Article
Endocrinology & Metabolism
Mengbo Zhu, Wenbin Xu, Jiuzhou Jiang, Yining Wang, Yanjing Guo, Ruijia Yang, Yaqiong Chang, Bin Zhao, Zhenyu Wang, Jianfeng Zhang, Te Wang, Liqin Shangguan, Shaowei Wang
Summary: The study found that peiminine can inhibit the differentiation and function of OCs by regulating the levels of specific genes and proteins, with potential targets including NF-kappa B and ERK1/2 signaling pathways. In an animal model, peiminine can alleviate bone loss in ovariectomized mice.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Chemistry, Medicinal
Hai-Wei Chen, Chi-Fen Chen, Pei-Rong Lee, Shuenn-Jium Yiin, Jia-Yun Liang, Jue-Liang Hsu, Chi- Chang, Chi-Liang Chern
Summary: 2,5-dihydroxyacetophenone (DHAP), a phenolic compound in Ganoderma bambusicola, inhibits osteoclastogenesis by inhibiting the NFATc1 signaling pathway.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Immunology
Delong Chen, Feifan Chu, Gangyu Zhang, Qingqing Wang, Ying Li, Meng Zhang, Qi He, Junzheng Yang, Haibin Wang, Ping Sun, Jiake Xu, Peng Chen
Summary: The natural compound 12-deoxyphorbol 13-acetate (DPA) was found to inhibit osteoclast differentiation and function, potentially serving as a promising treatment for osteoporosis. Mechanistically, DPA suppressed RANKL-induced downstream signaling pathways and showed efficacy in an osteoporosis mice model.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mingchao Jin, Jiangbo Nie, Juli Zhu, Jing Li, Tianshun Fang, Juntao Xu, Xuesheng Jiang, Zhuo Chen, Jianyou Li, Fengfeng Wu
Summary: This study demonstrated for the first time that acacetin inhibited osteoclastogenesis, bone resorption activity, and related gene expression by modulating multiple signaling pathways, suggesting its potential therapeutic effects for preventing osteoporosis and other osteolytic disorders.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Gang Wang, Chao Ma, Kai Chen, Ziyi Wang, Heng Qiu, Delong Chen, Jianbo He, Cheng Zhang, Ding Guo, Boyong Lai, Shuangxiao Zhang, Linfeng Huang, Fan Yang, Jinbo Yuan, Leilei Chen, Wei He, Jiake Xu
Summary: This study demonstrates that cycloastragenol (CAG) inhibits the formation and function of osteoclasts by regulating intracellular signaling pathways. It has potential as an alternative treatment for osteoporosis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jirong Wang, Chengyun Xu, Jing Zhang, Yizhong Bao, Ying Tang, Xiaoling Lv, Bo Ma, Ximei Wu, Genxiang Mao
Summary: RhoA protein plays a crucial role in regulating the cytoskeletal architecture of osteoclasts and bone resorption activity. Deletion of RhoA leads to severe osteopetrosis phenotype and suppression of bone resorption, which is mediated by inhibiting Akt-mTOR-NFATc1 signaling during osteoclast differentiation. Activation of RhoA is associated with enhanced osteoclast activity and the development of osteoporotic bone phenotype. The absence of RhoA in osteoclast precursors in mice prevents OVX-induced bone loss.
MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Xin Liu, Xiaosa Xu, Jinping Li, Liying Shi, Ying Zeng, Siyuan Tang, Wei Liu, Lujuan Jia, Yuhong Li, Jie Zhang
Summary: In this study, it was discovered that isobavachalcone, derived from the traditional Chinese herb Psoralea corylifolia Linn, can inhibit osteoclastogenesis and bone resorption. The mechanism involves the suppression of NF-κB activation and downregulation of osteoclastic-specific genes and proteins. This suggests that isobavachalcone may have potential as a therapeutic compound for the treatment of osteoporosis and other metabolic bone diseases.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Immunology
Jiachao Guo, Ranyue Ren, Zhou Guo, Kai Sun, Jinpeng He, Jingfan Shao, Xiaolin Wang
Summary: Osteoporosis is a systemic and endocrine bone disorder characterized by decreased bone mineral density, compromised bone strength, and destruction of trabecular structure. The excessive osteoclastogenesis and bone erosion contribute to the progression of the disease. However, existing medications have poor adherence and adverse reactions, highlighting the urgent need for novel therapies for osteoporosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Riko Kitazawa, Ryuma Haraguchi, Yukihiro Kohara, Sohei Kitazawa
Summary: The RANK receptor regulates the differentiation and function of osteoclasts through direct binding of NFATc1. The RANKL signal positively regulates RANK transcription, promoting accelerated terminal differentiation of osteoclast precursors to mature osteoclasts.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Gang Wang, Kai Chen, Chao Ma, Chao Wang, Delong Chen, Jianbo He, Yuhao Liu, Tao Jiang, Jinbo Yuan, Leilei Chen, Wei He, Jiake Xu
