Article
Biochemistry & Molecular Biology
Lanxiang Dai, Ting Wang, Yantao Liu, Yanjiao Lan, Li Ji, Jianxin Jiang, Pengfei Li
Summary: Circular Gleditsia sinensis gum, Gleditsia microphylla gum, and tara gum are galactomannans with similar mannose/galactose molar ratios. The hydrophobic interactions and critical aggregation concentrations of the galactomannans were compared using a fluorescence probe technique. The results showed that increasing galactomannan concentration led to the formation of hydrophobic domains, while temperature and urea concentration affected the destruction and formation of these domains, respectively.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Sinduja Malarkodi Elangovan, S. Muthu Kumar, V. Sindhu, Balaji Mahendiran, Shalini Muthusamy, Gopal Shankar Krishnakumar
Summary: This study focuses on the extraction, purification, and characterization of galactomannan from Borassus labellifer (Linn.) seeds for its potential use in tissue engineering. The isolated galactomannan, known as BFG, showed promising compositional and functional properties. The BFG demonstrated a non-Newtonian and pseudo-plastic behavior, possessed an amorphous structure with the presence of pores and cervices, and exhibited favorable biological activity against fibroblast cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Wei Xu, Minghui Han, Yantao Liu, Yana Zhu, Fenglun Zhang, Fuhou Lei, Kun Wang, Li Ji, Jianxin Jiang
Summary: This study identified structural and physicochemical changes in Sophora japonica f. pendula galactomannan (SJ-GM) during the late deposition stage. These changes included a decreased mannose to galactose ratio, leading to a more compact conformation, higher molecular weight, increased hydrophobicity, and greater solution viscosity. Importantly, SJ-GM exhibited stronger gel strength compared to other galactomannans, making it a promising alternative.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Applied
Xu Zhang, Lanxiang Dai, Pengfei Li, Ting Wang, Liting Qin, Jing Xiang, Huilin Chang
Summary: Fluorescence probing was used to study hydrophobic interactions of galactomannan (GM) obtained from different gums at various M/G ratios. The critical aggregation concentration and I-1/I-3 ratio were found to be highly dependent on temperature, additives, and galactose distribution, indicating the complex nature of hydrophobic interactions in GM.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Applied
Eline Van Wayenbergh, Jolien Verheijen, Niels A. Langenaeken, Imogen Foubert, Christophe M. Courtin
Summary: In this study, a new method for analyzing retinyl palmitate without the need for saponification was developed and validated. The results showed that the improved extraction protocols had low limits of detection and quantification, as well as good recovery and precision for retinyl palmitate extraction from model systems. For complex food matrices, the chloroform/methanol extraction protocol demonstrated the best performance characteristics.
Article
Biotechnology & Applied Microbiology
Wei Li, Meina Lin, Chenchao Wang, Yongping Lu, Yu Sui, Xiang Ni, Jing Guo, Miao Jiang, Liqun Yang, Hong Cui
Summary: This study successfully prepared blends of PTMC and PEGDA through photopolymerization. The results showed that the introduction of UV-crosslinked PEGDA significantly improved the resistance of PTMC to lipase erosion, and the degradation rate of PTMC could be controlled by adjusting the degree of crosslinking.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Cristina Coman, Niculina Hadade, Szilard Pesek, Radu Silaghi-Dumitrescu, Augustin C. Mot
Summary: In this study, the isolated Sclerotinia sclerotiorum laccase was used to degrade sodium diclofenac, a commonly found nonsteroidal anti-inflammatory drug in water. The laccase degrades diclofenac by generating radicals and facilitating their rapid precipitation, resulting in more than 96% degradation. The degradation products were analyzed using various techniques to determine their structures and compositions.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Environmental Sciences
Kristinka Vinkovic, Marina Vukoje, Mirela Roz, Nives Galic
Summary: This study monitored the anaerobic degradation process of thermal printing paper containing embedded BPA in soil. The results showed that the mass fraction of BPA in papers decreased over time under simulated conditions of deep layers in a landfill site.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Biochemical Research Methods
Burki Rajendar, M. V. N. Janardhan Reddy, Ch N. Suresh, Sampreeth Kumar Gambheerrao, Ramesh Matur
Summary: Viral vaccines are commonly produced using formaldehyde and Triton X-100, with a newly developed RP-HPLC-UV method for quantifying residual impurities in vaccines. The method offers rapid quantification and improved peak asymmetry for both Triton X-100 and formaldehyde, making it valuable for vaccine manufacturing and release.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Zane Temova Rakusa, Mitja Pislar, Albin Kristl, Robert Roskar
Summary: Vitamin D3 stability in aqueous media is influenced by various factors, with metal ions and acidic conditions having the main destabilizing effect. The addition of EDTA, ascorbic acid, and citric acid can successfully stabilize Vitamin D3, with EDTA showing the most significant stabilizing effect. There was no observed synergism among antioxidants.
