Article
Chemistry, Medicinal
Sandesh Deshpande, Wenjuan Huo, Rinu Shrestha, Kevin Sparrow, James M. Wood, Gary B. Evans, Lawrence D. Harris, Richard L. Kingston, Esther M. M. Bulloch
Summary: Galidesivir, an adenosine nucleoside analog, has been shown to inhibit the replication of dengue and Zika viruses in cell-based assays and animal models. In this study, the researchers directly tested the effects of Galidesivir on the polymerase activity of dengue-2 and Zika viruses. The results suggest that Galidesivir's inhibition of viral replication may not be due to the early termination of RNA synthesis.
ACS INFECTIOUS DISEASES
(2023)
Article
Cell Biology
Nicolas Fossat, Emma A. Lundsgaard, Rui Costa, Lizandro R. Rivera-Rangel, Louise Nielsen, Lotte S. Mikkelsen, Santseharay Ramirez, Jens Bukh, Troels K. H. Scheel
Summary: We used CLEAR-CLIP to investigate the interaction between SARS-CoV-2 and miRNA, revealing the regulation of cellular miRNA and the interaction with miR-15 family during infection. We found that these interactions are not critical for virus production. This work contributes to understanding the interplay between miRNA regulation and SARS-CoV-2 infection.
Article
Immunology
Irma F. Wahongan, Elly J. Suoth, Fatimawali, Saad Alhumaid, Hawra Albayat, Mohammed Aljeldah, Basim R. Al Shammari, Mutaib M. Mashraqi, Ahmad A. Alshehri, Tarek Sulaiman, Safaa A. Turkistani, Ameen S. S. Alwashmi, Mohammed Garout, Trina Ekawati Tallei, Ali A. Rabaan
Summary: Dengue fever remains a health concern in tropical and subtropical regions with no definitive vaccine. An epitope-based vaccine derived from DENV-2 RdRp has shown potential to induce an immunological response, binding stably to dendritic cell receptors and possibly serving as a universal dengue virus vaccine candidate.
Article
Biochemistry & Molecular Biology
Priscilla L. S. Boon, Ana S. Martins, Xin Ni Lim, Francisco J. Enguita, Nuno C. Santos, Peter J. Bond, Yue Wan, Ivo C. Martins, Roland G. Huber
Summary: Dengue virus is a significant health risk in tropical and subtropical regions. The structure of the packaged genome in complex with C proteins remains elusive. This study investigates the interactions of C proteins with viral RNA and shows their role in coordinating key interactions for proper packaging of viral RNA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Vivek Dhar Dwivedi, Aditya Arya, Pardeep Yadav, Rajesh Kumar, Vinod Kumar, Gajendra P. S. Raghava
Summary: The DenvinD database provides comprehensive information on experimentally validated DENV inhibitors against known targets, and offers user-friendly web-based features for simple search, advanced search, and data browsing. This database is the first useful repository of its kind, aiding in advancing DENV drug discovery research.
BRIEFINGS IN BIOINFORMATICS
(2021)
Review
Immunology
Shenghai Shen, Li-Sheng Zhang
Summary: Post-transcriptional RNA modifications play a crucial role in the dynamic regulation of gene expression in various biological processes. This review summarizes the functional roles of non-m(6)A RNA modifications in antiviral innate immunity, provides a systematic introduction to their biogenesis and functions in viral and host RNA, and discusses the recent progress in developing antiviral drugs targeting these modifications.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Virology
Thamir A. Alandijany, Mai M. El-Daly, Ahmed M. Tolah, Leena H. Bajrai, Aiah M. Khateb, Isra M. Alsaady, Sarah A. Altwaim, Amit Dubey, Vivek Dhar Dwivedi, Esam I. Azhar
Summary: The World Health Organization (WHO) has identified the Zika virus (ZIKV) as a significant risk to public health. Current treatments and vaccines are not available, so it is crucial to develop an efficient therapeutic molecule. A computational search was conducted to identify compounds that can bind and block the activity of ZIKV NS5 RNA-dependent RNA polymerase (RdRp). Compound 127042987 showed the highest binding affinity and stable interaction with the protein, making it a potential therapeutic option to prevent Zika virus infection.
Review
Biochemistry & Molecular Biology
Katherine E. Bohnsack, Nicole Kleiber, Nicolas Lemus-Diaz, Markus T. Bohnsack
Summary: Modified nucleotides within cellular RNAs play significant roles in their biogenesis, stability, and function. The recent discovery of the methyltransferases responsible for installing 3-methylcytidine (m(3)C(32)) in human tRNAs has shed light on the importance of this modification. Mechanistic details of how these methyltransferases recognize their substrate tRNAs have been uncovered, along with the biological and functional relevance of the interconnections between m(3)C(32) and modified adenosines at position 37. Functional insights suggest that m(3)C(32) modifications influence tRNA structure and impaired translation is observed in the absence of these modifications. The development of quantitative, transcriptome-wide m(3)C mapping approaches and the discovery of an m(3)C demethylase indicate the dynamic nature of m(3)C, potentially contributing to fine-tuning gene expression in different conditions.
