Article
Microbiology
Deborah A. Ribardo, Jeremiah J. Johnson, David R. Hendrixson
Summary: The study reveals how the flagellar motor of Campylobacter jejuni modulates swimming velocity in different viscosity environments. VidA and VidB are identified as the primary determinants, with VidA being required for swimming in low-viscosity environments and VidB acting as a brake or clutch to slow swimming velocity in low-viscosity environments.
Article
Microbiology
Xiaofei Li, Qinwen Chai, Lina Zheng, Pingyu Huang, Ozan Gundogdu, Xinan Jiao, Yuanyue Tang, Jinlin Huang
Summary: This study demonstrated the critical role of the B and N domains of FlhF protein in flagellar assembly in Campylobacter jejuni. Point mutations in these domains caused abnormal flagella, disrupted GTPase activity and polar targeting of FlhF, and significantly affected flagella gene expression and transcription.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Claudia A. Cox, Marek Bogacz, Faiha M. El Abbar, Darren D. Browning, Brian Y. Hsueh, Chris M. Waters, Vincent T. Lee, Stuart A. Thompson
Summary: Campylobacter jejuni is a leading cause of bacterial gastroenteritis and is associated with various extragastrointestinal conditions. The study found that CbrR is a negative regulator that controls motility and the expression of FlaA, a critical virulence factor in C. jejuni pathogenesis.
Article
Multidisciplinary Sciences
Xiaofang Wu, Lei Ji, Yuehua Shen, Liping Chen, Deshun Xu, Fenfen Dong
Summary: Thirteen pathogenic Campylobacter strains were isolated from diarrhea cases and raw poultry meat products in Huzhou, including 11 Campylobacter jejuni and 2 Campylobacter coli strains. These strains contain multiple virulence-related genes and show high resistance to nalidixic acid, ciprofloxacin, and tetracycline. Genomic sequencing revealed the presence of tet(O) and blaOXA resistance genes in all C. jejuni strains and multiple drug resistance genes in 2 C. coli strains.
Article
Multidisciplinary Sciences
Anand B. Karki, Kaylee Ballard, Claudia Harper, Robert J. Sheaff, Mohamed K. Fakhr
Summary: This study found that the presence of Staphylococcus aureus can enhance the survival of Campylobacter in adverse conditions such as low temperature and aerobic conditions, especially during biofilm formation. However, the results were inconclusive when extracts from cell-free media of S. aureus were used.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Shuxin Zhang, Jiahua Shi, Xuan Li, Lachlan Coin, Jake W. O'Brien, Muttucumaru Sivakumar, Faisal Hai, Guangming Jiang
Summary: This study developed a triplex qPCR assay for the detection and quantification of Campylobacter species in wastewater samples, specifically targeting C. jejuni and C. coli. The assay demonstrated high sensitivity and reliability, and can be used for long-term monitoring of Campylobacter prevalence in communities and surrounding environments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Medelin Ocejo, Beatriz Oporto, Jose Luis Lavin, Ana Hurtado
Summary: A long-term surveillance study on dairy cattle farms revealed that C. jejuni was more prevalent than C. coli, and resistance rates were higher in calves. Some genotypes persisted throughout the study while others were sporadically detected. Acquisition of extracellular genes and intracellular mutational events were identified as the processes that led to the emergence and spread of resistant genotypes. Oxford Nanopore Technologies sequencing helped decipher the complex molecular epidemiology of resistant Campylobacter dissemination.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Nikola Ondrikova, Helen E. Clough, Amy Douglas, Miren Iturriza-Gomara, Lesley Larkin, Roberto Vivancos, John P. Harris, Nigel A. Cunliffe
Summary: The COVID-19 pandemic had a more adverse impact on laboratory reporting of norovirus compared to Campylobacter in England. This may be explained by stronger behavioral interventions affecting norovirus transmission and a greater reduction in norovirus testing capacity. The study highlights the differential impact a pandemic can have on surveillance of gastrointestinal infectious diseases.
