Review
Chemistry, Analytical
Filip Dusa, Anna Kubesova, Jiri Salplachta, Dana Moravcova
Summary: Capillary isoelectric focusing (cIEF) is a powerful separation technique that requires the repeatable formation of a pH gradient and the use of internal standards to track its profile. This review provides an overview of available isoelectric point markers and their syntheses, as well as recent applications of cIEF in analyzing complex larger structures.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Jiaqi Wu, Will McElroy, Janusz Pawliszyn, Christopher D. Heger
Summary: This article reviews the applications of icIEF technology in the pharmaceutical industry, highlighting the benefits of whole-column imaging detection for capillary isoelectric focusing. It also explores recent research using this technology to support advancements in the therapeutic landscape.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Sidharth Modha, Carlos Castro, Hideaki Tsutsui
Summary: Over the last 10 years, paper has emerged as a promising substrate for affordable biosensors, with the field of paper-microfluidics rapidly evolving to handle complex devices. However, automated flow control on paper remains a challenge due to the lack of comprehensive theoretical models.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biochemical Research Methods
Lily Farmerie, Richard R. Rustandi, John W. Loughney, Mohamed Dawod
Summary: This review article highlights the significant recent advances in Isoelectric Focusing from 2015-2020, discussing principles, challenges, applications, and recent developments in various modes of Isoelectric Focusing on different platforms, as well as future directions for the technique.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Filip Dusa, Dana Moravcova, Karel Slais
Summary: We present a set of novel low-molecular-mass compounds with ampholytic properties that can be used as markers of isoelectric point in different isoelectric focusing formats. These compounds have excellent focusing properties and stability, making them suitable for various isoelectric focusing methods.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Ali Amini, Rosanne M. Guijt, Thomas Themelis, Jelle De Vos, Sebastiaan Eeltink
Summary: DLP 3D printing is an efficient additive manufacturing method for fabricating analytical microdevices. It utilizes a digital micromirror device to direct light and polymerize a liquid resin layer by layer. Advances in vat and lift design, projector technology, and resin composition enable the accurate fabrication of microchannel structures as small as 18 x 20 μm. This review presents the latest advances in DLP 3D printing technology, including instrument set-up and resin formulation, and highlights key efforts in fabricating microdevices for emerging (bio-)analytical chemistry applications such as colorimetric assays, extraction, and separation.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Mechanics
Gemma Houston, Paolo Capobianchi, Monica S. N. Oliveira
Summary: In this study, the flow of miscible viscous fluid systems through microfluidic flow focusing devices was investigated using experiments and numerical simulations. The results showed that the characteristics of the central focused outlet stream were influenced by the fluid viscosity ratio and the inlet velocity ratio. It was also observed that the width of the central focused outlet stream decreased with both the velocity ratio and the viscosity ratio. Additionally, the study provided further insight into the curved interface in parallel flow systems with high viscosity contrasts, highlighting the influence of inlet viscosity ratio and microchannel aspect ratio on the interface curvature.
Review
Chemistry, Analytical
Kuangjun Li, Jing Wu
Summary: Single particle analysis (SPA) is a popular technique to understand the atomic structure and interaction of particles. Microfluidics is widely used in SPA for sample preparation and particle analysis. This review provides an overview of four different microfluidic analysis platforms for particle separation and signal detection, highlighting their roles, benefits, technical issues, and scientific challenges. SPA using microfluidics is an important area of research with potential in improving our understanding of particle behavior.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Medicinal
Wisarut Khamcharoen, Kantima Kaewjua, Phanumas Yomthiangthae, Ananyaporn Anekrattanasap, Orawon Chailapakul, Weena Siangproh
Summary: In the last decade, the demand for drugs for biomedical applications has increased significantly due to the global increase in diseases. This has led to a need for analytical tools to monitor, treat, investigate, and control drug compounds in various matrices. Microfluidic paper-based analytical devices (μPADs) have gained attention in many analytical applications due to the benefits of their cellulose structure. They offer advantages such as self-pumping capability, low material costs, easy fabrication, and no requirement for external power sources. This review provides an overview of the designs and applications of μPADs in pharmaceutical analysis over the past decade. It discusses device designs, choice of paper material, and fabrication techniques, along with strategies for improving analytical performance and the detection of drug compounds. Recent advances and the challenges and potential of transitioning μPADs from the laboratory to commercialization are also discussed.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2022)
Review
Engineering, Biomedical
Cheng Lin, Liwu Liu, Yanju Liu, Jinsong Leng
Summary: Transcatheter closure is widely accepted as a highly effective treatment for abnormal blood flow in the heart, allowing closure of various congenital heart defects and stroke-related conditions. Advances in occlusion devices, including biodegradable and 3D-printed options, offer improved biocompatibility and customization. The review highlights materials, configurations, manufacturing methods, deployment strategies, and potential future directions for next-generation occlusion devices.
