Review
Chemistry, Analytical
Yan Wu, Ning Zhang, Kaixing Luo, Yanjuan Liu, Zhengwu Bai, Sheng Tang
Summary: This article reviews the recent advances in efficient chromatographic separation materials developed in 2020-2021, focusing on their synthesis strategies, performance evaluation, and applications in hydrophilic interaction chromatography and chiral separation. The review provides readers with a comprehensive overview of the significance of these materials in current and future separation technologies.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Bei Liu, Hui Li, Kaijun Quan, Jia Chen, Hongdeng Qiu
Summary: This review article focuses on the application of periodic mesoporous organosilica (PMO) materials with organic functionalities in chromatographic separation, including packed columns and monolithic columns. The correlation between PMO chromatographic stationary phase compositions and chromatographic performance is discussed by summarizing the functional bridges, synthesis methods, and modification methods. The research directions for further development of a more highly efficient PMO chromatographic column are also outlined.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Tiantian Si, Xiaofeng Lu, Haixia Zhang, Shuai Wang, Xiaojing Liang, Yong Guo
Summary: This review provides a brief summary of the performances and recent applications of metal-organic frameworks (MOFs) based core-shell composites as stationary phases in high-performance liquid chromatography. It also discusses in detail the application of MOF-based composites for separating analytes under different conditions.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Isabela de Paula Lima, Sandy Polycarpo Valle, Marcone Augusto Leal de Oliveira, Flavia Ferreira de Carvalho Marques, Fernando Antonio Simas Vaz
Summary: Monolithic stationary phases have been widely studied in separation science, particularly in liquid chromatography and capillary electrochromatography, for over three decades. The production of monolithic columns involves various steps, including precursor solution preparation, tubing pretreatment, solution introduction, in situ reaction, and posttreatment. Different types of processes and reactions, such as sol-gel, polycondensation, free radical polymerization, and click reactions, are discussed. This work aims to provide an overview of the production process and the characteristics of monolith compositions, as well as to encourage researchers in finding suitable monoliths for analytical separations.
MICROCHEMICAL JOURNAL
(2023)
Review
Biochemical Research Methods
Mohamed Hefnawy, Manal El-Gendy, Huda Al-Salem, Hanin Marenga, Adel El-Azab, Alaa Abdel-Aziz, Ali El Gamal, Mohammed Alanazi, Ahmad Obaidullah, Abdullah Al-Hossaini, Abdullah Hefnawy
Summary: Monolithic columns are excellent substitutes for conventional particle-packed columns, offering higher permeability and lower flow resistance. They play an important role in the separation of complex biological samples and are widely used in fast and efficient multi-dimensional separation systems and hyphenated systems with mass spectrometers.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Materials Science, Multidisciplinary
Li Gao, Huiying Zuo, Aikun Han, Yaming Sun, Xue Ma, Lijun He, Wenjie Zhao
Summary: This study investigates two versions of reversed-phase/hydrophilic interaction mixed-mode core-shell stationary phases for high-performance liquid chromatography using porous organic polymers as the shell material. The researchers successfully obtained the SiO2@CHPCy-DB core-shell stationary phase by growing a porous shell on the surface of silica. The SiO2@CHPCy-DB exhibited reversed-phase/hydrophilic interaction mixed-mode with multiple retention mechanisms. Additionally, the role of the building blocks in porous organic polymers in regulating and improving separation was assessed by comparing the separation behavior of the homemade columns with a commercial column. The successful determination of trace analytes in complex samples using the SiO2@CHPCy-DB column demonstrates its potential.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Chemistry, Analytical
Marwa A. El-Wahab Mubarak, Ramzia El-Bagary, M. Abdul-Azim Mohammad, Nisreen F. Abo-Talib, Ehab F. Elkady
Summary: Two simple, sensitive, and rapid liquid chromatographic methods were developed for the determination of palonosetron hydrochloride and its related impurities. Both Method A and Method B showed excellent linearity and detection limits, making them suitable for quality control of palonosetron in pharmaceutical dosage forms.
MICROCHEMICAL JOURNAL
(2021)
Review
Chemistry, Analytical
Xiaoping Wang, Hui Li, Kaijun Quan, Liang Zhao, Hongdeng Qiu, Zuguang Li
Summary: Separation and identification of chiral enantiomers are increasingly important in various fields like pharmaceutical production, chemical intermediates preparation, and biochemistry. Cellulose and its derivatives, with strong chiral recognition ability, large loading capacity and ease of functionalization, have shown excellent performance in enantioseparation, indicating great potential in chiral separation. This review systematically summarizes the types of cellulose derivatives, preparation of cellulose-functionalized chiral stationary phases, and their recent applications in chiral chromatographic separation, aiming to provide a useful reference for researchers and inspire new discoveries in the field.