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xin Ee Yong, V. Raghuvamsi Palur, Ganesh S. Anand, Thorsten Wohland, Kamal K. Sharma
Summary: Through its chaperone activity, DENV2C promotes nucleic acid structural rearrangements in the capsid protein of dengue virus strain 2. While not directly impacting the cHP's structural functionality, DENV2C accelerates annealing and strand displacement of RNA elements, providing mechanistic insights into how it regulates RNA synthesis.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Artem Babaian, Robert Edgar
Summary: Researchers have described a new method for characterizing RNA viruses by identifying palmprints in their polymerase sequences. They have developed an algorithm and a database to support this approach, which paves the way for rapid discovery of RNA viruses in the future.
Article
Biochemistry & Molecular Biology
Sheng Lin, Hua Chen, Zimin Chen, Fanli Yang, Fei Ye, Yue Zheng, Jing Yang, Xi Lin, Honglu Sun, Lingling Wang, Ao Wen, Haohao Dong, Qingjie Xiao, Dong Deng, Yu Cao, Guangwen Lu
Summary: The emergence of SARS-CoV-2 infection poses an unprecedented threat to global public health, with the virus-encoded nsp14 protein playing a critical role in viral replication. Research demonstrates that the SARS-CoV-2 nsp10/nsp14-ExoN complex, along with the binding of the nsp10 co-factor, maintains both structural and functional integrity.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Microbiology
Mayra Diosa-Toro, Debbie R. Kennedy, Vanessa Chuo, Vsevolod L. Popov, Julien Pompon, Mariano A. Garcia-Blanco
Summary: Infection with dengue virus induces rearrangements of the endoplasmic reticulum, and a host RNA-binding protein YBX1 plays a crucial role in viral replication and assembly by interacting with the viral nucleocapsid and participating in assembly sites.
Article
Microbiology
Mayra Diosa-Toro, Debbie R. Kennedy, Vanessa Chuo, Vsevolod L. Popov, Julien Pompon, Mariano A. Garcia-Blanco
Summary: Y-box binding protein 1 (YBX1) interacts with the viral nucleocapsid, distributes to DENV assembly sites, and is necessary for efficient assembly of intracellular infectious virions.
Article
Multidisciplinary Sciences
Inna Ricardo-Lax, Joseph M. Luna, Tran Thi Nhu Thao, Jeremie Le Pen, Yingpu Yu, H-Heinrich Hoffmann, William M. Schneider, Brandon S. Razooky, Javier Fernandez-Martinez, Fabian Schmidt, Yiska Weisblum, Bettina Salome Trueb, Ines Berenguer Veiga, Kimberly Schmied, Nadine Ebert, Eleftherios Michailidis, Avery Peace, Francisco J. Sanchez-Rivera, Scott W. Lowe, Michael P. Rout, Theodora Hatziioannou, Paul D. Bieniasz, John T. Poirier, Margaret R. MacDonald, Volker Thiel, Charles M. Rice
Summary: Molecular virology tools are crucial for studying SARS-CoV-2 and developing therapeutics. A yeast-based reverse genetics system was used to create spike-deleted self-replicating RNAs for SARS-CoV-2. These noninfectious replicons can be complemented with viral glycoproteins for single-cycle delivery into different cell types, making it a versatile platform for antiviral drug screening and viral variant characterization.