ACTA BIOMATERIALIA
(2021)
Review
Biochemical Research Methods
Gyula Vigh, Bohuslav Gas
Summary: In 1961, Svensson first described the principle of isoelectric focusing (IEF), which used a natural pH gradient formed by passing current through a sucrose density gradient-stabilized ampholyte mixture in a constant cross-section apparatus to separate ampholytic compounds. When polyacrylamide gel replaced the sucrose density gradient, the spatial and temporal stability of Svensson's pH gradient was lost, leading to a search for an explanation and improvement. It was later suggested that in the modern open-system IEF experiment, a two-stage process occurs, with a rapid first phase of linear pH gradient development followed by a slow second stage where isotachophoretic processes cause the pH gradient to decay as extreme pI CAs move into the electrode vessels.
Article
Biophysics
Yuxin Bayinqiaoge, Yuxin Zhang, Tim Cole, Jiahao Zheng, Jinhong Guo, Shi-Yang Tang
Summary: The effective analysis of bioparticles is important for early disease diagnosis. Optical microfluidic platforms have limitations in practical point-of-care testing due to their expensive components and bulky devices. Therefore, cost-effective non-optical microfluidic platforms are urgently needed. This review summarizes passive and active methods for bioparticle manipulation and surveys the latest progress in non-optical microfluidic strategies based on electrical, magnetic, and acoustic techniques for bioparticle detection. The challenges faced by current non-optical platforms in practical point-of-care testing and clinical applications are also discussed.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Multidisciplinary
Pengchao Zhang, Ning Shao, Lidong Qin
Summary: Cell-based living materials, including single cells, cell-laden fibers, cell sheets, organoids, and organs, have attracted intensive interests for their broad applications such as cancer therapy, regenerative medicine, and drug development. Recent advances in microfluidic platforms have enabled high-throughput, scalable, and efficient programming of these materials. This review highlights the progress of novel microfluidic platforms in programming cell-based living materials, providing insights into the future development in collaboration between materials scientists, engineers, biologists, and physicians for healthcare applications.
ADVANCED MATERIALS
(2021)
Review
Chemistry, Analytical
Waleed Alahmad, Ali Sahragard, Pakorn Varanusupakul
Summary: This review focuses on the recent developments of microfluidic paper-based analytical devices (mu PADs) for screening and detecting chromium species in environmental samples. The paper summarizes the chemistry and toxicity of chromium, selection of paper substrates, device fabrication methods, categorization of recent reports based on chromium species, and evaluation of mu PADs' performance. Future development and trends in the field of mu PADs are also discussed.
MICROCHEMICAL JOURNAL
(2021)
Article
Pharmacology & Pharmacy
Eunmi Ban, Aeri Kim
Summary: Coacervation is a liquid-liquid phase separation that occurs in solutions of macromolecules through self-assembly or electrostatic interactions. These coacervates, composed of biocompatible macromolecules, are actively being studied as platforms for encapsulating and delivering biomolecules. Despite their advantages, challenges remain in scaling up manufacturing methods for preclinical and clinical evaluations.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Haili Shi, Wouter N. Leonhard, Niels J. Sijbrandi, Mies J. van Steenbergen, Marcel H. A. M. Fens, Joep B. van de Dikkenberg, Javier Sastre Torano, Dorien J. M. Peters, Wim E. Hennink, Robbert Jan Kok
JOURNAL OF CONTROLLED RELEASE
(2019)
Article
Chemistry, Multidisciplinary
Ivan A. Gagarinov, Tiehai Li, Na Wei, Javier Sastre Torano, Robert P. de Vries, Margreet A. Wolfert, Geert-Jan Boons
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Review
Biochemical Research Methods
Javier Sastre Torano, Rawi Ramautar, Gerhardus de Jong
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2019)
Article
Medicine, Research & Experimental
Timo W. M. De Groof, Vida Mashayekhi, Tian Shu Fan, Nick D. Bergkamp, Javier Sastre Torano, Jeffrey R. van Senten, Raimond Heukers, Martine J. Smit, Sabrina Oliveira
MOLECULAR PHARMACEUTICS
(2019)
Article
Multidisciplinary Sciences
Jitske Jansen, Katja Jansen, Ellen Neven, Ruben Poesen, Amr Othman, Alain van Mil, Joost Sluijter, Javier Sastre Torano, Esther A. Zaal, Celia R. Berkers, Diederik Esser, Harry J. Wichers, Karin van Ede, Majorie van Duursen, Stephane Burtey, Marianne C. Verhaar, Bjorn Meijers, Rosalinde Masereeuw
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Medicine, Research & Experimental