Summary: RA is a natural compound that shows potential therapeutic effects by inhibiting osteoclast differentiation and reducing bone loss. It suppresses osteoclastogenesis without causing cell toxicity, making it a promising treatment option for skeletal diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuria Jang, Hong Moon Sohn, Young Jong Ko, Hoon Hyun, Wonbong Lim
Summary: The mutant RANKL demonstrated the ability to inhibit osteoclastogenesis by competitively inhibiting RANKL, serving as a potential therapeutic agent for osteoporosis with fewer toxic side effects compared to traditional treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Yin Cheng, Haixia Liu, Jing Li, Yujie Ma, Changheng Song, Yuhan Wang, Pei Li, Yanjing Chen, Zhiguo Zhang
Summary: Monascin was found to inhibit osteoclastogenesis in RANKL-induced RAW264.7 cells by suppressing MAPKs signaling pathways.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Yuxin Wang, Xiang Li, Shengji Zhou, Jiarui Li, Yi Zhu, Quan Wang, Fengchao Zhao
Summary: Osteoporosis is a common disorder characterized by greater bone resorption than formation, leading to decreased bone mass and increased fracture risk. Inhibiting osteoclast formation and resorption ability has been found to be effective in treating and preventing osteoporosis. This study is the first to explore the role of the mitochondrial calcium uniporter (MCU) and its inhibitor ruthenium red (RR) in bone metabolism and elucidate its specific mechanism in inhibiting osteoclast formation and promoting osteogenesis in the treatment of osteoporosis.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Immunology
Feng Long, Runfeng Chen, Yuangang Su, Jiamin Liang, Yansi Xian, Fan Yang, Haoyu Lian, Jiake Xu, Jinmin Zhao, Qian Liu
Summary: This study found that epoxymicheliolide (EMCL) may have potential effects in treating osteoporosis. EMCL inhibits the phosphorylation of ERK1/2 and the entry of NFATc1 into the nucleus, reducing osteoclast production and bone resorption.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yinglong Xu, Dezhi Song, Xixi Lin, Hui Peng, Yuangang Su, Jiamin Liang, Na Hai, Jinmin Zhao, Qian Liu
Summary: Corylifol A can prevent estrogen-deficiency-induced bone loss by inhibiting osteoclastogenesis and intracellular ROS levels. This study also found that Corylifol A has no effect on cortical bone in ovariectomized mice.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Y. Shi, W. Jones, W. Beatty, Q. Tan, R. P. Mecham, H. Kumra, D. P. Reinhardt, M. A. Gibson, M. A. Reilly, J. Rodriguez, S. Bassnett
Summary: LTBP-2 is essential for the strength and longevity of zonular fibers, with its absence leading to fiber rupture and ectopia lentis in adulthood. The mechanical properties of zonule are significantly altered in LTBP-2-deficient mice, affecting their ability to withstand forces generated during eye movements.
Article
Biochemistry & Molecular Biology
Zerina Balic, Saurav Misra, Belinda Willard, Dieter P. Reinhardt, Suneel S. Apte, Dirk Hubmacher
Summary: ADAMTS proteases play important roles in the biosynthesis and breakdown of ECM molecules, with alternative splicing playing a significant role in regulating their proteolytic activity and cellular localization. This study characterizes the impact of alternative splicing on ADAMTS17, revealing two novel splice variants that affect protease activity through structural changes.
Article
Ophthalmology
Rodrigo Barbosa de Souza, Isabela Gerdes Gyuricza, Luara Lucena Cassiano, Luis Ernesto Farinha-Arcieri, Ana Maria Alvim Liberatore, Sheila Schuindt do Carmo, Waldir Caldeira, Marcio Cruz, Alberto F. Ribeiro, Roberto Carlos Tedesco, Dieter P. Reinhardt, Ricardo Smith, Ivan Hong Jun Koh, Lygia Pereira
Summary: A study found that a heterozygous dominant-negative mutation in the Fbn1 gene in mice leads to disruption of microfibrils in the zonule, causing lens dislocation and enlargement of the anterior chamber.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Rong-Mo Zhang, Karina A. Zeyer, Nadine Odenthal, Yiyun Zhang, Dieter P. Reinhardt
Summary: The study revealed that fibrillin-1 regulates fibroblast proliferation by controlling miR-1208, involving the c-Src kinase and JNK signaling. miR-1208 targets ERK2 and MEK1 mRNAs, affecting the phosphorylation levels of ERK1/2 and MEK1/2 proteins. This novel outside-in signaling mechanism explains how fibrillin-1 RGD-cell binding regulates fibroblast proliferation.
