Article
Medicine, Research & Experimental
Shupeng Hu, Qiangzhong Pi, Xiudan Xu, Jianghong Yan, Yongzheng Guo, Wanying Tan, An He, Zhe Cheng, Suxin Luo, Yong Xia
Summary: This study investigates the role of eNOS in vascular dysfunction induced by sepsis. Increased phosphorylation of eNOS in early sepsis leads to decreased vasodilation, while decreased levels of eNOS in late sepsis result in diminished nitric oxide-dependent relaxation responses.
Review
Pharmacology & Pharmacy
Zhengyao Cai, Suxin Yuan, Yi Zhong, Li Deng, Jiafu Li, Xiaoqiu Tan, Jian Feng
Summary: Diabetes eventually leads to chronic vascular complications, with TGR5 potentially serving as a novel therapeutic target for diabetes-associated endothelial dysfunction.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Michael G. Stencel, Mark VerMeer, Jennifer Giles, Quang-Kim Tran
Summary: Calmodulin (CaM) is a crucial protein for multiple functions of vascular smooth muscle cells (VSMCs) and its expression and interactions with target proteins in VSMCs can be regulated by endothelial cells (ECs). In this study, it was discovered that proliferating ECs can upregulate total CaM expression and enhance Ca2+-bound CaM levels in VSMCs co-cultured with ECs. This endothelium-dependent upregulation of CaM in VSMCs is mediated by vascular endothelial growth factor receptors. These findings reveal a new role of ECs in regulating vascular functions through upregulating CaM and its interactions in VSMCs.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Plant Sciences
Xiyang Yang, Xingbing Li, Minghao Luo, Yongzheng Guo, Chang Li, Dingyi Lv, Zhe Cheng, Longxiang Huang, Fei-Fei Shang, Bi Huang, Jian Shen, Suxin Luo, Jianghong Yan
Summary: Tubeimoside I (TBM) promotes angiogenesis through the activation of the eNOS-VEGF signaling pathway, making it a potential novel therapeutic agent for ischemic diseases.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chih-Hsien Wu, Yi-Lin Chiu, Chung-Yueh Hsieh, Guo-Shiang Tsung, Lian-Shan Wu, Cheng-Chung Cheng, Tsung-Neng Tsai
Summary: Cilostazol exerts antithrombotic and vasculoprotective effects in endothelial cells by activating KLF2 and SIRT1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Sofie De Moudt, Jhana O. Hendrickx, Guido R. Y. De Meyer, Wim Martinet, Paul Fransen
Summary: eNOS knockout mice exhibit a slower progression of arterial disease compared to C57Bl/6 control mice, with significant regulation of aortic biomechanical parameters by cyclic stretch amplitudes.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Tao Yan, Shun Guo, Tian Zhang, Zhimin Zhang, An Liu, Song Zhang, Yuan Xu, Yuhong Qi, Weihe Zhao, Qinhui Wang, Lei Shi, Linna Liu
Summary: The study evaluated the protective effects of LIG on radiation enteritis (RE), showing that LIG significantly improved body weights, stool morphologies, intestinal villi lengths, perfusion, and vasodilatory functions in IR rats. LIG also improved Gch1/BH4/eNOS pathway-related proteins and regulatory molecules, indicating it as a preferred compound for the prevention and treatment of RE by improving intestinal ischemia. This study provides new research ideas for the development of new drugs for RE.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Wenhua Lei, Junli Li, Changming Li, Li Chen, Fangyang Huang, Dan Xiao, Jialiang Zhang, Jiahao Zhao, Guoyong Li, Tianyi Qu, Hao Zhou, Yanbiao Liao, Mao Chen
Summary: The study demonstrated that MARCH5 acts as a protective factor in myocardial infarction-related endothelial cell injury and hypoxia-induced endothelial cell injury, potentially through the Akt/eNOS signaling pathway.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Kyung Ha Ku, Michelle K. Dubinsky, Aravin N. Sukumar, Noeline Subramaniam, Manon Y. M. Feasson, Ranju Nair, Eileen Tran, Brent M. Steer, Britta J. Knight, Philip A. Marsden
Summary: The study reveals that the SSRE and KLF elements in the eNOS promoter play critical roles in endothelial cells, providing new mechanistic insights into the transcription of the eNOS gene under chronic shear stress in vivo.
