Article
Orthopedics
Ke Jiang, Yuling Li, Chao Xiang, Yan Xiong, Jiameng Jia
Summary: The study investigated the anti-adhesion and anti-inflammatory effects of TGF-beta 3 on flexor digitorum longus tendon repair in rats. Results demonstrated that TGF-beta 3 effectively protected against flexor tendon injury by regulating adhesion formation.
BMC MUSCULOSKELETAL DISORDERS
(2021)
Article
Critical Care Medicine
Oliver Pearce, Matthew T. Brown, Katrina Fraser, Luca Lancerotto
Summary: Flexor tendon injuries are common and often require thorough clinical assessment for repair. The key principles of repair include strong suture construct, minimizing gap formation, preserving blood supply, and providing a smooth repair interface. Adequate exposure of the zone of injury and preservation of neurovascular and pulley structures are essential.
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2021)
Review
Cell Biology
Craig E. Higgins, Jiaqi Tang, Stephen P. Higgins, Cody C. Gifford, Badar M. Mian, David M. Jones, Wenzheng Zhang, Angelica Costello, David J. Conti, Rohan Samarakoon, Paul J. Higgins
Summary: Tubulointerstitial fibrosis is a common feature in chronic renal disorders, driven by persistent TGF-beta 1 signaling. The transcriptional co-regulator p53 plays a key role in the fibrotic response induced by TGF-beta 1.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jiangcheng Shu, Lizhi Hu, Yichen Wu, Long Chen, Kai Huang, Zhaohui Wang, Minglu Liang
Summary: This study reveals that daidzein has an anti-fibrotic effect in myocardial fibrosis by reducing the activation of cardiac fibroblasts induced by TGF-beta 1, partially through regulating the TGF-beta 1/SMAD2/3 signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lisa Oezel, Marie Wohltmann, Nele Gondorf, Julia Wille, Irmak Gueven, Joachim Windolf, Simon Thelen, Carina Jaekel, Vera Grotheer
Summary: This study examined the role of TGF-beta 1 in Dupuytren's disease (DD), and found that impaired release and degradation of TGF-beta 1 are important factors in the pathological phenotype of this disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Xionghua Sun, Mei Zhu, Xihua Chen, Xiaogang Jiang
Summary: Research indicates that MYH9 plays a crucial role in TGF-beta 1-stimulated lung fibroblasts, and inhibition of MYH9 can suppress the differentiation of lung fibroblasts to myofibroblasts induced by TGF-beta 1.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, General & Internal
Ki-Eun Hwang, Hyo-Jin Kim, In-Sol Song, Chul Park, Jae Wan Jung, Sim Park, Seon-Hee Oh, Young-Suk Kim, Hak-Ryul Kim
Summary: Salinomycin (Sal) inhibits TGF-beta 1-induced EMT by downregulating MMP-2 and MMP-9 through the AMPK/SIRT pathway, thereby blocking lung cancer cell migration and invasion.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tsui-Hwa Tseng, Huei-Jane Lee, Yean-Jang Lee, Ko-Chao Lee, Chien-Heng Shen, Hsing-Chun Kuo
Summary: Ailanthoidol (ATD) exhibits anti-liver cancer properties by inhibiting TGF-beta 1-induced migration, invasion, and colony formation in HepG2 hepatoblastoma cells, as well as suppressing the expression of integrin alpha 3, vimentin, N-cadherin, and MMP2; it acts against liver cancer progression by inhibiting the phosphorylation of p-38 mitogen-activated protein kinase (MAPK) and Smad 2.