Article
Pharmacology & Pharmacy
Emily Yii Ling Wong, Gabriel Onn Kit Loh, Kok Khiang Peh
Summary: A simple and sensitive stability-indicating HPLC-UV method was developed and validated for the determination of montelukast in chewable tablet formulation. Stress degradation studies revealed sensitivity of montelukast sodium to photo degradation, oxidation, and acid hydrolysis.
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Engineering, Environmental
Arbab Tufail, Sultan Alharbi, Jawad Alrifai, Ashley Ansari, William E. Price, Faisal Hai
Summary: The study found that enzymatic pre-treatment in the enzymatic membrane bioreactor ultraviolet photolysis system resulted in better degradation of trace organic contaminants and fewer by-products with lower abundance.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2021)
Article
Biochemical Research Methods
Marcilio Cunha-Filho, Jessika Layane Rocha, Natane C. B. Duarte, Livia L. Sa-Barreto
Summary: This study aimed to develop and validate an analytical method using HPLC for determining propranolol in different skin layers, which was proven to be selective, linear, sensitive, precise, and successfully applied in skin permeation assays.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Chemistry, Analytical
Momochika Kumagai, Sanae Kato, Nanami Arakawa, Mika Otsuka, Takahisa Hamano, Nobuyuki Kashiwagi, Akira Yabuki, Osamu Yamato
Summary: The quantification of histidine-containing dipeptides in dolphin serum was successfully achieved using an improved and validated HPLC method, demonstrating reliability and simplicity in assessing the health condition of dolphins. The method showed good accuracy and precision, making it a useful tool for evaluating health conditions in dolphins and potentially in other biological samples.
Article
Biochemistry & Molecular Biology
Rosemary A. Bayne, Uma Jayachandran, Aleksandra Kasprowicz, Stefan Bresson, David Tollervey, Edward W. J. Wallace, Atlanta G. Cook
Summary: Ssd1, a conserved fungal RNA-binding protein, plays important roles in stress responses, cell division, and virulence. Through in vivo cross-linking and cDNA analysis, precise binding sites of Ssd1 on RNA were identified, particularly enriched in the 5' untranslated regions of mRNAs encoding cell wall proteins. The structure-guided mutations of Ssd1 prevent its binding to RNA, providing insights into the evolution of regulatory elements in the RNase II family.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Applied
Celine Fantou, Sebastien Comesse, Frederic Renou, Michel Grisel
CARBOHYDRATE POLYMERS
(2019)
Article
Chemistry, Medicinal
Florine Eudier, Nicolas Hucher, Celine Picard, Geraldine Savary, Michel Grisel
CHEMICAL RESEARCH IN TOXICOLOGY
(2019)
Article
Biophysics
Florine Eudier, Deborah Hirel, Michel Grisel, Celine Picard, Geraldine Savary
COLLOIDS AND SURFACES B-BIOINTERFACES
(2019)
Article
Dermatology
Geraldine Savary, Laura Gilbert, Michel Grisel, Celine Picard
SKIN RESEARCH AND TECHNOLOGY
(2019)
Article
Chemistry, Applied
Celine Fantou, Sebastien Comesse, Frederic Renou, Michel Grisel
CARBOHYDRATE POLYMERS
(2019)
Article
Chemistry, Physical
Sabine Vassaux, Geraldine Savary, Loic Le Pluart, Michel Grisel
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2019)
Article
Chemistry, Multidisciplinary
Florine Eudier, Michel Grisel, Geraldine Savary, Celine Picard
Review
Chemistry, Physical
Patrycja Brudzynska, Alina Sionkowska, Michel Grisel
Summary: This review provides a report on the properties and recent research advances in the application of plant-derived colorants in food, cosmetics, and textile materials. It evaluates various colorants and discusses future aspects regarding their applications in the coloration of various materials.