Review
Chemistry, Analytical
Rustem Kecili, Fatemeh Ghorbani-Bidkorbeh, Ibrahim Dolak, Gurbet Canpolat, Muharrem Karabork, Chaudhery Mustansar Hussain
Summary: This paper introduces the superior features of nanomaterials, especially magnetic nanoparticles, and highlights the latest progress and applications in their development for extraction and chromatographic applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Qiong Zheng, Jiajia Huang, Yanting He, Huan Huang, Yin Ji, Yongfan Zhang, Zian Lin
Summary: A single-crystalline covalent organic framework (COF-300) packed column was developed for the successful separation of positional isomers in high-performance liquid chromatography (HPLC), demonstrating the great potential of single-crystalline COFs in separation science.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Zhihua Song, Yanqin Song, Yinghao Wang, Jinqiu Liu, Yumeng Wang, Wen Lin, Yaqi Wang, Jinhua Li, Jiping Ma, Gangqiang Yang, Lingxin Chen
Summary: In this study, zidovudine imprinted polymers coated silica stationary phases (MIPs/SiO2 SPs) were successfully synthesized and demonstrated superior chromatographic performances in separation of nucleoside analogues and ginsenosides.
Article
Chemistry, Multidisciplinary
Geng Li, Xiao Dai, Yixuan Min, Lili Zhang, Jun Shen, Yoshio Okamoto
Summary: Four hybrid bead-type CSPs with organic contents higher than 20% were developed using a modified Stober process in basic conditions, showing better enantioseparation properties than previous analogues. These hybrid CSPs exhibited higher enantioselectivity than the commercial Chiralpak IB in some cases.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Biochemical Research Methods
Di Zhang, Yuchen Zhu, Yan Jin, Boxin Chen, Bin Zhao, Lei Li, Haimei Zhou, Xinxin Li, Dan Zheng, Fei Feng
Summary: A composite material, FG-HKUST-1, was synthesized by modifying the HKUST-1 material with multilayer FG. The composite material showed excellent separation performance on a micro gas chromatography column, especially for light hydrocarbons with significant concentration differences.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Ziqi Sun, Meiling Qi
Summary: This study reports the investigation of a new poly(propylene-carbonate) copolymer terminated by the adamantane cages (APPC) as the stationary phase for gas chromatographic (GC) analyses. The separation performance of the APPC column was compared to reference columns coated with poly(propylene carbonate) diol (PPCD), polyethylene glycol (PEG), and polysiloxane. The results showed that the APPC column has significantly advantageous performance and excellent repeatability and reproducibility.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Chemistry, Inorganic & Nuclear
Anna A. Kotova, Didier Thiebaut, Jerome Vial, Antoine Tissot, Christian Serre
Summary: Analytical chemistry is crucial for separating complex mixtures, and Metal-organic frameworks (MOFs) are promising porous hybrid solids with unique physico-chemical properties, which can be applied in chromatography and solid-phase extraction to address challenges in these fields.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Biochemical Research Methods
Shirui Yang, Wenxiang Zhang, Zheyi Liu, Ziyang Zhai, Xudong Hou, Ping Wang, Guangbo Ge, Fangjun Wang
Summary: This paper systematically investigates the molecular interactions between HSA and various therapeutic drugs using a mass spectrometry-based strategy, revealing the major ligand binding sites of HSA and newly characterized drug-binding areas. The MS-LRP strategy may have important application prospects in pharmacodynamics, pharmacokinetics, and safety evaluation of small-molecule drugs.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Pan Luo, Zheyi Liu, Tingting Zhang, Xiaolei Wang, Jing Liu, Yiqiang Liu, Xiaohu Zhou, Yang Chen, Wenrui Dong, Chunlei Xiao, Yan Jin, Xueming Yang, Fangjun Wang
Summary: This study presents a novel method for peroxide-free photochemical oxidation of proteins (PPOP) mediated by chloride ions, which can sensitively monitor protein high-order structures and be applied to study protein-drug interfaces.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Min He, Wenxiang Zhang, Zheyi Liu, Lingqiang Zhou, Xiaoming Cai, Ruibin Li, Yuanjiang Pan, Fangjun Wang
Summary: In this study, the molecular interaction details of human serum albumin (HSA) with Au and SiO2 nanoparticles were systematically investigated using lysine reactivity profiling mass spectrometry (LRP-MS). The results demonstrated that Au nanoparticles exhibited better biocompatibility than SiO2 nanoparticles, and the surface charge state of the nanoparticles played a more important role in the combination with HSA than particle size in the range of 15-40 nm.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Wenxiang Zhang, Zheyi Liu, Ye Zhou, Can Lai, Binwen Sun, Min He, Ziyang Zhai, Jian Wang, Qi Wang, Xian Wang, Fangjun Wang, Yuanjiang Pan
Summary: The molecular mechanisms of dynamic interactions between perfluorooctanoic acid (PFOA) and serum proteins remain unclear. Researchers developed a structural strategy to profile the molecular details of interactions among PFOA, serum albumin (SA), and 13-cyclodextrin (13-CD). They found that 13-CD can disrupt PFOA combinations with serum albumin, providing molecular insights into PFOA toxicology.