Article
Biochemistry & Molecular Biology
Jeremy Boizot, Melaine Minville-Walz, Dieter Peter Reinhardt, Marielle Bouschbacher, Pascal Sommer, Dominique Sigaudo-Roussel, Romain Debret
Summary: Most chronic wounds are affected by hypoxia, but studies about wound healing are usually conducted under normal oxygen conditions. This study found that hypoxia inhibits the synthesis of elastic fibers and specifically affects the expression of fibrillin-2 and lysyl oxidase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Rong-Mo Zhang, Kerstin Tiedemann, Muthu L. Muthu, Neha E. H. Dinesh, Svetlana Komarova, Bhama Ramkhelawon, Dieter P. Reinhardt
Summary: This study demonstrates that miR-122 in the aortic wall inhibits inflammatory responses and matrix remodeling in thoracic aortic aneurysms (TAA) associated with Marfan syndrome. Deficient fibrillin-1-cell interaction and hypoxia suppress miR-122 expression, leading to elevated pro-inflammatory cytokines and matrix metalloproteinases, as well as elastin fragmentation and aortic dilation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Muthu L. Muthu, Kerstin Tiedemann, Julie Fradette, Svetlana Komarova, Dieter P. Reinhardt
Summary: This study demonstrates the important role of Fibrillin-1 in adipocyte development and adipose tissue homeostasis. Using mouse models and cell culture methods, the study shows that Fibrillin-1 deficiency leads to increased adipocyte numbers, obesity, insulin resistance, and dyslipidemia. Additionally, the study reveals that Fibrillin-1 deficiency promotes adipogenic gene expression, further enhancing adipocyte differentiation and expansion.
Article
Biology
Timothy J. Mead, Daniel R. Martin, Lauren W. Wang, Stuart A. Cain, Cagri Gulec, Elisabeth Cahill, Joseph Mauch, Dieter Reinhardt, Cecilia Lo, Clair Baldock, Suneel S. Apte, Ernestina Schipani
Summary: This study reveals a proteostatic mechanism contributing to the reduction of fibrillin-2 and dominance of fibrillin-1 during postnatal development. The lack of ADAMTS6 and ADAMTS10 leads to excess fibrillin-2 and impairs skeletal development and signaling. The protease-substrate relationship between ADAMTS6 and fibrillin-2 is established.
Review
Cell Biology
Neha E. H. Dinesh, Philippe M. Campeau, Dieter P. Reinhardt
Summary: The extracellular matrix is crucial for regulating cell development, with fibronectin playing a key role in determining cell fate and function during skeletal development. However, the specific role and importance of fibronectin isoforms in bone and cartilage physiology remains poorly understood. This review provides an overview of the role of fibronectin isoforms in skeletal development and discusses the consequences of mutations in fibronectin on disease development. The review also explores potential therapeutic and clinical implications of fibronectin-related molecular pathways.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Cell Biology
Kerstin Tiedemann, Muthu L. Muthu, Dieter P. Reinhardt, Svetlana Komarova
Summary: Gene mutations in fibrillin-1 can cause metabolic adaptations to different diets, leading to obesity and diabetes in male MFS mice. Female MFS mice, however, are less affected by high-fat diets.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Florian Alonso, Yuechao Dong, Ling Li, Tiya Jahjah, Jean-William Dupuy, Isabelle Fremaux, Dieter P. Reinhardt, Elisabeth Genot
Summary: Fibrillin-1 is a crucial factor in angiogenesis and its mutation disrupts blood vessel formation. Fibrillin-1 is found at the angiogenic front and regulates signaling pathways to promote endothelial cell growth. Administration of a C-terminal fragment of fibrillin-1 corrects the defects in mutant mice.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cardiac & Cardiovascular Systems
Cori Lau, Muthu L. Muthu, Iram Fatima Siddiqui, Ling Li, Dieter P. Reinhardt
Summary: This study reveals a specific sexual dimorphism in a mouse model of Marfan syndrome, with a high-fat diet exerting an explicit protective effect on severity of aortic disease in female mice. These preclinical data may be useful for developing nutritional recommendations for individuals with Marfan syndrome in the longer term.
CANADIAN JOURNAL OF CARDIOLOGY
(2023)
Article
Medicine, General & Internal
Rebekah Jaung, Lynne Soon-Chean Park, Joohyun Justine Park, David Tokiharu Mayeda, Changzoo Song
Summary: This study examines the experiences of racism among New Zealand Asians and its impact on their life satisfaction during the COVID-19 pandemic.
NEW ZEALAND MEDICAL JOURNAL
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Rodrigo Barbosa Souza, Dieter P. Reinhardt, Ivan H. Koh, Lygia D. Pereira
Correction
Veterinary Sciences
Etienne Cote, Rong-Mo Zhang, Nicole Kaiser, Dieter P. Reinhardt, Chelsea K. Martin
VETERINARY QUARTERLY
(2021)