simple solution for large bone defects in the clinic. The dose of BMP-2 delivered from the scaffold significantly influences the amount of regenerated bone and the repair kinetics, showing good bone repair outcomes.
MATERIALS TODAY BIO
(2021)
Article
Materials Science, Multidisciplinary
Zhiming Zhang, Zijie Rong, Guofeng Wu, Yihan Wang, Zhiwen Tan, Juan Zheng, Yanglei Jin, Zhihao Liang, Chun Liu, Jiasong Guo, Lixin Zhu
Summary: The study introduced a controlled-oxygen-generating composite scaffold to enhance seed cell survival and improve bone defect reconstruction.
APPLIED MATERIALS TODAY
(2021)
Article
Biophysics
Jisun Park, Su Jeong Lee, Tae Gon Jung, Jun Hee Lee, Wan Doo Kim, Jae Young Lee, Su A. Park
Summary: Three-dimensional (3D) bioprinting allows for precise fabrication of scaffolds for tissue engineering, particularly those supporting cell attachment and osteogenic differentiation essential for bone tissue regeneration. Coating PCL scaffolds with PDA and depositing HA nanoparticles, followed by BMP-2 immobilization, promotes osteoblast proliferation and differentiation for potential bone tissue engineering applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Engineering, Biomedical
Xueqin Gao, Mintai P. Hwang, Nathaniel Wright, Aiping Lu, Joseph J. Ruzbarsky, Matthieu Huard, Haizi Cheng, Michael Mullen, Sudheer Ravuri, Bing Wang, Yadong Wang, Johnny Huard
Summary: This study compared the effectiveness of 5 bone morphogenetic proteins (BMPs) in promoting bone defect healing using a sustained-release platform. BMP2 and BMP7 were found to have the best bone regeneration capabilities.
Article
Materials Science, Multidisciplinary
Bo Chen, Zhengjie Lin, Qimanguli Saiding, Yongcan Huang, Yi Sun, Xinyun Zhai, Ziyu Ning, Hai Liang, Wei Qiao, Bingsheng Yu, W. K. Kelvin Yeung, Jie Shen
Summary: The use of three-dimensional (3D) printed biocomposite scaffolds, reinforced with magnesium oxide (MgO), has shown promising results in the treatment of critical-sized bone defects (CSD). The incorporation of magnesium ions (Mg2+) in the scaffold enhances mechanical performance and cytocompatibility, leading to improved bone regeneration and repair.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Multidisciplinary
Qian Liu, Xueliang Peng, Xiaojia Liu, Xiaohui Mou, Yayuan Guo, Lihua Yang, Yufei Chen, Yaying Zhou, Zhuoya Shi, Zhilu Yang, Zhuoyue Chen
Summary: BMPs, members of the TGF-8 superfamily, have been extensively studied for their osteoinductive ability and multiple functions. Current research focuses on BMP delivery systems to achieve local administration and delay degradation. Researchers have combined BMPs with other growth factors to enhance their effect during osteoinduction. This paper reviews the progress in BMP research, including structure and function, binding to various materials, cells, and growth factors. It also summarizes the applications of BMPs in bone defect repair and proposes suggestions for improving their clinical application.
COMPOSITES PART B-ENGINEERING
(2023)
Article
Materials Science, Textiles
Amir Nahanmoghadam, Maryam Asemani, Vahabodin Goodarzi, Somayeh Ebrahimi-Barough
Summary: A composite scaffold incorporating PHBV and PCL polymers with 5% HA nanoparticles was successfully fabricated for bone critical size defect regeneration. The scaffold showed enhanced bone regeneration and decreased fibrous connective tissue formation compared to other groups.
FIBERS AND POLYMERS
(2021)
Article
Multidisciplinary Sciences
Yue Wang, Xiaoyan Zhang, Shuang Mei, Yunlong Li, Anas Ameer Khan, Shuai Guan, Xiangjun Li
Summary: A rabbit model of mandibular box-shaped defects created through an intraoral approach was evaluated to determine the minimum size defect that would not spontaneously heal during the rabbit's natural life (critical-sized defect, CSD). The dimensions of the CSD were determined to be 8 mm x 3 mm x 2 mm. This model provides a clinically relevant base for future tissue engineering efforts in the mandible.
