Article
Chemistry, Multidisciplinary
Yingxian Zhang, Jincheng Zhong, Hui Cheng, Jie Huang, Zhenhui Li, Chi Zhang, Zhiang Gao, Zhourui Xu, Gaixia Xu, Ping Qiu, Ke Wang
Summary: By detailed measurements and comparison of the effective attenuation length (l(e)) in different excitation wavelengths, this study demonstrates that 3-photon microscopy at 1700 nm enables the deepest penetration in brain imaging in vivo.
NANOSCALE ADVANCES
(2023)
Article
Oncology
Alexa R. Heaton, Peter R. Rehani, Anna Hoefges, Angelica F. Lopez, Amy K. Erbe, Paul M. Sondel, Melissa C. Skala
Summary: Metabolic reprogramming plays a significant role in tumorigenesis and cancer progression. However, current techniques for measuring tumor and immune cell metabolism lack spatial context. In this study, a mouse model and an algorithm for single cell imaging of immune and cancer cells were developed. The results showed metabolic changes in tumor infiltrating immune cells compared to immune cells from spleens, as well as single cell metabolic heterogeneity in both immune and tumor cells.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Andrea Ceja-Fdez, Ramon Carriles, Ana Lilia Gonzalez-Yebra, Juan Vivero-Escoto, Elder de la Rosa, Tzarara Lopez-Luke
Summary: Gold nanoparticles were prepared and their interaction with HPV and cancerous cervical tissues were studied, showing distribution, light absorption, and Raman spectral characteristics inside tissues. Potential for rapid cancer screening was suggested based on the different spectral signatures observed between HPV-infected and carcinogenic tissues.
Article
Chemistry, Analytical
Palma Anker, Luca Fesus, Norbert Kiss, Judit Noll, Krisztina Becker, Eniko Kuroli, Balazs Mayer, Szabolcs Bozsanyi, Kende Lorincz, Ilze Lihacova, Alexey Lihachev, Marta Lange, Norbert Wikonkal, Marta Medvecz
Summary: This study investigated a rare form of keratinopathic ichthyosis caused by KRT1 mutation using two different optical imaging methods, revealing the usefulness of autofluorescence signal and diffuse reflectance for assessing skin status and nonlinear optical microscopy for visualizing epidermal keratin and dermal collagen. These imaging modalities were found to be valuable for the diagnosis and follow-up of keratinopathic diseases.
Review
Immunology
Philippe Bousso, Capucine L. L. Grandjean
Summary: Modulating the immune system can be used to treat various diseases and also improve our understanding of immune responses. This review discusses how imaging techniques can help clarify the mechanism of action of immunomodulatory strategies and guide the development of new drugs.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Physics, Applied
Kevin Keomanee-Dizon, Matt Jones, Peter Luu, Scott E. Fraser, Thai V. Truong
Summary: ExD-SPIM is an improved light-sheet microscopy strategy that solves the limitation of capturing fluorescence signals in large volumes by extending the depth of field (DOF) of high-NA detection objectives to match the thickness of the illumination light sheet. Compared to conventional light sheet imaging, ExD-SPIM improves the signal-to-noise ratio and reduces the rate of photobleaching. In whole-brain activity imaging, ExD-SPIM enhances signal sensitivity and volumetric coverage.
