Article
Neurosciences
Vanessa L. Breton, Mark S. Aquilino, Srinivasarao Repudi, Afifa Saleem, Shanthini Mylvaganam, Sara Abu-Swai, Berj L. Bardakjian, Rami Aqeilan, Peter L. Carlen
Summary: Loss of function mutations of the WWOX gene are associated with severe pediatric epileptic encephalopathy. Mouse models show that WWOX plays a crucial role in regulating neuronal excitability.
NEUROBIOLOGY OF DISEASE
(2021)
Review
Neurosciences
Joao Vasco Ferreira, Ana da Rosa Soares, Paulo Pereira
Summary: Aging is associated with the accumulation of proteotoxic material inside cells, which may contribute to neurodegenerative diseases such as Alzheimer's and Parkinson's. Despite efforts to develop therapies targeting proteotoxic accumulation, progress has been limited, as evidenced by the failure of clinical trials for Alzheimer's disease treatments. It is increasingly recognized that regulation of proteostasis involves complex transcellular networks and may shed new light on aging and disease mechanisms.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Computer Science, Information Systems
David F. Parks, Kateryna Voitiuk, Jinghui Geng, Matthew A. T. Elliott, Matthew G. Keefe, Erik A. Jung, Ash Robbins, Pierre Baudin, Victoria T. Ly, Nico Hawthorne, Dylan Yong, Sebastian E. Sanso, Nick Rezaee, Jess L. Seveston, Spencer T. Seiler, Rob Currie, Alex A. Pollen, Keith B. Hengen, Tomasz J. Nowakowski, Mohammed A. Mostajo-Radji, Sofie R. Salama, Mircea Teodorescu, David Haussler
Summary: The Internet of Things (IoT) is widely used in technology companies but rarely in biology experiments. However, it can greatly benefit cloud biology research through alarm notifications, automation, and real-time monitoring of experiments.
INTERNET OF THINGS
(2022)
Article
Chemistry, Analytical
Xuepeng Dong, Wang Wan, Lianggang Zeng, Wenhan Jin, Yanan Huang, Di Shen, Yulong Bai, Qun Zhao, Lihua Zhang, Yu Liu, Zhenming Gao
Summary: This study reports a new donor-pi-acceptor (D-pi-A) type solvatochromic fluorophore scaffold containing a perfluorophenyl group, which allows for control of emission wavelength and intensity by tuning aryl donors. The fluorophore was used to monitor protein misfolding and aggregation, and the polarity of pathogenic aggregated proteins was quantified using HaloTag technology in live cells. This work provides quantitative clues regarding the chemical information at the disease-related protein interphase, complementing efforts to understand the morphology of pathogenic aggregated proteins.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Federico Dona, Cagakan Ozbalci, Andrea Paquola, Federica Ferrentino, Stephen J. Terry, Elisabeth M. Storck, Gaoge Wang, Ulrike S. Eggert
Summary: The membrane protein stomatin is found to be involved in the cytokinesis step of cell division, even though it is not a lipid biosynthetic enzyme. Depletion of stomatin leads to changes in cellular lipidomes, and exogenous phosphatidylcholines can rescue these defects.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Immunology
Manpreet K. Semwal, Nicholas E. Jones, Ann V. Griffith
Summary: The thymus is crucial for T lymphocyte development, with thymic stromal cells playing an important role. Metabolic regulation of thymic stromal cell function is an emerging area of study.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Qiuluo Liu, Yan Chen, Li Zhou, Haining Chen, Zongguang Zhou
Summary: The intestinal epithelium relies on autophagy to maintain homeostasis, but dysregulation of autophagy can lead to the development of colorectal cancer. The role of autophagy in different phases of CRC has conflicting results, with autophagy sometimes being related to CRC and other times inhibiting its progression. Thus, both inhibition and activation of autophagy have been proposed as therapeutic strategies against CRC.
