Article
Multidisciplinary Sciences
Ana Nikolic, Francesca Maule, Anna Bobyn, Katrina Ellestad, Seungil Paik, Sajid A. Marhon, Parinaz Mehdipour, Xueqing Lun, Huey-Miin Chen, Claire Mallard, Alexander J. Hay, Michael J. Johnston, Christopher J. Gafuik, Franz J. Zemp, Yaoqing Shen, Nicoletta Ninkovic, Katalin Osz, Elodie Labit, N. Daniel Berger, Duncan K. Brownsey, John J. Kelly, Jeff Biernaskie, Peter B. Dirks, Darren J. Derksen, Steven J. M. Jones, Donna L. Senger, Jennifer A. Chan, Douglas J. Mahoney, Daniel D. De Carvalho, Marco Gallo
Summary: The histone variant macroH2A2 controls an epigenetic mechanism of self-renewal and suggests new treatment approaches for glioblastoma patients.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Kun Wang, Shujie Liu, Zhiyuan Dou, Shuo Zhang, Xingsheng Yang
Summary: This study elucidated the cancer stem cell (CSC) properties of multicellular spheroids/aggregates (MCSs/MCAs) derived from ascites in advanced ovarian cancer, showing enhanced malignancy, induced EMT, and low KLF9 expression compared to primary tumor cells (PTCs). KLF9 negatively modulated stem-like properties in OC cells by reducing Notch1 expression, and its loss predicted poor prognosis in ovarian cancer.
Article
Biochemistry & Molecular Biology
Yang Liu, Gang Xu, Haijuan Fu, Peiyao Li, Danyang Li, Kun Deng, Wei Gao, Yujie Shang, Minghua Wu
Summary: LRRC4 is a novel membrane-bound transcription factor that translocates to the nucleus via endoplasmic reticulum-Golgi transport, affecting glioblastoma cell movement. LRRC4 binds to the enhancer element of the RAP1GAP gene to activate its transcription and alters cellular biophysical properties, leading to suppressed tumors. This study provides potential for targeted treatment in glioblastoma.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Oncology
Adam R. Brown, Iad Alhallak, Rosalia C. M. Simmen, Stepan B. Melnyk, Melissa E. Heard-Lipsmeyer, Maria Theresa E. Montales, Daniel Habenicht, Trang T. Van, Frank A. Simmen
Summary: The study revealed that KLF9 acts as a suppressor of liver oxidative stress and inflammation, potentially explaining its tumor-suppressing effects in various tissues.
Article
Biotechnology & Applied Microbiology
Jiahua Xing, Ziqi Jia, Yichi Xu, Muzi Chen, Zheng Yang, Youbai Chen, Yan Han
Summary: Cutaneous squamous cell carcinoma (CSCC) is the second most common skin cancer in humans with increasing incidence. In this study, the researchers focused on the effects of krueppel-like factor 9 (KLF9) on the progression of CSCC cells by binding to PFKFB3. The results showed that PFKFB3 expression was elevated in CSCC cells compared with HaCaT, and its knockdown inhibited the proliferation, metastasis, and aerobic glycolysis of CSCC cells. Additionally, KLF9 was found to bind to PFKFB3, and downregulation of KLF9 compromised the inhibitory effect of PFKFB3 knockdown on CSCC cell proliferation, metastasis, and aerobic glycolysis.
Article
Plant Sciences
Wei-Gui Luo, Qi-Wen Liang, Yi Su, Chao Huang, Bei-Xin Mo, Yu Yu, Lang-Tao Xiao
Summary: Auxin, an important phytohormone in plant, negatively regulates chlorophyll biosynthesis by suppressing the expression of chlorophyll biosynthesis genes such as PORA and GUN5 with the help of IAA14. The yuc2 yuc6 double mutant showed higher chlorophyll content and improved photosynthetic efficiency, indicating the inhibitory role of auxin in chlorophyll metabolism. Our findings provide new insights into the interplay between auxin signaling and chlorophyll metabolism.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Gastroenterology & Hepatology
Tao Li, Yingchun Wan, Ziyuan Su, Jiayu Li, Minna Han, Changyu Zhou
Summary: This study demonstrated that MSC-derived exosomal miR-3940-5p inhibits invasion and EMT of CRC cells, as well as growth and metastasis of tumors, through targeting ITGA6 and subsequently inactivating TGF-beta1.