APPLIED PHYSICS LETTERS
(2022)
Review
Dermatology
Ke-Jun Chen, Yang Han, Zi-Yi Wang, Yong Cui
Summary: Non-invasive optical examination is vital in dermatology for diagnosis, management, and research. Multiphoton microscopy uses submicron technology to stimulate autofluorescence and provides insights into cellular structure, redox status, and collagen fibers in the skin. This review highlights the principles and application of multiphoton microscopy in studying various skin conditions.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Maria Calvo-Rodriguez, Elizabeth K. Kharitonova, Brian J. Bacskai
Summary: Mitochondria play crucial roles in neuronal function, with Ca2+ handling being essential for energy production and cellular fate. Dysfunction in mitochondrial Ca2+ handling has been linked to various neurodegenerative diseases. Investigating mitochondrial Ca2+ dynamics is vital for understanding the role of intracellular Ca2+ signals in physiology and pathology.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Multidisciplinary Sciences
V. Elagin, E. Gubarkova, O. Garanina, D. Davydova, N. Orlinskaya, L. Matveev, I. Klemenova, I. Shlivko, M. Shirmanova, E. Zagaynova
Summary: This study demonstrated the potential of combined use of multiphoton microscopy and optical coherence angiography for improved early diagnosis of melanoma by successfully differentiating equivocal melanocytic lesions.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Chih-Ju Lin, Sohidul Mondal, Sheng-Lin Lee, Jeon-Woong Kang, Peter T. C. So, Chen Yuan Dong
Summary: The formation rate of fluorescent advanced glycation end products (fAGEs) was measured in different glycated porcine tissues (aorta, cornea, kidney, dermis, and tendon) using multiphoton microscopy. Fructose exhibited the highest glycation rate, followed by galactose and glucose. Collagen glycated at a significantly higher rate than non-collagenous tissues. However, the rate of fAGE synthesis varied among collagen in different tissues.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Chemistry, Multidisciplinary
Hans Georg Breunig, Karsten Koenig
Summary: This study investigated the time-resolved two-photon excited autophosphorescence of non-labeled biological specimens for the first time, using phosphoresce lifetime imaging with microscopic spatial resolution. The results showed a strong dependence of the phosphorescence intensity on exposure temperature, with a bi-exponential signal decay observed. The recording of fluorescence and phosphorescence allowed unbiased measurement of the temperature dependence. The measurements on thermally altered bones are particularly relevant to forensic and archeological investigations.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemical Research Methods
A. B. Y. Joseph, Derek Power, Jesse Schallek
Summary: Microglia, essential immune cells in the CNS and retina, were studied in healthy mice using innovative imaging techniques. The research revealed the dynamic behavior of microglial processes at various levels, providing insights into their responses under different conditions. Additionally, a label-free imaging method was demonstrated, offering a new opportunity to study the CNS immune system noninvasively.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Oncology
Paula Patricia Villarreal, Rahul Pal, Suimin Qiu, Orly Coblens, Alejandro Villasante-Tezanos, Vicente Resto, Susan McCammon, Gracie Vargas
Summary: This study evaluates the method of multiphoton autofluorescence microscopy (MPAM) for its ability to reveal atypia associated with neoplasia in resected tumor samples without the need for exogenous dyes or tissue sectioning. MPAM can identify cellular and microstructural atypia in head and neck tissues, and allows for the identification of cellular heterogeneity across transitional areas and other features.
Article
Multidisciplinary Sciences
Juo-Nang Liao, Wei-Liang Chen, Chao-Yuan Lo, Man-Hong Lai, Huang-Lung Tsai, Yu-Ming Chang
Summary: In this study, a nondestructive method using two-photon fluorescence and second-harmonic generation imaging was demonstrated to quantify starch granules within chloroplasts of intact leaves across a day-night cycle. The results provided micron scale spatial resolution of starch distribution within leaves and showed starch circadian patterns consistent with previous studies. This method is a potential tool for revealing the real-time heterogeneity of starch circadian rhythm in leaf cells without the need for destructive sample preparation.
SCIENTIFIC REPORTS
(2022)
Review
Engineering, Biomedical
Irene Georgakoudi, Kyle P. Quinn
Summary: In the past fifty years, the autofluorescence of NADH and FAD has been studied in various cell types and diseases. With the advancement of nonlinear optical microscopy in biomedical research, NADH and FAD imaging provides a noninvasive method to monitor cell and tissue status and understand dynamic changes in metabolism. Different tools and methods have been developed to measure the properties of NADH and FAD autofluorescence, but further work is needed to fully understand metabolism. This article discusses the current understanding of our optical sensitivity to metabolic pathways and the challenges in the field.
