Article
Chemistry, Physical
T. B. Thompson, G. Meisl, T. P. J. Knowles, A. Goriely
Summary: The deposition of pathological protein aggregates in the brain plays a central role in cognitive decline and structural damage associated with neurodegenerative diseases. In vivo clearance mechanisms are believed to play an essential role in limiting the formation of aggregates and preventing disease emergence. Small variations in the efficiency of the clearance process can lead to a sudden shift from a healthy to a disease state.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Pharmacology & Pharmacy
Bhushan Munjal, Sajal M. Patel, Raj Suryanarayanan
Summary: This study evaluated the stabilization potential of arginine hydrochloride in frozen solutions and found that it had a certain inhibitory effect on protein aggregation. The crystallization of mannitol facilitated the crystallization of arginine hydrochloride, leading to the loss of its stabilizing effect in the presence of mannitol. However, the use of alternate arginine salts prevented this issue, maintaining the amorphous state of arginine and stabilizing the protein.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Medicine, Research & Experimental
Cory Wilson, Tinne C. J. Mertens, Pooja Shivshankar, Weizen Bi, Scott D. Collum, Nancy Wareing, Junsuk Ko, Tingting Weng, Ram P. Naikawadi, Paul J. Wolters, Pascal Maire, Soma S. K. Jyothula, Rajarajan A. Thandavarayan, Dewei Ren, Nathan D. Elrod, Eric J. Wagner, Howard J. Huang, Burton F. Dickey, Heide L. Ford, Harry Karmouty-Quintana
Summary: The transcription factor SIX1 is found to play a crucial role in pulmonary fibrosis, and its overexpression may contribute to the development of fibrosis. SIX1 binds to the MIF promoter, regulating the expression of MIF and participating in the pathogenesis of lung fibrosis.
Article
Developmental Biology
Roxan A. Stephenson, Jonathon M. Thomalla, Lili Chen, Petra Kolkhof, Roger P. White, Mathias Beller, Michael A. Welte
Summary: The study reveals how histones H2B, H2A, and H2Av accumulate on lipid droplets in Drosophila embryos and the crucial role of lipid droplets in this process. Lipid droplets in nurse cells facilitate the transport of histones to the oocyte, ensuring the storage of histones for cellular function and development.
Article
Biochemistry & Molecular Biology
Wenjuan Zhang, Bo Huang, Limo Gao, Cao Huang
Summary: Research has shown that a UBQLN2 mutation associated with ALS/FTD disrupts proteasome integrity in rats, leading to accumulation of proteasome subunits and impaired proteasome function. As the disease progresses and with increasing age, proteasome subunits are translocated to the nucleus, suggesting that defective proteasome function may result from mislocalization of subunits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Hyun-Jung Kim, Hye-Rim Shin, Heein Yoon, Min-Sang Park, Byung-Gyu Kim, Jae- Moon, Woo-Jin Kim, Seung Gwa Park, Ki-Tae Kim, Ha-Neui Kim, Je-Yong Choi, Hyun-Mo Ryoo
Summary: PADI2 deficiency leads to bone loss and a disorder called cleidocranial dysplasia, which includes delayed skull development and clavicular hypoplasia. Mechanistically, PADI2 stabilizes RUNX2 protein, playing a role in bone formation and osteoblast differentiation.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Jennifer Cable, Eilika Weber-Ban, Tim Clausen, Kylie J. Walters, Michal Sharon, Daniel J. Finley, Yangnan Gu, John Hanna, Yue Feng, Sascha Martens, Anne Simonsen, Malene Hansen, Hong Zhang, Jonathan M. Goodwin, Alessio Reggio, Chunmei Chang, Liang Ge, Brenda A. Schulman, Raymond J. Deshaies, Ivan Dikic, J. Wade Harper, Ingrid E. Wertz, Nicolas H. Thoma, Mikolaj Slabicki, Judith Frydman, Ursula Jakob, Della C. David, Eric J. Bennett, Carolyn R. Bertozzi, Richa Sardana, Vinay V. Eapen, Serena Carra
Summary: Targeted protein degradation is essential for cellular function and development. This process involves tightly regulated protein degradation pathways to eliminate misfolded and aggregated proteins, adjust protein levels during cellular differentiation, and selectively eliminate target proteins. Understanding these pathways can provide insights into disease pathology and the development of novel therapeutics.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Plant Sciences
Guoren He, Ren Zhang, Shenghang Jiang, Huanhuan Wang, Feng Ming
Summary: In this study, a key R2R3-MYB transcription factor, RcMYB1, was found to play a central role in rose anthocyanin biosynthesis. Overexpression of RcMYB1 significantly promoted anthocyanin accumulation in both white rose petals and tobacco leaves. Two MBW complexes associated with anthocyanin accumulation were also identified.
