Article
Chemistry, Physical
Zachary Geeraerts, Olivia R. Stiller, Gudrun S. Lukat-Rodgers, Kenton R. Rodgers
Summary: The heme-based chlorite dismutases can catalyze the decomposition of both chlorite and bromite. The activity and product formation of the chlorite dismutase are pH-dependent, suggesting the involvement of proton-coupled electron transfer.
Article
Chemistry, Physical
Zachary Geeraerts, Olivia R. Stiller, Gudrun S. Lukat-Rodgers, Kenton R. Rodgers
Summary: The heme-based chlorite dismutases can also catalyze the decomposition of bromite. The formation of Cpd II and center dot ClO2 is observed in the reaction mixture, and the rate of proton transfer plays a critical role in the oxidation of an amino acid. These findings provide insights into the catalytic activity of chlorite dismutases and their potential applications.
Article
Cardiac & Cardiovascular Systems
Sergey I. Dikalov, Anna E. Dikalova, Igor A. Kirilyuk
Summary: Superoxide radical plays an important role in redox cell signaling and physiological processes. However, studying superoxide in biological systems faces challenges such as its short lifetime and lack of site-specific detection. In this study, a new method using N-15-CAT1H spin probe was developed for high sensitivity and site-specific detection of extracellular superoxide. This provides a new approach to study important sources of oxidative stress in cardiovascular diseases.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Kranti A. Mapuskar, Emily J. Steinbach, Amira Zaher, Dennis P. Riley, Robert A. Beardsley, Jeffery L. Keene, Jon T. Holmlund, Carryn M. Anderson, Diana Zepeda-Orozco, John M. Buatti, Douglas R. Spitz, Bryan G. Allen
Summary: Cisplatin is a widely used chemotherapy agent with potent anti-tumor activity, but it can cause renal injuries, leading to the need for intervention strategies to reduce kidney damage. In addition to DNA cross-linking, cisplatin affects mitochondrial metabolism, resulting in reactive oxygen species production and renal damage. Mitochondrial superoxide dismutase mimetics show potential in preventing platinum-associated nephrotoxicity.
Article
Biochemistry & Molecular Biology
Jose Teixeira, Farhan Basit, Peter H. G. M. Willems, Jori A. Wagenaars, Els van de Westerlo, Ricardo Amorim, Fernando Cagide, Sofia Benfeito, Catarina Oliveira, Fernanda Borges, Paulo J. Oliveira, Werner J. H. Koopman
Summary: The novel mitochondria-targeted variants of gallic and caffeic acid were found to increase ROS levels, stimulate NRF2 expression, and increase SOD2 and GSH levels.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Chemistry, Inorganic & Nuclear
Molly M. Lockart, Kyle C. Edwards, John B. Vincent, Brad S. Pierce
Summary: The study detected an EPR signal for Mn(III) bound to transferrin, suggesting the six-coordinate nature of the Mn(III) centers in an elongated tetragonal environment. Incorporation of Mn(III) within the Tf active site did not significantly alter the coordination number or active site geometry compared to native Fe(III)(2)-Tf. The parallel mode EPR signal for Mn(III)(2)-Tf could be valuable for future studies on the physiological relevance of Mn(III)(2)-Tf.
Article
Pharmacology & Pharmacy
Xinxin Shao, Mengzhu Zhang, Yingxuan Chen, Siyu Sun, Shengcai Yang, Quanshun Li
Summary: Aging is a process driven by reactive oxygen species (ROS), and superoxide dismutase (SOD) can scavenge ROS to extend lifespan. However, the instability and impermeability of native SOD limit its application. In this study, SOD was encapsulated into exosomes to obtain SOD@EXO, which significantly extended the lifespan of C. elegans and improved resistance against stress conditions.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Janae D. Sweeney, Marija Debeljak, Stacy Riel, Ana Cecilia Millena, James R. Eshleman, Channing J. Paller, Valerie Odero-Marah
Summary: The research showed that SOD2 expression did not correlate with tumor aggressiveness or SOD2 genotype in prostate cancer. However, the Ala-SOD2 allele may promote EMT, leading to increased cell migration. The antioxidant MSKE could inhibit EMT mediated by Ala-SOD2 SNP, indicating promising therapeutic potential for halting prostate cancer progression.
