Article
Engineering, Biomedical
Chenchen Zhou, Chunli Wang, Kang Xu, Zhixing Niu, Shujuan Zou, Demao Zhang, Zhiyong Qian, Jinfeng Liao, Jing Xie
Summary: Cartilage injury is a common problem with limited repair options. This study focuses on a hydrogel scaffold made from methacrylated gelatin (GelMA) and iron oxide nanoparticles (Fe2O3), and investigates its mechanical properties as well as its interaction with chondrocytes. The results show that the stiffness of the Fe2O3/GelMA hydrogel can be controlled by adjusting the concentration of magnetic nanoparticles, and that this hybrid hydrogel promotes cell morphology changes and metabolic activities in chondrocytes. The study also demonstrates the potential of the Fe2O3/GelMA hydrogel for tissue engineering applications.
BIOACTIVE MATERIALS
(2023)
Article
Orthopedics
Y. He, M. Zhang, J. Song, M. L. Warman
Summary: The study showed that cell-depleted areas within MCC cartilage are not repopulated by new cells in adult mice after diphtheria toxin-mediated chondrocyte killing.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Engineering, Biomedical
Si Hwa Choi, Kijun Lee, Heeju Han, Hyunkyung Mo, Hyerin Jung, YoungWoo Ryu, Yoojun Nam, Yeri Alice Rim, Ji Hyeon Ju
Summary: Cartilage is a tissue with limited regeneration ability, and stem cell-based therapies have shown promise in cartilage regeneration. This study explored the potential of stem cell-derived chondrocyte ECM for cartilage regeneration. The decellularized ECM from cultured chondrocytes enhanced chondrogenesis and restored cartilage defects in a rat osteoarthritis model. The findings suggest that hiPSC-derived cartilage-like dECM could serve as a noncellular therapeutic for articu-lar cartilage reconstruction.
ACTA BIOMATERIALIA
(2023)
Article
Cell Biology
Carlos Martinez-Armenta, Carlos Suarez-Ahedo, Anell Olivos-Meza, Maria C. Camacho-Rea, Laura E. Martinez-Gomez, Guadalupe Elizabeth Jimenez-Gutierrez, Gabriela A. Martinez-Nava, Luis E. Gomez-Quiroz, Carlos Pineda, Alberto Lopez-Reyes
Summary: In this study, a scaffold-free 3D culture system was developed to generate spheroids from human chondrocytes. The results showed that spheroids developed in a hypoxic environment exhibited better chondrogenesis characteristics compared to those developed under normoxic conditions. This novel culture system could be a valuable tool for cartilage biology research.
Review
Chemistry, Medicinal
Jhi Biau Foo, Qi Hao Looi, Chee Wun How, Sau Har Lee, Maimonah Eissa Al-Masawa, Pei Pei Chong, Jia Xian Law
Summary: Exosomes are small extracellular vesicles secreted by cells for intercellular communication, with miRNAs being a promising therapeutic factor for cartilage regeneration. They can promote chondrocyte proliferation and matrix secretion, reduce scar tissue formation, and decrease inflammation, but the optimization of exosome therapy for cartilage injuries is still lacking.
Article
Biochemistry & Molecular Biology
Anthony J. Hayes, John Whitelock, James Melrose
Summary: The aim of this study was to investigate the roles of perlecan in the regulation of cartilage development and homeostasis. The study showed that perlecan interacts with various molecules to promote matrix stabilization, cellular proliferation, and tissue expansion. Furthermore, perlecan is involved in chromatin structure modulation and gene regulation. Snail-1 and FGF family members play important roles in chondrogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Biomaterials
Cynthia M. Co, Samira Izuagbe, Jun Zhou, Ning Zhou, Xiankai Sun, Joseph Borrelli, Liping Tang
Summary: A novel cell-based therapy has been developed for treating articular cartilage injuries, involving the pre-targeting of apoptotic chondrocytes and the delivery of healthy, metabolically active chondrocytes using click chemistry. The effectiveness of this approach was demonstrated in reducing cartilage degeneration and enhancing ECM regeneration in an ex vivo human cartilage explant PTOA model. This Tz-TCO click chemistry-mediated cell delivery approach shows great potential in clinical applications for targeting and treating cartilage injury.
REGENERATIVE BIOMATERIALS
(2021)
Article
Geriatrics & Gerontology
Quanzhi Liang, Ailijiang Asila, Yingjie Deng, Jun Liao, Zhenfeng Liu, Rui Fang
Summary: Osteopontin-induced HOTAIR expression is involved in osteoarthritis by regulating cell proliferation. The study found that HOTAIR was upregulated in OA and showed a positive correlation with osteopontin. While osteopontin treatment increased HOTAIR expression in primary chondrocytes, HOTAIR overexpression and knockdown did not significantly affect osteopontin expression. Additionally, both osteopontin and HOTAIR overexpression increased chondrocyte proliferation, while HOTAIR knockdown decreased chondrocyte proliferation and reduced the effects of osteopontin treatment on cell proliferation.