Article
Chemistry, Applied
Pan Gu, Shizhe Qi, Ziyang Zhai, Jing Liu, Zheyi Liu, Yan Jin, Yanxia Qi, Qiancheng Zhao, Fangjun Wang
Summary: Using a proteomic strategy, this study analyzed the effects of different thermal processing methods on the protein composition of sea cucumbers. Despite a relatively high protein loss rate, the main types of protein composition were retained. The findings have important implications for optimizing thermal processing in the sea cucumber industry.
Article
Environmental Sciences
Shiwen Liu, Heng Zhao, Zheyi Liu, Wenxiang Zhang, Can Lai, Shan Zhao, Xiaoming Cai, Yanxia Qi, Qiancheng Zhao, Ruibin Li, Fangjun Wang
Summary: In this study, we developed a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR MS) strategy for the accurate characterization of different types of micro/nanoplastics. By optimizing the sample preparation and detection conditions, we improved the detection signal-to-noise ratios and achieved unambiguous elucidation of polymer constitutions of environmental micro/nanoplastics.
Article
Nanoscience & Nanotechnology
Guofang Zhang, Yalin Cong, Feng-Liang Liu, Jiufeng Sun, Jiantian Zhang, Guoli Cao, Lingqiang Zhou, Wenjie Yang, Qingle Song, Fangjun Wang, Ke Liu, Jing Qu, Jing Wang, Min He, Shun Feng, Didar Baimanov, Wei Xu, Rong-Hua Luo, Xin-Yan Long, Shumin Liao, Yunping Fan, Yu-Feng Li, Bai Li, Ximing Shao, Guocheng Wang, Lijing Fang, Huaiyu Wang, Xue-Feng Yu, Yan-Zhong Chang, Yuliang Zhao, Liang Li, Peng Yu, Yong-Tang Zheng, Diana Boraschi, Hongchang Li, Chunying Chen, Liming Wang, Yang Li
Summary: A biocompatible and antiviral two-dimensional nanomaterial has been reported, which can firmly adsorb the virus and inhibit viral infection through conformational changes induced by interaction with the spike protein. This study proposes CIPS as a promising nanodrug for future anti-SARS-CoV-2 therapy, and also as a decontamination agent and surface-coating material to reduce SARS-CoV-2 infectivity.
NATURE NANOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Lingqiang Zhou, Zheyi Liu, Yongjie Guo, Shiwen Liu, Heng Zhao, Shan Zhao, Chunlei Xiao, Shun Feng, Xueming Yang, Fangjun Wang
Summary: In this study, the effects of charge state and crown ether (CE) complexation on the gas-phase native-like protein structure were systematically investigated by integrating native mass spectrometry (nMS) with 193 nm ultraviolet photodissociation (UVPD). The results showed that CE complexation can greatly alleviate the structure disruption caused by the intramolecular solvation of charged side chains, resulting in a more stable and native-like protein structure in the gas phase.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Biochemical Research Methods
Binwen Sun, Zheyi Liu, Jin Liu, Shan Zhao, Liming Wang, Fangjun Wang
Summary: In this review, the authors provide an overview of common and emerging proteases used in proteomics analysis in the past 5 years. They focus on the unique cleavage features of these proteases and their specific applications in protein characterization, PTM discovery, and sequencing. Additionally, the authors summarize the use of proteases in structural proteomics and protein function analysis. They also discuss future directions for the development of new proteases and their applications in proteomics.
