Article
Biology
Qianyun Mei, Qi Yu, Xin Li, Jianguo Chen, Xilan Yu
Summary: Histone loss during yeast aging compromises telomere silencing by promoting Sir2 degradation through autophagy. Reduction of core histones enhances autophagy pathway, further accelerating autophagy-mediated Sir2 degradation. Screening of histone mutants and one histone modification identifies regulators of telomere silencing via modulation of core histones-autophagy-Sir2 axis.
LIFE SCIENCE ALLIANCE
(2023)
Article
Multidisciplinary Sciences
Anne Meldgaard Hansen, Ying Ge, Mikkel Bruhn Schuster, Sachin Pundhir, Janus Schou Jakobsen, Adrija Kalvisa, Marta Cecylia Tapia, Sandra Gordon, Francesca Ambri, Frederik Otzen Bagger, Deo Pandey, Kristian Helin, Bo Torben Porse
Summary: The study reveals that H3K9me2 is crucial for the proliferation of AML and its loss leads to activation of the interferon pathway. Mechanistically, destabilization of a complex involving SUV39H1, G9A, and GLP is responsible for this activation.
Article
Biochemistry & Molecular Biology
Ryo Maeda, Makoto Tachibana
Summary: This study demonstrates that HP1 not only recognizes and spreads the epigenetic mark H3K9me2/3, but also plays a role in heterochromatin organization by regulating the protein stability of H3K9 methyltransferases and demethylases.
Article
Mycology
Ruilin Sun, Meifang Wen, Lianghuan Wu, Huahui Lan, Jun Yuan, Shihua Wang
Summary: Aspergillus flavus is a common filamentous fungus that produces the highly toxic compound aflatoxin. The study used Aspergillus flavus as a model organism to investigate the role of acetyltransferase gene rtt109, finding that it plays a crucial role in growth, conidium formation, sclerotia generation, aflatoxin synthesis, environmental stress response, and infection regulation in A. flavus.
Article
Parasitology
Francisco Alejandro Lagunas-Rangel, Maria Luisa Bazan-Tejeda, Rosa Maria Bermudez-Cruz
Summary: Giardia duodenalis is a parasite that causes numerous diarrheal diseases worldwide. Despite needing fewer components than other eukaryotes, Giardia still contains core histones and epigenetic marks, which suggest a control mechanism for gene expression.
Article
Biology
Kei Fukuda, Chikako Shimura, Hisashi Miura, Akie Tanigawa, Takehiro Suzuki, Naoshi Dohmae, Ichiro Hiratani, Yoichi Shinkai
Summary: The study reveals that H3K9me2 in mouse embryonic stem cells is regulated by multiple MTases, with different MTases controlling H3K9me2 and transcription in the A and B compartments. Additionally, decreased H3K9me2 is associated with a more active compartmental state and transcriptional activation, contributing to the setting of inactive compartments. This suggests that H3K9 methyltransferases play a crucial role in maintaining genome organization in transcriptionally inactive compartments.
COMMUNICATIONS BIOLOGY
(2021)
Article
Neurosciences
Rafael Alcala-Vida, Caroline Lotz, Baptiste Brule, Jonathan Seguin, Charles Decraene, Ali Awada, Aurelie Bombardier, Brigitte Cosquer, Anne Pereira de Vasconcelos, Emmanuel Brouillet, Jean-Christophe Cassel, Anne-Laurence Boutillier, Karine Merienne
Summary: This study reveals the molecular mechanisms underlying cognitive deficits in Huntington's disease, including altered activity-dependent epigenetic gene reprogramming and compromised H3K9 acetylation and TGF beta signaling in the striatum.
PROGRESS IN NEUROBIOLOGY
(2022)
Article
Cell & Tissue Engineering
Lixia Dong, Huaqi Liao, Linchun Zhao, Jingnan Wang, Congcong Wang, Bowen Wang, Yanqi Sun, Lijun Xu, Yin Xia, Shizhang Ling, Xin Lou, Jinzhong Qin
Summary: The interaction between H3K9 methylation writers and readers is crucial for maintaining the identity of embryonic stem cells.