HORTICULTURE RESEARCH
(2023)
Review
Cell Biology
Mingxia Bi, Xixun Du, Qian Jiao, Xi Chen, Hong Jiang
Summary: The proteasome is crucial for protein degradation, and dysregulation of its function can lead to diseases. Activating the proteasome may help prevent neurodegeneration in diseases like Parkinson's. Various strategies targeting different levels can maintain proteasome homeostasis and may offer promising approaches for clinical interventions in diseases involving protein aggregation.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Anusha R. Pallapati, Sri D. Sirigiri, Swati Jain, Vamsi Ratnala, Ipsita Roy
Summary: This study investigated the role of K245 in the catalytic cycle of yeast Gpd1, revealing its importance for the conformational stability and functional adaptation of Gpdl. The K245A mutant showed lower catalytic activity and a relatively unstable, aggregation- and degradation-prone conformation. In silico studies identified an aggregation hotspot around K245.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Daniel Kalb, Huy D. Vo, Samantha Adikari, Elizabeth Hong-Geller, Brian Munsky, James Werner
Summary: IL-1 beta and TNF-alpha are canonical immune response mediators that play key regulatory roles in various inflammatory responses. Using single-cell mRNA analysis, we found that U0126 and MG132 can successfully block the expression of these genes, indicating the potential for targeted drug interventions in immune responses.
SCIENTIFIC REPORTS
(2021)
Article
Clinical Neurology
Katherine R. Croce, Ai Yamamoto
Summary: Many adult-onset neurodegenerative diseases are characterized by the deposition and accumulation of misfolded proteins, but their exact role in pathogenesis remains unclear. Recent research identified a key protein, Alfy/Wdfy3, involved in the specific turnover of aggregated proteins in the adult brain, which accelerated the accumulation of mutant huntingtin protein in a mouse model of Huntington's disease. Although motor dysfunction was accelerated, it did not lead to an increase in cell loss, suggesting that protein aggregates modify circuit dysfunction rather than drive degeneration itself.
MOVEMENT DISORDERS
(2021)
Article
Medicine, Research & Experimental
Jinghan Li, Jayesh Sonje, Raj Suryanarayanan
Summary: The phase behavior of poloxamer 188 in aqueous solutions was studied using DSC and synchrotron X-ray diffractometry, showing solute crystallization during both freezing and thawing. Sucrose and trehalose inhibited P188 crystallization during freeze-thawing. LDH served as a model protein to evaluate the stabilizing effect of P188, showing that higher concentrations of P188 stabilized the protein while lower concentrations led to protein aggregation.
MOLECULAR PHARMACEUTICS
(2023)
Review
Pharmacology & Pharmacy
Boya Chen, Haiying Zhu, Bo Yang, Ji Cao
Summary: Immunoproteasome is a variant of proteasome optimized for producing antigenic peptides with higher binding affinity and plays a dual role in different types of cancer. It is involved in antigen presentation and degradation of proteins, including tumor suppressor proteins, contributing to tumor progression. Targeting immunoproteasome could be a potential therapeutic intervention in cancer treatment and understanding its role is crucial for cancer prevention and treatment.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Cell Biology
Xuhua Duan, Hao Li, Manzhou Wang, Shuguang Ju, Fengyao Li, Pengfei Chen, Huibin Lu, Xinwei Han, Jianzhuang Ren
Summary: This study reveals that the overexpression of PSMC2 in HCC tissues is significantly associated with tumor infiltrate and stage. Knockdown of PSMC2 impairs proliferation, migration, and promotes apoptosis in HCC cells. Furthermore, the study demonstrates a direct interaction between PSMC2 and ITGA6, with PSMC2 knockdown exacerbating the inhibitory effects of ITGA6 depletion on HCC.
