Article
Cell Biology
Joseph Atherton, Melissa Stouffer, Fiona Francis, Carolyn A. Moores
Summary: We used cryo-electron tomography to investigate the subcellular architecture of mouse hippocampal neuron growth cones. Our findings revealed the penetration of hexagonal arrays of actin bundles and the modulation of actin bundles by altering individual F-actin helical structures. Microtubules with different end structures and lattice defects were observed in the growth cone. Absence of the cytoskeleton regulator doublecortin had no major effect on growth cone organization but highlighted the importance of microtubule integrity during growth cone migration.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Varun Venkataramani, Yvonne Yang, Marc Cicero Schubert, Ekin Reyhan, Svenja Kristin Tetzlaff, Niklas Wissmann, Michael Botz, Stella Judith Soyka, Carlo Antonio Beretta, Rangel Lyubomirov Pramatarov, Laura Fankhauser, Luciano Garofano, Alexander Freudenberg, Julia Wagner, Dimitar Ivanov Tanev, Miriam Ratliff, Ruifan Xie, Tobias Kessler, Dirk C. Hoffmann, Ling Hai, Yvette Doerflinger, Simone Hoppe, Yahaya A. Yabo, Anna Golebiewska, Simone P. Niclou, Felix Sahm, Anna Lasorella, Martin Slowik, Leif Doering, Antonio Iavarone, Wolfgang Wick, Thomas Kuner, Frank Winkler
Summary: A subpopulation of glioblastoma cells lacking connections with other tumor cells and astrocytes but receiving synaptic input from neurons drives whole-brain colonization. These cells resemble neuronal and neural-progenitor-like states and invade the brain using mechanisms similar to neuronal migration. Neuronal activity induces calcium signals, leading to the formation of tumor microtubes and increased invasion speed. This study reveals how neuronal mechanisms govern glioblastoma cell invasion and explains the close interlink between tumor dissemination and cellular heterogeneity.
Article
Multidisciplinary Sciences
Dong Jin Mun, Bon Seong Goo, Bo Kyoung Suh, Ji-Ho Hong, Youngsik Woo, Soo Jeong Kim, Seunghyun Kim, Su Been Lee, Yubin Won, Jin Yeong Yoo, Eunbyul Cho, Eun Jin Jang, Truong Thi My Nhung, Hong Minh Triet, Hongyul An, Haeryun Lee, Minh Dang Nguyen, Seung-Yeol Park, Seung Tae Baek, Sang Ki Park
Summary: In this study, the Gcap14-Ndel1 complex is identified as a fundamental regulator of cytoskeletal remodeling during neurodevelopment, including neuronal process elongation and migration. Deficiency of Gcap14 leads to impaired cortical lamination and defective neuronal migration. The interaction between Gcap14 and Ndel1 effectively corrects the downregulation of microtubule dynamics and the defects in neuronal migration caused by Gcap14 deficiency.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Neurosciences
Camille Cuveillier, Benoit Boulan, Charlotte Ravanello, Eric Denarier, Jean-Christophe Deloulme, Sylvie Gory-Faure, Christian Delphin, Christophe Bosc, Isabelle Arnal, Annie Andrieux
Summary: MAPs play crucial roles in the development and function of the central nervous system by influencing microtubules and actin cytoskeletons, participating in signaling pathways, and contributing to molecular actions.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Cell Biology
Abhishek Ankur Balmik, Shweta Kishor Sonawane, Subashchandrabose Chinnathambi
Summary: The study shows that HDAC6's ZnF UBP domain can modulate Tau phosphorylation, actin cytoskeleton organization, neurite extension, and ApoE nuclear localization. Additionally, it plays a role in increasing tubulin localization in the microtubule organizing center. The ZnF domain of HDAC6 is involved in various regulatory functions in neurodegenerative diseases beyond its classical role in aggresome formation.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Neurosciences
Hemalatha Muralidharan, Shrobona Guha, Kiran Madugula, Ankita Patil, Sarah A. Bennison, Xiaohuan Sun, Kazuhito Toyo-oka, Peter W. Baas
Summary: This study reveals the essential role of KIFC1 in ensuring the accuracy of neuronal migration trajectory. By crosslinking microtubules into a nonsliding mode, KIFC1 enables dynein-driven forces to achieve sufficient traction and thus allows neurons to migrate along their appropriate path.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Cell Biology
Lena Hoffmann, Marcel S. Waclawczyk, Stephan Tang, Eva-Maria Hanschmann, Manuela Gellert, Marco B. Rust, Carsten Culmsee
Summary: This study demonstrates that the actin-regulating protein cofilin1 plays a crucial role in oxidative neuronal death by affecting mitochondrial function and oxidation. Additionally, cofilin1 can influence cellular metabolic pathways and resistance to oxidative stress.
CELL DEATH & DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Jayne Aiken, Erika L. F. Holzbaur
Summary: The development and proper function of the brain require the intricate formation of neuronal circuitry through synaptic connections between neurons, which must be maintained over a lifetime. Accurate trafficking of presynaptic and postsynaptic components, as well as readily accessible energy stores from localized mitochondria, are crucial for synapse-specific delivery and plasticity. Recent research has shed light on the importance of local regulation of cytoskeletal dynamics and organelle trafficking in establishing and maintaining neuronal circuitry.