Article
Physiology
Jianxiang Xue, Linto Thomas, Sathish Kumar Murali, Moshe Levi, Robert A. Fenton, Jessica A. Dominguez Rieg, Timo Rieg
Summary: Intestinal NHE3 has a significant contribution to P-i homeostasis. In contrast to effects described for tenapanor on P-i homeostasis, NHE3(IEC-KO) mice show enhanced, rather than reduced, intestinal P-i uptake.
Article
Oncology
Jonathan J. Park, Adan Codina, Lupeng Ye, Stanley Lam, Jianjian Guo, Paul Clark, Xiaoyu Zhou, Lei Peng, Sidi Chen
Summary: Immunotherapy has revolutionized cancer treatments, but resistance remains a major challenge. This study investigates the genetic interactions between significantly mutated tumor suppressors and immune resistance genes in cancer cells' response to T cell killing. The findings provide insights into potential strategies for targeting clinically relevant cancer mutations using existing agents. The study also presents a technology platform and a double knockout library for studying genetic interactions between cancer mutations and immune resistance pathways.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2022)
Review
Cell Biology
Mikolaj Ogrodnik
Summary: The field of cellular senescence research has expanded rapidly from in vitro studies of aging to animal and clinical research, focusing on the role of senescence markers in aging, disease, and anti-senescence interventions. The presence and role of these markers in cells prior to cell cycle arrest, particularly in replicative aging and in vivo conditions, are discussed. The article also explores disparities in quantifications of senescence markers in vivo and the applicational value of broadening the cellular senescence paradigm.
Review
Endocrinology & Metabolism
Rui Gao, Samuel Acreman, Jinfang Ma, Fernando Abdulkader, Anna Wendt, Quan Zhang
Summary: Glucagon is a crucial hormone for elevating glucose levels and maintaining glucose homeostasis. The secretion of glucagon by pancreatic a-cells is regulated by electrical signals, which play a significant role in glucagon release. Understanding the cellular electrophysiology and factors controlling a-cell excitability and glucagon secretion can provide insights for addressing glucagon secretory defects in diabetes and improving diabetes treatment.
JOURNAL OF ENDOCRINOLOGY
(2023)
Article
Cell & Tissue Engineering
Mason Sweat, Yan Sweat, Wenjie Yu, Dan Su, Riley J. Leonard, Steven L. Eliason, Brad A. Amendt
Summary: The miR-200 family plays a crucial role in regulating the fate and differentiation of epithelial stem cells in the murine lower incisor ectodermal organ. Inhibition of miR-200 family results in an expanded stem cell niche and lack of cell differentiation, highlighting the importance of miR-200 in controlling progenitor cell proliferation and differentiation in vivo.
Article
Neurosciences
Andrew R. Rau, Shane T. Hentges
Summary: This study reveals the importance of the VMH glutamate -> POMC neuron circuit in inhibiting food intake, with glutamate release responding to changes in energy state. The circuit conveys excitatory modulation onto POMC cells, affecting food intake and body mass regulation in response to metabolic changes.
JOURNAL OF NEUROPHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ka Lung Cheung, Anbalagan Jaganathan, Yuan Hu, Feihong Xu, Alannah Lejeune, Rajal Sharma, Cristina I. Caescu, Jamel Meslamani, Adam Vincek, Fan Zhang, Kyung Lee, Nilesh Zaware, Amina Abdul Qayum, Chunyan Ren, Mark H. Kaplan, John Cijiang He, Huabao Xiong, Ming-Ming Zhou
Summary: This study uncovers a previously unrecognized mechanism of self-directed cell type-specific regulation of the master transcription factor Stat3 through its own transcriptional target Hipk2 in Th17 cell differentiation. These findings provide insights into the regulation of Th17 cell immune functions and suggest a therapeutic strategy for developing targeted therapies for Th17-associated inflammatory disorders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Jieyu Liu, Yan Ge, Na Wang, Jing Wen, Wei Wang, Bo Zeng, Gui-Lan Chen
Summary: Establishing stable cell lines is crucial for studying gene function, and this study presents an efficient method used to generate knockout cell lines for the PIEZO1 gene and coexpressing cell lines for TRPC5/GCaMP6f-mCherry, suitable for general cell-culture laboratories.