CELL DEATH DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Masanori Chiba, Hiroyoshi Ariga, Hiroshi Maita
CHEMICAL BIOLOGY & DRUG DESIGN
(2016)
Article
Biochemistry & Molecular Biology
Kazuko Takahashi-Niki, Izumi Kato-Ose, Hiroaki Murata, Hiroshi Maita, Sanae M. M. Iguchi-Ariga, Hiroyoshi Ariga
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Biochemical Research Methods
Hiroshi Maita, Kenji Tomita, Hiroyoshi Ariga
ANALYTICAL BIOCHEMISTRY
(2014)
Article
Neurosciences
Mariko Takano, Erika Tashiro, Akira Kitamura, Hiroshi Maita, Sanae M. M. Iguchi-Ariga, Masataka Kinjo, Hiroyoshi Ariga
Article
Biochemistry & Molecular Biology
Shizuma Ishikawa, Takahiro Taira, Takeshi Niki, Kazuko Takahashi-Niki, Chinatsu Maita, Hiroshi Maita, Hiroyoshi Ariga, Sanae M. M. Iguchi-Ariga
JOURNAL OF BIOLOGICAL CHEMISTRY
(2009)
Article
Biochemistry & Molecular Biology
Makoto Miyazawa, Erika Tashiro, Hirotake Kitaura, Hiroshi Maita, Hiroo Suto, Sanae M. M. Iguchi-Ariga, Hiroyoshi Ariga
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Izumi Kato, Hiroshi Maita, Kazuko Takahashi-Niki, Yoshiro Saito, Noriko Noguchi, Sanae M. M. Iguchi-Ariga, Hiroyoshi Ariga
MOLECULAR AND CELLULAR BIOLOGY
(2013)
Article
Neurosciences
Yoshihisa Kitamura, Shotaro Watanabe, Masanobu Taguchi, Kentaro Takagi, Takuya Kawata, Kazuko Takahashi-Niki, Hiroyuki Yasui, Hiroshi Maita, Sanae M. M. Iguchi-Ariga, Hiroyoshi Ariga
MOLECULAR NEURODEGENERATION
(2011)
Review
Cell Biology
Hiroyoshi Ariga, Kazuko Takahashi-Niki, Izumi Kato, Hiroshi Maita, Takeshi Niki, Sanae M. M. Iguchi-Ariga
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2013)
Article
Multidisciplinary Sciences
Chinatsu Maita, Hiroshi Maita, Sanae M. M. Iguchi-Ariga, Hiroyoshi Ariga
Article
Multidisciplinary Sciences
Rabiah M. Mayas, Hiroshi Maita, Daniel R. Semlow, Jonathan P. Staley
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2010)
Review
Cell Biology
Hiroshi Maita, Shinichi Nakagawa
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2020)
Article
Cell Biology
Osamu Takahashi, Mayuko Tanahashi, Saori Yokoi, Mari Kaneko, Kaori Yanaka, Shinichi Nakagawa, Hiroshi Maita
Summary: This study focused on a poorly characterized protein UGS148 highly expressed in a specialized cell type. The protein was found to associate with mitochondrial ATPase and showed overlapping signals with ER in tanycytes. Mutant mice lacking UGS148 did not exhibit overt phenotypes, highlighting the importance of experimental validation of probe specificity in RNA localization studies.
Article
Pharmacology & Pharmacy
Kenji Tomita, Shinichi Nakagawa, Hiroyoshi Ariga, Hiroshi Maita
Summary: Intron recognition by the spliceosome depends on conserved intronic sequences and mutations in the spliceosome components can affect splicing fidelity. We identified a compound, BAY61-3606, from a small-compound library that enhanced splicing at mutated splice sites. This compound changed the cellular small nuclear ribonucleoprotein composition, indicating its potential for modulation of splicing fidelity.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2023)