Article
Biochemistry & Molecular Biology
Jonas Loetscher, Adria-Arnau Marti Lindez, Nicole Kirchhammer, Elisabetta Cribioli, Greta Maria Paola Giordano Attianese, Marcel P. Trefny, Markus Lenz, Sacha Rothschild, Paolo Strati, Marco Kuenzli, Claudia Lotter, Susanne H. Schenk, Philippe Dehio, Jordan Loeliger, Ludivine Litzler, David Schreiner, Victoria Koch, Nicolas Page, Dahye Lee, Jasmin Graehlert, Dmitry Kuzmin, Anne-Valerie Burgener, Doron Merkler, Miklos Pless, Maria L. Balmer, Walter Reith, Joerg Huwyler, Melita Irving, Carolyn G. King, Alfred Zippelius, Christoph Hess
Summary: This study reveals the importance of extracellular magnesium in cellular immunity, particularly in the activation of the co-stimulatory molecule LFA-1. The findings demonstrate that magnesium-sufficiency enhances the function of immune cells and the effectiveness of T cell therapies. Low serum magnesium levels are associated with disease progression and reduced overall survival in patients receiving CAR T cell and immune checkpoint antibody treatments.
Article
Multidisciplinary Sciences
Valentina Botero, Bethany A. Stanhope, Elizabeth B. Brown, Eliza C. Grenci, Tamara Boto, Scarlet J. Park, Lanikea B. King, Keith R. Murphy, Kenneth J. Colodner, James A. Walker, Alex C. Keene, William W. Ja, Seth M. Tomchik
Summary: Neurofibromatosis type 1 is a genetic disorder that affects metabolic homeostasis through a discrete neuronal circuit, leading to increased metabolic rate, feeding, and alterations in lipid stores and turnover kinetics.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Hao-ze Shi, Jing-shu Xiong, Lu Gan, Ying Zhang, Cong-cong Zhang, Ying-qi Kong, Qiu-ju Miao, Cui-cui Tian, Rong Li, Jin-quan Liu, Er-jia Zhang, Wen-bo Bu, Yan Wang, Xian-feng Cheng, Jian-fang Sun, Hao Chen
Summary: This study found that m6A reader YTHDF3 plays an important role in the metastasis of melanoma. The downstream target of YTHDF3, LOXL3, was identified, and it was confirmed that YTHDF3 regulates the transcript of LOXL3 through m6A binding sites. Overexpression of LOXL3 weakened the inhibitory effect of YTHDF3 downregulation on melanoma metastasis.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Suman Shrestha, A. Clay Clark
Summary: Caspases are a family of cysteinyl proteases that control programmed cell death and maintain homeostasis in multi-cellular organisms. The apoptotic caspases evolved from a common ancestor (CA) into two distinct subfamilies: monomers (initiator caspases) or dimers (effector caspases). Differences in activation mechanisms of the two subfamilies, and their oligomeric forms, play a central role in the regulation of apoptosis. The folding landscape established with the common ancestor has been retained for over 650 million years, providing mechanisms for evolutionary changes that affect stability of extant caspases.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Emily D. Duncan, Ke-Jun Han, Margaret A. Trout, Rytis Prekeris
Summary: In this study, the authors demonstrate that the Rab40b/Cullin5 complex ubiquitylates and regulates Rap2, which is crucial for actin dynamics and cell migration in breast cancer cells.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ana Paula Zen Petisco Fiore, Ana Maria Rodrigues, Helder Veras Ribeiro-Filho, Antonio Carlos Manucci, Pedro de Freitas Ribeiro, Mayara Carolinne Silva Botelho, Christine Vogel, Paulo Sergio Lopes-de-Oliveira, Michele Pagano, Alexandre Bruni-Cardoso
Summary: This study uncovers the biochemical mechanisms controlling MST2 degradation and highlights the regulation of proteostasis of a central Hippo pathway component by alterations in the microenvironment rigidity.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Article
Immunology
Yao Chen, Ryan A. Zander, Xiaopeng Wu, David M. Schauder, Moujtaba Y. Kasmani, Jian Shen, Shikan Zheng, Robert Burns, Elizabeth J. Taparowsky, Weiguo Cui
Summary: During chronic viral infection, distinct subsets of effector CD8(+) T cells can arise, including progenitors, exhausted cells, and cytotoxic effector cells. The differentiation of CX(3)CR1(+) effector cells is regulated by Tbx21 and BATF transcription factors.
Article
Multidisciplinary Sciences
Pongdet Piromyou, Hien P. Nguyen, Pongpan Songwattana, Pakpoom Boonchuen, Kamonluck Teamtisong, Panlada Tittabutr, Nantakorn Boonkerd, Piyada Alisha Tantasawat, Michael Goettfert, Shin Okazaki, Neung Teaumroong
Summary: The symbiosis between Vigna radiata and Bradyrhizobium diazoefficiens USDA110 is determined by the type III effector NopE, which triggers incompatibility with certain varieties of V. radiata but promotes nodulation in others. NopE is crucial for early infection and nodule organogenesis in compatible plants, and it is highly conserved among Bradyrhizobia and other plant-associated bacteria. The findings suggest that NopE may be used by V. radiata and B. diazoefficiens USDA110 to optimize their symbiotic interactions by suppressing salicylic acid biosynthesis and reducing phytohormone-mediated responses.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Yan-Bo Zhao, Meng-Xi Liu, Tao-Tao Chen, Xiaomin Ma, Ze-Kai Li, Zichao Zheng, Si-Ru Zheng, Lifei Chen, You-Zhi Li, Li-Rui Tang, Qi Chen, Peiyi Wang, Songying Ouyang
Summary: This study reveals the direct recognition and activation mechanism of the plant CNL Sr35, which can directly recognize the pathogen effector AvrSr35 and trigger immune response. The assembly of Sr35 resistosome is related to the direct recognition of the leucine-rich repeat domain of Sr35.