Article
Toxicology
Sarah Zeitlmayr, Susanna Zierler, Claudia A. Staab-Weijnitz, Alexander Dietrich, Fabienne Geiger, F. David Horgen, Thomas Gudermann, Andreas Breit
Summary: Pulmonary fibrosis is a devastating disease characterized by excessive deposition of extracellular matrix proteins. TRPM7 has been identified as a novel supporter of TGF-beta 1 signaling in pulmonary fibroblasts, and inhibition of TRPM7 activity may provide a new therapeutic approach to control excessive ECM levels in pulmonary fibrosis.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Pharmacology & Pharmacy
Shirong Li, Anjian Xu, Yanmeng Li, Chunting Tan, Giuseppe La Regina, Romano Silvestri, Haoyan Wang, Wenjie Qi
Summary: The study demonstrated that RS4651 alleviates BLM-induced pulmonary fibrosis in mice by inhibiting the TGF-beta 1/SMAD signaling pathway. RS4651 administration decreased collagen deposition and alpha-SMA levels in the lungs of mice, while inhibiting proliferation and migration of MRC-5 cells, as well as alpha-SMA and pSMAD2 expression. RNA-sequencing data identified SMAD7 as the target gene, and further experiments showed that RS4651 upregulates SMAD7 expression to inhibit fibrosis progression.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Immunology
Sonia Kiran, Mousumi Mandal, Ahmed Rakib, Amandeep Bajwa, Udai P. Singh
Summary: This study investigates the role and mechanism of miR-10a-3p in adipose inflammation and adipogenesis using ex vivo and in vitro approaches. The findings suggest that miR-10a-3p mimic reduces the expression of inflammatory cells and cytokines, and induces the expression of FoxP3 in adipose tissue. In vitro experiments also show that miR-10a-3p mimic reduces the expression of inflammatory genes and lipid accumulation. This study provides a new opportunity for the development of miR-10a-3p as a novel therapeutic for adipose inflammation and its associated metabolic disorders.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Polymer Science
Jef Brebels, Arn Mignon
Summary: Flexor tendon injuries are common and take a long time to heal, with various complications. Current traditional repair strategies have limitations, but polymer-based constructs show promise in addressing these issues. This review aims to evaluate these strategies and improve the well-being of flexor tendon patients.
Review
Cell & Tissue Engineering
Hanyue Li, Shengyu Luo, Hongtao Li, Hongyu Pan, Li Jiang, Yixuan Chen, Hui Chen, Zhenhua Feng, Sen Li
Summary: We reviewed the biological factors that contribute to fetal tendon regeneration and how manipulation of these biochemical cues in the adult tendon healing process could achieve regeneration. The results showed that inflammation and TGF-beta level are the main elements involved in fetal tendon regeneration. Experimental manipulation of these biochemical cues in the adult tendon healing process demonstrated that although the blockade of TGF-beta 1, TGF-beta 2 and inflammation reduced scar tissue in adult tendon healing, this inhibition also destroyed the mechanical properties of the tendons. An effective alternative is regulating the specific downstream profibrotic effectors of both TGF-beta 1 and inflammation, which is preferable to those that completely inhibit these factors. Finally, TGF-beta 3 is a master regulator allowing a shift from adult scar healing to scarless healing, and the administration of TGF-beta 3 is a viable strategy to promote adult regenerative healing.
REGENERATIVE MEDICINE
(2023)
Article
Orthopedics
Rahul G. Alenchery, Raquel E. Ajalik, Kyle Jerreld, Firaol Midekksa, Sylvia Zhong, Bashar Alkatib, Hani A. Awad
Summary: This study found that transforming growth factor-beta (TGF-beta 1) induces plasminogen activator inhibitor 1 (PAI-1) to inhibit phosphatase and tensin homolog (PTEN), suggesting that PAI-1-induced adhesions involve phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin (mTOR) signaling. PAI-1 deletion blunted TGF-beta 1-induced myofibroblast activation in murine flexor tenocytes and increased the gene expression of Mmp-2 to confer protective effects against fibrosis. While TGF-beta 1 significantly reduced phosphorylation of PTEN in WT cells, PAI-1 deletion rescued the activation of PTEN.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Review
Critical Care Medicine
Hanyue Li, Shengyu Luo, Hao Wang, Yixuan Chen, Mingzhe Ding, Jingwei Lu, Li Jiang, Kexin Lyu, Shilin Huang, Houyin Shi, Hui Chen, Sen Li
Summary: Tendon injuries are a significant part of musculoskeletal diseases and have limited treatment options due to the lack of understanding of underlying molecular mechanisms. Transforming Growth Factor-beta 1 (TGF-beta 1) is an important profibrogenic factor in the tendon healing process, but blocking it does not effectively enhance tendon healing.
