Article
Cell Biology
Jan-Gowth Chang, Ni Tien, Yi-Chih Chang, Meng-Liang Lin, Shih-Shun Chen
Summary: This study reveals a novel molecular mechanism of the pro-apoptotic activity controlled by oxidative stress to modulate ER-mitochondrial bioenergetic metabolism, as well as suggests the disruption of CDK1-cyclin B1-mediated BCL-2/BCL-x(L) oncogenic activity by triggering oxidative stress-induced PI3K-Akt-GTP-Rac1 inactivation as a therapeutic approach for NPC.
Article
Cell Biology
Chenxi Zhou, Hayden A. Homer
Summary: During fertilization in mice, Cdk1 activity, cell division, and pronuclear formation go through two pauses, with the first pause triggering cell division and the second pause triggering pronuclear formation. Both pauses require the inhibitory Cdk1 kinase Wee1B, but only the first pause involves the degradation of cyclin B1.
Article
Oncology
Shigeo Ohba, Yongjian Tang, Tor-Christian Aase Johannessen, Joydeep Mukherjee
Summary: PKM2 interacts with and enhances the activation of the Cdk1-cyclin B complex, thereby directly controlling the progression of mitosis and the growth of brain tumor cells.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Martina Barbiero, Luca Cirillo, Sapthaswaran Veerapathiran, Catherine Coates, Camilla Ruffilli, Jonathon Pines
Summary: This study uses fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy to investigate the dynamics of the cell cycle machinery. The results show the existence of an unbound pool of Cyclin B1 in living cells and demonstrate that the affinity of Cyclin B1 for Cdk1 increases during the cell cycle, indicating regulated assembly of the complex.
Article
Virology
Xifeng Hu, Zheng Chen, Xiangdong Wu, Zhen Ding, Yu Huang, Qiuling Fu, Zhen Chen, Huansheng Wu
Summary: Infectious bursal disease virus (IBDV) poses a great economic threat to the global poultry farming industry. CDK1-cyclin B1 accumulates in the cytoplasm and phosphorylates VP1 on serine 7, which is necessary for viral replication. Inhibiting CDK1 or silencing CDK1-cyclin B1 decreases IBDV replication.
JOURNAL OF VIROLOGY
(2023)
Article
Pharmacology & Pharmacy
M. Suleimenov, S. Bekbayev, M. Ten, N. Suleimenova, M. Tlegenova, A. Nurmagambetova, S. Kauanova, I. Vorobjev
Summary: Microtubule-targeting drugs taxanes and vinca alkaloids have been widely used in chemotherapy due to their ability to block mitotic progression. This study determined the maximal and minimal doses of mitotic inhibitors that cells can tolerate and showed that cell death during mitotic arrest and after slippage proceed via mitochondria-dependent apoptosis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Jiexiang Zhao, Ping Lu, Cong Wan, Yaping Huang, Manman Cui, Xinyan Yang, Yuqiong Hu, Yi Zheng, Ji Dong, Mei Wang, Shu Zhang, Zhaoting Liu, Shuhui Bian, Xiaoman Wang, Rui Wang, Shaofang Ren, Dazhuang Wang, Zhaokai Yao, Gang Chang, Fuchou Tang, Xiao-Yang Zhao
Summary: Through single-cell sequencing, the study investigates the transcriptome landscapes of mouse male germ cells throughout development and identifies critical regulators for prenatal cell-fate determination.
NATURE COMMUNICATIONS
(2021)
Article
Virology
Yu-Jin Kim, Haydar Witwit, Beatrice Cubitt, Juan C. de la Torre
Summary: This study reveals that Bcl-2 inhibitors mediate cell cycle arrest at the G0/G1 phase, rather than their proapoptotic activity, to play a critical role in blocking mammarenavirus multiplication. Additionally, the Bcl-2 inhibitor ABT-737 exhibited moderate efficacy against mammarenaviruses in vivo, showcasing broad-spectrum antiviral activities against various species and SARS-CoV-2. These results suggest that Bcl-2 inhibitors might be repositioned as effective broad-spectrum antivirals.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jason M. Keaton, Benjamin G. Workman, Linfeng Xie, James R. Paulson
Summary: We demonstrate that specific inactivation of the protein kinase Cdk1/cyclin B triggers exit from mitosis in budding yeast, and that inactivation of Cdk1 is both necessary and sufficient to initiate the transition from mitosis to G1 phase. Using this system, we show that protein phosphatase 1 is required for mitotic exit and reestablishment of interphase following Cdk1 inactivation, and propose the use of this system to test the requirement of other protein phosphatases.
CHROMOSOME RESEARCH
(2023)
Article
Oncology
Shu-Yuan Hu, Jin-Xian Qian, Shao-Ying Yang, Lisa Andriani, Li Liao, Ling Deng, Min-Ying Huang, Yin-Ling Zhang, Fang-Lin Zhang, Zhi-Min Shao, Da-Qiang Li
Summary: It was found that MORC2 is a newly identified regulator associated with cancer drug resistance. MORC2 activates the spindle assembly checkpoint, promotes mitotic progression, and affects the sensitivity of cancer cells to paclitaxel and vincristine. These findings provide a new clue for combined treatment strategy targeting MORC2 in combination with MTAs against human cancer.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Delgado, Randall R. Rainwater, Billie Heflin, Alicja Urbaniak, Kaitlynn Butler, Mari Davidson, Reine M. Protacio, Giulia Baldini, Andrea Edwards, Megan R. Reed, Kevin D. Raney, Timothy C. Chambers
Summary: This study found that microtubule depolymerization induces different cell death pathways depending on the specific phase of the cell cycle. Cell death in M phase is associated with mitochondrial-mediated apoptosis, while cell death in G1 phase is associated with loss of mitochondrial transmembrane potential, parylation, nuclear translocation of apoptosis-inducing factor and endonuclease G, and supra-nucleosomal DNA fragmentation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Minjun Meng, Qiaolu Yang, Zhong Ouyang, Qingmo Yang, Xinyi Wu, Yufan Huang, Yonghui Su, Shuanglong Chen, Wenlin Chen
Summary: This study found that Ampelopsin (AMP), extracted from the root of Ampelopsis grossedentata, could significantly inhibit the growth of breast cancer MDA-MB-231 cells by inactivating the PI3K/AKT/mTOR pathway, leading to cell cycle arrest at G2/M phase. The results shed light on the potential therapeutic role of AMP in breast cancer treatment.
ACTA PHARMACEUTICA
(2023)
Article
Biochemistry & Molecular Biology
Fangfang Duan, Ying Gao, Xiaogang Peng, Xianggao Meng, Jinling Chang, Yutian Gan, Qianxi Ouyang, Hanli Ruan
Summary: Fourteen new chaetoglobosins were isolated from an endophytic fungus, with one compound displaying significant cytotoxic effects against seven human cancer cell lines by inducing cell cycle arrest and apoptosis.
BIOORGANIC CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
W. Douglas Fairlie, Erinna F. Lee
Summary: The deregulation of apoptosis plays a crucial role in tumorigenesis, and drugs targeting the prosurvival members of the BCL-2 protein family called BH3-mimetics have shown success in treating hematological malignancies. However, their potential in solid cancer therapy has not been fully realized due to the lack of effective combination strategies to target multiple pro-survival proteins. Exciting new developments in the field have the potential to unleash the full power of BH3-mimetic therapy in treating currently difficult-to-treat solid malignancies.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)