Review
Pharmacology & Pharmacy
Rahul G. Ingle, Su Zeng, Huidi Jiang, Wei-Jie Fang
Summary: Sample preparation is crucial in bioanalysis, but it faces challenges due to the unique characteristics of different biological matrices. Novel techniques have gained popularity in the past decade for their advantages in accuracy, automation, and convenience.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Multidisciplinary
John M. Kokosa
Summary: Liquid phase microextraction (LPME) techniques are increasingly important in bioanalytical sample preparation due to their versatility, adaptability, sensitivity, and adherence to green analytical chemistry principles. Continuous innovation and improvements in LPME methods provide potential for new applications in bioanalytical sample preparation. Current trends focus on bioanalytical sample preparation and the use of non-traditional green solvents in LPME methods.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Review
Chemistry, Analytical
Joao Raul Belinato, Vinicius Ricardo Acquaro, Luciana Teresa Dias Cappelini, Fabio Augusto
Summary: The interest in bioactive compounds from microorganisms has increased since the first discovery, with microorganisms being a key source of biomolecules with diverse applications. While conventional sample preparation techniques are still widely used, microextraction techniques show promise in overcoming limitations and promoting a deeper understanding of complex systems and the discovery of new molecules.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Douglas M. Sartore, Deyber Vargas A. Medina, Marcio D. Bocelli, Marcela Jordan-Sinisterra, Alvaro J. Santos-Neto, Fernando M. Lancas
Summary: Sample preparation is crucial for the analytical workflow, affecting throughput, costs, error, and contamination. To improve efficiency and minimize impacts, miniaturization and automation are necessary. This review summarizes recent developments in automated microextractions coupled with liquid chromatography, focusing on microextraction automation strategies and their applications in determining small organic molecules in various samples.
JOURNAL OF SEPARATION SCIENCE
(2023)
Review
Plant Sciences
Mohamed A. Salem, Jian You Wang, Salim Al-Babili
Summary: Plants release chemical compounds into the rhizosphere, which plays important roles in inhibiting harmful microorganisms and promoting beneficial microbes, with applications in crop yield and phytoremediation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Stefan Loroch, Dominik Kopczynski, Adriana C. Schneider, Cornelia Schumbrutzki, Ingo Feldmann, Eleftherios Panagiotidis, Yvonne Reinders, Roman Sakson, Fiorella A. Solari, Alicia Vening, Frauke Swieringa, Johan W. M. Heemskerk, Maria Grandoch, Thomas Dandekar, Albert Sickmann
Summary: This study introduces a novel strategy for positive-pressure 96-well filter-aided sample preparation (PF96), which significantly increases throughput and demonstrates high reproducibility. The study confirms the excellent reproducibility and stability of PF96 at the protein level, as well as its compatibility with nanoLC-MS instrumentation. This method has promising applications in biomedical and clinical research.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Runshan Will Jiang, Karol Jaroch, Janusz Pawliszyn
Summary: Improved analytical methods using solid-phase microextraction (SPME) fibers were utilized in this study to monitor changes in endogenous compounds in homogenized and intact ovine lung tissue. The findings highlight the importance of sample preparation in tissue-based metabolomics studies and the unique ability of SPME to perform minimally invasive extractions while providing broad metabolite coverage.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Engineering, Environmental
Shuqin Liu, Yiquan Huang, Jian Liu, Chao Chen, Gangfeng Ouyang
Summary: The study developed a biocompatible SPME fiber for tracking the bioaccumulation and elimination of carbamates and their metabolites in plants. The results showed rapid changes in plant metabolism after exposure to carbamates, which were not fully reversed even after the contaminants were eliminated.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Sylvia P. Nauta, Pascal Huysmans, Gabrielle J. M. Tuijthof, Gert B. Eijkel, Martijn Poeze, Tiffany Porta Siegel, Ron M. A. Heeren
Summary: The developed 3D mass spectrometry scanner enables automated sampling and imaging of uneven topography surfaces with LA-REIMS, allowing for 3D visualization of molecular distributions on these surfaces.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Yu Jing, Varoon Singh, Liqin Chen, Janusz Pawliszyn
Summary: The study introduces a high-throughput method for the simultaneous detection of four OPFR metabolites in urine samples, which is reliable, accurate, and compliant with the guidelines of the Food and Drug Administration.
Article
Parasitology
Halfan S. Ngowo, Alex J. Limwagu, Heather M. Ferguson, Jason Matthiopoulos, Fredros O. Okumu, Luca Nelli
Summary: Improved methods for sampling outdoor-biting mosquitoes are crucial for monitoring vector-borne diseases. This study developed a statistical framework for predicting human-biting rates from different exposure-free alternatives and established a valuable framework for estimating human exposures based on trap catches.
PARASITES & VECTORS
(2022)
Article
Biochemistry & Molecular Biology
Julia M. Malinowska, Taina Palosaari, Jukka Sund, Donatella Carpi, Gavin R. Lloyd, Ralf J. M. Weber, Maurice Whelan, Mark R. Viant
Summary: This study presents an automated sample preparation and analysis workflow for in vitro metabolomics of HepaRG cells using high-resolution mass spectrometry. The workflow demonstrates good repeatability and high sample throughput, which can advance the application of metabolomics in chemical safety.
