Article
Chemistry, Multidisciplinary
Lucien Eisenburger, Philipp Strobel, Peter J. Schmidt, Thomas Brauniger, Jonathan Wright, Eleanor Lawrence Bright, Carlotta Giacobbe, Oliver Oeckler, Wolfgang Schnick
Summary: This study synthesized the first nitridic analogs of micas, featuring a rare motif of sixfold coordinated silicon atoms and the presence of H in SiN4(NH)(2) octahedra. Eu2+-doped samples exhibited tunable narrow-band emission.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
David Akopian, Colleen A. McGourty, Michael Rape
Summary: Cuilin-RING E3 ligases (CRLs) are essential ubiquitylation enzymes and the formation of CRL complexes is a tightly regulated process, with co-adaptors playing an important role in CRL assembly.
Editorial Material
Cell Biology
Qi Zhang, Yihong Ye
Summary: In yeast, the vacuole-associated Rsp5 ubiquitin ligase utilizes a substrate adaptor Ssh4 to recognize membrane helices in Ypq1, targeting the lysine transporter for lysosomal degradation during lysine starvation.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Chemistry, Physical
Lucas W. Henderson, Edie M. Sharon, Amit K. S. Gautam, Adam J. Anthony, Martin F. Jarrold, David H. Russell, Andreas Matouschek, David E. Clemmer
Summary: Mass spectrometry studies show that the stability of S. cerevisiae 20S proteasome undergoes related configurations and transitions during temperature changes, possibly linked to the opening of the proteolytic core. The study indicates that the proteasome remains intact and all transitions are reversible. Three types of structures are identified based on thermodynamic analysis: energetically stabilized closed configurations, high-entropy precursor states, and open pore structures. Opening of the 20S pore in the absence of the regulatory unit involves a charge-priming process. However, only a small fraction of precursor configurations actually open to expose the catalytic cavity.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Editorial Material
Cell Biology
Jun-Ichi Sakamaki, Noboru Mizushima
Summary: Conjugation of Atg8-family proteins to phosphatidylethanolamine (PE) is important for auto-phagosome formation. PE conjugation was previously thought to be specific to Atg8 among the ubiquitin-family proteins, but our recent study found that ubiquitin is also conjugated to PE on endosomes and the vacuole. Other ubiquitin-like proteins, like NEDD8 and ISG15, can also bind to phospholipids covalently. We propose that conjugation to phospholipids could be a common feature of the ubiquitin family.
Article
Chemistry, Multidisciplinary
Ivan Hung, Wenping Mao, Eric G. Keeler, Robert G. Griffin, Peter L. Gor'kov, Zhehong Gan
Summary: High sensitivity and resolution solid-state NMR methods are developed for selecting hydrogen-bonded N-15-O-17 pairs in peptides, aiding protein secondary and tertiary structure determination. Enhanced sensitivity is achieved through indirect H-1 detection under fast MAS and stronger relayed dipole couplings.
CHEMICAL COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Zongang Liu, Mengnan Zhao, Xizi Jiang, Yao Zhang, Suning Zhang, Yitong Xu, Hongjiu Ren, Hongbo Su, Huanxi Wang, Xueshan Qiu
Summary: This study elucidates the role of KLHL17 in the development and progression of NSCLC using clinical samples and NSCLC cell lines. The results show that upregulated KLHL17 in NSCLC promotes the proliferation and migration of tumor by activating Ras/MAPK signaling pathway, and suggest that KLHL17 may be a novel therapeutic target for the treatment of NSCLC.
