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Chemistry, Analytical
Sara Kheirandish, Shokoufeh Goudarzi, Maryam Amirahmadi, Shiva Ghareghani, Homanaz Ghafari, Bahram Daraei, Mahmoud Ghazi-Khansari
Summary: This study aimed to detect antibiotic residues in Iranian yogurt samples using a selective multi-residues method. The results showed no antibiotic contamination in the samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
D. Luque-Cordoba, F. Priego-Capote
Summary: Steroidogenesis is a crucial process in maintaining physiological levels of steroids in the body, and its deficiency can lead to various pathologies. A research has developed a high-throughput and fully automated method for quantifying multiple steroids in human serum, achieving high sensitivity and selectivity. This method was tested on human serum samples and showed satisfactory results in line with physiological ranges reported in literature.
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Biochemical Research Methods
Jia Zhan, Rong-rong Zhang, Xi-zhi Shi, Zhiqiang Huang, Guo-zhou Cao, Xian-feng Chen, Ling Hu
Summary: A rapid, simple, and versatile analytical method was developed for the simultaneous determination of 140 undesirable low-weight pesticides and mycotoxins in black tea. The method involved a combination of dual D-SPE with C-18 sorbent and anhydrous magnesium sulfate to eliminate interferences in the extract, maintaining the chromatographic column under ideal conditions for long-term satisfactory recoveries. Matrix-matched calibration curves were obtained for most target compounds with linear regression coefficients above 0.9900, and the limits of quantification (LOQs) ranged within 0.5-10.0 μg/kg.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Jakub Maculewicz, Piotr Stepnowski, Joanna Dolzonek, Anna Bialk-Bielinska
Summary: Imidazolium ionic liquids (ILs) are increasingly being used, and there is evidence of their presence in the environment. Due to their potential for bio-concentration, it is necessary to monitor the presence of these compounds in organisms from potentially contaminated waters. This study developed a novel analytical procedure for the determination of imidazolium ILs in mussel tissues using a combination of extraction techniques and LC-MS/MS analysis.
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Biochemistry & Molecular Biology
Daniela Maria Calucica, Costel-Valentin Manda, Amelia Maria Gaman, Stefan Raileanu, Liliana Stanca, Monica Denisa Elena Popescu, Olivia Garofita Mateescu, Andrei Bita, Octavian Croitoru, Simona-Daniela Neamtu
Summary: A simple and accurate LC-MS method was developed and validated for determining palbociclib and abemaciclib in human plasma. Solid phase extraction using Oasis PRiME HLB cartridges was used for sample preparation, providing clean extracts with a recovery extraction higher than 85%. The method can be used for quantifying the drugs in human plasma in routine analysis and proved to be useful in determining real plasma levels in cancer therapy patients.
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Food Science & Technology
Chujun Liu, Yawen Guo, Bo Wang, Lan Chen, Kaizhou Xie, Chenggen Yang
Summary: A simple and sensitive GC-MS/MS method was developed for screening penicillin G residues in poultry eggs, utilizing an external calibration method and electron impact ionization for analyte separation. The method validation demonstrated good linearity, accuracy, and precision, making it suitable for quantitative analysis.
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Food Science & Technology
Alka Kumari, Bhaswati Bhattacharya, Tripti Agarwal, Vijay Paul, Vaibhav Kumar Maurya, S. Chakkaravarthi, J. Simal-Gandara
Summary: To address the increasing demand for healthy and safe foods, researchers developed a sensitive and precise method for the detection and quantification of food contaminants like acrylamide, even in trace amounts.
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Chemistry, Analytical
Laure Wiest, Barbara Giroud, Azziz Assoumani, Francois Lestremau, Emmanuelle Vulliet
Summary: A multi-family analytical strategy for quantifying surfactants in surface waters was developed, optimized, and validated, with the method suitable for monitoring low concentrations of surfactants. Anionic, cationic, and non-ionic surfactants were detected in French river water samples, with median concentrations ranging from 24 ng/L to 4.6 μg/L.
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Food Science & Technology
Yujihn Kwon, Yongeui Koo, Yoonhwa Jeong
Summary: This study developed an analytical method to determine pyrrolizidine alkaloids in teas using liquid chromatography-tandem mass spectrometry combined with rapid-easy extraction. The method showed excellent repeatability, sensitivity, and reproducibility, and could be applied to various tea samples. Overall, the method allows complete separation of lycopsamine and intermedine peaks, making it useful for monitoring pyrrolizidine alkaloids in teas.
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Biochemical Research Methods
Thomas Meikopoulos, Olga Deda, Efstratios Karagiannidis, Georgios Sianos, Georgios Theodoridis, Helen Gika
Summary: In this study, a rapid quantitation method for acylcarnitines in human serum was developed and validated. The method was successfully applied in the analysis of samples from patients with coronary artery disease.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Masaru Taniguchi, Tomiaki Minatani, Hitoshi Miyazaki, Hitoshi Tsuchihashi, Kei Zaitsu
Summary: A highly sensitive quantification method using LC/MS/MS was developed for simultaneous analysis of 12 plant toxins in human serum. The method demonstrated excellent linearity, accuracy, precision, and sensitivity in validation studies. Application of this method to model samples confirmed its practicality for prompt decision-making in accidental poisonings involving toxic plants.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Raegyn B. Taylor, Yelena Sapozhnikova
Summary: This study evaluated and compared the performance of two instruments for the determination of per- and polyfluoroalkyl substances (PFAS) using a newly developed sample preparation method. The results showed that the method had better recoveries, matrix interferences, and co-extractive removal compared to the FDA and FSIS methods. Validation of the method in different matrices achieved acceptable recoveries and precision for the target analytes at various levels. The study also demonstrated the potential for future analysis of emerging alternatives using high-resolution mass spectrometry.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Jose Fernando Huertas-Perez, Pascal Mottier, Quentin Basle, Shi Ying Tan, Monika Kopec-Durska, Patrycja Zawada, Antoinette Burton, Ashley Griffin, Maria Guadalupe Sanchez-Calderon
Summary: This study evaluated an analytical procedure for routine monitoring of chlorate and perchlorate in a broad range of food matrices, using liquid chromatography tandem mass spectrometry. The method demonstrated high accuracy and is suitable for monitoring in various food matrices.
MICROCHEMICAL JOURNAL
(2022)
Article
Environmental Sciences
Ming Zheng, Suwen Tang, Yangyang Bao, Kevin D. Daniels, Zuo Tong How, Mohamed Gamal El-Din, Jie Wang, Liang Tang
Summary: A fully automated SPE coupled with UHPLC-MS/MS method was developed for analysis of antibiotics with optimized sample preparation and instrument parameters. This method can meet the needs of trace antibiotic residues detection in waters with high sensitivity and precision.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Medicinal
Guanxuanzi Zhang, Na Zhang, Liuhan Dong, Nan Bai, Yun Cai
Summary: A LC-MS/MS method for quantifying contezolid in plasma and cerebrospinal fluid (CSF) has been developed and verified in this study. Using linezolid as the internal standard, protein precipitation was performed. The LC-MS/MS analysis was performed using an Agilent EclipsePlus C18 column and acetonitrile and water mobile phases. The method showed good linearity, precision, and recovery rates in both plasma and CSF samples.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
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Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
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Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
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Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)