Article
Biophysics
Edoardo Borgiani, Georg N. Duda, Bettina M. Willie, Sara Checa
Summary: Critical-sized bone defects, when left untreated, can often lead to non-unions. Recombinant human BMP-2 treatment enhances bone tissue formation, but results in altered tissue patterning compared to regular bone healing. This study highlights how BMP-2 specific tissue patterning in critical-sized bone defects can be explained by mesenchymal stromal cell migration to regions with specific BMP-2 concentration.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ziyang Yang, Li Xie, Boqing Zhang, Gang Zhang, Fangjun Huo, Changchun Zhou, Xi Liang, Yujiang Fan, Weidong Tian, Yinghui Tan
Summary: This study demonstrates the potential of the BMP-2/PDA-BCP scaffold for the treatment of small craniofacial bone defects requiring high accuracy. The scaffold, constructed using DLP-based 3D printing, showed excellent properties and effectively promoted bone regeneration both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Xinchen Wu, Olivia Gauntlett, Tengfei Zhang, Sanika Suvarnapathaki, Colleen McCarthy, Bin Wu, Gulden Camci-Unal
Summary: Scaffold-based bone regeneration using eggshell microparticles in protein-derived hydrogels significantly promotes bone regeneration by inducing osteogenic differentiation and supporting tissue infiltration over a 12-week period.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Lingjun Wang, Jiannan Mao, Feng Cai, Jincheng Tang, Kun Xi, Yu Feng, Yichang Xu, Xiao Liang, Yong Gu, Liang Chen
Summary: This study successfully guided the regeneration of critical-size bone defects on long bones and restored the natural bone anatomical structure through the construction of a scaffold with heterogeneous biomimetic structure.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Esther Wehrle, Duncan C. Tourolle ne Betts, Gisela A. Kuhn, Erica Floreani, Malavika H. Nambiar, Bryant J. Schroeder, Sandra Hofmann, Ralph Mueller
Summary: This study examined the therapeutic effects of collagen and BMP-2 treatment on bone defects of different sizes using micro-CT imaging. It was found that BMP-2 significantly accelerated the healing process and increased the union rate, indicating its potential for improving bone regeneration outcomes.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Nikola Stokovic, Natalia Ivanjko, Igor Erjavec, Anita Breski, Mihaela Peric, Slobodan Vukicevic
Summary: The study found that adding Zoledronate (ZOL) to autologous bone graft substitute (ABGS) enhances the quality and quantity of newly formed bone, and also prolongs its longevity to a certain extent.
Article
Engineering, Environmental
Jun Li, Yixiang Lai, Mingxin Li, Xingyu Chen, Mi Zhou, Wenzhao Wang, Jiajie Li, Weitong Cui, Geru Zhang, Kefeng Wang, Lei Liu, Yunfeng Lin
Summary: This study proposes a novel approach combining tetrahedral DNA nanostructure (TDN) with 3D bioprinting to treat infected bone defects. The results demonstrate that a bioprinted scaffold loaded with TDN and antibiotics shows promising efficacy in treating infected bone defects in a rat model. This research has significant implications for the treatment of infected bone defects.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Engineering, Biomedical
Pingping Han, Guillermo A. Gomez, Georg N. Duda, Saso Ivanovski, Patrina S. P. Poh
Summary: The dynamics of cell mechanics and epigenetic signatures play a crucial role in cell behavior and fate, affecting the outcomes of tissue regeneration. While 2D geometric substrates have been widely used to study cell mechanics in response to the extracellular microenvironment, there is limited knowledge about cell mechanobiology and epigenetics in 3D biomaterial matrices. This review explores the potential of additive manufacturing for incorporating multi length-scale geometry features on scaffolds, and discusses how scaffold geometry influences cell and nuclear mechanosensing and epigenetic modifications, ultimately impacting tissue regeneration outcomes.
