Article
Cell Biology
Kexin Zhang, Agata Tarczykowska, Deepesh Kumar Gupta, Devon F. Pendlebury, Cassandra Zuckerman, Jayakrishnan Nandakumar, Hiroki Shibuya
Summary: The MYB domain of the meiosis-specific telomere-binding protein TERB1 regulates the enrichment of cohesin and promotes the remodeling of axial elements, suppressing meiotic telomere erosion.
Article
Gastroenterology & Hepatology
Kapil K. Upadhyay, Xiaomeng Du, Yanhua Chen, Brandon Buscher, Vincent L. Chen, Antonino Oliveri, Raymond Zhao, Elizabeth K. Speliotes, Graham F. Brady
Summary: This study found that a common variant in nuclear envelope-related genes, rs6461378, is associated with NAFLD and metabolic disease. This variant leads to increased degradation of proteins and results in insulin resistance and lipid accumulation.
JOURNAL OF HEPATOLOGY
(2023)
Article
Cell Biology
Manickam Gurusaran, Jelle J. Biemans, Christopher W. Wood, Owen R. Davies
Summary: The LINC complex is crucial for maintaining nuclear morphology and transmitting forces in meiosis and somatic cells. The crystal structure of SUN1's luminal region provides insight into its composition and suggests the presence of flexible domains and potential domain-swap interactions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Shi Jiao, Chuanchuan Li, Fenghua Guo, Jinjin Zhang, Hui Zhang, Zhifa Cao, Wenjia Wang, Wenbo Bu, Mobin Lin, Junhong Lue, Zhaocai Zhou
Summary: Alteration of the size and stiffness of the nucleus triggered by environmental cues is important for eukaryotic cell fate and function. This study identifies the nuclear envelope proteins SUN1/2 as mechano-regulators of the nucleus during M1 polarization, and demonstrates that SUN1/2 proteins act as mechano-regulators to remodel the nucleus and chromatin for M1 polarization of the macrophage in response to inflammatory cues.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yanyan Chen, Yan Wang, Juan Chen, Wu Zuo, Yong Fan, Sijia Huang, Yongmei Liu, Guangming Chen, Qing Li, Jinsong Li, Jian Wu, Qian Bian, Chenhui Huang, Ming Lei
Summary: The interaction between telomeres and the LINC complex, specifically between SUN1 and SPDYA, is crucial for homologous pairing during meiosis prophase I. This interaction is essential for the formation of a ring-shaped telomere supramolecular architecture at the nuclear envelope, which is critical for efficient meiotic progression.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Hanumanthu B. D. Prasada Rao, Takeshi Sato, Kiran Challa, Yurika Fujita, Miki Shinohara, Akira Shinohara
Summary: During meiosis, CDK and DDK regulate the dynamics of Mps3 on the nuclear envelope by mediating the formation of Mps3 clusters and telomere clustering. The luminal region of Mps3 plays a crucial role in the meiosis-specific localisation of Mps3 on the nuclear envelope. Phosphorylation-dependent regulation of Mps3 localisation on the nuclear envelope is essential for meiotic chromosome motion and nuclear envelope remodelling.
Article
Cell Biology
Fernando Hernandez-Sanchez, Leonardo Peraza-Reyes
Summary: Eukaryotic cell development involves precise regulation of organelles to adapt to changing requirements. Meiotic development involves the formation and dynamics of distinct intracellular membrane compartments, and plays important roles in organelle segregation, quality control, and cell rejuvenation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Edgar del Llano, Rajan Iyyappan, Daria Aleshkina, Tomas Masek, Michal Dvoran, Zongliang Jiang, Martin Pospisek, Michal Kubelka, Andrej Susor
Summary: In mammalian females, the maturation and development of oocytes are regulated by various factors, including the maturation-promoting factor (MPF) complex and serum-glucocorticoid kinase proteins (SGK1). This study demonstrates that SGK1 is expressed in mouse oocytes and its activation promotes CDK1 activation, leading to the development and maturation of oocytes.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Diana Viegas, Catia D. Pereira, Filipa Martins, Tiago Mateus, Odete A. B. da Cruz e Silva, Maria Teresa Herdeiro, Sandra Rebelo
Summary: DM1 is a hereditary and multisystemic disease characterized by altered nuclear profile and protein levels in patient-derived cells, indicating the relevance of nuclear envelope dysfunction in the pathogenesis of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Jian Li, Hao-Ya Chang, Zi-Yun Yi, Chun-Hui Zhang, Qing-Yuan Sun, Wei-Ping Qian
Summary: Studies on mammalian oocytes have shown that CDK2 activity plays a crucial role in the proper dynamics of spindle and chromosomes, with CDK2 inhibition leading to continuous SAC activation and subsequent inactivation of APC/C activity.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ei Leen Leong, Nyein Thet Khaing, Bruno Cadot, Wei Liang Hong, Serguei Kozlov, Hendrikje Werner, Esther Sook Min Wong, Colin L. Stewart, Brian Burke, Yin Loon Lee
Summary: Mutations in LMNA gene cause laminopathies, which are diseases affecting striated muscle and other tissues. This study identifies Nesprin-1 LINC complexes as important regulators of nuclear positioning and morphology in striated muscle cells. Disruption of the KASH domain of Nesprin-1 reduces the impact of microtubule cytoskeleton on the nucleus and suppresses Lmna-linked cardiac pathology. This suggests that Nesprin-1 LINC complexes could be potential therapeutic targets for striated muscle laminopathies.