Article
Biochemistry & Molecular Biology
Mahtab Peyambari, Shengxi Guan, Marilyn J. Roossinck
Summary: Experimental findings show that RdRp proteins of partitiviruses have reverse transcriptase (RT) function, can be active at lower temperatures, sparking researchers to question why these viruses have RT capabilities.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Immunology
Chun-Kuang Lin, Chin-Kai Tseng, Yu-Hsuan Wu, Chun-Yu Lin, Chung-Hao Huang, Weng-Hung Wang, Chih-Chuang Liaw, Yen-Hsu Chen, Jin-Ching Lee
JOURNAL OF INFECTIOUS DISEASES
(2019)
Article
Medicine, General & Internal
Batbold Batsaikhan, Gantsetseg Gantumur, Ching-I Huang, Ming-Lun Yeh, Chung-Feng Huang, Zu-Yau Lin, Shinn-Cherng Chen, Jee-Fu Huang, Ming-Lung Yu, Wan-Long Chuang, Jin-Ching Lee, Chia-Yen Dai
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2019)
Article
Medicine, General & Internal
Batbold Batsaikhan, Ming-Ying Lu, Ming-Lun Yeh, Ching- Huang, Chung-Feng Huang, Zu-Yau Lin, Shinn-Cherng Chen, Jee-Fu Huang, Ping-Hsin Hsieh, Wan-Long Chuang, Jin-Ching Lee, Ming-Lung Yu, Chia-Yen Dai
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2019)
Article
Gastroenterology & Hepatology
Wei-Chun Chen, Chih-Ku Wei, Jin-Ching Lee
JOURNAL OF VIRAL HEPATITIS
(2019)
Article
Biochemistry & Molecular Biology
Mei-Yueh Lee, Wei-Chun Chen, Wei-Hao Hsu, Szu-Chia Chen, Jin-Ching Lee
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Chemistry, Medicinal
Antonio Coluccia, Michela Puxeddu, Marianna Nalli, Chih-Ku Wei, Yu-Hsuan Wu, Eloise Mastrangelo, Tasneem Elamin, Delia Tarantino, Joachim Jakob Bugert, Benno Schreiner, Juliane Nolte, Frank Schwarze, Giuseppe La Regina, Jin-Ching Lee, Romano Silvestri
ACS MEDICINAL CHEMISTRY LETTERS
(2020)
Article
Biochemistry & Molecular Biology
Yu-Chieh Su, Yi-Fang Huang, Yu-Wen Wu, Hui-Feng Chen, Yu-Hsuan Wu, Chia-Chun Hsu, Yao-Chin Hsu, Jin-Ching Lee
Article
Biochemistry & Molecular Biology
Yu-Tse Kao, Yi-Siao Chen, Kai-Wei Tang, Jin-Ching Lee, Chih-Hua Tseng, Cherng-Chyi Tzeng, Chia-Hung Yen, Yeh-Long Chen
Article
Multidisciplinary Sciences
Yu-Ting Kuo, Ching-Hsuan Liu, Jin-Wei Li, Chien-Ju Lin, Alagie Jassey, Huey-Nan Wu, Guey Chuen Perng, Ming-Hong Yen, Liang-Tzung Lin
SCIENTIFIC REPORTS
(2020)
Article
Virology
Wei-Chun Chen, Chih-Ku Wei, Monir Hossen, Yao-Chin Hsu, Jin-Ching Lee
Summary: This study demonstrated the potential of (E)-guggulsterone against DENV infection by stimulating Nrf2-mediated HO-1 expression, increasing antiviral interferon responses, and blocking DENV NS2B/3B protease activity. In vivo experiments showed that (E)-guggulsterone protected ICR suckling mice from life-threatening DENV infection with dose-dependent inhibitory effects on DENV protein synthesis and RNA replication without cytotoxicity. These findings suggest that (E)-guggulsterone may be a promising supplement for controlling DENV replication.
Article
Pharmacology & Pharmacy
Wei -Chun Chen, Chung-Hao Huang, Wangta Liu, Jin-Ching Lee
Summary: Dengue virus (DENV) infection is a life-threatening disease with no effective treatment options. However, a study found that sulforaphane (SFN) has significant antiviral activity against DENV by inducing heme oxygenase-1 (HO-1) expression and inhibiting viral protease activity. This suggests that SFN could be a promising candidate for treating DENV.
ANTIVIRAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Shih-Han Wang, Yi-Siao Chen, Kuei-Hung Lai, Chung-Kuang Lu, Hsun-Shuo Chang, Ho-Cheng Wu, Feng-Lin Yen, Lo-Yun Chen, Jin-Ching Lee, Chia-Hung Yen
Summary: The extract of Prinsepiae Nux was identified as a potent NRF2 activator and showed potential in preventing UVB-induced skin damage.
Article
Chemistry, Medicinal
I-Wen Lo, Geng-You Liao, Jin-Ching Lee, Chi- Chang, Yang-Chang Wu, Yen-Yu Chen, Shang-Pin Liu, Huey-Jen Su, Chih- Liu, Chia-Yi Kuo, Zheng-Yu Lin, Tsung-Lin Li, Yun-Sheng Lin, Chia-Ching Liaw
Summary: Hybrid natural products produced through mixed biosynthetic pathways have complex structures and diverse medicinal properties. This study reports the discovery of four structurally attractive diterpene-alkaloid conjugates from the barks of Taiwanese Polyalthia longifolia, which exhibit promising anti-DENV2 activity. In vitro experiments and molecular docking analysis suggest that these compounds are novel and effective DENV NS2B-NS3 protease inhibitors.
Article
Virology
Wei-Chun Chen, Monir Hossen, Wangta Liu, Chia-Hung Yen, Chung-Hao Huang, Yao-Chin Hsu, Jin-Ching Lee
Summary: Grape seed proanthocyanidins extract (GSPE) suppresses the replication of DENV and reduces the inflammatory response caused by DENV infection, indicating its potential as a dietary supplement to alleviate DENV infection and severe dengue.