ACTA BIOMATERIALIA
(2023)
Article
Orthopedics
Philippe Moewis, Rene Kaiser, Adam Trepczynski, Christoph von Tycowicz, Leonie Krahl, Ansgar Ilg, Johannes Holz, Georg N. Duda
Summary: This study aimed to analyze knee kinematics in patients with patient-specific episealer implants and found that these implants led to knee kinematics similar to healthy knees, with a medial pivot and high degree of femoral rollback during flexion. However, compared to healthy knees, the episealer implants showed higher femoral rollback and axial external rotation, suggesting possible post-operative muscle weakness and insufficient stabilization at high flexion.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Article
Endocrinology & Metabolism
Tengteng Tang, William Landis, Stephane Blouin, Luca Bertinetti, Markus A. Hartmann, Andrea Berzlanovich, Richard Weinkamer, Wolfgang Wagermaier, Peter Fratzl
Summary: The spatial distribution of mineralization density is vital for bone growth and remodeling processes, while alterations are linked to diseases. In addition to the known lacunocanalicular network, a new nanochannel network has been discovered in the mineralized extracellular matrix of human femoral cortical bone. This network has a greater volume fraction and surface area, potentially allowing for ion and small molecule transport.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Obstetrics & Gynecology
Alexander Paping, Clara Basler, Kerstin Melchior, Loreen Ehrlich, Mario Thiele, Georg N. Duda, Wolfgang Henrich, Thorsten Braun
Summary: The study aimed to evaluate the reliability of intraoperative ultrasound in identifying histologically confirmed uterine scar tissue. The results showed that intraoperative ultrasound was more accurate in identifying scar tissue compared to preoperative ultrasound. This is important for studying uterine wound healing and preventing uterine wall rupture.
JOURNAL OF PERINATAL MEDICINE
(2023)
Review
Cardiac & Cardiovascular Systems
Maurizio Pesce, Georg N. Duda, Giancarlo Forte, Henrique Girao, Angel Raya, Pere Roca-Cusachs, Joost P. G. Sluijter, Carsten Tschope, Sophie Van Linthout
Summary: A growing body of evidence suggests that the mechanical functions of cardiac fibroblasts, as a necessary component, play an active role in myocardial growth and homeostasis. This review describes cell mechanosensation as a regulator of cardiac maturation and disease, and provides evidence that changes in the mechanical properties of the myocardium can occur due to disease-induced remodeling of the cardiac extracellular matrix.
NATURE REVIEWS CARDIOLOGY
(2023)
Review
Rheumatology
Georg N. N. Duda, Sven Geissler, Sara Checa, Serafeim Tsitsilonis, Ansgar Petersen, Katharina Schmidt-Bleek
Summary: Bone has the ability to regenerate and restore its prior mechanical function without scarring, even in challenging conditions. However, many bone injuries still heal unsatisfactorily. The mechanisms leading to impaired healing involve excessive immune reactions and mechanical conditions that affect the regulation of scar-free healing. This review discusses the complex processes involved in bone regeneration, particularly the early healing phase, and the importance of controlling inflammation and tissue organization for successful regeneration. Various therapies, including immunotherapy and mechanobiology-based approaches, are being investigated for promoting bone regeneration.
NATURE REVIEWS RHEUMATOLOGY
(2023)
Article
Engineering, Biomedical
Agathe Bessot, Jennifer Gunter, David Waugh, Judith A. Clements, Dietmar W. Hutmacher, Jacqui McGovern, Nathalie Bock
Summary: Increasing evidence highlights the important role of bone marrow adipocytes in prostate cancer bone metastases. However, lack of relevant models has hindered the understanding of human BM-adipocyte effects in this microenvironment. This study combines tunable GelMA-based hydrogels with biomimetic culture of human cells to engineer human bone tumor microenvironments containing BM-adipocytes. In vitro culture of human osteoprogenitors, adipocytes, and PCa cells in GelMA hydrogels leads to the formation of mineralized, adipose, and PCa tumor 3D microtissues, respectively. The stiffness of hydrogels regulates osteoblast mineralization and tumor spheroid formation, while coculture with tumor cells induces morphological changes and delipidation in BM-adipocytes. The in vivo model successfully forms a humanized fatty bone microenvironment and demonstrates that adipocytes support early bone tumor growth. This advanced platform combines natural aspects of the microenvironment with tunable properties for bone tumor research.
ADVANCED HEALTHCARE MATERIALS
(2023)
Editorial Material
Chemistry, Multidisciplinary
Peter Fratzl
Article
Chemistry, Physical
Laura Zorzetto, Ernesto Scoppola, Emeline Raguin, Kerstin G. Blank, Peter Fratzl, Cecile M. Bidan
Summary: Biofilms form when bacteria colonize a surface and produce extracellular matrix components, including mineral particles such as calcium phosphate. Understanding the mineralization mechanisms in biofilms is crucial for disease prevention and the development of biofilm-based living composites.