Review
Biochemistry & Molecular Biology
Brianne R. O'Leary, Rory S. Carroll, Garett J. Steers, Jennifer Hrabe, Frederick E. Domann, Joseph J. Cullen
Summary: Reactive oxygen species are essential components in cell signaling and immune responses, but an imbalance can lead to oxidative stress. Cancer cells show increased oxidative stress and decreased expression of extracellular superoxide dismutase, an antioxidant enzyme that plays a role in maintaining redox balance and protecting against oxidative damage. Understanding the role of EcSOD in cancer could have potential implications for detection, prognosis, and treatment of malignancies.
Article
Biochemistry & Molecular Biology
Hoi-Shan Wong, Martin D. Brand, Vojtech Mezera, Pratiksha Dighe, Simon Melov, Akos A. Gerencser, Ramzi F. Sweis, Marina Pliushchev, Zhi Wang, Tim Esbenshade, Bryan McKibben, Stephan Riedmaier, Martin D. Brand
Summary: The study demonstrated the effectiveness of novel variants of S1QELs in vivo, showing that mitochondrial site I-Q plays a significant role in driving pathology in Sod2(-/-) mice, while site IIIQo does not significantly contribute to mitochondrial superoxide production in these mice. Treatment with S1QEL352 and S1QEL712 improved cardiac and hepatic pathologies in Sod2(-/-) mice, confirming the importance of superoxide production from mitochondrial site I-Q in hepatic steatosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Veterinary Sciences
Ange Mouithys-Mickalad, Nazare Storms, Thierry Franck, Justine Ceusters, Geoffroy de la Rebiere de Pouyade, Ginette Deby-Dupont, Didier Serteyn
Summary: Juglone inhibits equine laminitis by modulating neutrophil activation, and it also suppresses neutrophil degranulation and the peroxidase activity of MPO.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Chemistry, Physical
Sergei O. Liubimovskii, Leila Yu Ustynyuk, Alexander N. Tikhonov
Summary: Tiron is commonly used as a probe for detection of superoxide radicals. The interaction of Tiron with superoxide radicals dissolved in water solutions was studied using density functional theory (DFT) method. The reactions of Tiron with superoxide radicals are influenced by hydronium ions, leading to the formation of Tiron semiquinone radicals.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Cell Biology
Ting-Yu Chin, Che-Chuan Wang, Kuo-Hsing Ma, Chia-Wei Kuo, Ming-Kuan Hu, Sheau-Huei Chueh
Summary: The study showed that DPMQ protects NG108-15 cells against oxidative stress by stabilizing DJ-1 in a Cu2+-dependent manner, leading to SOD1 activation and Nrf2 nuclear translocation, which alleviates cellular oxidative stress.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Chemistry, Applied
Abhishek Sahu, Kiyoon Min, Sae Hyun Jeon, Kiyoon Kwon, Giyoong Tae
Summary: Researchers developed a highly biocompatible and biodegradable organic-based nanozyme system by conjugating hemin to heparin or chitosan. The heparin-hemin nanostructure showed smaller size, higher stability, and superior superoxide dismutase and catalase activities compared to chitosan-hemin and free hemin. In addition, heparin-hemin demonstrated better cell protection against reactive oxygen species and showed therapeutic effects on an acute kidney injury model.
CARBOHYDRATE POLYMERS
(2023)
Review
Neurosciences
Inna Gertsenshteyn, Mihai Giurcanu, Peter Vaupel, Howard Halpern
Summary: Measuring molecular oxygen levels in vivo is crucial for understanding the effects of hypoxia in normal tissues and malignant tumors. This article explores various PO2 measurements and imaging techniques, with a focus on the validation of treating hypoxic tumors with a threshold of PO2 <= 10 Torr using EPR imaging. These techniques have significant implications for studying the impact of hypoxia on normal tissues and malignant tumors.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)