Article
Multidisciplinary Sciences
Takenobu Katagiri, Sho Tsukamoto, Mai Kuratani, Shinnosuke Tsuji, Kensuke Nakamura, Satoshi Ohte, Yoshiro Kawaguchi, Kiyosumi Takaishi
Summary: Mutations in the transmembrane receptor activin receptor-like kinase 2 (ALK2) cause abnormal signaling in various genetic diseases. The authors found that disease-associated ALK2 forms can dimerize intracellularly upon ligand binding. They developed a blocking antibody that inhibits ALK2 signaling and demonstrated its potential in suppressing pathological bone formation in a mouse model of fibrodysplasia ossificans progressiva.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Melissa Girard, Steve Dagenais Bellefeuille, Emilie Eiselt, Guillaume Arguin, Jean-Michel Longpre, Philippe Sarret, Fernand-Pierre Gendron
Summary: This study found that endosomal trafficking plays an important role in G protein-coupled receptor (GPCR) signaling. UDP selectively activates GPCR P2Y6 as a signaling molecule. The study found that MRS2693 and UDP have different effects on the internalization of the P2Y6 receptor, with MRS2693 inducing P2Y6 internalization through an independent caveolin-dependent mechanism. This study provides insights for the development of bias ligands that can influence P2Y6 signaling.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Physics, Fluids & Plasmas
Paul C. Bressloff
Summary: In this paper, the relaxation to steady state of intracellular diffusion in a pair of cells with gap junction coupling is analyzed. The local accumulation time is calculated and it is found to be a monotonically increasing function of spatial position. Both static and dynamic gap junction models are considered.
Article
Multidisciplinary Sciences
Sumin Lu, Wonjo Jang, Asuka Inoue, Nevin A. Lambert
Summary: A study aimed to determine the G protein coupling profiles of orphan GPCRs using constitutive receptor activity. Out of 48 understudied orphan GPCRs, 22 were found to have sufficient GDP-sensitive bioluminescence resonance energy transfer (BRET) to generate G protein coupling profiles. A total of 48 coupled receptor-G protein pairs were identified, many of which were previously undescribed. This approach may be useful for studying G protein coupling of other GPCRs lacking activating ligands.
Review
Microbiology
Tran X. N. Huy, Trang T. Nguyen, Heejin Kim, Alisha W. B. Reyes, Suk Kim
Summary: Brucella species is the causative agent of brucellosis, causing significant economic losses and public health threats. Brucella invades host cells and survives through pathogen-host interactions, utilizing various mechanisms to evade immune responses.
Article
Cell Biology
Weixiang Bian, Hua Jiang, Luxia Yao, Wanyu Hao, Lianfeng Wu, Xu Li
Summary: The Notch signaling pathway controls cell growth, differentiation, and fate decisions, but dysregulation of this pathway is linked to various human diseases. By using TurboID-based proximity labeling, the researchers establish a spatially defined human Notch receptor interaction network, revealing how Notch receptors interact with different proteins in distinct cellular compartments. This study provides insights into the spatial regulation of Notch receptors and highlights potential therapeutic targets for Notch-related diseases.
Article
Pharmacology & Pharmacy
Nicole Urban, Marion Leonhardt, Michael Schaefer
Summary: This study identified and characterized novel agonists of GPER1/GPR30 receptor, showing that GPR30 couples to the canonical Gq-phospholipase C pathway and undergoes rapid internalization upon continuous exposure to the agonists. These findings provide insights into the physiological responses controlled by GPR30.