Article
Chemistry, Physical
Patrycja Brudzynska, Alina Sionkowska, Michel Grisel
Summary: This research explores the dyeing of leather fabric using plant-derived colorants and natural additives. The results show that the applied colorants have the potential to dye leather fabrics without impacting the environment, and the natural additives can positively influence the dyeing process.
Article
Chemistry, Applied
Marie Jaricot, Catherine Malhiac, Christina Chao, Fabien Merlaud, Michel Grisel, Geraldine Savary
Summary: A specific methodology combining sensory and chemical analyses was used to characterize the residual odor of two fatty esters and determine its origin. The study found that the odor of isononyl isononanoate is derived from isononyl alcohol, while the odor of ethyl oleate mainly comes from esters and aldehydes.
INTERNATIONAL JOURNAL OF COSMETIC SCIENCE
(2022)
Article
Materials Science, Multidisciplinary
Benjamin Barthod-Malat, Maxime Hauguel, Karim Behlouli, Michel Grisel, Geraldine Savary
Summary: In the automotive sector, nonwoven preforms made from natural and thermoplastic fibers are widely used to manufacture vehicle interior parts. However, these natural fiber composites (NFCs) release volatile organic compounds (VOCs) and odors, which hinder their widespread use. This study examines the effect of compression molding temperature on the release of VOCs and odors in flax/PP nonwoven composites. Real-time monitoring of core temperature and chemical analysis using HS-SPME and GC-MS were conducted to understand the changes in VOCs composition and odor intensity. The results allow for optimization of molding temperature to minimize odorous NFC materials.
Article
Chemistry, Physical
Mira Abou Dib, Marie Lemarchand, Ecaterina Gore, Michel Grisel
Summary: The emulsification properties of hydrophobically modified xanthan (HMX) obtained through green esterification using alkenyl succinic anhydrides (ASA) as grafting agents were investigated. The study found that the new-brand bio-sourced HMX showed better emulsifying potential compared to pristine xanthan. The stability of the emulsions was studied at different levels and it was found that increasing grafting density or hydrophobic chain length improved the stability of the emulsions.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Chemistry, Applied
Mira Abou Dib, Ecaterina Gore, Michel Grisel
Summary: The aim of this study is to investigate the properties and stability of hydrophobically modified xanthan (HMX) obtained via green esterification. Results show that grafting alkenyl moieties stabilize the thermal properties of xanthan and decrease its intrinsic viscosity. The rheological properties of HMX are enhanced, with an increase in viscosity and relaxation time, and an improvement in viscoelastic properties. Additionally, HMX slightly destabilizes the ordered conformation of xanthan in aqueous media.
FOOD HYDROCOLLOIDS
(2023)
Article
Polymer Science
Michele N. Fanwa, Catherine Malhiac, Nicolas Hucher, Arnaud M. Y. Cheumani, Maurice K. Ndikontar, Michel Grisel
Summary: The cosmetics industry is seeking efficient and sustainable substances to stabilize thermodynamically unstable emulsions or colloidal dispersions with high surface energy. Conventional surfactants have limitations due to environmental concerns and petroleum origin. This study explores the potential of a natural gum from Triumfetta cordifolia as an eco-friendly emulsifier/stabilizer for oil-in-water emulsions, showing promising results and applicability in cosmetics.
Article
Biochemistry & Molecular Biology
Patrycja Brudzynska, Marzanna Kurzawa, Alina Sionkowska, Michel Grisel
Summary: The application of plant-derived colorants in cosmetics or food not only provides desired color but also has antioxidant properties that benefit the skin. This study aimed to evaluate the antioxidant activity of purple and red plant-derived colorants using two methods: CUPRAC and DPPH free-radical scavenging activity. The results showed that UVC treatment had minimal influence on the antioxidant activity of the colorants.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