DNA AND CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kyoung-in Cho, Dosuk Yoon, Minzhong Yu, Neal S. Peachey, Paulo A. Ferreira
CELLULAR AND MOLECULAR LIFE SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
John Y. S. Han, Junzo Kinoshita, Sara Bisetto, Brent A. Bell, Romana A. Nowak, Neal S. Peachey, Nancy J. Philp
Article
Multidisciplinary Sciences
Tobias Strunz, Susette Lauwen, Christina Kiel, Anneke den Hollander, Bernhard H. F. Weber
SCIENTIFIC REPORTS
(2020)
Article
Medicine, Research & Experimental
Elliot H. Choi, Susie Suh, David E. Einstein, Henri Leinonen, Zhiqian Dong, Sriganesh Ramachandra Rao, Steven J. Fliesler, Seth Blackshaw, Minzhong Yu, Neal S. Peachey, Krzysztof Palczewski, Philip D. Kiser
Summary: This study presents a novel RPE-specific knockin mouse line with accurate introduction of the CreER(T2) gene into retinal pigment epithelial cells, demonstrating high efficiency and absolute RPE-specific recombination ability for studying gene function and pathophysiology in the RPE.
Article
Ophthalmology
Cari L. Nealon, Christopher W. Halladay, Tyler G. Kinzy, Piana Simpson, Rachael L. Canania, Scott A. Anthony, David P. Roncone, Lea R. Sawicki Rogers, Jenna N. Leber, Jacquelyn M. Dougherty, Jack M. Sullivan, Wen-Chih Wu, Paul B. Greenberg, Sudha K. Iyengar, Dana C. Crawford, Neal S. Peachey, Jessica N. Cooke Bailey
Summary: This study developed and validated a computable phenotype algorithm to identify primary open-angle glaucoma (POAG) using electronic health record (EHR) and genetic data, demonstrating its feasibility and accuracy in studying complex ocular diseases that require imaging confirmation. The findings suggest that combining EHR and genetic data can improve understanding of ocular diseases, particularly those requiring imaging confirmation.
OPHTHALMIC EPIDEMIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gayle B. Collin, Lanying Shi, Minzhong Yu, Nurten Akturk, Jeremy R. Charette, Lillian F. Hyde, Sonia M. Weatherly, Martin F. Pera, Juergen K. Naggert, Neal S. Peachey, Patsy M. Nishina, Mark P. Krebs
Summary: This study reveals defects in the retinal pigment epithelium (RPE) of tvrm77 mice, which is associated with a mutation in the Slc4a5 gene. The mutation leads to reduced Slc4a5 expression and the presence of alternative splice variants. Additionally, the mutation causes retinal detachment, hypopigmented patches, and retinal folds in the mice, as well as altered RPE electrophysiological responses. These findings highlight the importance of SLC4A5 in the outer blood-retinal barrier.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lauren L. Daniele, John Y. S. Han, Ivy S. Samuels, Ravikiran Komirisetty, Nikhil Mehta, Jessica L. McCord, Minzhong Yu, Yekai Wang, Kathleen Boesze-Battaglia, Brent A. Bell, Jianhai Du, Neal S. Peachey, Nancy J. Philp
Summary: Glucose supply is crucial for the normal functioning and survival of retinal cells. This study demonstrates the essential role of glucose in maintaining and sustaining rod photoreceptor outer segments (OS), with minimal impact on cone photoreceptor cells.
