Article
Biochemistry & Molecular Biology
Yukiko Imai, Nobuhito Mori, Yuma Nihashi, Yutaro Kumagai, Yoichiro Shibuya, Junya Oshima, Masahiro Sasaki, Kaoru Sasaki, Yukiko Aihara, Mitsuru Sekido, Yasuyuki S. Kida
Summary: By analyzing gene expression and histological morphology, we found that the patient-derived keloid model reflects scar contraction. Additionally, conditioned media derived from adipose stem cells (ASC-CM) were found to suppress scar contracture by inhibiting inflammation-related gene expression in the early phase and promoting extracellular matrix-related gene expression in the later phase.
Review
Cell & Tissue Engineering
Shengxi Zhou, Mengbo Xie, Jingjing Su, Bingjie Cai, Jingan Li, Kun Zhang
Summary: Scars caused by skin injuries after burns, wounds, abrasions and operations have serious physical and psychological effects on patients. In recent years, the research of scar-free wound repair has been greatly expanded. This paper reviews the types of wounds, the mechanism of scar formation in the healing process, and the current research progress on scar prevention and strategies for scar-free wound repair.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Huimin Zhang, Chengyu Zang, Wen Zhao, Linfeng Zhang, Rui Liu, Zhang Feng, Jie Wu, Rongtao Cui
Summary: The study reveals that TNFSF13 activates the NF-κB signaling pathway by interacting with HSPG2, regulating the proliferation, migration, fibrosis and inflammatory response of HSF. MSC-exo inhibits the proliferation and migration of HSF by suppressing TNFSF13 and HSPG2.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Hui Song Cui, So Young Joo, Seung Yeol Lee, Yoon Soo Cho, Dong Hyun Kim, Cheong Hoon Seo
Summary: It is unclear how scar fibroblasts (SFs) affect keratinocytes in hypertrophic scars (HTS) through cell-cell interaction. This study investigated the effects of HTS-derived exosomes on the proliferation and differentiation of normal human keratinocytes (NHKs) and compared them with normal fibroblasts (NFs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Emergency Medicine
Jie Li, Yan Li, Yunchuan Wang, Xiang He, Jing Wang, Weixia Cai, Yanhui Jia, Dan Xiao, Jian Zhang, Ming Zhao, Kuo Shen, Zichao Li, Wenbin Jia, Kejia Wang, Yue Zhang, Linlin Su, Huayu Zhu, Dahai Hu
Summary: MicroRNA-101 (miR-101) is a tumor suppressor microRNA associated with various diseases, but its role in hypertrophic scars (HS) remained unclear. Research showed that low miR-101 expression in HS and HSF, overexpressing miR-101 reduced collagen expression in HSF, while high EZH2 expression was detected in HS and HSF. MiR-101 targeted EZH2 to suppress collagen deposition in HS.
Article
Multidisciplinary Sciences
Jun Jie Tan, Jacques P. Guyette, Kenji Miki, Ling Xiao, Gurbani Kaur, Tong Wu, Liye Zhu, Katrina J. Hansen, King-Hwa Ling, David J. Milan, Harald C. Ott
Summary: The study demonstrates the generation of premature PECs using BVR stimulation, co-culturing PECs with cardiomyocytes leads to cardiomyocyte aggregation, proliferation and the formation of more mature structures with improved beating/contractility.
NATURE COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Fei Wu, Yi Gong, Lili Song, Haoyi Li, Xiangmin Zhang, Hongyan Li, Shicui Zhang
Summary: This study demonstrates that Pt5-1c promotes wound closure in vitro and accelerates healing and re-epithelialization in vivo. It activates migration and proliferation of fibroblasts and promotes collagen contraction. Pt5-1c shows potential as a therapeutic candidate for promoting wound healing.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Integrative & Complementary Medicine
Zha Ru, Ying Hu, Shenghua Huang, Li Bai, Kun Zhang, Yue Li
Summary: Galangin can alleviate hypertrophic scars by regulating the TGF-beta/Smad signaling pathway, leading to a reduction in collagen deposition.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Engineering, Biomedical
Yueming Xue, Chunxiao Qi, Yunsheng Dong, Lin Zhang, Xiangsheng Liu, Yufei Liu, Shufang Wang
Summary: The new hydrogel developed in this project shows potential in inhibiting overgrowth of scars and promoting cell adhesion, providing a new treatment option for treating serious skin disorders.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2021)
Article
Cell Biology
Shiyi Li, Jinxiu Yang, Jiachen Sun, Minliang Chen
Summary: This study demonstrates that human adipose-derived mesenchymal stem cells (AD-MSCs) can effectively cure hypertrophic scars by promoting the apoptosis of hypertrophic scar fibroblasts (HSFs) and inhibiting their proliferation and migration. The therapeutic effect is associated with the inhibition of nuclear factor erythroid-2-related factor 2 (Nrf2) expression in HSFs. AD-MSCs may provide an alternative approach for treating hypertrophic scars.