Article
Microbiology
Anand B. Karki, Bhuwan Khatri, Mohamed K. Fakhr
Summary: RNA-seq was used to analyze the differentially expressed genes (DEGs) in C. coli HC2-48 and C. jejuni OD2-67 transcriptomes under different temperature conditions. In C. coli HC2-48, genes involved in flagellar assembly were significantly impacted by low temperatures (4°C), while genes related to the ribosome and ribonucleoprotein complex were modulated in C. jejuni OD2-67 at 4°C. Although more DEGs were observed in the chicken juice medium compared to Mueller-Hinton broth, there were no common genes expressed at all incubation times, indicating that the food matrix environment is not the sole determinant of differential expression in Campylobacter spp. at low temperatures.
Article
Veterinary Sciences
Debora Brito Goulart, Qijing Zhang, Orhan Sahin
Summary: Human enterocolitis is commonly caused by the Gram-negative bacterium Campylobacter jejuni. The emergence of fluoroquinolone-resistant (FQ-R) Campylobacter has increased in poultry and cattle, which are important reservoirs of the bacteria. In this study, it was found that FQ-R Campylobacter strains from cattle had slightly higher fitness advantage over susceptible strains, but the emergence of FQ-R mutants was primarily influenced by bacterial cell density and antibiotic concentration. These findings provide explanations for the prevalence of FQ-R Campylobacter in cattle production and the limited development of FQ-R Campylobacter in response to treatment in cattle intestines.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Leon Kemper, Andreas Hensel
Summary: Campylobacter jejuni, causing severe enteritis, has unique features and adapts effectively to environmental conditions. The review summarizes the strategies of C. jejuni for host-pathogen interaction, providing insights for future drug development.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Pengxiang Li, Yifang Cui, Fangfang Guo, Jiahui Guo, Xiaoya Cao, Jun Lin, Baoan Ding, Fuzhou Xu
Summary: This study aimed to characterize the chicken host defense peptides (HDPs) responses to Campylobacter jejuni (C. jejuni) infection. In vitro experiments showed that C. jejuni infection suppressed the expression of certain HDPs but increased the expression of others. In vivo experiments in chickens demonstrated that C. jejuni infection initially up-regulated the expression of HDPs, but this expression decreased over time. The findings suggest that C. jejuni evades the chicken immune system by suppressing the expression of chicken HDPs.
VETERINARY MICROBIOLOGY
(2023)
Review
Microbiology
Walid Ghazi Al Hakeem, Shahna Fathima, Revathi Shanmugasundaram, Ramesh K. Selvaraj
Summary: C. jejuni is the leading cause of human foodborne illness associated with poultry, beef, and pork consumption. Horizontal transmission from the environment is considered to be the primary source of C. jejuni in commercial poultry farms. C. jejuni expresses virulence factors regulated by a two-component system that mediates its ability to survive in the host.
Article
Microbiology
Andrea Laconi, Ilenia Drigo, Nicola Palmieri, Lisa Carraro, Elena Tonon, Raffaela Franch, Luca Bano, Alessandra Piccirillo
Summary: The study revealed that Campylobacter isolates from chicken livers possessed a higher number of virulence-associated genes, which might contribute to the development of pathological lesions in these isolates within the host.
VETERINARY MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Shuxin Zhang, Jiahua Shi, Elipsha Sharma, Xuan Li, Shuhong Gao, Xu Zhou, Jake O'Brien, Lachlan Coin, Yanchen Liu, Muttucumaru Sivakumar, Faisal Hai, Guangming Jiang
Summary: This study investigated the recovery of Campylobacter spp. from wastewater and their decay under different simulated sewer reactor conditions. The results showed that the recovery of Campylobacter spp. varied with their concentrations in wastewater and the detection limit of quantification methods. The concentration reduction of Campylobacter spp. in sewers followed a two-phase reduction model, with the faster reduction in the first phase mainly due to partitioning onto sewer biofilms. The total decay of Campylobacter spp. varied in different types of sewer reactors.
