Article
Medical Laboratory Technology
Adrian Post, Erwin Garcia, Irina Shalaurova, Steven P. Matyus, Jessica M. Gonzalez-Delgado, Caecilia S. E. Doorenbos, Yvonne van der Veen, Svati H. Shah, William E. Kraus, Daan Kremer, Tim J. Knobbe, Stephan J. L. Bakker, Robin P. F. Dullaart, Margery A. Connelly
Summary: The study found that impaired creatine homeostasis may contribute to fatigue in kidney transplant recipients. A high-throughput NMR assay was developed and validated to measure circulating creatine and creatinine concentrations in these patients. Higher plasma creatine concentration and estimated intramuscular creatine concentration were associated with lower fatigue scores and fewer motivation problems.
CLINICA CHIMICA ACTA
(2023)
Article
Transplantation
Yishan Dong, Stephen M. Silver, Richard H. Sterns
Summary: Spot determinations of urine creatinine concentration are commonly used to estimate 24-hour urine excretion rates, but these estimates can be inaccurate if the serum creatinine concentration is changing or if there are extreme variations in muscle mass. Several equations have been proposed for estimating urine volume based on urine creatinine concentrations, but none of them are accurate in all situations.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2023)
Article
Veterinary Sciences
Sydney Banton, Ulrike Braun, E. James Squires, Anna K. Shoveller
Summary: This study investigated the plasma creatine and creatinine response in dogs fed a commercial diet top-dressed with creatine, carnitine, and choline. The results showed that feeding the combination of these compounds increased plasma creatine levels for up to 6 hours post-meal, which could have implications for energy metabolism. Therefore, this should be taken into consideration when using creatinine as a diagnostic tool in dogs.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Hao Zhang, Zhilin Ouyang, Nannan Zhao, Shuangyan Han, Suiping Zheng
Summary: In this study, the AmtR regulon in C. glutamicum ATCC 14067 was identified using ChIP-seq, revealing new operons related to creatine and creatinine degradation. The negative regulation of transcription and expression of these genes by AmtR was confirmed. Additionally, it was found that C. glutamicum ATCC 14067 can utilize creatine or creatinine as a sole nitrogen source.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Sergej M. Ostojic
Summary: Based on 2017-2018 NHANES data, this study found no significant association between dietary creatine intake and kidney dysfunction in 2,955 U.S adults.
FOOD SCIENCE & NUTRITION
(2021)
Article
Chemistry, Multidisciplinary
Seydou Sanogo, Paolo Silimbani, Raffaella Gaggeri, Carla Masini
Summary: The study aimed to develop and validate a single chromatographic method for determining the chemical stability of 5 chemotherapy drugs. The method demonstrated excellent linearity, sensitivity, precision, and accuracy, as well as robustness in analyzing drug stability, making it an effective analytical approach.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Olga Begou, Kathrin Weber, Bibiana Beckmann, Dimitrios Tsikas
Summary: Creatinine is a useful endogenous biochemical parameter for correcting the urinary excretion rate of various substances. Derivatization methods for creatinine and creatine allow for accurate measurement using GC-MS.
Article
Polymer Science
Min Gi Cho, Seonghoon Hyeong, Kyung Kgi Park, Sung Hyo Chough
Summary: The molecularly imprinted polymer (MIP) for creatinine showed a high specificity with two types of effective binding sites (high and low affinity), while non-imprinted polymer (NIP) had only one type of binding site. The sorption of creatinine was found to be a mono-layered following Langmuir isotherm, while creatine could have multi-layered adsorption of Type V according to IUPAC classification.
Article
Medical Laboratory Technology
Mette B. Jensen, Ingunn Viken, Frederikke Hogh, Katja K. Jacobsen
Summary: This study assessed the quantification of urinary albumin and creatinine using two different analytical platforms and examined the concordance of albumin to creatinine ratio (ACR) stratification. The results showed that differences in urinary albumin results are clinically significant, and there is a risk of incorrectly understanding a patient's kidney disease progression despite high agreement between the ACR results.
CLINICAL BIOCHEMISTRY
(2022)
Article
Environmental Sciences
Xiaocui Qiao, Simin Ge, Chengyou Liu, Lixin Jiao, Xue Li, Xingru Zhao, Tong Qi, Yan Liu
Summary: Microcystins are hepatotoxic secondary metabolites of cyanobacteria, which can be accurately quantified in water using online-SPE UPLC-MS/MS. A method was developed to simultaneously quantify eight different MC congeners in water with high sensitivity and resolution, demonstrating potential for monitoring water contamination levels.
ENVIRONMENTAL SCIENCES EUROPE
(2021)
Article
Medical Laboratory Technology
Gabriel Alvarez Alvarez Menendez, Olaya Amor-Gutierrez, Agustin Costa Garcia, Maria Funes-Menendez, Catuxa Prado, Diego Miguel, Pablo Rodriguez-Gonzalez, Adriana Gonzalez-Gago, J. Ignacio Garcia Alonso
Summary: An electrochemical biosensor was developed for creatinine determination in human blood samples. It utilized a modified dual carbon working electrode with three enzymes and horseradish peroxidase as mediator. The biosensor showed linear response to creatinine concentration in the range of 0.5-15 mg/dL and exhibited long-term stability. Comparison with routine techniques showed no significant differences in creatinine concentrations.
CLINICA CHIMICA ACTA
(2023)
Article
Pharmacology & Pharmacy
Xueqiao Wang, Xinhua Dai, Shiqi Wan, Yu Fan, Lijuan Wu, Huan Xu, Lin Yan, Xingxin Gong, Yamei Li, Yao Luo, Yangjuan Bai, Yi Li
Summary: In this study, a new method combining VAMS and UPLC-MS/MS was developed to simultaneously quantify TAC, MPA, and Cre concentrations in renal transplant recipients. The method was successfully validated and showed higher patient satisfaction compared to dried blood spots.
Article
Chemistry, Analytical
Teenu Sharma, Rajneet Kaur Khurana, Bonita Borges, Ranjot Kaur, O. P. Katare, Bhupinder Singh
Summary: Plant bioactives have shown therapeutic potential in cancer management, with combinations like sorafenib and chrysin exhibiting synergistic effects. A validated HPTLC densitometric method was developed for simultaneous analysis of these compounds, showcasing its applicability in drug formulations and biological matrices.
MICROCHEMICAL JOURNAL
(2021)
Article
Engineering, Environmental
Alex H. S. Chik, Jane J. Y. Ho, Nivetha Srikanthan, Hadi Dhiyebi, Mark Servos
Summary: Global evaluations of biomolecular-based methods for monitoring SARS-CoV-2 in wastewater, such as RT-qPCR, are being conducted through interlaboratory comparisons. These evaluations often use a common reference wastewater sample, but split-sample comparisons are also performed to assess the native SARS-CoV-2 genetic signal in the sample. A reproducible procedure is necessary to minimize variability among split wastewater aliquots, especially when a large number of aliquots are required. This study proposes a portable batch reactor design and demonstrates its effectiveness in splitting real wastewater samples.
JOURNAL OF ENVIRONMENTAL ENGINEERING
(2022)
Article
Engineering, Mechanical
Mohamed A. A. Ismail, Edward Balaban, Jens Windelberg
Summary: Flight control electro-mechanical actuators (EMAs) are crucial for the reliability and safety of unmanned aerial vehicles. This study develops a vibration-based fault quantification method for fatigue faults in the ball-screw mechanism of an EMA. The method identifies the ball passing instants through a localized surface defect using vibrational jerk measurements.