Article
Cell & Tissue Engineering
Qun Qian, Ni Zhu, Wenzhe Li, Songlin Wan, Dongcheng Wu, Yunhua Wu, Weicheng Liu
Summary: The study found that human umbilical mesenchymal stem cells (HUMSCs)-derived microvesicles (HUMSCs-MVs) can inhibit the formation of hypertrophic scars by suppressing fibroblast proliferation and invasion, inflammation and oxidative stress, Smad2/3 activation, and angiogenesis. This suggests that HUMSCs-MVs may be a potential drug for attenuating hypertrophic scar formation during wound healing.
STEM CELLS INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Behnaz Bakhshandeh, Zohreh Jahanafrooz, Shiva Allahdadi, Shiva Daryani, Zahra Dehghani, Mahya Sadeghi, Mir Sepehr Pedram, Mohammad Mehdi Dehghan
Summary: Induced pluripotent stem cells (iPSCs) can secrete microvesicles (MVs) that have the potential for skin healing and rejuvenation. This study evaluated the possibility, safety, and effectiveness of using iPSCs-derived MVs for skin tissue engineering and regeneration. The results showed that MVs can promote skin cell growth and repair, and have beneficial effects on skin regeneration in rat burn wound models.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Guojun Hu, Yafeng Dong, Zunping Zhang, Xudong Fan, Fang Ren
Summary: This study found that it was difficult for ribavirin to eliminate apple scar skin viroid from apple plants.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biochemistry & Molecular Biology
Hui Song Cui, Dong Hyun Kim, So Young Joo, Yoon Soo Cho, June-Bum Kim, Cheong Hoon Seo
Summary: In this study, it was found that exosomes derived from hypertrophic scar fibroblasts can change fibrosis-related signaling pathways in normal fibroblasts, leading to increased cell proliferation, migration, and epithelial-mesenchymal transition, as well as increased expression of fibronectin, type I collagen, and type III collagen.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Agriculture, Dairy & Animal Science
Artur Bryja, Grzegorz Latosinski, Maurycy Jankowski, Ana Angelova Volponi, Paul Mozdziak, Jamil A. Shibli, Rut Bryl, Julia Spaczynska, Hanna Piotrowska-Kempisty, Krzysztof Krawiec, Bartosz Kempisty, Marta Dyszkiewicz-Konwinska
Summary: Domestic pigs have high phylogenetic similarity to humans and are used in biomedical research. Porcine tissues are used in human skin transplants and tissue reconstruction. Transcriptional analysis and live-cell imaging are powerful tools to study complex biological systems, such as porcine oral mucosal cells. Transcriptional analysis revealed gene expression changes over time in in vitro culture, while computer analysis of images allowed identification of different cell populations.
Article
Critical Care Medicine
Molly E. Baumann, Danielle M. DeBruler, Britani N. Blackstone, Rebecca A. Coffey, Steven T. Boyce, Dorothy M. Supp, J. Kevin Bailey, Heather M. Powell
Summary: Assessing scar properties quantitatively with non-invasive instruments is more reliable and efficient than using scar scales, with color and biomechanics measurements taking less than 90 s per measurement and providing high sensitivity over time or treatment.
Letter
Critical Care Medicine
Heather M. Powell, Dorothy M. Supp, J. Kevin Bailey, Molly E. Baumann
Article
Critical Care Medicine
Angela L. F. Gibson, Bonnie C. Carney, Leila Cuttle, Christine J. Andrews, Christine J. Kowalczewski, Aiping Liu, Heather M. Powell, Randolph Stone, Dorothy M. Supp, Adam J. Singer, Jeffrey W. Shupp, Lily Stalter, Lauren T. Moffatt
Summary: Deep partial thickness burns are commonly seen in clinical practice but are hard to diagnose. Accurate animal models are crucial for developing methods to assess burn depth and therapies to treat these burns. Inconsistencies in evaluation approaches among different laboratories were found, leading to the development of a scoring rubric that improved reliability in assessing burn injury depth in pig models.
JOURNAL OF BURN CARE & RESEARCH
(2021)
Article
Cell & Tissue Engineering
Sonya Ruiz-Torres, Marion G. Brusadelli, David P. Witte, Kathryn A. Wikenheiser-Brokamp, Sharon Sauter, Adam S. Nelson, Mathieu Sertorio, Timothy M. Chlon, Adam Lane, Parinda A. Mehta, Kasiani C. Myers, Mary C. Bedard, Bidisha Pal, Dorothy M. Supp, Paul F. Lambert, Kakajan Komurov, Melinda Butsch Kovacic, Stella M. Davies, Susanne Wells
Summary: The study found that genetic mutations in Fanconi anemia (FA) patients can affect cell structure and function, accelerating the process of cancer development. This provides a new direction for exploring SCC prevention.