Article
Chemistry, Analytical
Guanghui Hong, Qingqing Wang, Chao Xu, Xiping Cui, Jie Zhang
Summary: A high-efficiency double-sample sampling system was developed by increasing the sample channels and tee valve on the hardware, as well as adjusting the calibration factor on the software. This system allows for simultaneous introduction of single-element samples, multi-element samples, and combinations of single-element and multi-element samples. It has potential applications in various fields.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Feiyang Li, Ryan Karongo, Despoina Mavridou, Jeannie Horak, Adrian Sievers-Engler, Michael Laemmerhofer
Summary: Enantioselective amino acid analysis is an important technique in pharmaceutical, biomedical, and food sciences. However, simultaneously analyzing all chiral proteinogenic amino acids using a single method with one column and one condition is still challenging. In this study, an enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS) assay using Chiralpak QN-AX as a chiral column was developed, enabling efficient separation of D- and L-amino acids.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Multidisciplinary
Muzaffar Iqbal, Essam Ezzeldin, Nazrul Haq, Prawez Alam
Summary: A novel UPLC-MS/MS assay utilizing dispersive liquid-liquid microextraction and ultrasound-assisted reverse extraction has been developed for determination of multiclass pharmaceuticals in water samples. The method showed specificity and reliability for trace analysis of target analytes in waste water samples.
Correction
Pharmacology & Pharmacy
Shishir Chourey, Qiuji Ye, Chintam Nagendra Reddy, Chantal Cossette, Sylvie Gravel, Matthias Zeller, Irina Slobodchikova, Dajana Vuckovic, Joshua Rokach, William S. Powell
BIOCHEMICAL PHARMACOLOGY
(2018)
Review
Chemistry, Multidisciplinary
Dajana Vuckovic
CHEMICAL COMMUNICATIONS
(2018)
Article
Pharmacology & Pharmacy
Chintam Nagendra Reddy, Hussam Alhamza, Shishir Chourey, Qiuji Ye, Vivek Gore, Chantal Cossette, Sylvie Gravel, Irina Slobodchikova, Dajana Vuckovic, Joshua Rokach, William S. Powell
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2018)
Article
Biochemical Research Methods
Irina Slobodchikova, Dajana Vuckovic
JOURNAL OF CHROMATOGRAPHY A
(2018)
Article
Chemistry, Medicinal
Shishir Chourey, Quji Ye, Chintam Nagendra Reddy, Rui Wang, Chantal Cossette, Sylvie Gravel, Irina Slobodchikova, Dajana Vuckovic, Joshua Rokach, William S. Powell
JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Biochemical Research Methods
Cian Monnin, Parsram Ramrup, Carolann Daigle-Young, Dajana Vuckovic
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2018)
Editorial Material
Biochemical Research Methods
Dajana Vuckovic
Article
Food Science & Technology
Irina Slobodchikova, Reajean Sivakumar, Md Samiur Rahman, Dajana Vuckovic
Article
Biochemical Research Methods
Rosalynde Ann Sonnenberg, Shama Naz, Lise Cougnaud, Dajana Vuckovic
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Pharmacology & Pharmacy
Qiuji Ye, Shishir Chourey, Chintam Nagendra Reddy, Rui Wang, Chantal Cossette, Sylvie Gravel, Irina Slobodchikova, Dajana Vuckovic, Joshua Rokach, William S. Powell
BRITISH JOURNAL OF PHARMACOLOGY
(2020)
Article
Biochemical Research Methods
Mariana S. T. Russo, Alexander Napylov, Alexandra Paquet, Dajana Vuckovic
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Alexander Napylov, Nathaly Reyes-Garces, German Gomez-Rios, Mariola Olkowicz, Sofia Lendor, Cian Monnin, Barbara Bojko, Clement Hamani, Janusz Pawliszyn, Dajana Vuckovic
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Endocrinology & Metabolism
Anne M. Evans, Claire O'Donovan, Mary Playdon, Chris Beecher, Richard D. Beger, John A. Bowden, David Broadhurst, Clary B. Clish, Surendra Dasari, Warwick B. Dunn, Julian L. Griffin, Thomas Hartung, Ping- Ching Hsu, Tao Huan, Judith Jans, Christina M. Jones, Maureen Kachman, Andre Kleensang, Matthew R. Lewis, Maria Eugenia Monge, Jonathan D. Mosley, Eric Taylor, Fariba Tayyari, Georgios Theodoridis, Federico Torta, Baljit K. Ubhi, Dajana Vuckovic
Article
Pharmacology & Pharmacy
Shishir Chourey, Qiuji Ye, Chintam Nagendra Reddy, Chantal Cossette, Sylvie Gravel, Matthias Zeller, Irina Slobodchikova, Dajana Vuckovic, Joshua Rokach, William S. Powell
BIOCHEMICAL PHARMACOLOGY
(2017)
Meeting Abstract
Chemistry, Multidisciplinary
Shishir Chourey, Qiuji Ye, Chintam Reddy, Rui Wang, Sylvie Gravel, Chantal Cossette, Irina Slobodchikova, Dajana Vuckovic, Matthias Zeller, William Powell, Joshua Rokach
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)