CHEMISTRY OF MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Emeline Raguin, Richard Weinkamer, Clemens Schmitt, Luca Curcuraci, Peter Fratzl
Summary: During skeletal development, the transport of large amounts of calcium for bone growth and mineralization at low concentration is a major logistical challenge. Using cryo-FIB/SEM imaging, calcium-rich intracellular vesicles were observed in forming bone tissue of a chick embryo femur. Counting and measuring these vesicles revealed that active transport through the cellular network is involved in calcium logistics, along with calcium-binding proteins and blood flow in the vasculature.
Article
Pharmacology & Pharmacy
Tara Shabab, Onur Bas, Bronwin L. Dargaville, Akhilandeshwari Ravichandran, Phong A. Tran, Dietmar W. Hutmacher
Summary: This study combines melt-extrusion based 3D printing and porogen leaching to develop tunable multiphasic scaffolds for dental tissue regeneration. The scaffolds are highly adjustable in terms of mechanical properties, degradation kinetics, and surface morphology. The research also demonstrates improved cell attachment, proliferation, and extracellular matrix production on these multiscale scaffolds compared to single-scale counterparts, indicating their potential for enhanced tissue regeneration. Furthermore, the study explores the use of these scaffolds as drug delivery devices and shows that sustained drug release can be achieved. These findings strongly support further development of these scaffolds for dental tissue regeneration applications.
Review
Cell Biology
Lisa-Marie Burkhardt, Christian H. Bucher, Julia Loeffler, Charlotte Rinne, Georg N. Duda, Sven Geissler, Tim J. Schulz, Katharina Schmidt-Bleek
Summary: Patients with musculoskeletal diseases face a lower quality of life and higher medical expenses. The interaction between immune cells and mesenchymal stromal cells plays a crucial role in bone regeneration. Excessive accumulation of adipocytes has been linked to low-grade inflammation and impaired bone regeneration. This review discusses the features of bone marrow adipocytes and explores the potential therapeutic approach of targeting peroxisome proliferator-activated receptor gamma (PPARG) to enhance bone regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Endocrinology & Metabolism
Adalbert Raimann, Barbara M. Misof, Peter Fratzl, Nadja Fratzl-Zelman
Summary: This review provides a concise summary of the complex interplay between bone modeling and remodeling during skeletal growth and highlights the complexity of bone tissue characteristics in children with bone disorders.
CURRENT OSTEOPOROSIS REPORTS
(2023)
Article
Engineering, Manufacturing
Buddhi Herath, Markus Laubach, Sinduja Suresh, Beat Schmutz, J. Paige Little, Prasad K. D. V. Yarlagadda, Dietmar W. Hutmacher, Marie-Luise Wille
Summary: A modular design workflow was developed to facilitate the efficient design and manufacture of patient-specific scaffolds for scaffold-guided bone regeneration (SGBR). The workflow allows for semi-automatic filling of defect cavities, patient specificity, ideal surgical scaffold insertion, and generation of scaffolds based on various pore architectures. The real-time feedback from surgeons shortens the time required to finalize a patient-specific scaffold design.
VIRTUAL AND PHYSICAL PROTOTYPING
(2023)
Article
Rheumatology
Marcel Niemann, Melanie Ort, Luis Lauterbach, Mathias Streitz, Andreas Wilhelm, Gerald Gruetz, Florian N. Fleckenstein, Frank Graef, Antje Blankenstein, Simon Reinke, Ulrich Stoeckle, Carsten Perka, Georg N. Duda, Sven Geissler, Tobias Winkler, Tazio Maleitzke
Summary: This study compared the leukocyte and cytokine distributions of different PRP products and assessed the effect of pro-inflammatory cytokines on chondrocytes. The results showed that all three PRP products contained elevated levels of leukocytes, and the nSTRIDE APS product had significantly higher concentrations of the pro-inflammatory cytokines IFN-gamma and TNF-alpha. Furthermore, treatment with IFN-gamma and TNF-alpha negatively affected chondrocyte proliferation and cartilage formation. These findings suggest that both the individual's immune profile and the concentration method impact the composition of PRP products.
ARTHRITIS RESEARCH & THERAPY
(2023)