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING
(2023)
Article
Instruments & Instrumentation
Rekha Gautam, Deandra Peoples, Kiana Jansen, Maggie O'Connor, Giju Thomas, Sandeep Vanga, Isaac J. Pence, Anita Mahadevan-Jansen
APPLIED SPECTROSCOPY
(2020)
Article
Biochemical Research Methods
Anouk L. Post, Albert J. de Groof, Xu U. Zhang, Anne-Fre Swager, Kiki N. Fockens, Roos E. Pouw, Bas L. A. M. Weusten, Dirk J. Faber, Daniel M. de Bruin, Jacques J. G. H. M. Bergman, Ton G. van Leeuwen, Henricus J. C. M. Sterenborg, Wouter L. Curvers
Summary: This study utilized multidiameter single fiber reflectance spectroscopy (MDSFR) to improve surveillance of Barrett's esophagus, developing a classifier based on logistic regression analysis to predict the presence of neoplasia in other parts of the Barrett's segment with high sensitivity and specificity.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Biochemical Research Methods
Anouk L. Post, Dirk J. Faber, Henricus J. C. M. Sterenborg, Ton G. van Leeuwen
Summary: The study developed a model for spectral measurements and validated it through simulations. Experimental validation was conducted to assess the model's feasibility, and a parameterization method was proposed to prevent overfitting. The parameterization improved fitting speed and enabled single fiber measurements.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Biochemical Research Methods
Emmanuel A. Mannoh, Logan B. Parker, Giju Thomas, Carmen C. Solorzano, Anita Mahadevan-Jansen
Summary: A new imaging system combining near infrared autofluorescence detection and laser speckle contrast imaging is able to simultaneously identify parathyroid glands and assess their vascularity. An automated segmentation algorithm reduces barriers to clinical translation, demonstrating equivalent results to manual segmentation in ex vivo tissue samples.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Surgery
Giju Thomas, Carmen C. Solorzano, Naira Baregamian, Emmanuel A. Mannoh, Rekha Gautam, Rebecca T. Irlmeier, Fei Ye, Jon A. Nelson, Samuel E. Long, Paul G. Gauger, Alexa Magner, Tyler Metcalf, Lawrence A. Shirley, John E. Phay, Anita Mahadevan-Jansen
Summary: The study found that NIRAF detection using PTeye (R) has a significant advantage in aiding parathyroid gland identification compared to a surgeon's own ability.
AMERICAN JOURNAL OF SURGERY
(2021)
Article
Oncology
Colleen M. Kiernan, Giju Thomas, Naira Baregamian, Carmen C. Solorzano
Summary: The study demonstrated that using PTeye (TM) for intraoperative parathyroid gland (PG) identification achieved an overall accuracy of 94.3% and increased confidence among high-volume surgeons. The three participating surgeons all observed this effect when using PTeye (TM).
JOURNAL OF SURGICAL ONCOLOGY
(2021)
Article
Surgery
Carmen C. Solorzano, Giju Thomas, Eren Berber, Tracy S. Wang, Gregory W. Randolph, Quan-Yang Duh, Frederic Triponez
Summary: Near-infrared fluorescence detection has been widely recognized for identifying or preserving parathyroid glands during thyroid and parathyroid operations, with two main approaches: using near infrared auto-fluorescence to locate parathyroid glands, and using contrast-enhanced near-infrared fluorescence to evaluate gland perfusion.
Article
Endocrinology & Metabolism
Emmanuel A. Mannoh, Giju Thomas, Naira Baregamian, Sarah L. Rohde, Carmen C. Solorzano, Anita Mahadevan-Jansen
Summary: Accurate assessment of parathyroid gland vascularity using LSCI is crucial in thyroidectomy. This study found that a speckle contrast value above 0.186 during surgery may be associated with low postoperative PTH levels.
