Review
Chemistry, Analytical
Guadalupe Garcia-Llatas, Amparo Alegria, Reyes Barbera, Antonio Cilla
Summary: Plant sterols are bioactive compounds found in all vegetable foods, with potential beneficial effects on lowering cholesterol levels. Analytical methods are needed to assess their health benefits and future applications. Current methodologies include extraction and analysis, with mass spectrometry widely used for its sensitivity and selectivity.
MICROCHEMICAL JOURNAL
(2021)
Article
Environmental Sciences
Cuiyun Wei, Zhen Zhou, Ling Wang, Zichun Huang, Yong Liang, Jie Zhang
Summary: This study revealed that exposure to PFOS led to disturbances in fatty acid metabolism in C. elegans, characterized by decreased levels of C18:0, increased levels of C18:3n6, decreased C18:3n6: C18:2n6 ratio, and down-regulation of the FA desaturation gene fat-3. Additionally, exposure disrupted mitochondrial function, leading to reduced ATP synthesis.
Article
Food Science & Technology
Xufang Wu, Fengen Wang, Meiqing Chen, Jiaqi Wang, Yangdong Zhang
Summary: The aim of this research was to establish a method for quantifying 10 short-chain fatty acids (FSCFAs) in raw cow milk. Acidified raw cow milk samples were processed using gas chromatography-mass spectrometry after pretreatment with hydrochloric acid ethanol (0.5%) solution. Except for iso C5:0 and anteiso C5:0 co-elution, the remaining eight FSCFAs were effectively separated by chromatography. The methodological validation showed satisfactory linear relationships, low limits of quantification, good recoveries, and precision. Our study provides a valuable method for future quality and safety control of raw milk and nutritional studies.
Article
Biochemical Research Methods
Mateusz Dembowski, John E. Rowley, Kevin Boland, Janelle Droessler, David A. Hathcoat, Alexandria Marchi, George S. Goff, Iain May
Summary: This study evaluates the application of High-Speed Counter-Current Chromatography (HSCCC) in the separation of non-radioactive rare earth elements (REEs). The results demonstrate the feasibility and effectiveness of HSCCC in separating a subset of REEs, providing potential applications in radioelement separation chemistry.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Agriculture, Dairy & Animal Science
Fengen Wang, Meiqing Chen, Runbo Luo, Guoxin Huang, Xufang Wu, Nan Zheng, Yangdong Zhang, Jiaqi Wang
Summary: A high-throughput gas chromatography-mass spectrometry method was developed to determine milk fatty acid compositions. The fatty acid profiles of different types of milk were analyzed, and the results showed significant differences among different milk samples. Goat milk and camel milk were considered as potential functional foods.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Analytical
Sophia A. Schreckenbach, Denina Simmons, Adam Ladak, Lauren Mullin, Derek C. G. Muir, Myrna J. Simpson, Karl J. Jobst
Summary: This study developed a suspect screening method based on industrial compounds with bioaccumulation potential. Results showed that SQDIA has a significantly lower false-positive rate compared to standard DIA. Through suspect screening and nontargeted screening, multiple pollutants were identified, with some compounds confirmed through standards and structural analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Zahir Shah, Syed Lal Badshah, Arshad Iqbal, Zamarud Shah, Abdul-Hamid Emwas, Mariusz Jaremko
Summary: Freshwater macroalgae were found to contain important secondary metabolites with medicinal properties, which can be used as biopesticides, plant growth enhancers, and remedies for diseases in farm animals. The extracts also contain essential oils that can be utilized in various industrial products.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2022)
Review
Medical Laboratory Technology
Kenichi Tamama
Summary: Drugs of abuse testing is commonly used in clinical and forensic settings, with urine being the preferred specimen type but other emerging types include saliva, sweat, hair, and meconium. Liquid chromatography-tandem mass spectrometry (LC-tandem-MS) has become the new gold standard for confirmatory drug testing due to its ability to analyze a wider range of analytes compared to gas chromatography-mass spectrometry (GC-MS). Technical advancements such as high-resolution mass spectrometry and miniature ambient ionization mass spectrometry have improved the speed and efficiency of drugs of abuse testing.
CLINICA CHIMICA ACTA
(2021)
Review
Spectroscopy
Maria Khalikova, Jakub Jires, Ondrej Horacek, Michal Dousa, Radim Kucera, Lucie Novakova
Summary: The role of mass spectrometry (MS) in pharmaceutical analysis varies depending on the stage of drug development and the type of pharmaceutical. While it dominates in drug discovery and development, MS plays a minor and indispensable role in routine quality control. The analysis of biopharmaceuticals requires a larger panel of methods and extensive use of MS for quality control.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Pharmacology & Pharmacy
Vijay Kumar Shankar, Mei Wang, Srinivas Ajjarapu, Praveen Kolimi, Bharathi Avula, Reena Murthy, Ikhlas Khan, Sathyanarayana Narasimha Murthy
Summary: A validated GC/SIM-MS method for quantifying docosanol in biological samples was developed and confirmed to be sensitive, specific, and suitable for quality control analysis of docosanol.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Multidisciplinary Sciences
Cara Olivier, Bianca Allen, Laneke Luies
Summary: This study optimized a urine preparation procedure for non-targeted metabolomics using GC-MS. The direct analysis method showed superior repeatability and higher metabolome coverage compared to the organic acid methods. The findings contribute to the advancement of urine metabolomics research.
