Article
Chemistry, Physical
Anthony V. Sica, Ash Sueh Hua, Helen H. Lin, Ellen M. Sletten, Timothy L. Atallah, Justin R. Caram
Summary: We demonstrate a method called Spectrally selective Time-resolved Emission through Fourier-filtering (STEF) for separating and resolving the dynamics of multiple emitters without using conventional filters. Our approach utilizes a fixed path-length imbalanced Mach-Zehnder interferometer to interfere and cancel (or enhance) specific spectral signatures corresponding to one emissive species. We show the range and utility of STEF by performing various procedures, including filtering laser scatter, separating fluorescence lifetimes, and resolving overlapping fluorescent stains.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Biochemical Research Methods
Alba Alfonso-Garcia, Silvia Noble Anbunesan, Julien Bec, Han Sung Lee, Lee-Way Jin, Orin Bloch, Laura Marcu
Summary: A label-free fluorescence lifetime imaging (FLIm) device was used to evaluate the infiltrative edge of glioblastoma in vivo, and the results support the feasibility of intraoperative FLIm for real-time brain measurements and encourage further refinement to predict the infiltrative edge of glioblastoma.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Optics
Patrick J. Gasda, Roger C. Wiens, Adriana Reyes-Newell, Kumkum Ganguly, Raymond T. Newell, Charles Peterson, Benigno Sandoval, Logan Ott, Samantha Adikari, Seychelles Voit, Samuel M. Clegg, Anupam K. Misra, Tayro E. Acosta-Maeda, Heather Quinn, Shiv K. Sharma, Magdalena Dale, Steven P. Love, Sylvestre Maurice
Summary: OrganiCam is a laser-induced luminescence imager and spectrometer designed for standoff organic and biosignature detection on planetary bodies. It covers a large area and records luminescence, as well as Raman and fluorescence spectra.
Article
Biochemistry & Molecular Biology
Lukasz Saletnik, Wojciech Szczesny, Jakub Szmytkowski, Jacek J. J. Fisz
Summary: This study systematically investigates the fluorescence spectra of collagen isolated from bovine Achilles tendon under steady-state and time-resolved conditions. The shape and position of the measured fluorescence spectra of collagen strongly depend on the excitation and emission wavelengths, indicating the presence of unidentified collagen cross-links. Time-resolved fluorescence studies suggest energy transfer processes between amino acids and collagen cross-links, as well as between cross-links themselves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physics, Multidisciplinary
Marta Ghirardello, Alessia Candeo, Benedetto Ardini, Gianluca Valentini, Cristian Manzoni, Thomas Calligaro, Laurent Pichon, Xueshi Bai, Roland Lenz, Roberto Alberti, Michele Gironda, Daniela Comelli
Summary: UV-Vis-induced luminescence techniques are commonly used for non-invasive examination of cultural heritage objects. In this study, a combination of photoluminescence lifetime imaging and time-gated hyperspectral imaging was proposed to estimate the lifetime of emitters and reconstruct their spectral emission in paintings. This approach allows for the effective identification and mapping of weak luminescent pigments in artworks.
EUROPEAN PHYSICAL JOURNAL PLUS
(2023)
Article
Surgery
Fares Benmiloud, Guillaume Penaranda, Laurent Chiche, Stanislas Rebaudet
Summary: The Fluogreen study aimed to improve vascular preservation of parathyroid glands during thyroid surgery using ICG-based intraoperative mapping angiograms. The study demonstrated that iMAP is feasible and provides direct vascular information in one-third of cases, highlighting its potential to enhance vascular protection for parathyroid glands.
WORLD JOURNAL OF SURGERY
(2022)
Article
Chemistry, Physical
Soo Yeong Lim, Jin Il Jang, Hongman Yoon, Hyung Min Kim
Summary: Autofluorescence imaging has been widely used for noninvasive diagnostics in vivo and ex vivo tissues. The optical redox ratio (ORR), which measures the fluorescence intensity ratio between NADH and FAD, has been used as a diagnostic parameter, particularly in cancer diagnosis. However, there is limited research on the interference between these molecules and its impact on ORR values.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Physics, Multidisciplinary
Mira Sibai, Hussein Mehidine, Bertrand Devaux, Darine Abi Haidar
Summary: Fibred-based optical spectroscopy offers sensitivity, practicality and precision for point of care diagnosis. This study utilizes this advantage to address the limited accuracy in delineating brain tumors by simultaneously characterizing tissue based on spectral and lifetime properties of five endogenous fluorophores. A custom-built 5-meter multi fibre-optic probe with a sterilizable distal end is described, including its technical features such as architecture, collection efficiency, and sensitivity.