Article
Multidisciplinary Sciences
Max Laurens Bastiaan Gronloh, Janine Johanna Geertruida Arts, Eike Karin Mahlandt, Martijn A. Nolte, Joachim Goedhart, Jaap Diederik van Buul
Summary: During inflammation, primary neutrophil adhesion to the endothelium triggers the formation of transmigration hotspots, allowing secondary neutrophils to cross the endothelium more efficiently. This process is mediated by the activation of JNK1 and MARCKSL1, which induces the formation of filopodia and increases neutrophil adhesion.
Article
Biochemistry & Molecular Biology
Alessandro Bortolami, Wei Yu, Elena Forzisi, Koray Ercan, Ritik Kadakia, Madhuvika Murugan, Denise Fedele, Irving Estevez, Detlev Boison, Mladen-Roko Rasin, Federico Sesti
Summary: The study investigates the role of voltage-gated potassium channels in neocortical development. A genetic mutation in these channels can lead to severe neuromorphological abnormalities, but can be rescued through pharmacological intervention. This research reveals the importance of developmental channelopathies in the etiology of certain brain disorders.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biology
Campbell D. Lawson, Samantha Peel, Asier Jayo, Adam Corrigan, Preeti Iyer, Mabel Baxter Dalrymple, Richard J. Marsh, Susan Cox, Isabel Van Audenhove, Jan Gettemans, Maddy Parsons
Summary: Fascin is an important regulator of F-actin bundling in tumor cells. It has been found that Fascin bundles F-actin within the nucleus, supporting chromatin organization and efficient DNA damage response. Increased nuclear accumulation of Fascin leads to sustained nuclear F-actin bundling, resulting in reduced invasion, viability, and apoptosis of cancer cells.
Article
Cell Biology
Yuzhen Wang, Yingchun Wang, Wen Liu, Lu Ding, Xiaodi Zhang, Bo Wang, Zheng Tong, Xuetian Yue, Chunyang Li, Liyun Xu, Zhuanchang Wu, Xiaohong Liang, Chunhong Ma, Lifen Gao
Summary: The study found that T-cell immunoglobulin and mucin domain-containing molecule 4 (TIM-4) promotes the growth and proliferation of lung cancer cells by enhancing mitochondrial fusion and oxidative phosphorylation pathway.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Jianing Song, Ronald A. Merrill, Andrew Y. Usachev, Stefan Strack
Summary: The E3 ubiquitin ligase adaptor KLHL15 plays a critical role in maintaining the proteostasis of neuronal microtubule-associated proteins, which are essential for early brain development. Interactions with KLHL15 lead to ubiquitination and proteasomal degradation of key proteins like DCX and doublecortin-like kinases, influencing dendritic complexity in hippocampal neurons and potentially impacting the development of the mammalian nervous system.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Sylvie Gory-Faure, Rebecca Powell, Julie Jonckheere, Fabien Lante, Eric Denarier, Leticia Peris, Chi Hung Nguyen, Alain Buisson, Laurence Lafanechere, Annie Andrieux
Summary: The search for effective treatments for neuropsychiatric disorders is ongoing, with modulation of LIMK activity being a potential new therapeutic strategy. Experimental results show that long-term treatment with Pyr1 can improve synaptic plasticity defects and behavioral disorders in animal models.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Virginia Andrade, Jian Bai, Neetu Gupta-Rossi, Ana Joaquina Jimenez, Cedric Delevoye, Christophe Lamaze, Arnaud Echard
Summary: Caveolae play a crucial role in promoting cytokinetic abscission by buffering membrane tension and limiting contractibility at the intercellular bridge.