DIGESTIVE DISEASES AND SCIENCES
(2021)
Article
Oncology
Zhaotao Wang, Yongping Li, Minyi Liu, Danmin Chen, Jiajie Lu, Yunxiang Ji, Zhou Xing, Yezhong Wang
Summary: The study demonstrates that 3BDO can suppress the progression and growth of GBM both in vitro and in vivo by downregulating survivin-mediated stemness and EMT.
Article
Oncology
Elisabetta Stanzani, Leire Pedrosa, Guillaume Bourmeau, Oceane Anezo, Aleix Noguera-Castells, Anna Esteve-Codina, Lorena Passoni, Michela Matteoli, Nuria de la Iglesia, Giorgio Seano, Fina Martinez-Soler, Avelina Tortosa
Summary: The study reveals the crucial role of integrin a6 in controlling stemness and radiotherapy resistance in different molecular subtypes of glioblastoma stem-like cells. Inhibition of integrin a6 can potentially limit malignant characteristics and tumor relapse in both GSCs subtypes, highlighting its importance in improving patient outcomes.
Article
Biochemistry & Molecular Biology
Leonid Anikin, Dimitri G. Pestov
Summary: Aminoacridines, historically used as antiseptic and antiparasitic agents, could be repurposed for therapeutic use and new drug development. In this study, the effects of 9-aminoacridine on pre-rRNA metabolism in mammalian cells were investigated. The results showed that 9-aminoacridine inhibits both pre-rRNA transcription and processing, leading to the disruption of ribosome biogenesis. The ability of 9-aminoacridine to bind to RNA in vitro suggests its potential as a new ribosome biogenesis inhibitor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Weichao Cai, Yecheng Li, Weihong Cao
Summary: The expression of KLF9 is associated with tumor prognosis and immune invasion. Low expression of KLF9 may lead to poor tumor prognosis, while its expression is positively correlated with immune cell infiltration. Therefore, KLF9 may serve as a potential biomarker for tumor prognosis.
Article
Multidisciplinary Sciences
Jin-Kyoung Shim, Seung Hoon Lim, Ji Hye Jeong, Ran Joo Choi, Yoojung Oh, Junseong Park, Sunghee Choi, Junpyo Hong, Seo Jin Kim, Ju Hyung Moon, Eui Hyun Kim, Wan-Yee Teo, Bong Jin Park, Jong Hee Chang, Jae-Ha Ryu, Seok-Gu Kang
Summary: The lignan DFS inhibits the proliferation of GBM tumorspheres by suppressing FOXM1, suggesting its potential as a therapeutic agent for GBM.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Ning Han, Lihong Zhang, Mi Guo, Li Yu
Summary: The current study shows the crucial role of KLF9 in proliferative diabetic retinopathy. Silencing KLF9 can mitigate the progression of PDR by inhibiting angiogenesis through blocking YAP1 transcription.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Urara Tanaka, Shunichi Kajioka, Livia S. Finoti, Daniela B. Palioto, Denis F. Kinane, Manjunatha R. Benakanakere
Summary: This study demonstrated that Decitabine inhibits bone loss and osteoclast differentiation in experimental periodontitis, while upregulating anti-inflammatory cytokines through a KLF2-dependent mechanism. This suggests that DNA methyltransferase inhibitors could be a potential novel therapy for periodontitis.
Article
Pharmacology & Pharmacy
Hua Wang, Yinfeng Tan, Hao Jia, Danqi Liu, Rangru Liu
Summary: In this study, it was found that Posaconazole (POS) has potential antitumor activity against glioblastoma (GBM) and its molecular mechanisms were elucidated. POS can weaken the stemness of CSCs and promote autophagy induction. It was also revealed that POS targets survivin and suppresses the Wnt/β-catenin signaling pathway in GBM. This study provides an experimental foundation for the development of POS as a CSCs-targeting antitumor drug for GBM treatment.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Keila Alvarado-Estrada, Lina Marenco-Hillembrand, Sushila Maharjan, Valerio Luca Mainardi, Yu Shrike Zhang, Natanael Zarco, Paula Schiapparelli, Hugo Guerrero-Cazares, Rachel Sarabia-Estrada, Alfredo Quinones-Hinojosa, Kaisorn L. Chaichana
Summary: Cancer cells circulating in the blood vessels can survive and lead to increased metastatic disease under the pressure of circulation. Specific transition phenotypes of cancer cells play a critical role in their survival and metastatic potential in the circulatory system.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Yolanda Aguilera, Nuria Mellado-Damas, Laura Olmedo-Moreno, Victor Lopez, Concepcion Panadero-Moron, Marina Benito, Hugo Guerrero-Cazares, Catalina Marquez-Vega, Alejandro Martin-Montalvo, Vivian Capilla-Gonzalez
Summary: Utilizing mesenchymal stem cells through intranasal application is a safe and non-invasive strategy for treating neurological disorders, as evidenced by studies conducted on mice. The repeated doses of cells administered through the nostrils did not cause any serious adverse events or tumor formation, demonstrating the potential for future clinical trials. These findings support the use of intranasal MSC therapy for managing central nervous system diseases.