FRONTIERS IN PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Wiebke Haselbach, Jeremy M. Kaminski, Laura N. Kloeters, Thomas J. J. Mueller, Oliver Weingart, Christel M. Marian, Peter Gilch, Barbara E. Nogueira de Faria
Summary: The emitters for TADF-based OLEDs need to have small S-1-T-1 energy gaps and fast ISC transitions. Vibronic mixing with higher excited states S-n and T-n can mediate these transitions. By using time-resolved NIR spectroscopy, it is demonstrated that the energetically located higher states can be found in a prototypical TADF emitter consisting of a triarylamine and a dicyanobenzene moiety (TAA-DCN).
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Bo Jiang, Utana Umezaki, Andrea Augustine, Vindi M. Jayasinghe-Arachchige, Leonardo F. Serafim, Zhi Mei Sonia He, Kevin M. Wyss, Rajeev Prabhakar, Angel A. Marti
Summary: In this study, time-resolved spectroscopy was used to investigate the binding interactions between amyloid-beta fibrillar structures and photoluminescent ligands, revealing a novel binding site. Additionally, it was found that common polypyridine complexes can also bind to amyloid-beta at two distinct binding sites. These findings are crucial for understanding the toxicity of proteins and designing potential drugs to mitigate their deleterious effects.
Article
Biochemical Research Methods
Harry Alexander Charles Wood, Katjana Ehrlich, Stephanos Yerolatsitis, Andras Kufcsak, Tom Michael Quinn, Susan Fernandes, Dominic Norberg, Nia Caitlin Jenkins, Vikki Young, Irene Young, Katie Hamilton, Sohan Seth, Ahsan Akram, Robert Rodrick Thomson, Keith Finlayson, Kevin Dhaliwal, James Morgan Stone
Summary: We have developed an endoscopic probe that integrates three optical fibre technologies to enable real-time optical biopsies in the distal lung. The probe combines high-resolution imaging fibre, multimode fibre for fluorescence spectroscopy, and hollow-core fibre for Raman spectroscopy. It has been demonstrated to be flexible enough to navigate in small loops and can be used in human airways with the help of a bronchoscope.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Optics
Alberto Ghezzi, Armin J. M. Lenz, Fernando Soldevila, Enrique Tajahuerce, Vito Vurro, Andrea Bassi, Gianluca Valentini, Andrea Farina, Cosimo D'Andrea
Summary: In this work, we propose a time-resolved multispectral fluorescence microscopy system based on the Single-Pixel Camera (SPC) scheme, which achieves high resolution and short measurement time using Compressive Sensing (CS) technology. Data Fusion (DF) with a high-resolution camera is used to address the low spatial resolution issue typical of SPC. By integrating hardware and algorithms, this system reduces the number of measurements while preserving the information content, allowing for a zoom feature without moving the optical system. The system is characterized in terms of spatial, spectral, and temporal properties, and is validated on a cellular sample.
Article
Oncology
Serkan Akbulut, Ozgun Erten, Mehmet Gokceimam, Yoo S. Kim, Vikram Krishnamurthy, Katherine Heiden, Judy Jin, Allan Siperstein, Eren Berber
Summary: The study compared the capability to detect parathyroid glands (PG) and image quality between older and newer near-infrared imaging (NIFI) technologies. Results showed that the second-generation NIFI (CMOS) displayed higher detection rates and autofluorescence intensity compared to the first-generation technology (CCD).
JOURNAL OF SURGICAL ONCOLOGY
(2021)
Review
Oncology
Yi Zhang, Hongquan Yu, Yunqian Li, Haiyang Xu, Liu Yang, Peilin Shan, Yuejiao Du, Xiaokai Yan, Xuan Chen
Summary: The infiltrative growth and malignant behavior of glioma make it a challenging brain tumor. Maximizing resection while minimizing impact on normal tissue is the goal of neurosurgeons. Current visualization techniques have drawbacks, while Raman spectroscopy offers non-destructive, rapid, and accurate intraoperative glioma identification. This article summarizes the latest research on Raman spectroscopy in glioma surgery.