Article
Biochemistry & Molecular Biology
Manami Toriyama, Michinori Toriyama, John B. Wallingford, Richard H. Finnell
Article
Biology
Kanako Kumamoto, Tokuichi Iguchi, Ryuichi Ishida, Takuya Uemura, Makoto Sato, Shinji Hirotsune
Article
Multidisciplinary Sciences
Kouki Abe, Hiroko Katsuno, Michinori Toriyama, Kentarou Baba, Tomoyuki Mori, Toshio Hakoshima, Yonehiro Kanemura, Rikiya Watanabe, Naoyuki Inagaki
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Kensuke Matsumura, Masayuki Baba, Kazuki Nagayasu, Kana Yamamoto, Momoka Kondo, Kohei Kitagawa, Tomoya Takemoto, Kaoru Seiriki, Atsushi Kasai, Yukio Ago, Atsuko Hayata-Takano, Norihito Shintani, Toshihiko Kuriu, Tokuichi Iguchi, Makoto Sato, Kazuhiro Takuma, Ryota Hashimoto, Hitoshi Hashimoto, Takanobu Nakazawa
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Review
Genetics & Heredity
Noriaki Sasai, Michinori Toriyama, Toru Kondo
FRONTIERS IN GENETICS
(2019)
Article
Multidisciplinary Sciences
Kensuke Matsumura, Kaoru Seiriki, Shota Okada, Masashi Nagase, Shinya Ayabe, Ikuko Yamada, Tamio Furuse, Hirotoshi Shibuya, Yuka Yasuda, Hidenaga Yamamori, Michiko Fujimoto, Kazuki Nagayasu, Kana Yamamoto, Kohei Kitagawa, Hiroki Miura, Nanaka Gotoda-Nishimura, Hisato Igarashi, Misuzu Hayashida, Masayuki, Momoka Kondo, Shigeru Hasebe, Kosei Ueshima, Atsushi Kasai, Yukio Ago, Atsuko Hayata-Takano, Norihito Shintani, Tokuichi Iguchi, Makoto Sato, Shun Yamaguchi, Masaru Tamura, Shigeharu Wakana, Atsushi Yoshiki, Ayako M. Watabe, Hideyuki Okano, Kazuhiro Takuma, Ryota Hashimoto, Hitoshi Hashimoto, Takanobu Nakazawa
NATURE COMMUNICATIONS
(2020)
Article
Dermatology
Defri Rizaldy, Manami Toriyama, Hiroko Kato, Runa Fukui, Fumitaka Fujita, Motoki Nakamura, Fumihiro Okada, Akimichi Morita, Ken J. Ishii
Summary: This study revealed an increase in ciliated cells in skin samples from patients with atopic dermatitis (AD) and psoriasis, with the formation of primary cilia being modulated by stimulation of keratinocytes using Th2 and Th17 cytokines. This modulation was associated with an increase in JNK phosphorylation, which may impair keratinocyte differentiation under inflammatory stress conditions in both AD and psoriasis patients.
EXPERIMENTAL DERMATOLOGY
(2021)
Review
Cell Biology
Manami Toriyama, Ken J. Ishii
Summary: The skin, as the largest organ of the human body, acts as a physical and immunological barrier against pathogen infection. There is still little known about which cells in human skin have primary cilia and how they change in response to immune reactions or disease. Several studies have described mechanisms of cilia regulation by immune reactions and the physiological relevance of cilia regulating proliferation and differentiation of stroma cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Tokuichi Iguchi, Yuichiro Oka, Misato Yasumura, Minoru Omi, Kazuki Kuroda, Hideshi Yagi, Min-Jue Xie, Manabu Taniguchi, Martin Bastmeyer, Makoto Sato
Summary: The expression and repulsive activities of EphA7 and EfnA5 in the cortex and basilar pons are crucial for segregating collateral extensions and directing corticopontine projections in mice.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Neurosciences
Yuichiro Oka, Miyuki Doi, Manabu Taniguchi, Sheena Y. X. Tiong, Hisanori Akiyama, Takuto Yamamoto, Tokuichi Iguchi, Makoto Sato
Summary: The study found that dual-projection neurons in the mouse neocortex make up a significant portion of the association projections to the primary motor cortex, supporting a directed guidance mechanism in long-range corticocortical circuit formation. The developmental processes of these association projections, especially in the early stages before reaching the target areas, remain unknown.
Article
Biology
Mai Quynh Nguyen, Manabu Taniguchi, Misato Yasumura, Tokuichi Iguchi, Makoto Sato
Summary: Actin-based protrusions called cytonemes, which play important roles in cell communication, are formed through a molecular mechanism that involves the LAR receptor protein. This study shows that LAR dimerization triggers the formation of longer and more branched cytoneme-like protrusions, emphasizing the significance of LAR and its dimerization in cell signaling.
Article
Cell Biology
Yusuke Nojima, Manami Toriyama, Kenji Tago, Norikazu Mizuno, Kazuhiro Morishita, Hiroshi Itoh
Summary: This study investigated the expression profiles of two aGPCRs in the mouse brain and found that their fragments are expressed in different brain regions at different developmental stages. The study demonstrated the formation of a functional complex through non-covalent interaction between these fragments, which is involved in neuronal signal transduction.
Article
Biochemistry & Molecular Biology
Ria Fajarwati Kastian, Kentarou Baba, Napol Kaewkascholkul, Hisashi Sasaki, Rikiya Watanabe, Michinori Toriyama, Naoyuki Inagaki
Summary: Axon pathfinding is crucial for neuronal network formation. Shootin1a acts as a mechanical linker molecule involved in axon outgrowth and guidance. Concentration gradients of axon guidance molecule netrin-1 induce asymmetric localization of phosphorylated shootin1a, which promotes local actin-adhesion coupling and directional forces for growth cone turning. Protein phosphatase-1 (PP1) is found to dephosphorylate shootin1a in the growth cone, regulating netrin-1-induced axon guidance.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Min-Jue Xie, Yasuyuki Ishikawa, Hideshi Yagi, Tokuichi Iguchi, Yuichiro Oka, Kazuki Kuroda, Keiko Iwata, Hiroshi Kiyonari, Shinji Matsuda, Hideo Matsuzaki, Michisuke Yuzaki, Yugo Fukazawa, Makoto Sato
SCIENTIFIC REPORTS
(2019)