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
Liyuan Wang, Zhengyan Hu, Jing Chen, Tianjiao Wang, Pinggu Wu, Ying Ying
Summary: This study developed a method to simultaneously detect a dozen preservatives in pastry using gas chromatography-mass spectrometry. The preservatives showed good linearity within the range of 1.0-50 μg/mL, with detection limits of 0.04-2.00 mg/kg and quantification limits of 0.12-6.67 mg/kg. The method is suitable for the determination of 12 preservatives in pastry.
Article
Oncology
Alicja Pakiet, Agata Jedrzejewska, Katarzyna Duzowska, Alina Waclawska, Patrycja Jablonska, Jacek Zielinski, Adriana Mika, Tomasz Sledzinski, Ewa Slominska
Summary: The aim of this study was to examine the serum fatty acids (FAs) profiles in breast cancer survivors to assess if the FA levels normalize. After treatment for breast cancer, the patients' serum FA profile differs from the profile before treatment and from controls, especially 12 months after treatment. Some changes may reflect lifestyle changes in breast cancer survivors and have an impact on the risk of recurrence.
Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Petar Ristivojevic, Vesna Jovanovic, Dusanka Milojkovic Opsenica, Jihae Park, Judith M. Rollinger, Tanja Cirkovic Velickovic
Summary: A novel high-throughput methodological approach was developed for tracing compounds with radical scavenging and antimicrobial activities in brown seaweeds. Major components identified with these activities include stearidonic, eicosapentaenoic, and arachidonic acids. This method allows fast identification and quantification of bioactive compounds in multicomponent biological samples.
Article
Chemistry, Applied
Klaus-J. Appenroth, K. Sowjanya Sree, Volker Bohm, Simon Hammann, Walter Vetter, Matthias Leiterer, Gerhard Jahreis
Article
Agriculture, Multidisciplinary
Andrea Irias-Mata, Wolfgang Stuetz, Nadine Sus, Simon Hammann, Katrin Gralla, Aracelly Cordero-Solano, Walter Vetter, Jan Frank
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2017)
Review
Biochemistry & Molecular Biology
Enrico Cappellini, Ana Prohaska, Fernando Racimo, Frido Welker, Mikkel Winther Pedersen, Morten E. Allentoft, Peter de Barros Damgaard, Petra Gutenbrunner, Julie Dunne, Simon Hammann, Melanie Roffet-Salque, Melissa Ilardo, J. Victor Moreno-Mayar, Yucheng Wang, Martin Sikora, Lasse Vinner, Juergen Cox, Richard P. Evershed, Eske Willerslev
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 87
(2018)
Article
Chemistry, Applied
Marco Mueller, Simon Hammann, Walter Vetter
Article
Anthropology
Simon Hammann, Lucy J. E. Cramp
JOURNAL OF ARCHAEOLOGICAL SCIENCE
(2018)
Article
Chemistry, Organic
Simon Hammann, Lucy J. E. Cramp, Mathilda Whittle, Richard P. Evershed
TETRAHEDRON LETTERS
(2018)
Article
Biochemical Research Methods
Walter Vetter, Marco Mueller, Katrin Sommer, Markus Schroeder, Simon Hammann
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Chemistry, Applied
Simon Hammann, Ansgar Korf, Ian D. Bull, Heiko Hayen, Lucy J. E. Cramp
Article
Multidisciplinary Sciences
Ansgar Korf, Simon Hammann, Robin Schmid, Matti Froning, Heiko Hayen, Lucy J. E. Cramp
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Elizabeth Craig-Atkins, Ben Jervis, Lucy Cramp, Simon Hammann, Alexandra J. Nederbragt, Elizabeth Nicholson, Allie Rae Taylor, Helen Whelton, Richard Madgwick
Article
Multidisciplinary Sciences
Simon Hammann, David J. Scurr, Morgan R. Alexander, Lucy J. E. Cramp
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Melanie J. Miller, Helen L. Whelton, Jillian A. Swift, Sophia Maline, Simon Hammann, Lucy J. E. Cramp, Alexandra McCleary, Geoffrey Taylor, Kirsten Vacca, Fanya Becks, Richard P. Evershed, Christine A. Hastorf
SCIENTIFIC REPORTS
(2020)
Article
Anthropology
Helen L. Whelton, Simon Hammann, Lucy J. E. Cramp, Julie Dunne, Melanie Roffet-Salque, Richard P. Evershed
Summary: Organic residue analysis is a powerful method for extracting information from lipids and other biomolecules preserved in archaeological artifacts. However, there are significant risks associated with applying this technique, due to lack of experience and knowledge, which can lead to the production of data that may lack value or even perpetuate false knowledge.
JOURNAL OF ARCHAEOLOGICAL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Simon Hammann, Rosie R. Bishop, Mike Copper, Duncan Garrow, Caitlin Greenwood, Lanah Hewson, Alison Sheridan, Fraser Sturt, Helen L. Whelton, Lucy J. E. Cramp
Summary: Archaeological research has revealed evidence of cereals (wheat) in Neolithic pottery from Scotland, dating back to around 3600-3300 BCE, suggesting that cereals may have been regularly prepared with dairy products as a milk-based gruel. This study sheds light on culinary traditions among early farming communities.
NATURE COMMUNICATIONS
(2022)
Article
Food Science & Technology
Anne Zartmann, Leon Voelcker, Simon Hammann
Summary: Certain polyunsaturated fatty acids with n-3 double bonds are important nutrients for the human body, and this study analyzed 33 dietary supplements from the German market to determine their lipid compositions. The results showed that triacylglycerols were the dominant lipid species in most samples, but krill oil contained phospholipids. The total lipid profiles and fatty acid analysis varied depending on the lipid source, and there were differences between the labeled and actual quantities of individual fatty acids and vitamin E derivatives in some samples.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)