Article
Biotechnology & Applied Microbiology
Qing Zhang, Yaozhong Chen, Lin Gao, Jian'gang Chen, Xin Ma, Dongbo Cai, Dong Wang, Shouwen Chen
Summary: This study optimized the expression cassette of VHB for the production of gamma-PGA, resulting in increased yields and improved oxygen transfer and ATP generation. The study also identified a suitable promoter for VHB expression and gamma-PGA synthesis, leading to further increases in gamma-PGA yield. Overall, this study achieved an efficient VHB expression cassette for oxygen transfer and gamma-PGA synthesis, with potential applications in the production of other metabolites.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Engineering, Chemical
Xiaoyu Wei, Lijie Yang, Haiyan Wang, Zhen Chen, Yiyuan Xu, Yue Weng, Mingfeng Cao, Qingbiao Li, Ning He
Summary: In this study, atmospheric and room temperature plasma was used for mutation breeding of Bacillus licheniformis CGMCC 2876, resulting in a mutant strain M32 with an increased production of poly-gamma-glutamic acid. Genomic resequencing and molecular docking analysis revealed the mutations in ppsC gene encoding lipopeptide synthetase and its enhanced binding with glutamate, which likely contribute to the increased poly-gamma-glutamic acid production. Metabolic mechanism analysis showed that the upregulated amino acids in M32 provided substrates for glutamate and enhanced the conversion between L- and D-glutamate. Moreover, the glycolytic pathway was enhanced, leading to a better capacity for glucose utilization. The maximum yield of poly-gamma-glutamic acid reached 14.08 g.L-1 with 30 g.L-1 glutamate.
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Bichan Li, Dongbo Cai, Shouwen Chen
Summary: Metabolic engineering of central carbon metabolism was applied to enhance gamma-PGA production in B. licheniformis WX-02. Overexpression of PdhABCD and CitA increased gamma-PGA yield by 34.93% and 11.14% respectively, while down-regulation of AceA and deletion of pflB further improved gamma-PGA production. Combinatorial metabolic engineering led to a 69.30% enhancement in gamma-PGA titer compared to the wild-type strain.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Fan Yang, Na Liu, Yaozhong Chen, Si Wang, Jun Liu, Ling Zhao, Xin Ma, Dongbo Cai, Shouwen Chen
Summary: This study presents a novel approach for improving gamma-PGA production by switching the cofactor specificity of glutamate dehydrogenase. The researchers used structural modeling and molecular docking to predict the binding modes of NADH and NADPH, and obtained site-specific mutants to alter cofactor specificity. The mutant RocGD276E showed the highest increase in gamma-PGA yield and decreased the yields of main by-products. Enzymatic properties confirmed that this mutant had higher affinity for NADH, shifting the coenzyme preference from NADPH to NADH while maintaining catalytic efficiency comparable with the wild-type.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lifei Guo, Liang Lu, Huichao Wang, Xiaoxing Zhang, Genan Wang, Tingbin Zhao, Guobao Zheng, Changsheng Qiao
Summary: This study found that the addition of FeSO4•7H(2)O in sugarcane molasses can increase the yield of γ-PGA and cell growth. Further investigation revealed that this additive can enhance the abundance of intracellular metabolites and upregulate the glycolysis pathway and TCA cycle.