HUMAN MOLECULAR GENETICS
(2022)
Article
Cell Biology
Tanita Frey, Tomonari Murakami, Koichiro Maki, Takumi Kawaue, Naoki Tani, Ayaka Sugai, Naotaka Nakazawa, Kei-ichiro Ishiguro, Taiji Adachi, Mineko Kengaku, Kenichi Ohki, Yukiko Gotoh, Yusuke Kishi
Summary: Neurons decline in functionality with age and this is associated with neurodegenerative diseases. Our study found that aged nuclei already exhibit infolding even before stimulation, indicating reduced dynamics of nuclear morphology. Stimulated young nuclei showed reversible nuclear infolding, while aged nuclei were stiffer and had decreased morphological dynamics. Our findings reveal temporal changes in nuclear shape and age-related loss of dynamics upon external stimulation.
Article
Biochemistry & Molecular Biology
Neil Hattersley, Aleesa J. Schlientz, Bram Prevo, Karen Oegema, Arshad Desai
Summary: The nucleoporin MEL-28 and CENP-CHCP-4 act in parallel to recruit outer kinetochore components to oocyte meiotic chromosomes, and they independently link chromosomes to spindle microtubules.
Review
Genetics & Heredity
Hyung Jun Kim, Chenshu Liu, Abby F. Dernburg
Summary: This article summarizes the connections established between chromosomes and cytoplasmic motors via a nuclear envelope bridge during the early meiotic prophase, known as a LINC complex, and the promotion of chromosome and nuclear motions. While the molecular architecture of these connections has been illuminated in diverse organisms, important questions remain regarding their contributions to meiotic processes.
Article
Cell Biology
Anne F. J. Janssen, Sophia Y. Breusegem, Delphine Larrieu
Summary: Nuclear morphology abnormalities have significant impact on cellular functioning and genomic integrity, making quantitative measurement of nuclear shape crucial. This article provides an overview of various nuclear abnormalities and reviews current image-based quantification methods. New pipelines for quantitative analysis of nuclear shape are also introduced. Quantitative analyses of nuclear aberrations and shape have wide applications in assessing cancer cell anomalies and studying nucleus deformability under stress.
Biographical-Item
Biochemistry & Molecular Biology
Carlos Garcia de la Vega, Rocio Gomez, Jesus Page, Maria Teresa Parra, Juan Luis Santos, Jose A. Suja, Alberto Viera
Article
Biochemistry & Molecular Biology
Ana Gil-Fernandez, Sergey Matveevsky, Marta Martin-Ruiz, Marta Ribagorda, Maria Teresa Parra, Alberto Viera, Julio S. Rufas, Oxana Kolomiets, Irina Bakloushinskaya, Jesus Page
Summary: Sex determination in mammals is typically based on XX in females and XY in males. However, Ellobius tancrei deviates from this norm, with both males and females having a pair of XX chromosomes that exhibit differential behavior in meiosis. While the male meiosis shows limited synapsis between the X chromosomes, leaving the central region completely unsynapsed, female meiosis experiences delayed synapsis in the central region. Recombination in the sex chromosomes is restricted in both sexes, with male meiosis showing differentiation of one of the sex chromosomes into a neo-Y and female meiosis lacking recombination in the central X chromosome region. These findings provide insights into the origin and evolution of sex chromosomes.
Article
Genetics & Heredity
Ana Gil-Fernandez, Marta Ribagorda, Marta Martin-Ruiz, Pablo Lopez-Jimenez, Tamara Laguna, Rocio Gomez, Maria Teresa Parra, Alberto Viera, Frederic Veyrunes, Jesus Page
Summary: X and Y chromosomes in mammals differ in size and gene content due to an evolutionary process, with the Y chromosome undergoing differentiation and degeneration. The pseudoautosomal region (PAR) allows partial synapsis and reciprocal recombination of the sex chromosomes during meiosis, ensuring their segregation. The African pygmy mouse Mus mattheyi has completely differentiated sex chromosomes, with delayed pairing and unique protein dynamics, suggesting potential impact on sex chromosome divergence and transmission.