Article
Neurosciences
Cyril G. Eleftheriou, Carlo Corona, Shireen Khattak, Nazia M. Alam, Elena Ivanova, Paola Bianchimano, Yang Liu, Duo Sun, Rupesh Singh, Julia C. Batoki, Glen T. Prusky, J. Jason McAnany, Neal S. Peachey, Carmelo Romano, Botir T. Sagdullaev
Summary: This study on a mouse model of X-linked juvenile retinoschisis reveals that abnormal neuroglial signals appear as early as postnatal day 15, providing insights into disease diagnosis and therapeutic intervention.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Ophthalmology
Andrea R. Waksmunski, Tyler G. Kinzy, Lauren A. Cruz, Cari L. Nealon, Christopher W. Halladay, Piana Simpson, Rachael L. Canania, Scott A. Anthony, David P. Roncone, Lea Sawicki Rogers, Jenna N. Leber, Jacquelyn M. Dougherty, Paul B. Greenberg, Jack M. Sullivan, Wen-Chih Wu, Sudha K. Iyengar, Dana C. Crawford, Neal S. Peachey, Jessica N. Cooke Bailey
Summary: This study assessed the utility of a genetic risk score (GRS) based on 127 variants for primary open-angle glaucoma (POAG) risk stratification in European and African ancestry veterans, as well as its association with documented invasive glaucoma surgery (IGS).
Article
Cell Biology
Thanh Hoang, Dong Won Kim, Haley Appel, Nicole A. Pannullo, Patrick Leavey, Manabu Ozawa, Sika Zheng, Minzhong Yu, Neal S. Peachey, Seth Blackshaw
Summary: This study used genetic lineage tracing, single-cell RNA sequencing, and electroretinogram analysis to show that knocking down Ptbp1 does not lead to neuronal conversion in adult Muller glia. Only a few changes in gene expression are observed, and the glial identity is maintained.
Article
Neurosciences
Gabrielle M. Mey, Kirsten S. Evonuk, McKenzie K. Chappell, Laura M. Wolfe, Rupesh Singh, Julia C. Batoki, Minzhong Yu, Neal S. Peachey, Bela Anand-Apte, Robert Bermel, Daniel Ontaneda, Kunio Nakamura, Kedar R. Mahajan, Tara M. DeSilva
Summary: Thalamic volume is associated with clinical disability in multiple sclerosis (MS), and retinal imaging may serve as an important noninvasive predictor of neurodegeneration in MS.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Paulo A. Ferreira
Summary: Nucleocytoplasmic transport plays a crucial role in various cellular processes and impairments to this transport are associated with neurodegenerative diseases. Dysregulation of nucleocytoplasmic partitioning, regulation of transport receptors and client substrates, and disturbances in liquid-liquid phase separation can lead to the formation of intracellular aggregates. This review highlights the importance of Ranbp2 in nucleocytoplasmic transport and proteostasis, and discusses potential therapeutic strategies for neurodegeneration. Ranbp2 has been shown to be involved in the survival of photoreceptor and motor neurons, making it a promising target for neuroprotective interventions.
Meeting Abstract
Ophthalmology
Elliot H. Choi, Susie Suh, Henri Leinonen, David Einstein, Zhiqian Dong, Steven J. Fliesler, Seth Blackshaw, Neal S. Peachey, Krzysztof Palczewski, Philip David Kiser
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Meeting Abstract
Ophthalmology
Robert Igo, Tyler G. Kinzy, Christopher W. Halladay, Dana C. Crawford, Paul B. Greenberg, Jack M. Sullivan, Steven J. Fliesler, Wen-Chih Wu, Helene Choquet, Karina Patasova, Eric Jorgenson, Pirro G. Hysi, Andrew J. Lotery, Jessica Cooke Bailey, Neal S. Peachey, Sudha K. Iyengar
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Meeting Abstract
Ophthalmology
Jessica Cooke Bailey, Cari L. Nealon, Christopher W. Halladay, Piana Krymskaya, Scott A. Anthony, David P. Roncone, Rachael Canania, Tyler G. Kinzy, Robert Igo, Paul B. Greenberg, Dana C. Crawford, Sudha K. Iyengar, Jack M. Sullivan, Wen-Chih Wu, Neal S. Peachey
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)