Article
Biochemistry & Molecular Biology
Tao Fan, Chu Xiao, Hengchang Liu, Yu Liu, Liyu Wang, He Tian, Chunxiang Li, Jie He
Summary: CFP1, as a non-catalytic component of COMPASS, promotes tumorigenesis through genomic histone methylation reprogramming, and has a positive effect on the proliferation, migration and invasion of lung adenocarcinoma cells. It also has an adverse impact on cancer prognosis by affecting the transcriptome and signaling pathways.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Anne Caroline Mascarenhas dos Santos, Alexander Thomas Julian, Pingdong Liang, Oscar Juarez, Jean-Francois Pombert
Summary: In this study, the complete genomes of three human-infecting Encephalitozoon spp. were sequenced, revealing that their chromosomes are composed of TTAGG 5-mer telomeric repeats and hypermethylated ribosomal RNA gene loci adjacent to telomeres. The presence of genes involved in telomere maintenance, epigenetic regulation, and heterochromatin formation was also confirmed.
Article
Medicine, General & Internal
Xiaogang XiA, Ting Li
Summary: Metformin's potential role in inhibiting cancer cell growth has gained attention, but its underlying mechanism is not fully understood. PD-L1 has been identified as a potential strategy against multiple malignancies. However, the association between metformin and PD-L1 in anti-tumor function needs to be elucidated.
ACTA MEDICA MEDITERRANEA
(2023)
Article
Multidisciplinary Sciences
Carlos Rivera, Hun-Goo Lee, Anna Lappala, Danni Wang, Veronica Noches, Montserrat Olivares-Costa, Marcela Sjoberg-Herrera, Jeannie T. Lee, Maria Estela Andres
Summary: RCOR1 is paradoxically enriched in transcriptionally active chromatin and represses transcription through two mechanisms.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Shihao Zhang, Xilan Yu, Yuan Zhang, Xiangyan Xue, Qi Yu, Zitong Zha, Madelaine Gogol, Jerry L. Workman, Shanshan Li
Summary: Telomeres, organized into heterochromatin, require maintenance of silent heterochromatin for chromosome stability. The study shows that Pyruvate kinase Pyk1 phosphorylates histone H3T11 and regulates gene expression through the SESAME complex. SESAME phosphorylates H3T11 at telomeres to maintain SIR complex occupancy and prevent autophagy-mediated Sir2 degradation, ultimately enhancing telomere silencing and protecting against compromised telomere silencing during aging.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Yu -Min Li, Yu -Chao Mei, Ao-Hui Liu, Ru-Xin Wang, Runfa Chen, Hai-Ning Du
Summary: Loss of H3K36me3 during aging is caused by the degradation of Set2 through the actions of Ubc3 and Bre1. Lack of Bre1 or removal of ubiquitination stabilizes Set2, sustains H3K36me3 levels, and extends lifespan.
Article
Developmental Biology
Jessica Ispada, Aldcejam Martins da Fonseca Junior, Otavio Luiz Ramos Santos, Camila Bruna de Lima, Erika Cristina dos Santos, Vinicius Lourenco da Silva, Fernanda Nascimento Almeida, Saul de Castro Leite, Pablo Juan Ross, Marcella Pecora Milazzotto
Summary: This study investigates the relationship between metabolic differences and histone post-translational modifications in bovine embryos, finding distinct patterns of H3K9ac and H3K9me3 between embryos with different development kinetics. The differences in H3K9ac were explained by metabolic pathways influencing acetyl-CoA production.