LABORATORY INVESTIGATION
(2022)
Article
Chemistry, Medicinal
Serah W. Kimani, Julie Owen, Stuart R. Green, Fengling Li, Yanjun Li, Aiping Dong, Peter J. Brown, Suzanne Ackloo, David Kuter, Cindy Yang, Miranda MacAskill, Stephen Scott MacKinnon, Cheryl H. Arrowsmith, Matthieu Schapira, Vijay Shahani, Levon Halabelian
Summary: DCAF1 serves as a subunit for RING-type CRL4(DCAF1) and HECT family EDVPDCAF1 E3 ubiquitin ligases in substrate recruitment. The WDR domain of DCAF1 acts as a binding platform for substrate proteins and is targeted by HIV and SIV lentiviral adaptors. This study used a proteome-scale drug-target interaction prediction model to identify ligands for the DCAF1 WDR domain through biophysical screening and X-ray crystallography, confirming the selective binding of a predicted ligand. The findings demonstrate the successful application of artificial intelligence-enabled virtual screening methods in the absence of previously known ligands.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Thermodynamics
Adam Smolinski, Karolina Wojtacha-Rychter, Magdalena Krol, Malgorzata Magdziarczyk, Jaroslaw Polanski, Natalia Howaniec
Summary: The paper explores the application of the gasification technology of refuse-derived fuels (RDF) with bituminous coal in hydrogen production and circular economy development. The research findings indicate that an increase in the proportion of RDF in the fuel blend leads to a decrease in the total gas yield and hydrogen yield. RDF2, with a higher calcium concentration, exhibits a higher H2 yield during co-gasification with coal.
Article
Chemistry, Analytical
Jiali Qiang, Zhimin Xu, Yunxia Li, Hongbin Wang, Yaoyang Zhang
Summary: Disulfide bonds play a crucial role in regulating protein oxidation status, structure, and stability. The CADI strategy efficiently identifies disulfide bonds in peptides, although it has limitations in achieving in-depth coverage in complex mammalian cell lysates.
ANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Frederik Eisele, Anna Maria Eisele-Burger, Xinxin Hao, Lisa Larsson Berglund, Johanna L. Hoog, Beidong Liu, Thomas Nystrom
Summary: This paper demonstrates the role of the essential Hsp90 co-chaperone Sgt1 in a general protein quality control network, participating in the degradation of misfolded proteins and accumulating in response to proteostatic stress. Sgt1 is involved in a parallel pathway to ubiquitin ligase Hul5 and plays a role in clearing misfolded disease proteins, such as synphilin-1 linked to Parkinson's disease, from quality control sites.
Review
Biochemistry & Molecular Biology
Jin-Taek Hwang, Ahyoung Lee, Changwon Kho
Summary: Post-translational modification (PTM) is an essential mechanism for enhancing the functional diversity of proteins and adjusting their signaling networks. The reversible conjugation of ubiquitin (Ub) and ubiquitin-like proteins (Ubls) to cellular proteins is among the most prevalent PTM, which modulates various cellular and physiological processes by altering the activity, stability, localization, trafficking, or interaction networks of its target molecules. The dysregulation of Ub/Ubl modifications is associated with various diseases, providing new targets for drug development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Computer Science, Artificial Intelligence
Xuan Yang, Wei Wu, George Q. Huang
Summary: This paper proposes a crowdsourced co-modality transportation system (CCTS) to integrate passenger and freight flows into a single system. Empirical investigations are conducted to collect system design considerations, and an overview of the crowdsourced co-modality management platform (CCMP) is presented. The experimental studies show that crowdsourced co-modality is more feasible and promising if more passengers are willing to provide crowdsourcing services.
ADVANCED ENGINEERING INFORMATICS
(2023)
Article
Biochemistry & Molecular Biology
Matthew John Eastham, Andria Pelava, Graeme Raymond Wells, Nicholas James Watkins, Claudia Schneider
Summary: Two of the four human ubiquitin-encoding genes express ubiquitin as an N-terminal fusion precursor polypeptide, with either RPS27a or RPL40 at the C-terminus. RPS27a and RPL40 are important for the induction of p53, suggesting their role in the coordination of ribosome production, ubiquitin levels, and p53 signaling. Knockdown of both RPS27a and RPL40 induces p53 and is needed for rRNA production in MCF7 and LNCaP cells, indicating their cell type-dependent role in p53 signaling and consistent role in ribosome biogenesis.
Article
Chemistry, Physical
Madeleine O. Sattler, Tomilola J. Ajayi, Eric C. Keske, Alan J. Lough, Robert H. Morris
Summary: We synthesized a new Mn-PNNP complex with a new approach for the synthesis of a previously reported PNNP ligand. The complex was characterized by various spectroscopic techniques and X-ray diffraction analysis, which confirmed its distorted octahedral geometry with a rare cis-& alpha;-configuration. The complex showed a large HOMO to LUMO gap and important intermolecular interactions. A preliminary catalytic study of the complex on transfer hydrogenation of acetophenone was conducted.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