Editorial Material
Clinical Neurology
Rodrigo Ramos-Zuniga, Hugo Guerrero-Cazares, Ulises Gomez-Pinedo, Jorge Matias-Guiu
FRONTIERS IN NEUROLOGY
(2021)
Article
Clinical Neurology
Anteneh M. Feyissa, Anna Carrano, Xue Wang, Mariet Allen, Nilufer Ertekin-Taner, Dennis W. Dickson, Mark E. Jentoft, Steven S. Rosenfeld, William O. Tatum, Anthony L. Ritaccio, Hugo Guerrero-Cazares, Alfredo Quinones-Hinojosa
Summary: In this study, altered processes such as cell-to-cell signaling, inflammation-related, and glutamatergic neurotransmission were identified in the pathogenesis of GRS. DEGs specific to GRS included overexpression of genes involved in cell-to-cell and glutamatergic signaling, and down-regulation of genes involved in immune-trafficking. Target genes identified offer new landscape for further study in the fields of brain tumors and seizures.
Article
Oncology
Karim ReFaey, William D. Freeman, Shashwat Tripathi, Hugo Guerrero-Cazares, Tiffany A. Eatz, James F. Meschia, Rickey E. Carter, Leonard Petrucelli, Fredric B. Meyer, Alfredo Quinones-Hinojosa
Summary: Over the past 23 years, despite an overall increase in NIH grants awarded to neurosurgery departments, the proportion of neurosurgeon-scientists receiving NIH funding compared to scientists has been decreasing. The shift is seen in more funding allocated to senior neurosurgeon-scientists compared to early-career ones, with a focus on neurosurgical oncology-related grants.
JOURNAL OF NEURO-ONCOLOGY
(2021)
Article
Oncology
Cesar A. Garcia, Adip G. Bhargav, Mieu Brooks, Paola Suarez-Meade, Sujan K. Mondal, Natanael Zarco, Karim ReFaey, Mark Jentoft, Erik H. Middlebrooks, Matija Snuderl, Anna Carrano, Hugo Guerrero-Cazares, Paula Schiapparelli, Rachel Sarabia-Estrada, Alfredo Quinones-Hinojosa
Summary: The study functionally characterized six patient-derived GBM cell lines and established sex-specific preclinical models, showing that GBM cell lines of different sexes exhibited differential growth rates and survival times in mice.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Cell Biology
Paula Schiapparelli, Natasha L. Pirman, Kyle Mohler, Pierre A. Miranda-Herrera, Natanael Zarco, Onur Kilic, Chad Miller, Sagar R. Shah, Svetlana Rogulina, William Hungerford, Laura Abriola, Denton Hoyer, Benjamin E. Turk, Hugo Guerrero-Cazares, Farren J. Isaacs, Alfredo Quinones-Hinojosa, Andre Levchenko, Jesse Rinehart
Summary: Genetic code expansion has enabled the production of proteins with specific post-translational modifications, and in this study, a recoded bacterial strain was used to encode phosphoserine into human kinases. This synthetic activation of WNK kinase networks was shown to accurately model cellular systems and could be applied more broadly for research and discovery of phosphorylated protein networks.
Article
Cell Biology
Tulsi Patel, Troy P. Carnwath, Xue Wang, Mariet Allen, Sarah J. Lincoln, Laura J. Lewis-Tuffin, Zachary S. Quicksall, Shu Lin, Frederick Q. Tutor-New, Charlotte C. G. Ho, Yuhao Min, Kimberly G. Malphrus, Thuy T. Nguyen, Elizabeth Martin, Cesar A. Garcia, Rawan M. Alkharboosh, Sanjeet Grewal, Kaisorn Chaichana, Robert Wharen, Hugo Guerrero-Cazares, Alfredo Quinones-Hinojosa, Nilufer Ertekin-Taner
Summary: This study comprehensively characterizes the transcriptomes of human microglia and explores the effects of age, sex, and APOE. The study identifies microglial co-expression network modules associated with age, sex, and APOE-epsilon 4 that are enriched for lipid and carbohydrate metabolism genes. These modules and disease-associated microglial clusters contain known neurodegenerative disease genes.