Article
Cell Biology
Maria Laura Di Giorgio, Patrizia Morciano, Elisabetta Bucciarelli, Antonella Porrazzo, Francesca Cipressa, Sara Saraniero, Diana Manzi, Yikang S. Rong, Giovanni Cenci
Article
Genetics & Heredity
Paolo Maccallini, Francesca Bavasso, Livia Scatolini, Elisabetta Bucciarelli, Gemma Noviello, Veronica Lisi, Valeria Palumbo, Simone D'Angeli, Stefano Cacchione, Giovanni Cenci, Laura Ciapponi, James Wakefield, Maurizio Gatti, Grazia Daniela Raffa
Review
Biochemistry & Molecular Biology
Stefano Cacchione, Giovanni Cenci, Grazia Daniela Raffa
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Article
Biology
Nowsheen Mullani, Yevheniia Porozhan, Adele Mangelinck, Christophe Rachez, Mickael Costallat, Eric Batsche, Michele Goodhardt, Giovanni Cenci, Carl Mann, Christian Muchardt
Summary: The accumulation of senescent cells is a key factor leading to chronic inflammation in aging, driven by both cytoplasmic DNA and double-stranded RNA. In a subset of senescent cells, repeat-containing transcripts stabilized by oxidative stress or reduced RNA exosome activity play a role in perpetuating the inflammatory state characteristic of cellular aging.
LIFE SCIENCE ALLIANCE
(2021)
Article
Clinical Neurology
Antonella Sferra, Paola Fortugno, Marialetizia Motta, Chiara Aiello, Stefania Petrini, Andrea Ciolfi, Francesca Cipressa, Isabella Moroni, Vincenzo Leuzzi, Luisa Pieroni, Federica Marini, Odile Boespflug Tanguy, Eleonore Eymard-Pierre, Federica Rachele Danti, Claudia Compagnucci, Giovanna Zambruno, Alfredo Brusco, Filippo M. Santorelli, Luisa Chiapparini, Paola Francalanci, Anna Livia Loizzo, Marco Tartaglia, Gianluca Cestra, Enrico Bertini
Summary: Leukodystrophies are rare inherited disorders affecting the white matter of the CNS, characterized by glial cell and myelin sheath pathology. Mutations in RNF220 gene were identified as causative for a novel form of leukodystrophy with ataxia and sensorineural deafness. The study also revealed the crucial role of RNF220 in nuclear lamina regulation, highlighting the direct link between nuclear lamina dysfunction and neurodegeneration.
Article
Biochemistry & Molecular Biology
Patrizia Morciano, Maria Laura Di Giorgio, Liliana Tullo, Giovanni Cenci
Summary: In this study, it was found that DmATPCL plays a role in the organization of the acrosome during Drosophila spermatogenesis, suggesting its involvement in the assembly of Golgi-derived structures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Antonella Porrazzo, Giuseppe Esposito, Daniela Grifoni, Giovanni Cenci, Patrizia Morciano, Maria Antonella Tabocchini
Summary: Evidence from studies conducted in deep underground laboratories suggests that environmental radiation may trigger biological mechanisms to cope with genotoxic stress. Our study found that Drosophila neuroblasts are more sensitive to radiation-induced DNA breaks in the reduced radiation background of the LNGS underground laboratory, but this sensitivity can be rescued by increasing the underground gamma dose rate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Genetics & Heredity
Ki Moon Seong, Giovanni Cenci
FRONTIERS IN GENETICS
(2022)
Review
Biochemistry & Molecular Biology
Silvia Pomella, Antonella Porrazzo, Matteo Cassandri, Simona Camero, Silvia Codenotti, Luisa Milazzo, Francesca Vulcano, Giovanni Barillari, Giovanni Cenci, Cinzia Marchese, Alessandro Fanzani, Francesca Megiorni, Rossella Rota, Francesco Marampon
Summary: This review summarizes the classification and management of rhabdomyosarcoma, with a focus on radiotherapy treatment and possible radiosensitizing strategies. It is found that rhabdomyosarcoma has radioresistance, and further studies are needed to dissect the molecular mechanisms of radioresistance in order to improve the efficacy of radiotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
A. Porrazzo, F. Cipressa, A. De Gregorio, C. De Pitta, G. Sales, L. Ciapponi, P. Morciano, G. Esposito, M. A. Tabocchini, G. Cenci
Summary: Chronic low-dose radiation exposure reduces chromosome breaks and telomere dysfunction caused by high-dose irradiation in Drosophila cells. This protective response is associated with the downregulation of the Loquacious D gene, which is involved in esiRNA biogenesis. Loss of Loquacious D mimics the protective effect of low-dose radiation, suggesting its importance in the cellular response to radiation.