FRONTIERS IN ONCOLOGY
(2023)
Article
Physics, Multidisciplinary
Alzbeta Marcek Chorvatova, Martin Uherek, Dusan Chorvat
Summary: The study evaluated the effect of nanoparticles from various metals on the endogenous fluorescence of Fontinalis antipyretica moss using confocal microscopy with spectral detection and FLIM. The findings showed that different metals had different impacts on the endogenous fluorescence of the moss, with copper having the most significant effect.
FRONTIERS IN PHYSICS
(2021)
Article
Biochemical Research Methods
Silvia Noble Anbunesan, Alba Alfonso-Garcia, Xiangnan Zhou, Julien Bec, Han Sung Lee, Lee-Way Jin, Orin Bloch, Laura Marcu
Summary: Intraoperative identification of IDH-mutant glioma using fluorescence lifetime imaging can aid in modifying tumor resection strategies for better survival outcomes.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Multidisciplinary Sciences
Ken Miyaguchi, Hongqiang Wang, Keith L. Black, Stephen L. Shiao, Rongfu Wang, John S. Yu
Summary: Efficient activation of T cells with tumor-associated antigens (TAAs) to glioblastoma (GBM) stem cells was achieved through the use of three different antigen sources loaded onto dendritic cells (DCs). An activated T cell (ATC) protocol was developed and optimized for a phase I clinical trial to target GSCs. The synthetic peptide pool loading method showed significantly increased cytotoxicity towards target cells.
SCIENTIFIC REPORTS
(2023)
Article
Clinical Neurology
Yosef Koronyo, Altan Rentsendorj, Nazanin Mirzaei, Giovanna C. Regis, Julia Sheyn, Haoshen Shi, Ernesto Barron, Galen Cook-Wiens, Anthony R. Rodriguez, Rodrigo Medeiros, Joao A. Paulo, Veer B. Gupta, Andrei A. Kramerov, Alexander V. Ljubimov, Jennifer E. Van Eyk, Stuart L. Graham, Vivek K. Gupta, John M. Ringman, David R. Hinton, Carol A. Miller, Keith L. Black, Antonino Cattaneo, Giovanni Meli, Mehdi Mirzaei, Dieu-Trang Fuchs, Maya Koronyo-Hamaoui
Summary: Pathologies of Alzheimer’s disease (AD) can be found in the neurosensory retina, but their exact nature and distribution in relation to disease progression in the brain are still largely unknown. This study conducted extensive histopathological and biochemical investigations of postmortem retina and brain tissues from 86 human donors to better understand the pathological features of AD in the retina. The findings suggest a quantitative relationship between retinopathy, brain pathology, and cognitive impairment, and may lead to the development of reliable retinal biomarkers for noninvasive screening and monitoring of AD.
ACTA NEUROPATHOLOGICA
(2023)
Article
Clinical Neurology
Haoshen Shi, Yosef Koronyo, Dieu-Trang Fuchs, Julia Sheyn, Ousman Jallow, Krishna Mandalia, Stuart L. Graham, Vivek K. Gupta, Mehdi Mirzaei, Andrei A. Kramerov, Alexander V. Ljubimov, Debra Hawes, Carol A. Miller, Keith L. Black, Roxana O. Carare, Maya Koronyo-Hamaoui
Summary: This study found that vascular amyloid beta (Aβ) protein deposits were detected in retinas of mild cognitively impaired (MCI) and Alzheimer's disease (AD) patients, and these deposits were linked to disease status. The study also revealed that the components of retinal vascular tight junctions (TJs) and Aβ expression were correlated with neuropathology. The severe decreases in retinal vascular zonula occludens-1 (ZO-1) and claudin-5 were identified in MCI and AD patients, which correlated with arteriolar Aβ(40) deposition and cerebral pathology and cognitive deficits.