Article
Geriatrics & Gerontology
Xiaoxin Wu, Xiwei Fan, Ross Crawford, Yin Xiao, Indira Prasadam
Summary: The function of articular cartilage relies on the coordinated metabolic regulation in chondrocytes. Dysregulation of cellular metabolism can lead to degenerative diseases like osteoarthritis. Chondrocytes in cartilage have limited access to nutrients and oxygen, as well as pathways for metabolite removal. Central carbon metabolism pathways are crucial for maintaining cartilage homeostasis. Increased understanding of cellular metabolism and its dysregulation in osteoarthritis can provide insights into potential therapeutic opportunities.
Article
Chemistry, Multidisciplinary
Elizabeth R. Kahle, Biao Han, Prashant Chandrasekaran, Evan R. Phillips, Mary K. Mulcahey, X. Lucas Lu, Michele S. Marcolongo, Lin Han
Summary: Molecular engineering using synthetic mimics of native matrix molecules can modulate the mechanical properties of cellular microenvironment and influence cell mechanobiology. This study demonstrated the use of biomimetic proteoglycans (BPGs) to engineer the micromechanics of the pericellular matrix (PCM) in cartilage, leading to enhanced cellular mechanotransduction. The interactions between BPGs and the native PCM were facilitated by the biomimetic ultrastructure of BPGs, and showed potential for improving tissue regeneration and disease modification in various cell types.
Article
Biotechnology & Applied Microbiology
Yu Seon Kim, Shail M. Mehta, Jason L. Guo, Hannah A. Pearce, Brandon T. Smith, Emma Watson, Gerry L. Koons, Adam M. Navara, Johnny Lam, K. Jane Grande-Allen, Antonios G. Mikos
Summary: This study investigated the chondrogenic activity of encapsulated MSCs and ACs in hydrogels, as well as the impact of PLL on their mechanical properties. The highest chondrogenic activity was observed in AC-encapsulated hydrogels, while no significant differences were found in mechanical properties among the study groups. The results suggest that the ex vivo model can provide insights into the relationship between biochemical changes and mechanical properties in hydrogels.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Cell Biology
Pranidhi Baddam, Daniel Young, Garett Dunsmore, Chunpeng Nie, Farah Eaton, Shokrollah Elahi, Juan Jovel, Adetola B. Adesida, Antoine Dufour, Daniel Graf
Summary: This study uncovers the cellular and molecular abnormalities underlying NSD using gene expression, proteomics, and immunofluorescence analysis. The investigation provides a genetic example of an in vivo cartilage fate switch controlled by BMP2 and BMP7 balance. Similar cellular and molecular changes in NSD and knee OA suggest a related etiology underlying these cartilage abnormalities.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Hongyuan Zhang, Vijitha Puviindran, Puviindran Nadesan, Xiruo Ding, Leyao Shen, Yuning J. Tang, Hidetoshi Tsushima, Yasuhito Yahara, Ga Ban, Guo-Fang Zhang, Courtney M. Karner, Benjamin A. Alman
Summary: This study demonstrates the distinct roles of glutamine metabolism in enchondromas and chondrosarcomas with IDH1 or IDH2 mutations. Glutamine promotes cell differentiation and proliferation in enchondromas, while inhibiting glutaminase reduces overall tumor burden in chondrosarcomas. These findings suggest potential therapeutic approaches to suppress the growth of enchondroma and chondrosarcoma tumors through modulation of glutamine metabolism.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Sang-Soo Han, Myung-Ok Cho, Kang Moo Huh, Sun-Woong Kang
Summary: Nanoscale structures can directly control cellular behaviors and reduce proliferation rate. Using nanopatterned dishes can lower COL10 mRNA levels in chondrocytes and induce cell cycle arrest.
Article
Cell Biology
Junjie Wang, Zewen Sun, Chenghao Yu, Haibo Zhao, Mingyue Yan, Shenjie Sun, Xu Han, Tingting Jiang, Tianrui Wang, Tengbo Yu, Yingze Zhang
Summary: This study used single-cell RNA sequencing to analyze chondrocytes under different force conditions. Nine chondrocyte subsets were identified, and differences between the AT and ATa groups were analyzed. The results showed that force activated reactive oxygen species related processes and tissue repair processes were common in the force-bearing region. Prehypertrophic chondrocytes seemed to play an important role in the ankle.
CELL BIOLOGY AND TOXICOLOGY
(2023)