Article
Cell Biology
Luning Wang, Xiaohong Zhang, Junxiu Sheng, Lei Chen, Lili Zhi, Yangfan Qi, Qianqian Zheng, Linlin Wang, Jinrui Zhang, Jinyao Zhao, Yang Wang, Shu-Xin Liu, Ming-Zhong Sun, Wenjing Zhang
Summary: Cellular senescence is a powerful mechanism for inhibiting the proliferation of cancer cells, and RNA-binding proteins (RBPs) play important roles in regulating senescence. This study discovered that the RBP RBM4 contributes to cellular senescence and its depletion induces senescence in various cell types, including cancer cells. Furthermore, the depletion of RBM4 inhibits cancer cell progression and is mediated by the regulation of SERPINE1 through miR-1244.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Chun-Wei Lin, Laura M. Nocka, Brittany L. Stinger, Joseph B. DeGrandchamp, L. J. Nugent Lew, Steven Alvarez, Henry T. Phan, Yasushi Kondo, John Kuriyan, Jay T. Groves
Summary: This study demonstrates that the protein condensation phase transition of EGFR:Grb2 acts as a regulator of signal propagation from EGFR to the MAPK pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Chun-Wei Lin, Laura M. Nocka, Brittany L. Stinger, Joseph B. DeGrandchamp, L. J. Nugent Lew, Steven Alvarez, Henry T. Phan, Yasushi Kondo, John Kuriyan, Jay T. Groves
Summary: This study demonstrates the importance of a protein condensation phase transition between EGFR and Grb2 in regulating signal propagation from EGFR to the MAPK pathway. The condensation state of the EGFR tail controls the ability of SOS to activate Ras.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
He Sun, Xinlu Zhu, Chuanxi Li, Zhiming Ma, Xiao Han, Yuanyuan Luo, Liang Yang, Jing Yu, Yansong Miao
Summary: The study reveals a mechanism by which bacterial T3Es subvert the host actin cytoskeleton through liquid-liquid phase separation, providing insight into the sophisticated strategies pathogens use to manipulate host cell processes.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Xuedan Wu, Xingbo Cai, Baowen Zhang, Shuting Wu, Ruiju Wang, Na Li, Yunhai Li, Yu Sun, Wenqiang Tang
Summary: ERECTA regulates seed size by regulating the proliferation of outer integument cells through an MKK4/5-MPK3/6-DA1-UBP15 signaling pathway in Arabidopsis. This study uncovers a novel mechanism of ER in seed size regulation, which is independent of its intracellular domain. The identified MAPK signaling pathway downstream of ER, involving MPK3/6, DA1, and UBP15, plays a crucial role in modulating seed size by promoting outer integument cell proliferation.
Article
Biology
Yasmin ElMaghloob, Begona Sot, Michael J. McIlwraith, Esther Garcia, Tamas Yelland, Shehab Ismail
Summary: The study demonstrates that BART acts as a bona fide co-GEF for ARL3, interacting with ARL3 and ARL13B to efficiently activate ARL3 and maintain its active state. This finding contributes to a better understanding of the role of ARL3 in regulating ciliary function.
Article
Biochemistry & Molecular Biology
Thibaut Barnoud, Howard Donninger, Geoffrey J. Clark
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Cell Biology
Howard Donninger, Thibaut Barnoud, Geoffrey J. Clark
Article
Gastroenterology & Hepatology
Payal Arora, Amartya Basu, M. Lee Schmidt, Geoffrey J. Clark, Howard Donninger, Daniel B. Nichols, Diego F. Calvisi, Neerja Kaushik-Basu
Article
Oncology
Dominique Z. Jones, M. Lee Schmidt, Suman Suman, Katharine R. Hobbing, Shirish S. Barve, Leila Gobejishvili, Guy Brock, Carolyn M. Klinge, Shesh N. Rai, Jong Park, Geoffrey J. Clark, Rajesh Agarwal, LaCreis R. Kidd
Article
Oncology
M. Lee Schmidt, Katharine R. Hobbing, Howard Donninger, Geoffrey J. Clark
Article
Biochemical Research Methods
Liqing He, Xiaoli Wei, Xipeng Ma, Xinmin Yin, Ming Song, Howard Donninger, Kavitha Yaddanapud, Craig J. McClain, Xiang Zhang
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2019)
Letter
Respiratory System
Desmond Harrell Stewart, Katherine Hobbing, M. Lee Schmidt, Howard Donninger, Geoffrey J. Clark
JOURNAL OF THORACIC DISEASE
(2019)
Review
Immunology
Chi Li, Howard Donninger, John Eaton, Kavitha Yaddanapudi
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Oncology
Desmond R. Harrell Stewart, M. Lee Schmidt, Howard Donninger, Geoffrey J. Clark
Meeting Abstract
Biochemistry & Molecular Biology
Suman Suman, Dominique Z. Z. Jones-Reed, M. L. Schmidt, Geoffrey J. Clark, Carolyn Klinge, Shirish Barve, Kevin S. Kimbro, La Creis R. Kidd
Review
Oncology
Thibaut Barnoud, M. Lee Schmidt, Howard Donninger, Geoffrey J. Clark