MICROBIAL CELL FACTORIES
(2023)
Review
Biotechnology & Applied Microbiology
Zheng Zhang, Penghui He, Dongbo Cai, Shouwen Chen
Summary: This review focuses on the genetic and metabolic engineering of microorganisms for gamma-PGA production, including strategies to enhance raw materials utilization, precursor supply, gamma-PGA synthetase gene cluster, transcription regulation, cofactor regeneration, energy engineering, and blocking synthetic pathways of by-products. The expression of gamma-PGA synthetase, glutamate racemase, and gamma-PGA hydrolase were manipulated to obtain gamma-PGA with different configurations and molecular weights. The review also discusses the metabolic engineering of other hosts for high-level production of gamma-PGA.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xin Li, Haiqing Yang, Menglin Zhou, Yangyang Zhan, Jun Liu, Dazhong Yan, Dongbo Cai, Shouwen Chen
Summary: The study focused on cost-effective production of gamma-PGA from crude glycerol, achieving higher yields by optimizing nitrate feeding strategies, with constant-rate nitrate feeding and pH-feedback controlling playing key roles in improving production efficiency.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Jin Quan, Weiwei Zheng, Jingru Tan, Zewei Li, Meifang Wu, Seung-Beom Hong, Yanting Zhao, Zhujun Zhu, Yunxiang Zang
Summary: This study investigated the potential of glutamic acid (Glu) and poly-gamma-glutamic acid (gamma-PGA) biostimulants in enhancing the thermotolerance of Chinese cabbage under heat stress. The results showed that both Glu and gamma-PGA treatments improved the parameters of vegetative growth, gas exchange, and chlorophyll fluorescence. Moreover, these treatments increased the contents of total chlorophyll and carotenoid and reduced oxidative damage by enhancing the activity levels of antioxidant enzymes. Transcriptome analysis revealed that the expression levels of genes involved in various metabolic pathways were up-regulated by both Glu and gamma-PGA priming. Overall, Glu was found to be more effective than gamma-PGA in enhancing the thermotolerance of Chinese cabbage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Reina Deol, Ashweetha Louis, Harper Lee Glazer, Warren Hosseinion, Anna Bagley, Pete Chandrangsu
Summary: Zinc and copper are essential micronutrients that are toxic to bacteria at elevated concentrations. Bacteria have evolved defense mechanisms, including the production of extracellular polymeric substance poly-gamma-glutamate (gamma-PGA) to combat zinc and copper excess. This study provides new insight into metal resistance mechanisms and regulation of bacterial gamma-PGA biosynthesis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Agriculture, Multidisciplinary
Shengbao Chen, Jiaming Fu, Bo Yu, Limin Wang
Summary: In this study, a genetic operating system was developed to enable the production of ?-PGA with diverse molecular masses. The system was demonstrated to successfully produce ?-PGA with specific M (w) values, providing a research basis for sustainable production of desirable ?-PGA.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Food Science & Technology
Danfeng Li, Lizhen Hou, Yaxin Gao, Zhiliang Tian, Bei Fan, Fengzhong Wang, Shuying Li
Summary: This article provides a comprehensive overview of the production, biosynthetic pathway, and metabolic regulation of gamma-PGA. By studying the factors that influence its synthesis, it is possible to improve the yield and productivity of γ-PGA and promote its industrial application.
Article
Biochemical Research Methods
Liu-xiu Hu, Ming Zhao, Wen-song Hu, Meng-jie Zhou, Jun-bao Huang, Xi-lin Huang, Xu-li Gao, Ya-ni Luo, Chuang Li, Kun Liu, Zheng-lian Xue, Yan Liu
Summary: As a natural biological macromolecule, gamma-polyglutamic acid (gamma-PGA) plays a significant role in medicine, food, and cosmetic industries due to its unique properties. Current methods for increasing gamma-PGA yield are not effective due to the viscosity issue. This study successfully improved the yield of gamma-PGA by using a dynamic regulation strategy based on quorum sensing.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Hua Yuan, Liming Zhou, Zhiwen Qi, Changwei Zhang, Chengzhang Wang
Summary: This study focuses on a new preparation method for solanesol derivatives, aiming to use them effectively as drug carriers. The newly designed solanesol-based poly (glutamic acid) (Sol-PEG-PGA) showed good biocompatibility and high-efficiency anticancer activity.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Microbiology
Jian Xue, Tong Tong, Rui Wang, Yibin Qiu, Yian Gu, Liang Sun, Hong Xu, Peng Lei
Summary: The study reveals that gamma-PGA promotes biofilm formation and colonization of Bacillus atrophaeus NX-12, enhancing its biocontrol capability.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Dexin Wang, Xiaoping Fu, Dasen Zhou, Jiaqi Gao, Wenqin Bai
Summary: In this study, a novel gamma-PGA-producing strain Bacillus tequilensis BL01 was isolated, and genetic engineering strategies were implemented to improve gamma-PGA production. Compared with the wild type strain, the engineered strain showed a 2.17-fold and 1.32-fold increase in gamma-PGA production in flask and fermenter, respectively. Different molecular weights of gamma-PGA were successfully obtained, expanding its industrial applications.