Article
Cell Biology
Jennifer M. Hahn, Kevin L. McFarland, Kelly A. Combs, Meridith C. Anness, Dorothy M. Supp
Summary: Keloids are fibroproliferative lesions resulting from abnormal wound healing, with different expression of HOX genes in keloid and normal skin cells potentially playing a role in keloid development. However, the differential expression of HOX genes may be due to positional identity differences between keloid and normal fibroblasts rather than body site-specific factors.
WOUND REPAIR AND REGENERATION
(2021)
Article
Multidisciplinary Sciences
Christian B. Bergmann, Bruce D. Hammock, Debin Wan, Falk Gogolla, Holly Goetzman, Charles C. Caldwell, Dorothy M. Supp
Summary: The study demonstrated that TPPU administration can decrease DHET levels post-burn, leading to reduced IL-6 concentrations. 14,15-DHET was found to modulate neutrophil functionality and mRNA expression, indicating its role in regulating inflammation in burn injury.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Alexander Bondoc, Kathryn Glaser, Kang Jin, Charissa Lake, Stefano Cairo, James Geller, Gregory Tiao, Bruce Aronow
Summary: This study utilized single cell RNA sequencing to analyze gene expression patterns in hepatoblastoma tumor, identifying a driver malignant cell cluster and proposing potential roles in treatment research.
COMMUNICATIONS BIOLOGY
(2021)
Article
Critical Care Medicine
David M. Burmeister, Dorothy M. Supp, Richard A. Clark, Edward E. Tredget, Heather M. Powell, Perenlei Enkhbaatar, Julia K. Bohannon, Leopoldo C. Cancio, David M. Hill, Rachel M. Nygaard
Summary: This article introduces the use of multiple animal species and approaches for modeling burn care and discusses the pros and cons of using small and large animal models for research. Researchers believe that using pigs as large animal models can better simulate human skin, while sheep models are suitable for studying inhalation injury. However, these models are expensive and technically demanding. Therefore, by improving small animal models, questions related to burns can be addressed.
JOURNAL OF BURN CARE & RESEARCH
(2022)
Review
Oncology
Emily J. Schepers, Kathryn Glaser, Harrison M. Zwolshen, Stephen J. Hartman, Alexander J. Bondoc
Summary: This article reviews the structure and function of GPC3 in liver cancer, specifically focusing on the posttranslational modifications (PTM) that regulate its tertiary and quaternary structures. The study proposes that GPC3's function in normal development can be influenced by extensive PTMs, and dysregulation of these processes can lead to disease. Understanding the regulatory impact of these modifications provides insights into GPC3's role in oncogenesis, epithelial-mesenchymal transition, and drug development.
Article
Cell Biology
Jennifer M. Hahn, Kelly A. Combs, Heather M. Powell, Dorothy M. Supp
Summary: Keloids are challenging to treat and have high recurrence rates. The genetic causes and molecular mechanisms of keloid formation are not fully understood. Dark-skinned individuals, who are at higher risk of keloids, may also have lower vitamin D levels, suggesting a potential role for vitamin D in keloid pathology. Studies have found reduced vitamin D receptor expression in keloid lesions, indicating a possible therapeutic role for vitamin D in suppressing keloid fibrosis.
WOUND REPAIR AND REGENERATION
(2023)
Article
Oncology
Kathryn Glaser, Emily J. Schepers, Harrison M. Zwolshen, Charissa M. Lake, Nikolai A. Timchenko, Rebekah A. Karns, Stefano Cairo, James I. Geller, Gregory M. Tiao, Alexander J. Bondoc
Summary: This study reveals that EZH2 promotes proliferation in HB tumor-derived cell lines through interaction with beta-catenin, suggesting a noncanonical mechanism contributing to HB pathogenesis. Further mechanistic studies are needed to explore potential downstream mechanisms and translational potential of EZH2 inhibitors for HB treatment.
PEDIATRIC BLOOD & CANCER
(2023)
Article
Biochemical Research Methods
Dorothy M. Supp, Jennifer M. Hahn, Kelly A. Combs, Kevin L. Mcfarland, Heather M. Powell
Summary: A method has been developed to isolate four types of primary cells from a single human skin sample without using murine feeder cells. These isolated cells contain stem cell populations and are capable of forming functional skin tissue.
Meeting Abstract
Cell Biology
Dorothy M. Supp, Jennifer M. Hahn, Kevin L. McFarland, Kelly A. Combs, Meridith Anness
WOUND REPAIR AND REGENERATION
(2021)
Meeting Abstract
Critical Care Medicine
Christian B. Bergmann, Bruce D. Hammock, Debin Wan, Dorothy M. Supp, Charles Caldwell
Article
Emergency Medicine
Latha Satish, Alexander Evdokiou, Eleni Geletu, Jennifer M. Hahn, Dorothy M. Supp