MOLECULAR PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qiufeng Liu, Dehua Yang, Youwen Zhuang, Tristan I. Croll, Xiaoqing Cai, Antao Dai, Xinheng He, Jia Duan, Wanchao Yin, Chenyu Ye, Fulai Zhou, Beili Wu, Qiang Zhao, H. Eric Xu, Ming-Wei Wang, Yi Jiang
Summary: CCKAR is a G-protein-coupled receptor that regulates nutrient homeostasis by interacting with a sulfated ligand and coupling with different G-protein subtypes. The selectivity of G-protein coupling is determined by conformational differences in the G alpha subunits' 'wavy hook' and the ICL3 of the receptor.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Bernhard C. Lechtenberg, Marina P. Gehring, Taylor P. Light, Christopher R. Horne, Mike W. Matsumoto, Kalina Hristova, Elena B. Pasquale
Summary: The study reveals that accumulation of multiple negative charges mimicking phosphorylation induces cooperative changes in the EphA2 intracellular region, promoting oligomerization and perturbing the juxtamembrane segment and kinase domain. Multiple kinases are identified as catalyzing linker phosphorylation, suggesting complex effects and the necessity for coordination of different kinases in promoting EphA2 non-canonical signaling.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Alexander N. Kapustin, Paul Davey, David Longmire, Carl Matthews, Emily Linnane, Nitin Rustogi, Maria Stavrou, Paul W. A. Devine, Nicholas J. Bond, Lyndsey Hanson, Silvia Sonzini, Alexey Revenko, A. Robert MacLeod, Sarah Ross, Elisabetta Chiarparin, Sanyogitta Puri
Summary: Researchers investigated the intracellular trafficking of anti-KRAS antisense oligonucleotides, showing that oligonucleotide AZD4785 is recycled via late endosomes in extracellular vesicles in both cells with poor and good oligo productive uptake. Inducing lysobisphosphatidic acid in late endosomes or blocking EV recycling can enhance AZD4785 activity in cells with poor productive uptake, potentially offering improved treatment strategies.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kelly J. Culhane, Tejas M. Gupte, Indrani Madhugiri, Chetan J. Gadgil, Sivaraj Sivaramakrishnan
Summary: This study proposes a kinetic model to simulate the interactions between hormone, G protein coupled receptor (GPCR), and G protein. Experimental validation reveals the phenomenon of GPCR priming and the temporary enhancement of GPCR ternary complex formation by allokairic effectors. This study uncovers the kinetic mechanisms of ternary complex formation and downstream signaling.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Maxemiliano V. Vargas, Lee E. Dunlap, Chunyang Dong, Samuel J. Carter, Robert J. Tombari, Shekib A. Jami, Lindsay P. Cameron, Seona D. Patel, Joseph J. Hennessey, Hannah N. Saeger, John D. McCorvy, John A. Gray, Lin Tian, David E. Olson
Summary: Decreased dendritic spine density in the cortex is a common feature of neuropsychiatric diseases, and psychedelics have been found to promote cortical neuron growth. This study shows that intracellular 5-HT2A receptors mediate the plasticity-promoting effects of psychedelics, providing insight into the mechanisms behind their therapeutic effects. The findings suggest that targeting intracellular 5-HT2A receptors could be a potential therapeutic approach.
Article
Biochemistry & Molecular Biology
Yulin Tian, Ming Fang, Qing Lin
Summary: The intracellular fluorescent labeling of GCGR ICL3 using a bioorthogonal chemistry strategy was achieved successfully, but faced challenges in live-cell imaging studies due to the interference of specific and non-specific signals. Further research is needed to overcome these challenges and improve the specificity of the labeling reactions.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Review
Neurosciences
Kelly G. Sullivan, Greg J. Bashaw
Summary: Friedrich Bonhoeffer made significant contributions to the study of axon guidance in the developing nervous system by uncovering key cellular and molecular mechanisms and cloning important repulsive axon guidance cues. This review highlights his major contributions and ongoing investigations into the regulation of axon repulsion. The post-translational regulation of ephrin and slit ligands and their respective receptors is discussed, with a focus on endocytic trafficking events and receptor signaling. The article aims to identify outstanding questions for future research. Published by Elsevier Ltd. as part of a Special Issue entitled "Prof. Friedrich Bonhoeffer". All rights reserved.
Article
Multidisciplinary Sciences
Xin Xu, Nevin A. Lambert, Guangyu Wu
Summary: This study reveals that GPCRs can be actively captured by COPII via specific motifs and direct interaction with COPII components, which in turn affects their export dynamics. This provides important insights into COPII targeting and forward trafficking of nascent GPCRs.