MICROBIAL CELL FACTORIES
(2022)
Article
Agriculture, Multidisciplinary
Dian Zou, Yu Min, Yingli Liu, Xuetuan Wei, Jing Wang
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Lu Li, Dian Zou, Anying Ji, Yuxuan He, Yingli Liu, Yu Deng, Shouwen Chen, Xuetuan Wei
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2020)
Article
Biochemical Research Methods
J Un Xiao, Bing Peng, Zhaowei Su, Ankun Liu, Yajing Hu, Christopher T. Nomura, Shouwen Chen, Qin Wang
ACS SYNTHETIC BIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Dongbo Cai, Bowen Zhang, Jiang Zhu, Haixia Xu, Pei Liu, Zhi Wang, Junhui Li, Zhifan Yang, Xin Ma, Shouwen Chen
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Yangyang Zhan, Menglin Zhou, Huan Wang, Lixia Chen, Zhi Li, Dongbo Cai, Zhiyou Wen, Xin Ma, Shouwen Chen
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Qing Yang, Yongfu Yang, Ying Tang, Xia Wang, Yunhao Chen, Wei Shen, Yangyang Zhan, Junjie Gao, Bo Wu, Mingxiong He, Shouwen Chen, Shihui Yang
BIOTECHNOLOGY FOR BIOFUELS
(2020)
Article
Biotechnology & Applied Microbiology
Cong Jiang, Liying Ruan, Xuetuan Wei, Ailing Guo
BIOTECHNOLOGY LETTERS
(2020)
Article
Agriculture, Multidisciplinary
Dian Zou, Lu Li, Yu Min, Anying Ji, Yingli Liu, Xuetuan Wei, Jing Wang, Zhiyou Wen
Summary: By mining key genes and conducting genetic engineering, a Bacillus amyloliquefaciens strain was successfully constructed to enhance spermidine production, achieving the highest titer of spermidine reported to date.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Agriculture, Multidisciplinary
Kalekristos WoldemariamYohannes, Zhen Wan, Qinglin Yu, Hongyan Li, Xuetuan Wei, Yingli Liu, Jing Wang, Baoguo Sun
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Agriculture, Multidisciplinary
Yali Duan, Yun Du, Zhiqiang Yi, Zhe Wang, Xiaolin Pei, Xuetuan Wei, Mu Li
Summary: Fungal azaphilones have great potential in the food and pharmaceutical industries, but their production is currently low and genetic engineering is challenging. This study demonstrates a systematic metabolic engineering strategy to improve azaphilone production, providing a new approach for producing high-value chemicals.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Dian Zou, Changwen Ye, Yu Min, Lu Li, Liying Ruan, Zhifan Yang, Xuetuan Wei
Summary: This study modified Bacillus amyloliquefaciens to produce fermented soybean rich in lycopene, which exhibited strong antioxidant and anticoagulant activities, offering a new approach for enhancing the functionality of soybean.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Food Science & Technology
Dian Zou, Ziyue Zhao, Lu Li, Yu Min, Daiyuan Zhang, Anying Ji, Cong Jiang, Xuetuan Wei, Xian Wu
Summary: This review provides new insights on spermidine from its safety to its food processing, which will advance the commercial production and applications of spermidine-rich foods and nutraceuticals.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Food Science & Technology
Weijie Chen, Lu Li, Changwen Ye, Ziyue Zhao, Kuo Huang, Dian Zou, Xuetuan Wei
Summary: In this study, a high extracellular protein expression capacity host of Bacillus amyloliquefaciens, named BAX-10, was developed by deleting seven important extracellular protease genes, one important intracellular protease gene, and two redundant protein genes. The alkaline protease gene aprE from Bacillus subtilis D7 was expressed in BAX-10, resulting in higher alkaline protease activity compared to the control strain. Furthermore, the deletion of the ten genes did not negatively affect the growth of B. amyloliquefaciens. Therefore, B. amyloliquefaciens BAX-10 could be a potential platform host for expressing target extracellular proteins.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Yali Duan, Yingao Tan, Xuetuan Wei, Xiaolin Pei, Mu Li
Summary: In this study, a strategy based on mutant library was developed to generate orthogonal gene expression toolboxes. Nine DNA-binding residues of the transcription factor MaR were identified using molecular simulation and site-directed mutagenesis. The toolbox demonstrated fine gene expression tunability and high signal sensitivity through the introduction of chemically inducible expression modules, synthetic promoter elements, and protein-protein interactions between MaRs.
ACS SYNTHETIC BIOLOGY
(2023)