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2023)
Article
Endocrinology & Metabolism
Regis J. O'Keefe, Rocky S. Tuan, Nancy E. Lane, Frank Barry, Bruce A. Bunnell, Celine Colnot, Matthew T. Drake, Hicham Drissi, Lisa A. Fortier, Robert E. Guldberg, David G. Little, Mary F. Marshall, Jeremy J. Mao, Norimasa Nakamura, Pamela G. Robey, Vicki Rosen, David W. Rowe, Edward M. Schwarz
JOURNAL OF BONE AND MINERAL RESEARCH
(2020)
Article
Orthopedics
Katherine T. Best, Valentina Studentsova, Jessica E. Ackerman, Anne E. C. Nichols, Marlin Myers, Justin Cobb, Emma Knapp, Hani A. Awad, Alayna E. Loiselle
Summary: This study investigated the effects of tamoxifen on tendon homeostasis and healing in mice. The results showed that tamoxifen had no effect on tendon healing in male mice, while it significantly decreased Max load in female repairs. Additionally, tamoxifen treatment led to disruptions in tendon homeostasis in female mice compared to corn oil treatment.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Microbiology
Elysia A. Masters, Karen L. de Mesy Bentley, Ann Lindley Gill, Stephanie P. Hao, Chad A. Galloway, Alec T. Salminen, Diamond R. Guy, James L. McGrath, Hani A. Awad, Steven R. Gill, Edward M. Schwarz
Article
Biochemistry & Molecular Biology
Katherine T. Best, Anne E. C. Nichols, Emma Knapp, Warren C. Hammert, Constantinos Ketonis, Jennifer H. Jonason, Hani A. Awad, Alayna E. Loiselle
Article
Biophysics
Christine Massie, Emma Knapp, Keren Chen, Andrew J. Berger, Hani A. Awad
Summary: The study assessed bone fracture risk by measuring aBMD and fracture toughness using DXA, mCT, and Raman spectroscopy. It found that Raman spectroscopy significantly improved the prediction of fracture toughness, showing the potential to enhance diagnostic tools for assessing fragility fracture risk.
JOURNAL OF BIOMECHANICS
(2021)
Article
Orthopedics
Alexander S. Greenstein, Raymond E. Chen, Alexander M. Brown, Emma Knapp, Aaron Roberts, Hani A. Awad, Ilya Voloshin
Summary: Both single-row and double-row arthroscopic bony Bankart repair techniques resulted in damage to the humeral head articular cartilage in the cadaveric model used in this study. The double-row technique caused significantly more chondral damage compared to the single-row repair.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2021)
Article
Andrology
Taymour Mostafa, Abd El-Aziz I. El-Taweel, Laila Ahmed Rashed, Nayera A. M. Mohammed, Essam Mohamed Akl
Summary: Varicocele has been linked to male infertility, and improvements in sperm parameters post-varicocelectomy support this association. Cystatin C has been associated with cellular changes common in male infertility cases related to varicocele. This preliminary study found that seminal Cys C levels were significantly higher in infertile OAT men with varicocele compared to fertile men, and decreased post-operatively, suggesting a potential role of Cys C in varicocele-induced infertility.
Review
Obstetrics & Gynecology
Taymour Mostafa, Ibrahim Abdel-Hamid, Mai Taymour, Omar Ali
Summary: This systematic review examined the association between genetic factors and varicocele-related male infertility (VRMI). The findings suggest that there is no clear evidence of a strong association between specific genetic polymorphisms and VRMI. However, several studies identified a higher frequency of the polymorphic genotype for the rs1799983 polymorphism of the NOS3 gene in varicocele patients. Further efforts are needed to standardize reporting, explore complementary study designs, and utilize GWAS technology to replicate these early findings.
Article
Biology
Rubens Sautchuk, Brianna H. Kalicharan, Katherine Escalera-Rivera, Jennifer H. Jonason, George A. Porter, Hani A. Awad, Roman A. Eliseev
Summary: This study identifies BMP/Smad signaling as a transcriptional regulator of CypD expression and reveals the transcriptional repression of CypD during cell differentiation. The downregulation of CypD has a negative effect on osteogenesis.
Article
Infectious Diseases
Mikaeel Young, Danica J. Walsh, Elysia Masters, Vijay Singh Gondil, Emily Laskey, Michael Klaczko, Hani Awad, James McGrath, Edward M. Schwarz, Christian Melander, Paul M. Dunman
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a global healthcare concern. Recent studies have shown that penicillin binding protein 4 (PBP4) is also involved in MRSA resistance. Inhibitors targeting PBP4 may reverse methicillin resistance and inhibit MRSA's ability to cause chronic osteomyelitis.