COMMUNICATIONS BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Sara Vaccaro, Alessandra Rossetti, Antonella Porrazzo, Simona Camero, Matteo Cassandri, Silvia Pomella, Miriam Tomaciello, Giampiero Macioce, Francesca Pedini, Giovanni Barillari, Cinzia Marchese, Rossella Rota, Giovanni Cenci, Mario Tombolini, Robert A. Newman, Peiying Yang, Silvia Codenotti, Alessandro Fanzani, Francesca Megiorni, Claudio Festuccia, Giuseppe Minniti, Giovanni Luca Gravina, Francesca Vulcano, Luisa Milazzo, Francesco Marampon
Summary: The therapeutic properties of PBI-05204, an extract from Nerium oleander, were studied in the treatment of rhabdomyosarcoma (RMS). PBI-05204 inhibited the growth and invasion abilities of RMS and improved its sensitivity to radiotherapy. Additionally, PBI-05204 suppressed the formation of cancer stem cells in RMS.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Hemakumar M. Reddy, Thomas A. Randall, Francesca Cipressa, Antonella Porrazzo, Giovanni Cenci, Radmila Capkova Frydrychova, James M. Mason
Summary: The length of telomeres in Drosophila melanogaster is maintained through the transposition of retrotransposons Het-A, TART, and TAHRE, unlike most other eukaryotes that rely on telomerase activity. A dominant mutation Tel(1) causing elongation of telomeres was identified in this study and molecularly characterized to be a 3 bp deletion within intron 8 of the Ino80 gene. Deletions induced by CRISPR/Cas9 in the same region exhibited the Tel(1) telomere phenotype, confirming the importance of this intron 8 gene sequence in regulating Drosophila telomere length.
Article
Cell Biology
Simona Rossi, Michela Di Salvio, Marilisa Bali, Assia De Simone, Savina Apolloni, Nadia D'Ambrosi, Ivan Arisi, Francesca Cipressa, Mauro Cozzolino, Gianluca Cestra
Summary: Compelling evidence suggests that defects in nucleocytoplasmic transport are involved in the development of amyotrophic lateral sclerosis (ALS). Hexanucleotide repeat expansions in C9orf72, the most common genetic cause of ALS, have a widespread impact on the transport machinery that controls the distribution of proteins and RNAs. This study demonstrates that C9orf72 toxic species specifically affect the membrane trafficking route regulated by ArfGAP-1.
Article
Cell Biology
Giuseppe Bosso, Francesca Cipressa, Liliana Tullo, Giovanni Cenci
Summary: CBX3 overexpression is common in cancer and promotes cancer cell proliferation, serving as a poor prognosis marker in various types of human cancers. Co-amplification of CBX3 gene with either EGFR or RAC1 results in increased levels of CBX3, EGFR, and RAC1. Simultaneous overexpression of CBX3, RAC1, and EGFR is associated with worse prognosis compared to singular upregulation of these genes. Co-occurrence of low-grade and high-grade amplification between CBX3 and EGFR or RAC1 is linked to reduced patient lifespan. Genetic interactions between CBX3 and RAC1/EGFR are observed in Drosophila melanogaster, suggesting evolutionarily conserved functional relationships.
CELL DEATH DISCOVERY
(2023)