ALZHEIMERS & DEMENTIA
(2023)
Article
Biochemistry & Molecular Biology
Ryan Cordner, Michelle Jhun, Akanksha Panwar, HongQiang Wang, Nicole Gull, Ramachandran Murali, Joseph H. McAbee, Armen Mardiros, Akane Sanchez-Takei, Mia W. Mazer, Xuemo Fan, Emmanuel Jouanneau, John S. Yu, Keith L. Black, Christopher J. Wheeler
Summary: The efficacy of cancer immunotherapy in treating highly malignant tumors like glioblastoma multiforme can be improved by exploiting endogenous immune enhancers among mutated cancer genes. Isocitrate Dehydrogenase-1 (IDH1), commonly mutated in gliomas, enhances glioma vaccine efficacy and can distinguish long-term survivors from short-term survivors in GBM patients. IDH1 enhances T cell responses to tumor antigens and increases glioma cell lysis, while mutant IDH1R132H lacks these effects and decreases host survival after immunotherapy. This discovery uncovers a new axis for immunotherapeutic improvement in GBM and other cancers and reveals novel functions of IDH1, indicating a direct link between lytic T cell function and metabolic activity in target cells.
Review
Physiology
Ron Danziger, Dieu-Trang Fuchs, Yosef Koronyo, Altan Rentsendorj, Julia Sheyn, Eric Y. Y. Hayden, David B. B. Teplow, Keith L. L. Black, Sebastien Fuchs, Kenneth E. E. Bernstein, Maya Koronyo-Hamaoui
Summary: This review discusses the role of angiotensin-converting enzyme (ACE) in Alzheimer's disease (AD) and its potential as a therapeutic target. ACE has been found to degrade the neurotoxic peptide amyloid beta-protein (Aβ(42)), which is associated with AD. Previous studies have shown that overexpression of ACE in myelomonocytic cells improves immune responses and reduces viral and bacterial infection, tumor growth, and atherosclerotic plaque in mice. In a murine model of AD, introducing ACE-expressing myelomonocytes diminished neuropathology and improved cognitive functions through ACE catalytic activity. The therapeutic effects can be achieved by enhancing ACE expression in bone marrow-derived monocytes alone, without targeting central nervous system resident microglia. These findings suggest the potential for utilizing ACE as a natural mechanism to ameliorate AD pathogenesis.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Immunology
Altan Rentsendorj, Koen Raedschelders, Dieu-Trang Fuchs, Julia Sheyn, Vineet Vaibhav, Rebecca A. Porritt, Haoshen Shi, Jargalsaikhan Dagvadorj, Juliana de Freitas Germano, Yosef Koronyo, Moshe Arditi, Keith L. Black, Bhakta Prasad Gaire, Jennifer E. Van Eyk, Maya Koronyo-Hamaoui
Summary: The expression of OPN in macrophages regulates their inflammatory state and protein synthesis. In OPN-deficient macrophages, protein metabolism disruption and apoptosis occur, whereas GA stimulation restores protein synthesis and cellular homeostasis in macrophages.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Engineering, Manufacturing
Jacob Saunders, Mohammad Elbestawi, Qiyin Fang
Summary: Ultrafast lasers have been proven to be evolving manufacturing tools, and additive manufacturing has emerged as a key area for 3D fabrication of objects with arbitrary geometries. The use of ultrafast lasers in additive manufacturing presents possibilities for next-generation manufacturing techniques, particularly for hard-to-process materials, transparent materials, and micro- and nano-manufacturing. This review provides an overview of the development, applications, and recent advancements of selective laser melting/sintering, multiphoton lithography, laser-induced forward transfer, pulsed laser deposition, and welding, as well as the progress in process advancements such as process monitoring and control, increased throughput, and integration into hybrid manufacturing systems.