Article
Pharmacology & Pharmacy
Anees Ahmed Syed, Mohammed Shafiq, Mohammad Irshad Reza, Pragya Bharati, Athar Husain, Pragati Singh, Kashif Hanif, Jiaur R. Gayen
Summary: The chronic treatment of Cissus quadrangularis extract improved endothelium-dependent relaxation in spontaneously hypertensive rats by activating eNOS and inhibiting ROS production and inflammation, leading to antihypertensive effects.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2022)
Article
Pharmacology & Pharmacy
Xiaowan Han, Tong Li, Tieshan Wang, Baofu Wang, Yang Li, Lei Wang, Ziwen Lu, Aiming Wu, Lisong Liu, Guozhong Pan, Mingjing Zhao
Summary: This study evaluated the correlation between different microparticle phenotypes and plaque burden, and investigated the role of microparticles in atherosclerosis. The results showed that CD11a(+) LMP and CD11a(+)/CD45(+) LMP may be potential biomarkers for predicting carotid atherosclerosis.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Food Science & Technology
Joseph Festa, Mariasole Da Boit, Aamir Hussain, Harprit Singh
Summary: Cardiovascular disease is the leading cause of global death, with endothelial dysfunction being a predictor for most CVD. Polyphenols from sources like wine and grape extracts can help prevent endothelial dysfunction, while anthocyanin flavonoids found in berries also improve vascular function.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Sarawoot Bunbupha, Patoomporn Prasarttong, Anuson Poasakate, Putcharawipa Maneesai, Poungrat Pakdeechote
Summary: Imperatorin shows therapeutic effects on metabolic and vascular alterations induced by a high-fat/high-fructose diet in rats, potentially through the restoration of adiponectin receptor 1 and eNOS expression and suppression of p47phox expression.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Peripheral Vascular Disease
Chee Lee Wee, Siti Safiah Mokhtar, Kirnpal Kaur Banga Singh, Aida Hanum Ghulam Rasool
Summary: Vitamin D deficiency impairs microvascular vasodilation and reduces antioxidant activity, possibly due to eNOS downregulation. However, calcitriol supplementation does not ameliorate these effects and may even enhance endothelium-dependent contraction, potentially due to hypercalcemia.
MICROVASCULAR RESEARCH
(2021)
Article
Geriatrics & Gerontology
Lulu An, Yi Shen, Michael Chopp, Alex Zacharek, Poornima Venkat, Zhili Chen, Wei Li, Yu Qian, Julie Landschoot-Ward, Jieli Chen
Summary: The study found that eNOS plays an important role in regulating brain damage and cognitive function, and deficiency in eNOS can exacerbate brain damage and cognitive deficits in VaD mouse model.
Article
Biochemistry & Molecular Biology
Richard T. Premont, James D. Reynolds, Rongli Zhang, Jonathan S. Stamler
Summary: Red blood cell-mediated vasodilation is crucial for oxygen delivery, facilitated by the conversion of heme-bound nitric oxide into vasodilator S-nitrosothiol. This process is regulated by a strictly conserved Cys residue in hemoglobin. The model of a three-gas respiratory cycle (O-2/NO/CO2) is supported by the coupling of hemoglobin allostery to NO conversion and SNO release.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Editorial Material
Biochemistry & Molecular Biology
Richard T. Premont
Article
Radiology, Nuclear Medicine & Medical Imaging
Alexandra Badea, Robert Schmalzigaug, Woojoo Kim, Pamela Bonner, Umer Ahmed, G. Allan Johnson, Gary Cofer, Mark Foster, Robert J. Anderson, Cristian Badea, Richard T. Premont
Summary: GIT1-KO mice exhibit neuroimaging phenotypes such as microcephaly and altered cortical layering, with a decrease in neuron density in cortical layer V. High field MRM revealed reduced white matter volume, particularly in the anterior commissure, in GIT1-KO mice. The basal ganglia appeared enlarged in GIT1-KO mice, suggesting a vulnerability to addiction.
MAGNETIC RESONANCE IMAGING
(2021)
Review
Endocrinology & Metabolism
Hua-Lin Zhou, Richard T. Premont, Jonathan S. Stamler
Summary: This review discusses the crucial role of S-nitrosylation in the regulation of insulin signaling throughout the insulin life cycle, impacting both metabolic health and disease. The interplay between insulin signaling and protein S-nitrosylation is highlighted, with aberrant S-nitrosylation contributing to metabolic disorders such as type 2 diabetes mellitus. Understanding the impact of S-nitrosylation on cellular metabolism could provide valuable insights in the field of metabolic signaling and insulin-related disorders.
NATURE REVIEWS ENDOCRINOLOGY
(2022)
Article
Medicine, Research & Experimental
Rongli Zhang, Alfred Hausladen, Zhaoxia Qian, Xudong Liao, Richard T. Premont, Jonathan S. Stamler
Summary: Hemoglobin in red blood cells may play a major role in signaling hypoxia and regulating vascular tone. Mutant mice with reduced SNO-hemoglobin showed impaired hypoxic vasodilation and developed pulmonary hypertension, indicating the importance of red blood cell SNO in the integrated response to hypoxia.
Review
Biochemistry & Molecular Biology
Richard T. Premont, David J. Singel, Jonathan S. Stamler
Summary: This chapter reviews the crucial role of hemoglobin in carrying and releasing SNO in the autoregulation of blood flow, and its physiological and pathological implications for oxygen delivery. Additionally, therapeutic opportunities to optimize oxygen delivery at the level of tissue blood flow are highlighted.