Article
Endocrinology & Metabolism
Rubens Sautchuk Jr, Chen Yu, Matthew McArthur, Christine Massie, Paul S. S. Brookes, George A. A. Porter Jr, Hani Awad, Roman A. A. Eliseev
Summary: CypD/MPTP overactivation plays an important role in bone aging, and overexpression of CypD impairs bone cell energy metabolism and function. Spatial-dependent and sex-dependent studies found that CypD GOF leads to a decrease in oxidative phosphorylation levels, increased oxidative stress, and decreased bone organic matrix content, ultimately resulting in bone aging. This study confirms the important pathological mechanism of CypD/MPTP overactivation in bone aging and osteoporosis.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Orthopedics
Spencer P. Lake, Jess G. Snedeker, Vincent M. Wang, Hani Awad, Hazel R. C. Screen, Stavros Thomopoulos
Summary: Characterizing the mechanical properties of tendons is crucial for assessing their health and the effectiveness of injury treatments. This paper provides guidelines for nonexperts on methodological considerations, testing protocols, and outcome measures for tendon mechanical testing.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Andrology
Mohamed Wael Ragab, Mina Saad, Zeinab Nour, Hamed Abdallah Hamed, Taymour Mostafa, Ahmed M. El-Guindi
Summary: This study aimed to evaluate the predictive role of seminal testis expressed protein (TEX101) in sperm recovery in infertile men with nonobstructive azoospermia (NOA) after microsurgical varicocelectomy. The results showed that NOA patients with higher preoperative TEX101 levels had successful sperm recovery in their semen. Additionally, NOA patients without seminal sperm recovery had higher levels of serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Therefore, preoperative seminal TEX101 may serve as a biomarker for predicting sperm recovery in NOA patients with varicocele.
Review
Andrology
Ramy Abou Ghayda, Rossella Cannarella, Aldo E. Calogero, Rupin Shah, Amarnath Rambhatla, Wael Zohdy, Parviz Kavoussi, Tomer Avidor-Reiss, Florence Boitrelle, Taymour Mostafa, Ramadan Saleh, Tuncay Toprak, Ponco Birowo, Gianmaria Salvio, Gokhan Calik, Shinnosuke Kuroda, Raneen Sawaid Kaiyal, Imad Ziouziou, Andrea Crafa, Nguyen Ho Vinh Phuoc, Giorgio I. Russo, Damayanthi Durairajanayagam, Manaf Al-Hashimi, Taha Abo-Almagd Abdel-Meguid Hamoda, Germar-Michael Pinggera, Ricky Adriansjah, Israel Maldonado Rosas, Mohamed Arafa, Eric Chung, Widi Atmoko, Lucia Rocco, Haocheng Lin, Eric Huyghe, Priyank Kothari, Jesus Fernando Solorzano Vazquez, Fotios Dimitriadis, Nicolas Garrido, Sheryl Homa, Marco Falcone, Marjan Sabbaghian, Hussein Kandil, Edmund Ko, Marlon Martinez, Quang Nguyen, Ahmed M. Harraz, Ege Can Serefoglu, Vilvapathy Senguttuvan Karthikeyan, Dung Mai BaTien, Sunil Jindal, Sava Micic, Marina Bellavia, Hamed Alali, Nazim Gherabi, Sheena Lewis, Hyun Jun Park, Mara Simopoulou, Hassan Sallam, Liliana Ramirez, Giovanni Colpi, Ashok Agarwal
Summary: Artificial intelligence (AI) has made significant progress in the field of medicine and has been applied to various areas. Advances in computer science, medical informatics, robotics, and personalized medicine have facilitated AI's role in modern healthcare. In andrology and reproductive medicine, AI applications such as machine learning, artificial neural networks, and deep learning have shown great potential in diagnosing and treating male infertility, as well as improving patient care.
WORLD JOURNAL OF MENS HEALTH
(2023)
Article
Orthopedics
Alexander S. Greenstein, Raymond E. Chen, Alexander M. Brown, Emma Knapp, Aaron Roberts, Hani A. Awad, Ilya Voloshin
Summary: In this study, the impact of arthroscopic bony Bankart repair techniques on humeral head articular cartilage damage was investigated using a cadaveric model. The results showed that the double-row repair technique resulted in significantly higher chondral damage compared to the single-row repair technique.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2021)
