Article
Biochemistry & Molecular Biology
Alicia Otero, Marina Betancor, Hasier Erana, Natalia Fernandez Borges, Jose J. Lucas, Juan Jose Badiola, Joaquin Castilla, Rosa Bolea
Summary: Prion diseases are a group of neurodegenerative disorders caused by the conversion of prion protein to its abnormal isoform. Studies have shown that ER stress and proteasome impairment occur early in spontaneous prion diseases, contributing to the accumulation of misfolded proteins. The findings suggest a strong correlation between ER stress, proteasome impairment and the neuropathological markers of prion diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
John C. Christianson, Pedro Carvalho
Summary: This article discusses recent advances in understanding the mechanisms of ERAD and its impact on the regulation of ER functions.
Review
Biochemistry & Molecular Biology
Ji An Kang, Young Joo Jeon
Summary: The endoplasmic reticulum (ER) is crucial for protein synthesis and quality control, requiring significant resources to maintain protein homeostasis. Misfolded proteins pose a threat to proteostasis, which can be regulated through ERAD and ER-phagy processes in the ER.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Keisuke Mochida, Hitoshi Nakatogawa
Summary: Autophagy is a degradation system in eukaryotic cells that controls the mass and functions of organelles by degrading excess or defective portions. The endoplasmic reticulum (ER) is an organelle with specific functions that dynamically changes its mass and shape to maintain or regulate its functions. Elaborate mechanisms are required for the proper degradation of the ER.
Article
Biochemistry & Molecular Biology
Mikhail Rudinskiy, Maurizio Molinari
Summary: Conserved catabolic pathways operate to remove aberrant polypeptides from the endoplasmic reticulum (ER), utilizing ERAD pathways and ER-phagy. ERAD pathways control retrotranslocation of misfolded proteins across the ER membrane, while ER-phagy segregates proteins into ER subdomains that eventually vesiculate. These ER-derived vesicles can be captured by autophagosomes, endolysosomes/vacuoles, or fused with degradative organelles.
Article
Genetics & Heredity
Kunio Nakatsukasa, Sylvia Wigge, Yuki Takano, Tomoyuki Kawarasaki, Takumi Kamura, Jeffrey L. Brodsky
Summary: This study investigates the function of Hrd1 in yeast to better understand its mechanism. Using a model substrate, Sec61-2, it is found that Hrd1 plays a crucial role in the stability of the substrate and cell viability. Through screening, a group of mutations in HRD1 are identified, disrupting the stability of the Hrd1 complex. These findings confirm the importance of the Hrd1 complex in ERAD.
Article
Biochemistry & Molecular Biology
Kohta Miura, Riko Katsuki, Shusei Yoshida, Ren Ohta, Taku Tamura
Summary: ERAD is an essential process for maintaining cell function and preventing ER stress. EDEM1 was found to be involved in accelerating the degradation of EGFR via ERAD, highlighting its physiological role in maintaining specific target proteins and providing a potential approach to regulate the expression of clinically important proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Gunes Parlakgul, Ana Paula Arruda, Song Pang, Erika Cagampan, Nina Min, Ekin Guney, Grace Yankun Lee, Karen Inouye, Harald F. Hess, C. Shan Xu, Gokhan S. Hotamisligil
Summary: This study reveals the complex structural organization of organelles in liver tissue and highlights the significant alterations in obese mice. The functional importance of these structural changes on cellular and systemic metabolism is also demonstrated.
Article
Plant Sciences
Hyeongjoo Oh, Min-Kyung Kang, Sin-Hye Park, Dong Yeon Kim, Soo-Il Kim, Su Yeon Oh, Woojin Na, Jae-Hoon Shim, Soon Sung Lim, Young-Hee Kang
Summary: Asaronic acid can inhibit cellular stress caused by 7 beta-hydroxycholesterol, decrease the activation of ER stress sensors, and enhance ubiquitin-proteasomal degradation, potentially serving as a potent atheroprotective agent.
Article
Pharmacology & Pharmacy
Narathip Naradun, Krajang Talabnin, Kanyavee Issarangkul Na Ayuttha, Chutima Talabnin
Summary: Cholangiocarcinoma is a lethal malignancy with limited treatment options. Piperlongumine, a biologically active alkaloid, has shown potential as a therapeutic option for cholangiocarcinoma by inducing cancer cell death. Combination treatment with piperlongumine and a proteasome inhibitor further enhances the anti-cancer activity. This research provides valuable insights into the potential use of piperlongumine as an alternative therapy for cholangiocarcinoma.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Environmental Sciences
Yuting Wang, Lei Xu, Lu Peng, Changyu Fang, Qi Qin, Xuying Lv, Zixuan Liu, Bingwei Yang, Erqun Song, Yang Song
Summary: This study demonstrated for the first time that PBDEQ-induced oxidative protein damage in LO2 cells, providing a better understanding of the cytotoxicity of PBDEs and their metabolites.
Review
Physiology
Fulvio Reggiori, Maurizio Molinari
Summary: ER-phagy is the process of degrading portions of the endoplasmic reticulum (ER) within lysosomes or vacuoles. It plays a role in recycling cytoplasmic material and organelles, regulating ER size and activity, and removing potentially cytotoxic material. Dysfunctional ER-phagy is associated with specific human diseases and can be targeted by pathogens.
PHYSIOLOGICAL REVIEWS
(2022)
Article
Neurosciences
Katarina Ziakova, Maria Kovalska, Ivana Pilchova, Katarina Dibdiakova, Maria Brodnanova, Michal Pokusa, Dagmar Kalenska, Peter Racay
Summary: A transient global brain ischemia leads to selective ischemic neurodegeneration, and the mechanism of such selective and delayed neurodegeneration is still uncertain. This study focused on the involvement of proteasomal and endoplasmic reticulum (ER) stress in ischemic neurodegeneration. Laser scanning confocal microscopy analysis was performed on brain slices from animals underwent global brain ischemia. The results suggest dysfunction of the ubiquitin proteasome system and consequent p53-induced expression of PUMA as the main mechanisms responsible for selective and delayed degeneration of hippocampal pyramidal neurons.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Cell Biology
Yanan Li, Shujing Li, Huijian Wu
Summary: Cells adapt to environmental stimuli by making changes to combat injury and increase stress tolerance. If the damage is too severe to repair, cells undergo apoptosis to protect the overall population. Protein aggregation can cause serious damage to the cells, but ubiquitin plays a crucial role in clearing these abnormal proteins. Dysregulation of ubiquitin-proteasome system and autophagy can lead to the development of diseases.
Review
Biochemistry & Molecular Biology
Junyan Qu, Tingting Zou, Zhenghong Lin
Summary: The endoplasmic reticulum (ER) is crucial for protein synthesis and metabolism in eukaryotic cells. When unfolded proteins exceed the ER's folding capacity due to environmental influences or increased protein synthesis, cells activate the endoplasmic reticulum stress (ER stress) response. The components of the ubiquitin-proteasome system (UPS), E3 ubiquitin ligases and deubiquitinases (DUBs), are closely related to ER stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)