Article
Nanoscience & Nanotechnology
Jing Liu, Ji Lv, Zheyi Liu, Zheng Fang, Can Lai, Shan Zhao, Mingliang Ye, Fangjun Wang
Summary: In this study, it was discovered that interfacial hydrophobic hydration significantly enhances H-bond networks at the interface between a glycan-modified adsorbent and a methanol-water-acetonitrile ternary solution. This enhancement promotes the adsorbent-solution heterogeneous glycan-glycan recognition and interaction, leading to improved glycopeptide enrichment. Compared with the commonly used hydrophilic-interaction enrichment strategy, more intact N- and O-glycopeptides are identified and the enrichment recoveries are significantly increased. This study also provides in-depth profiling of N- and O-glycosylation on SARS-CoV-2 S1 and hACE2 proteins.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Yu Bai, Zheyi Liu, Yuanqing Li, Heng Zhao, Can Lai, Shan Zhao, Kaixian Chen, Cheng Luo, Xueming Yang, Fangjun Wang
Summary: In this study, the dynamic molecular interactions and overall protein assembly of CDK12/CDK13-CycK complexes under the modulation of small molecule inhibitors were systematically investigated using the structural mass spectrometry (MS) methods of lysine reactivity profiling (LRP) and native MS (nMS). The complementary results of LRP and nMS provided essential structure insights, including inhibitor binding pocket, binding strength, interfacial molecular details, and dynamic conformation changes. The binding of the inhibitor SR-4835 destabilized the CDK12/CDK13-CycK interactions in an unusual allosteric activation way, offering a novel alternative for kinase activity inhibition. The study highlights the great potential of LRP combined with nMS for the evaluation and rational design of effective kinase inhibitors at the molecular level.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemical Research Methods
Zheyi Liu, Shirui Yang, Lingqiang Zhou, Min He, Yu Bai, Shan Zhao, Fangjun Wang
Summary: Understanding the interplay between proteins and materials is crucial for a wide range of applications including safety evaluation of biomedical micro/nanomaterials, toxicity estimation, design of nano-drugs, and catalytic activity improvement of bio-inorganic functional hybrids. However, characterizing the molecular details of protein-micro/nanomaterial hybrids at the interface remains a significant challenge. This protocol presents a lysine reactivity profiling-mass spectrometry strategy to determine the interacting regions of a protein with micro/nanomaterials. Lysine residues, which are commonly found on hydrophilic protein surfaces, exhibit reactivity that depends on the accessibility of their amine groups. By analyzing the reactivity of lysine residues, useful information can be obtained about the protein localization orientation, interaction sequence regions, binding sites, and modulated protein structures in the protein-material hybrids. The protocol also includes optimized two-step isotope dimethyl labeling strategy, protein digestion, elution from materials, data processing, and interfacial structure analysis. The entire protocol takes approximately 3-5 days to complete, with the two-step isotope labeling steps requiring about 5 hours.
Article
Biochemistry & Molecular Biology
Xiong Chen, Shanping Ji, Zheyi Liu, Xiao Yuan, Congsheng Xu, Ruxi Qi, An He, Heng Zhao, Haiping Song, Chunlei Xiao, Weina Gao, Peng R. Chen, Ray Luo, Pengfei Li, Fangjun Wang, Xueming Yang, Ruijun Tian
Summary: Identification of the tyrosine phosphorylation (pY)-dependent interactome of immune co-receptors is crucial for understanding signal pathways involved in immunotherapy. In this study, a photoaffinity-based chemical proteomic approach was used to dissect the motif-specific cytoplasmic interactomes of CD28, a critical immune co-receptor. The study identified specific interactions of phospholipase PLCG1 with the Y191 motif and the kinase PKCq with the previously undefined pY218 motif. This synthetic CD28(cyto)-based photoaffinity proteomic approach can be applied to study other immune co-receptors with multiple pY sites.
CELL CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Pan Luo, Zheyi Liu, Tingting Zhang, Xiaolei Wang, Jing Liu, Yiqiang Liu, Xiaohu Zhou, Yang Chen, Guangjin Hou, Wenrui Dong, Chunlei Xiao, Yan Jin, Xueming Yang, Fangjun Wang
Summary: This study describes the development of direct photochemical bromination and iodization of unprotected proteins and peptides, using 193 nm laser photoexcitation of halide ions to form active radical species. High protein modification efficiency (>90%) can be achieved under ultra-short irradiation of a single pulse, while retaining the compact native protein structure.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Zheyi Liu, Wenxiang Zhang, Binwen Sun, Yaolu Ma, Min He, Yuanjiang Pan, Fangjun Wang
Summary: Researchers developed a TILLRP strategy using mass spectrometry to probe the key lysine sites involved in the binding of SARS-CoV-2 S1 to ACE2, with significant alterations in lysine reactivities observed at the interface of S1-RBD during protein interactions.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