Article
Neurosciences
Emily S. Norton, Lauren A. Whaley, Maria Jose Ulloa-Navas, Patricia Garcia-Tarraga, Kayleah M. Meneses, Montserrat Lara-Velazquez, Natanael Zarco, Anna Carrano, Alfredo Quinones-Hinojosa, Jose Manuel Garcia-Verdugo, Hugo Guerrero-Cazares
Summary: This study demonstrates for the first time that glioblastomas (GBMs) proximal to the lateral ventricles (LVs) disrupt the ependymal cell barrier, leading to compromised ependymal cell health and increased interaction between cerebrospinal fluid (CSF) and the tumor. These findings suggest that ependymal cell health and CSF-contained molecules could be potential therapeutic targets in the treatment of GBM.
FLUIDS AND BARRIERS OF THE CNS
(2022)
Article
Cell & Tissue Engineering
Colleen M. Miller, Elizabeth Ann L. Enninga, Skylar A. Rizzo, Jordan Phillipps, Hugo Guerrero-Cazares, Christopher C. Destephano, Timothy E. Peterson, Paul G. Stalboerger, Atta Behfar, Zaraq Khan
Summary: The study found that a platelet-derived purified exosome product (PEP) can be absorbed by various types of endometrial cells. PEP treatment can increase cell proliferation and wound healing capacity.
REGENERATIVE MEDICINE
(2022)
Article
Cell Biology
Beatriz Fernandez-Gil, Andrea Otamendi-Lopez, Alexandra Bechtle, Carla A. Vazquez-Ramos, Neda Qosja, Paola Suarez-Meade, Rachel Sarabia-Estrada, Mark E. Jentoft, Hugo Guerrero-Cazares, Germaine Escames, Paula Schiapparelli, Alfredo Quinones-Hinojosa
Summary: This study reveals that melatonin inhibits the proliferation and survival of GBM tumor initiating cells by modulating intracellular pH and inducing cellular death. These findings provide new insights into the oncostatic effect of melatonin on GBM and may contribute to the development of new therapeutic strategies.
Editorial Material
Biochemistry & Molecular Biology
Amit Sharma, Hugo Guerrero-Cazares, Jarek Maciaczyk
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Oluwaseun O. Akinduro, Paola Suarez-Meade, McKinley Roberts, Stephany Y. Tzeng, Rachel Sarabia-Estrada, Paula Schiapparelli, Emily S. Norton, Ziya L. Gokaslan, Panos Z. Anastasiadis, Hugo Guerrero-Cazares, Jordan J. Green, Alfredo Quinones-Hinojosa
Summary: This study demonstrates that verteporfin-mediated inhibition of YAP can reduce tumorigenicity in lung and breast spinal metastatic cancer cells, offering promising results for potential translation to human clinical trials.
JOURNAL OF NEUROSURGERY-SPINE
(2023)
Review
Biochemistry & Molecular Biology
Marissa N. Russo, Lauren A. Whaley, Emily S. Norton, Natanael Zarco, Hugo Guerrero-Cazares
Summary: Glioblastoma (GBM) is the most malignant form of gliomas and the most common and deadly primary brain tumor in adults. The tumor microenvironment (TME), including the perivascular niche, the subventricular zone neurogenic niche, and the immune microenvironment, plays an important role in GBM biology and tumor maintenance. Extracellular vesicles, such as exosomes and microvesicles, facilitate communication between GBM cells and TME cells, carrying cancerous and non-cancerous cargo. This review highlights the potential of extracellular vesicles as diagnostic and targetable vehicles for GBM.
MOLECULAR ASPECTS OF MEDICINE
(2023)
Article
Oncology
Luisina B. Ripari, Emily S. Norton, Raquel Bodoque-Villar, Stephanie Jeanneret, Montserrat Lara-Velazquez, Anna Carrano, Natanael Zarco, Carla A. Vazquez-Ramos, Alfredo Quinones-Hinojosa, Carlos De La Rosa-Prieto, Hugo Guerrero-Cazares
Summary: A novel rodent model was used to analyze the bidirectional signaling between GBM tumors and cells within the subventricular zone (SVZ). The study found that GBM tumors located close to the lateral ventricles show increased tumor growth, decreased median survival, and negatively influence the neurogenic and cell division process of the SVZ in adult mice. This model will be invaluable for further investigation into the interactions between GBM and the neurogenic cell populations of the SVZ.
FRONTIERS IN ONCOLOGY
(2021)