MOLECULAR ASPECTS OF MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Daniel M. Fass, Michael C. Lewis, Rushdy Ahmad, Matthew J. Szucs, Qiangge Zhang, Morgan Fleishman, Dongqing Wang, Myung Jong Kim, Jonathan Biag, Steven A. Carr, Edward M. Scolnick, Richard T. Premont, Stephen J. Haggarty
Summary: This study aims to elucidate the molecular and cellular basis of cognitive deficits in schizophrenia. The research found that genetic variations in a set of genes involved in regulating neuroplasticity may contribute to cognitive deficits and exert their effects by perturbing the networks of these genes.
MOLECULAR PSYCHIATRY
(2022)
Article
Biochemistry & Molecular Biology
Fabio Fonseca, Thomas M. Raffay, Kunhong Xiao, Precious J. McLaughlin, Zhaoxia Qian, Zachary W. Grimmett, Naoko Adachi, Benlian Wang, Alfred Hausladen, Brian A. Cobb, Rongli Zhang, Douglas T. Hess, Benjamin Gaston, Nevin A. Lambert, James D. Reynolds, Richard T. Premont, Jonathan S. Stamler
Summary: S-Nitrosylation is a central mechanism in β(2)-adrenergic receptor signaling, essential for both classic desensitization by PKA and NO-based signaling that mediates bronchorelaxation. Mutant β(2)AR refractory to S-nitrosylation amplifies NO-based signaling, leading to resistance to bronchoconstriction and asthma development.
Article
Cell Biology
Colin T. Stomberski, Nicholas M. Venetos, Hua-Lin Zhou, Zhaoxia Qian, Bryce R. Collison, Seth J. Field, Richard T. Premont, Jonathan S. Stamier
Summary: Accumulating evidence indicates that protein S-nitrosylation is regulated by enzymes, and specificity in S-nitrosylation is derived from dedicated S-nitrosylases and denitrosylases. In this study, mice deficient in the protein denitrosylase SCoR2 showed reduced serum cholesterol due to decreased secretion of the cholesterol-regulating protein PCSK9. The researchers found that SCoR2 controls an S-nitrosylation cascade involving SAR1 and SURF4, which act as S-nitrosylases, to inhibit PCSK9 secretion. These findings suggest a potential treatment paradigm for cardiovascular disease.
Article
Urology & Nephrology
Julian Gerhards, Lars D. Maerz, Edda S. F. Matthees, Cornelia Donow, Barbara Moepps, Richard T. Premont, Martin D. Burkhalter, Carsten Hoffmann, Melanie Philipp
Summary: This study identifies GRK4 as a novel regulator of cilia and kidney development, independent of its kinase function. The GRK4 variants believed to act as hyperactive kinases are dysfunctional for normal ciliogenesis, suggesting a receptor-independent mechanism.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Divya Seth, Colin T. Stomberski, Precious J. McLaughlin, Richard T. Premont, Kathleen Lundberg, Jonathan S. Stamler
Summary: The aim of this study was to investigate the interactions between nitric oxide synthase (NOS) isoforms and proteins, as well as the mechanisms by which they mediate S-nitrosylation. The results showed that each NOS isoform interacts with and S-nitrosylates many proteins, but a large proportion of S-nitrosylated proteins are not associated with NOS. Additionally, the interactomes and S-nitrosylomes of individual NOS isoforms are largely unique, indicating specific roles for each isoform in S-nitrosylation.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Chemistry, Medicinal
Hua-Lin Zhou, Alfred Hausladen, Puneet Anand, Malligarjunan Rajavel, Colin T. Stomberski, Rongli Zhang, Richard T. Premont, William J. Greenlee, Focco van den Akker, Jonathan S. Stamler
Summary: Acute kidney injury (AKI) is a serious condition with high morbidity and mortality, and there are currently no drugs available for treatment. However, the deletion of S-nitrosocoenzyme A reductase 2 (SCoR2; AKR1A1) has been found to protect mice against AKI through metabolic reprogramming, suggesting that SCoR2 could be a potential drug target.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Puneet Seth, Richard T. Premont, Jonathan S. Stamler
Summary: Post-translational modification by S-nitrosylation plays a key role in regulating cellular functions and impacting proteins across phylogeny. The protocol described focuses on isolating S-nitrosylated proteins from C. elegans, with potential for adaptation to mammalian tissues. Protecting SNO proteins from degradation remains a major challenge in this protocol.
Letter
Multidisciplinary Sciences
Songling Liu, Louis M. Luttrell, Richard T. Premont, Don C. Rockey
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Gastroenterology & Hepatology
Kuo Du, Satish K. Chitneni, Ayako Suzuki, Ying Wang, Ricardo Henao, Jeongeun Hyun, Richard T. Premont, Susanna Naggie, Cynthia A. Moylan, Mustafa R. Bashir, Manal F. Abdelmalek, Anna Mae Diehl
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2020)