Article
Obstetrics & Gynecology
Laura E. Masson, Christine M. O'Brien, Rekha Gautam, Giju Thomas, James C. Slaughter, Mack Goldberg, Kelly Bennett, Jennifer Herington, Jeff Reese, Emad Elsamadicy, J. Michael Newton, Anita Mahadevan-Jansen
Summary: This study demonstrated that Raman spectroscopy can be used to monitor biochemical changes in the cervix during labor. The technique successfully detected changes in collagen, blood, and lipid components, providing a potential noninvasive tool for objectively evaluating the cervical state and guiding clinical management of labor.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Esther Kho, Behdad Dashtbozorg, Joyce Sanders, Marie-Jeanne T. F. D. Vrancken Peeters, Frederieke van Duijnhoven, Henricus J. C. M. Sterenborg, Theo J. M. Ruers
Summary: Developing algorithms for analyzing hyperspectral images as an intraoperative tool for margin assessment during breast-conserving surgery requires a dataset with reliable histopathologic labels. By comparing tissue slices and lumpectomy datasets, using wavelengths with appropriate penetration depth to develop tissue classification algorithms was explored. Spectral differences between tissue slices and lumpectomy datasets were observed, affecting the discrimination of different tissue types.
APPLIED SCIENCES-BASEL
(2021)
Article
Surgery
Giju Thomas, Colleen M. Kiernan, Parker A. Willmon, Ezekiel Haugen, Amy N. Luckenbaugh, Daniel A. Barocas, Naira Baregamian, Anita Mahadevan-Jansen, Carmen C. Solorzano
Summary: This study introduces a novel label-free approach using near-infrared autofluorescence (NIRAF) detection to enhance intraoperative adrenal gland visualization, showing promising results in distinguishing adrenal cortex and surrounding structures, as well as differentiating malignant adrenal tumors from benign and healthy adrenal tissues in a cohort of 55 patients.
WORLD JOURNAL OF SURGERY
(2023)
Article
Surgery
Naira Baregamian, Konjeti R. Sekhar, Evan S. Krystofiak, Maria Vinogradova, Giju Thomas, Emmanuel Mannoh, Carmen C. Solorzano, Colleen M. Kiernan, Anita Mahadevan-Jansen, Naji Abumrad, Michael L. Freeman, Vivian L. Weiss, Jeffrey C. Rathmell, W. Kimryn Rathmell
Summary: By using fine needle aspiration, the researchers have successfully established 3-dimensional endocrine organoid models that accurately simulate the complex tumor microenvironment of thyroid, parathyroid, and adrenal neoplasms, while maintaining cytokine production and near-infrared autofluorescence properties.
Article
Medicine, Research & Experimental
Ryan H. Belcher, Giju Thomas, Parker A. Willmon, Jean-Nicolas Gallant, Naira Baregamian, Monica E. Lopez, Carmen C. Solorzano, Anita Mahadevan-Jansen
Summary: Compared to adult patients, pediatric patients undergoing thyroid surgery are more likely to develop hypoparathyroidism due to inadvertent injury or devascularization of the parathyroid gland. This study evaluates the utility and accuracy of near-infrared-autofluorescence (NIRAF) for parathyroid identification in pediatric patients. The results indicate that NIRAF detection can be a valuable and non-invasive technique for identifying parathyroid glands during neck operations in children.
Article
Chemistry, Analytical
Giju Thomas, Sean T. T. Fitzgerald, Rekha Gautam, Fuyao Chen, Ezekiel Haugen, Pratheepa Kumari Rasiah, Wilson R. R. Adams, Anita Mahadevan-Jansen
Summary: Biochemical insights into varying breast cancer phenotypes can be obtained through Raman spectroscopy, which provides high specificity in measuring the biochemical differences. In this study, stainless steel substrates were found to deliver stronger Raman signals and improved spectral characterization compared to CaF2 substrates.
ANALYTICAL METHODS
(2023)
Article
Oncology
Lisanne L. de Boer, Esther Kho, Koen K. van de Vijver, Marie-Jeanne T. F. D. Vranken Peeters, Frederieke van Duijnhoven, Benno H. W. Hendriks, Henricus J. C. M. Sterenborg, Theo J. M. Ruers
Summary: DRS has the potential to guide surgeons intraoperatively by characterizing tissue and discriminating between healthy and malignant tissues, including diseases like ductal carcinoma in situ. However, further research is needed to fully understand its optical characteristics and capabilities in characterizing mixed tissue types.
BREAST CANCER RESEARCH
(2021)