Article
Cell Biology
Roberto de la Fuente, Florencia Pratto, Abrahan Hernandez-Hernandez, Marcia Manterola, Pablo Lopez-Jimenez, Rocio Gomez, Alberto Viera, Maria Teresa Parra, Anna Kouznetsova, R. Daniel Camerini-Otero, Jesus Page
Summary: This study investigates the impact of disruptions in recombination and synapsis on the dynamics of epigenetic markers and transcriptional regulation during meiosis in knockout mice. Results show that impairment of recombination and synapsis alters the epigenetic marks and transcriptional regulation, suggesting a crucial role in the impairment of meiosis progression in mammals.
Article
Genetics & Heredity
Alberto Viera, Maria Teresa Parra, Sara Arevalo, Carlos Garcia de la Vega, Juan Luis Santos, Jesus Page
Summary: The regulation of transcription during meiosis in Eyprepocnemis plorans differs from the canonical pattern found in mammals, with the X chromosome remaining inactive throughout meiosis. X chromosome inactivation is likely preceded by a process of heterochromatinization before the initiation of meiosis.
Article
Cell Biology
Ines Berenguer, Pablo Lopez-Jimenez, Irene Mena, Alberto Viera, Jesus Page, Jose Gonzalez-Martinez, Carolina Maestre, Marcos Malumbres, Jose A. Suja, Rocio Gomez
Summary: The haspin-H3T3ph-chromosomal passenger complex (CPC) pathway is crucial for the regulation of centromeres during mammalian male meiosis, controlling chromosome segregation and spindle attachment. This study provides new insights into the functions of haspin kinase and the H3T3ph histone mark in meiotic centromere regulation.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Javier Galan-Martinez, Ines Berenguer, Ma del Carmen Maza, Konstantinos Stamatakis, Nuria Girones, Manuel Fresno
Summary: TCFL5 plays a crucial role in spermatogenesis by ensuring the progression of meiosis and the maturation of sperm cells.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Annette Graeve, Joshua Huster, Deria Goerl, Ioanna Ioannidou, Rocio Gomez, Linda C. Weiss
Summary: The cellular mechanisms underlying morphological defense expression in Daphnia, particularly the development of defensive crests, have been investigated. The study found that cell division is delayed in favor of cell growth, leading to crest formation. The defense development starts in the ventral region and extends towards the cranial and dorsal directions. These cellular changes begin as early as 2 hours after predator exposure.
Article
Cell Biology
Pablo Lopez-Jimenez, Sara Perez-Martin, Ines Hidalgo, Francesc R. Garcia-Gonzalo, Jesus Page, Rocio Gomez
Summary: Cilia play important roles in the airways and reproductive system as motile cilia beat to displace extracellular fluids, while primary cilia function as sensory organelles detecting signals from the external environment. Dysfunction of cilia is associated with genetic diseases and some types of cancer. In mouse meiosis, solitary cilia were found in zygotene spermatocytes, suggesting a potential sensory role affecting cyst function during prophase I.
Article
Cell Biology
Rocio Gomez, Alberto Viera, Tania Moreno-Marmol, Ines Berenguer, Andrea Guajardo-Grence, Attila Toth, Maria Teresa Parra, Jose A. Suja
Summary: By inhibiting the kinase activity of PLK1, our study revealed that PLK1 plays crucial roles in mammalian male meiosis, including the disassembly of SYCP3 and HORMAD1 from the synaptonemal complex and the recruitment of these proteins to the inner centromere. Moreover, PLK1 regulates the assembly of the inner centromere by controlling the recruitment of shugoshin SGO2 and Aurora B/C and Borealin. Overall, our results demonstrate that PLK1 is a master regulator of the late prophase I/metaphase I transition in mouse spermatocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
Marta Carus-Cadavieco, Ines Berenguer Lopez, Alba Montoro Canelo, Miguel A. Serrano-Lope, Sandra Gonzalez-de la Fuente, Begona Aguado, Alba Fernandez-Rodrigo, Takaomi C. Saido, Ana Frank Garcia, Cesar Venero, Jose A. Esteban, Francesc Guix, Carlos G. Dotti
Summary: This study tested the hypothesis that individuals with type 2 diabetes (T2DM) will only develop dementia if they have a genetic predisposition to neurodegenerative disease. To support this concept, T2DM was induced in middle-aged hAPP NL/F mice, a preclinical model of Alzheimer's disease. The results showed that T2DM led to more severe behavioral, electrophysiological, and structural changes in these mice compared to wild-type mice. Mechanistically, the deficits were not caused by increased levels of toxic A beta or neuroinflammation, but by reduced gamma-secretase activity, decreased levels of synaptic proteins, and elevated tau phosphorylation. RNA-seq analysis suggested that hAPP NL/F mice may be more susceptible to T2DM due to defects in trans-membrane transport. These findings confirm the importance of genetic background in the severity of cognitive disorders in individuals with T2DM and suggest the inhibition of gamma-secretase activity as one of the underlying mechanisms.
LIFE SCIENCE ALLIANCE
(2023)