Thomas K. van der Made, Michele Fedecostante, Daniel Scotcher, Amin Rostami-Hodjegan, Javier Sastre Torano, Igor Middel, Andries S. Koster, Karin G. Gerritsen, Vera Jankowski, Joachim Jankowski, Joost G. J. Hoenderop, Rosalinde Masereeuw, Aleksandra Galetin
MOLECULAR PHARMACEUTICS
(2019)
Article
Chemistry, Multidisciplinary
Javier Sastre Torano, Ivan A. Gagarinov, Gael M. Vos, Frederik Broszeit, Apoorva D. Srivastava, Martin Palmer, James Langridge, Oier Aizpurua-Olaizola, Victor J. Somovilla, Geert-Jan Boons
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Medicine, Research & Experimental
Yanna Liu, Luca Scrivano, Julia Denise Peterson, Marcel H. A. M. Fens, Irati Beltran Hernandez, Barbara Mesquita, Javier Sastre Torano, Wim E. Hennink, Cornelus F. van Nostrum, Sabrina Oliveira
MOLECULAR PHARMACEUTICS
(2020)
Article
Chemistry, Multidisciplinary
Javier Sastre Torano, Oier Aizpurua-Olaizola, Na Wei, Tiehai Li, Luca Unione, Gonzalo Jimenez-Oses, Francisco Corzana, Victor J. Somovilla, Juan M. Falcon-Perez, Geert-Jan Boons
Summary: This study introduces a method to identify isomeric N-glycans based on gas phase conformer distributions, using well-defined synthetic glycans for systematic research and calculating CD fingerprints through molecular dynamics simulations to establish a library for structure identification.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Plant Sciences
Erbil Gungor, Paul Brouwer, Laura W. Dijkhuizen, Dally Chaerul Shaffar, Klaas G. J. Nierop, Ric C. H. de Vos, Javier Sastre Torano, Ingrid M. van Der Meer, Henriette Schluepmann
Summary: Questions regarding in vivo substrates for proanthocyanidin biosynthesis and condensation remain unanswered, with gaps in understanding transport and biogenesis in 'tannosomes'. This study examined the evolution of proanthocyanidin biosynthesis in ferns, revealing (epi)catechin polymers synthesized in the sporophyte of Azolla ferns. The research also identified LAR and WLAR enzymes with differing substrate binding sites as an evolutionary innovation of the common ancestor of fern and seed plants.
Article
Biochemical Research Methods
Johannes J. M. Rood, Javier Sastre Torano, Victor J. Somovilla, Jos H. Beijnen, Rolf W. Sparidans
Summary: The quantitation method for erlotinib and other kinase inhibitors was successfully transferred from a Thermo TSQ Quantum Ultra system to a more sensitive Sciex QTRAP5500 system, with improved sensitivity and accuracy. The new method outperformed most other published methods in terms of simplicity and speed, making it suitable for therapeutic drug monitoring.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Nutrition & Dietetics
Gerlof P. Bosman, Sergio Oliveira, Peter J. Simons, Javier Sastre Torano, Govert W. Somsen, Leon M. J. Knippels, Rob Haselberg, Roland J. Pieters, Johan Garssen, Karen Knipping
Summary: This study identified lactosylation sites of BLG using CE-MS and found that lactosylated BLG significantly reduced antibody binding and degranulation of mast cells. These findings suggest that lactosylation may be a promising approach to decrease the allergenicity of cow's milk allergens.
Article
Biochemical Research Methods
Kevin Hooijschuur, Xiufen Liu, Anna Grootendorst, Ines Pieterman, Javier Sastre Torano
Summary: The use of mixed ion exchange particles and diatomite allows for high-capacity analysis of low-abundant compounds in CZE-DTIMS-MS, increasing sample injection volumes and flow rates.
Article
Multidisciplinary Sciences
Gael M. Vos, Kevin C. Hooijschuur, Zeshi Li, John Fjeldsted, Christian Klein, Robert P. de Vries, Javier Sastre Torano, Geert-Jan Boons
Summary: In this study, a method was developed to determine the structures of sialic acid variants and investigate their roles in biological and disease processes. The method utilized a library of synthetic sialic acids to establish collision cross section values of diagnostic fragment ions and analyzed sialic acids from biological samples to uncover their linkage types and acetylation patterns.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Pouya Zaree, Javier Sastre Torano, Cornelis A. M. de Haan, Richard A. Scheltema, Arjan Barendregt, Vito Thijssen, Guangyun Yu, Frits Flesch, Roland J. Pieters
Summary: This study investigated the binding characteristics of mono- and divalent ligands to LecA using various techniques, finding that the affinity of a divalent ligand was in the low-nanomolar range with a residence time of approximately 7 hours. The study highlights the unique and complementary insights provided by each technique into the chelation-based binding mode of the divalent ligand to the LecA tetramer.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
