Article
Chemistry, Multidisciplinary
Sharath Narayana Iyengar, Jiri Dietvorst, Amparo Ferrer-Vilanova, Gonzalo Guirado, Xavier Munoz-Berbel, Aman Russom
Summary: Sepsis is a serious bloodstream infection that compromises the host's immunity. This study introduces a rapid detection method for bacteria in blood, combining selective cell lysis and a sensitive colorimetric approach to detect as low as 10(3) CFU/mL bacteria in less than 5 hours.
Article
Biochemical Research Methods
Akira Imakiire, Sakiko Soutome, Yuichi Nakamura, Moeko Nakamatsu, Keiichiro Miura, Yuki Sakamoto, Masahiro Umeda
Summary: Aspiration pneumonia is a common complication in perioperative and older patients, and various oral care methods are used to prevent it. This study developed a novel method called Delayed real-time PCR (DR-PCR) to quantitatively measure viable bacteria from mixed samples of viable and dead bacteria. The method showed that povidone-iodine mouthwashing reduced the number of viable bacteria to approximately 1/4 of the initial count. Despite being slightly more time consuming than real-time PCR, DR-PCR is effective for studying changes in bacterial counts before and after oral care.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Chemistry, Analytical
Kamayani Vajpayee, Preet Desai, Prakshal B. Parekh, Hrishita C. Sitwala, Kanishkaa Suri, Hirak Ranjan Dashd, Ritesh K. Shukla
Summary: Detection of body fluid is crucial in forensic investigation, with blood being the most frequently encountered evidence at crime scenes. However, current methods using reagents for blood detection often show toxic behavior and false-positive results. This study aims to develop a state-of-the-art methodology using a fluorescein-derived dye, DCFDA, for preliminary blood detection and screening, without causing DNA damage or false-positive reactions.
MICROCHEMICAL JOURNAL
(2023)
Review
Biochemical Research Methods
Martijn Diender, Ivette Parera Olm, Diana Z. Sousa
Summary: Synthetic microbial co-cultivation is an increasingly explored approach in biotechnology, which enhances the metabolic potential and process capabilities of microbes. The research findings suggest that co-cultivation can reduce undesired side reactions, promote microbial interactions, and open up new possibilities in areas like waste conversion.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Mengtao Chen, Xinyue Lan, Longjiao Zhu, Ping Ru, Wentao Xu, Haiyan Liu
Summary: In order to ensure food safety, it is necessary to detect viable bacteria in a short period of time. Traditional bacterial culture methods are time-consuming, so PCR mediated nucleic acid molecular recognition technologies have been developed to quickly and accurately detect foodborne pathogens.
Article
Microbiology
Zihang Shi, Xiefei Li, Xiankang Fan, Jue Xu, Qing Liu, Zhen Wu, Daodong Pan
Summary: The number of viable lactic acid bacteria (LAB) is crucial for evaluating the quality of fermented milk. This study used the PMA-qPCR method to rapidly and quantitatively detect the number of viable bacteria of each LAB species in fermented milk. By analyzing the pheS gene, it was found that pheS gene resolved species identities better and was thus selected to design specific primers and probes. The results showed that PMA-qPCR detected significantly lower numbers of viable bacteria compared to qPCR, indicating the effectiveness of PMA in inhibiting amplification of DNA from dead cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Multidisciplinary
Yonglin Wu, Dongxu Gao, Yinfeng Fang, Xue Xu, Hongwei Gao, Zhaojie Ju
Summary: This paper proposes an improved target detection algorithm, SDE-YOLO, based on the YOLOv5s framework, to address the low detection accuracy, misdetection, and leakage in blood cell detection caused by existing single-stage and two-stage detection algorithms. The SDE-YOLO algorithm achieves high accuracy and performance in detecting blood cells, surpassing other algorithms such as SSD, YOLOv4, and YOLOv5s.