JOURNAL OF MANUFACTURING AND MATERIALS PROCESSING
(2023)
Editorial Material
Engineering, Electrical & Electronic
Qiyin Fang, Charles Kervrann, David Nolte, Oxana Semyachkina-Glushkovskaya, Shuo Tang, Yuji Matsuura, Zachary Smith
Summary: Biophotonics is an interdisciplinary field that combines photonics and biomedical applications. It has made significant advancements in clinical diagnosis, therapeutics, biotechnology, and environmental studies. The integration of optics and biology has led to quick adoption of photonics technologies in medicine due to its compatibility with minimally invasive procedures.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Liron L. Israel, Tao Sun, Oliver Braubach, Alysia Cox, Ekaterina S. Shatalova, Harun-Mohammad Rashid, Anna Galstyan, Zachary Grodzinski, Xue Ying Song, Oksana Chepurna, Vladimir A. Ljubimov, Antonella Chiechi, Sachin Sharma, Connor Phebus, Yizhou Wang, Julia Y. Ljubimova, Keith L. Black, Eggehard Holler
Summary: Delivery of therapeutic substances to the brain is challenging due to the blood-brain barrier, limited stability, and distribution in brain tissue. However, a study demonstrated efficient delivery of microRNA and antisense RNA specifically to neurons using a biodegradable, amphiphilic, and multivalent platform conjugated with a D3-peptide. This targeting approach, through the transcytosis pathway, shows promise in Alzheimer's disease mouse models.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Neurosciences
Liron L. Israel, Oliver Braubach, Ekaterina S. Shatalova, Oksana Chepurna, Sachin Sharma, Dmytro Klymyshyn, Anna Galstyan, Antonella Chiechi, Alysia Cox, David Herman, Bishop Bliss, Irene Hasen, Amanda Ting, Rebecca Arechavala, Michael T. Kleinman, Rameshwar Patil, Eggehard Holler, Julia Y. Ljubimova, Maya Koronyo-Hamaoui, Tao Sun, Keith L. Black
Summary: In this study using an AD mouse model, the effects of different sized particulate matter from polluted air on Alzheimer's disease development were investigated. The results showed that exposure to airborne PM caused significant dysregulation in the brain transcriptome and accelerated Alzheimer's-related pathology.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Chemistry, Multidisciplinary
Joseph Deering, Jianyu Chen, Dalia Mahmoud, Tengteng Tang, Yujing Lin, Qiyin Fang, Gregory R. Wohl, Mohamed Elbestawi, Kathryn Grandfield
Summary: The newly formed bone structure contains disorder within an ordered multiscale structure from macro to nanoscale. The heterogeneity in mineral ellipsoid packing at the interface of porous titanium implants is characterized using electron microscopy. The ellipsoids within the bone tissue exhibit alternating orientations corresponding to unique lamellar packets near the titanium implant interface, with short-order ellipsoid orientation shifts observed in the 3D probe.
ADVANCED MATERIALS INTERFACES
(2023)
Meeting Abstract
Oncology
Alexander V. Ljubimov, Rameshwar Patil, Hui Ding, Liron Israel, Eggehard Holler, Julia Y. Ljubimova, Tao Sun, Keith L. Black
Article
Oncology
Angelique Sao-Mai S. Tay, Keith L. Black, John S. Yu
Summary: Significant progress has been made in understanding CNS tumors, particularly glioma, but survival rates for malignant glioma patients remain low. Despite advances in radiation and chemotherapy, glioblastoma multiforme (GBM) still has one of the worst 5-year survival rates among all cancers. This chapter reviews the immune system's dysfunction in the tumor microenvironment, identifying potential targets for therapy and discussing current immunotherapy treatment strategies. Additionally, glioma is characterized as a subset of primary spinal cord tumors, and treatment recommendations are outlined.
HUMAN BRAIN AND SPINAL CORD TUMORS: FROM BENCH TO BEDSIDE, VOL. 1: Neuroimmunology and Neurogenetics
(2023)
Article
Clinical Neurology
George K. Hanna, Mecca Madany, Angelique Sao-Mai Sy Tay, Lincoln A. Edwards, Sungjin Kim, Justin S. Michael, Miriam Nuno, Tom Thomas, Aiguo Li, Dror Berel, Keith L. Black, Xuemo Fan, Wei Zhang, Jeremy D. Rudnick, Rongfu Wang, John S. Yu
Summary: The study explores the role of transcription factor ZEB1 in therapeutic resistance to radiation and chemotherapy in glioblastoma. The loss of ZEB1 leads to increased resistance, while overexpression of ZEB1 increases sensitivity. This study uncovers a potential targetable cell surface receptor in these resistant cells.
JOURNAL OF NEUROSURGERY
(2023)