Article
Biochemistry & Molecular Biology
Reuben Ha, Emilisa Frirdich, David Sychantha, Jacob Biboy, Michael E. Taveirne, Jeremiah G. Johnson, Victor J. DiRita, Waldemar Vollmer, Anthony J. Clarke, Erin C. Gaynor
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Microbiology
Jeremiah G. Johnson, Jennifer A. Gaddy, Victor J. Dirita
Review
Microbiology
Tylor J. Johnson, Janette M. Shank, Jeremiah G. Johnson
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Immunology
Janette M. Shank, Brittni R. Kelley, Joseph W. Jackson, Jessica L. Tweedie, Dana Franklin, Steven M. Damo, Jennifer A. Gaddy, Caitlin N. Murphy, Jeremiah G. Johnson
INFECTION AND IMMUNITY
(2018)
Article
Microbiology
Jeremiah G. Johnson, Jonathan Livny, Victor J. DiRita
JOURNAL OF BACTERIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Emilisa Frirdich, Jenny Vermeulen, Jacob Biboy, Fraser Soares, Michael E. Taveirne, Jeremiah G. Johnson, Victor J. DiRita, Stephen E. Girardin, Waldemar Vollmer, Erin C. Gaynor
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Review
Immunology
Kaylie L. Richard, Brittni R. Kelley, Jeremiah G. Johnson
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2019)
Article
Microbiology
Deborah A. Ribardo, Brittni R. Kelley, Jeremiah G. Johnson, David R. Hendrixson
Article
Cell Biology
Sean Callahan, Ryan S. Doster, Joseph W. Jackson, Brittni R. Kelley, Jennifer A. Gaddy, Jeremiah G. Johnson
CELLULAR MICROBIOLOGY
(2020)
Article
Microbiology
Brittni R. Kelley, Sean M. Callahan, Jeremiah G. Johnson
Summary: The transcriptional regulator HeuR in Campylobacter jejuni plays a role in positively regulating heme utilization and negatively regulating methionine biosynthesis. Mutations in HeuR result in overexpression of metC, impacting bacterial growth and invasion ability under nutrient-replete conditions and iron limitation. This study highlights the importance of regulating methionine biosynthesis for adherence and invasion in the gastrointestinal tract.
JOURNAL OF BACTERIOLOGY
(2021)
Review
Immunology
Sean M. Callahan, Carolina G. Dolislager, Jeremiah G. Johnson
Summary: Campylobacter spp. are the leading cause of bacterium-derived gastroenteritis worldwide, impacting 96 million individuals annually. The resulting gastrointestinal disease and postinfectious disorders are believed to be primarily due to the host immune response toward the bacterium, which may lead to dysregulation or misdirection of the inflammatory response. Understanding the cellular immune responses toward Campylobacter is increasingly important for human health, including critical immunological events for disease development and postinfectious disorders.
INFECTION AND IMMUNITY
(2021)
Review
Biochemistry & Molecular Biology
Brittni R. Kelley, Jacky Lu, Kathryn P. Haley, Jennifer A. Gaddy, Jeremiah G. Johnson
Summary: Epsilonproteobacteria is a diverse class of eubacteria within the Proteobacteria phylum, including environmental sulfur-reducing bacteria and human pathogens that infect multiple animal hosts, causing various disease outcomes. Hosts have evolved strategies to sequester metals from pathogens or exploit metal toxicity for antimicrobial purposes, while the pathogens have developed mechanisms to sense metal availability changes and regulate their physiology in response.
Article
Microbiology
Jeremiah G. Johnson, Rachel R. Spurbeck, Sukhinder K. Sandhu, Jyl S. Matson
GENOME ANNOUNCEMENTS
(2014)
Article
Jeremiah G. Johnson, Samuel Carpentier, Rachel R. Spurbeck, Sukhinder K. Sandhu, Victor J. DiRita
GENOME ANNOUNCEMENTS
(2014)