Article
Engineering, Biomedical
Angeles I. Rodriguez-Villarreal, Laura Ortega Tana, Joan Cid, Aurora Hernandez-Machado, Tomas Alarcon, Pere Miribel-Catala, Jordi Colomer-Farrarons
Summary: This study presents a handheld microrheometer fabricated using microtechnology, capable of measuring the viscosity of various fluids with high accuracy. The device can provide non-linear viscosity measurements of blood samples in less than 30 seconds, making it suitable for a wide range of biomedical applications.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2021)
Article
Food Science & Technology
Xiankang Fan, Xiefei Li, Tao Zhang, Jue Xu, Zihang Shi, Zhen Wu, Jihuan Wu, Daodong Pan, Lihui Du
Summary: The study focused on using the tuf gene for qualitative and quantitative detection of six LAB strains in yogurt, demonstrating that this method was closer to the true level of LAB in yogurt compared to traditional methods, and could calculate the distribution of live and dead bacteria.
Article
Chemistry, Analytical
Niu Feng, Shu Wang, Luyu Wei, Qinyu Wang, Xinrui Cheng, Peng Lu, Xuewen Peng, Xufeng Wang, Chen Zhan, Yiming Dong, Yiping Chen
Summary: In this study, a sensing method called SMART was developed for rapid, sensitive, and multiplex profiling of foodborne pathogens using artificial intelligence transcoding. The method utilizes programmable polystyrene microspheres to encode different pathogens and generate visible signals under conventional microscopy. A customized artificial intelligence-computer vision was trained to decode the microspheres' intrinsic properties and reveal the numbers and types of pathogens. The assay enables the rapid and simultaneous detection of multiple bacteria without DNA amplification, and shows strong consistency with standard microbiologic and genotypic methods. Phage-guided targeting is adopted to discriminate between live and dead bacteria.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Yuichi Ogawa, Shojiro Kikuchi, Yoshihisa Yamashige, Keiichiro Shiraga, Takeshi Mitsunaka
Summary: This study monitored the growth of Escherichia coli using a CMOS near-field sensor array, successfully distinguishing between viable and heat-treated dead E. coli, and observing the suppression of growth by streptomycin. The results suggest that viable E. coli can be rapidly confirmed by changes in permittivity near the sensor surface caused by displacement of media by the bacteria.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Food Science & Technology
Manyun Yang, Xiaobo Liu, Yaguang Luo, Arne J. Pearlstein, Shilong Wang, Hayden Dillow, Kevin Reed, Zhen Jia, Arnav Sharma, Bin Zhou, Dan Pearlstein, Hengyong Yu, Boce Zhang
Summary: The system developed integrates paper chromogenic arrays (PCAs) and machine learning to automatically recognize PCA patterns on multiplexed viable pathogens with strain-level specificity. This approach has the potential to advance non-destructive pathogen detection and identification on food.
Article
Chemistry, Multidisciplinary
Massimo Rippa, Riccardo Castagna, Domenico Sagnelli, Ambra Vestri, Giorgia Borriello, Giovanna Fusco, Jun Zhou, Lucia Petti
Summary: This study discovered a new strategy for detecting Brucella using SERS nano-biosensing technology, which can identify the pathogen in milk and has the potential for on-site and in-food detection.
Article
Food Science & Technology
Guoyang Xie, Donggen Zhou, Guojing Zhao, Xiaoyan Feng, Zoraida P. Aguilar, Hengyi Xu
Summary: The study proposed a method for rapid and real-time detection of viable S. aureus in milk, combining PMAxx dye and RAA technology. It can accurately detect S. aureus in a short time, which is of great significance for effective monitoring of food safety.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Energy & Fuels
Yljon Seferi, Steven M. Blair, Christian Mester, Brian G. Stewart
Summary: Electric arcing caused by contact interruption between the pantograph and the overhead contact line in electrified railway networks is a significant issue. Early detection of arcing events can reduce system wear and improve pantograph performance. The method of quantifying the rate of change of current signal phase can effectively detect and localize electric arcing.