Article
Biochemistry & Molecular Biology
Yaguang Zhao, Jiayu Wu, Hong Jia, Xinying Wang, Ruizhi Zheng, Fang Jiang, Dan-Na Chen, Zhiheng Chen, Jia-Da Li
Article
Medicine, Research & Experimental
Meichao Men, Wu Li, Hongsheng Chen, Jiayu Wu, Yong Feng, Hui Guo, Jia-Da Li
Article
Endocrinology & Metabolism
Fangfang Li, Dengfeng Li, Huadie Liu, Bei-Bei Cao, Fang Jiang, Dan-Na Chen, Jia-Da Li
FRONTIERS IN ENDOCRINOLOGY
(2019)
Article
Obstetrics & Gynecology
Meichao Men, Jiayu Wu, Yaguang Zhao, Xiaoliang Xing, Fang Jiang, Ruizhi Zheng, Jia-Da Li
FERTILITY AND STERILITY
(2020)
Article
Endocrinology & Metabolism
Jia-Da Li, Jiayu Wu, Yaguang Zhao, Xinying Wang, Fang Jiang, Qiao Hou, Dan-Na Chen, Ruizhi Zheng, Renhe Yu, Wei Zhou, Meichao Men
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2020)
Article
Genetics & Heredity
Wenting Dai, Jia-Da Li, Yaguang Zhao, Jiayu Wu, Fang Jiang, Dan-Na Chen, Ruizhi Zheng, Meichao Men
Article
Neurosciences
Xiaoliang Xing, Kunyang Wu, Yufan Dong, Yimei Zhou, Jing Zhang, Fang Jiang, Wang-Ping Hu, Jia-Da Li
Article
Multidisciplinary Sciences
Ye Liang, ShanShan Yuan, JingJing Huan, HuiXin Wang, YiYi Zhang, ChangYun Fang, Jia-Da Li
Article
Cell Biology
Zongyang Lu, Zhen Liu, Wei Mao, Xinying Wang, Xiaoguo Zheng, Shanshan Chen, Beibei Cao, Shisheng Huang, Xuliang Zhang, Tao Zhou, Yu Zhang, Xingxu Huang, Qiang Sun, Jia-Da Li
CELL DEATH & DISEASE
(2020)
Article
Genetics & Heredity
Meichao Men, Xinying Wang, Jiayu Wu, Wang Zeng, Fang Jiang, Ruizhi Zheng, Jia-Da Li
Summary: The study investigated genotypic and phenotypic spectra of DUSP6, IL17RD, and SPRY4 in a large cohort of Chinese patients with IHH. Mutations in DUSP6 alone could cause IHH in an autosomal dominant manner, while IL17RD or SPRY4 mutations may synergistically lead to IHH phenotypes with variants in other IHH-associated genes.
JOURNAL OF MEDICAL GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Renbin Lu, Yufan Dong, Jia-Da Li
NUCLEIC ACIDS RESEARCH
(2020)
Article
Endocrinology & Metabolism
Qiao Hou, Jiayu Wu, Yaguang Zhao, Xinying Wang, Fang Jiang, Dan-Na Chen, Ruizhi Zheng, Meichao Men, Jia-Da Li
EUROPEAN JOURNAL OF ENDOCRINOLOGY
(2020)
Article
Multidisciplinary Sciences
Ye Liang, JingJing Huan, Jia-Da Li, CanHua Jiang, ChangYun Fang, YongGang Liu
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Dengfeng Li, Fangfang Li, Lanlan Meng, Huafang Wei, Qianjun Zhang, Fang Jiang, Dan-Na Chen, Wei Li, Yue-Qiu Tan, Jia-Da Li
Summary: The study demonstrated that RNF216 plays a crucial role in regulation of meiosis during spermatogenesis in mice, with its deficiency leading to incomplete arrest of zygotene spermatocytes and apoptosis at the pachytene stage. Additionally, RNF216 was shown to interact with PRKACB and promote its degradation, resulting in elevated PKA activity in the testes.
Article
Multidisciplinary Sciences
Xinyu Li, Yufeng Cai, Yinghe Ding, Jia-Da Li, Guoqing Huang, Ye Liang, Linyong Xu
Summary: This study aimed to estimate the demand for isolation beds during the COVID-19 pandemic. A discrete simulation model was established to analyze the impact of various factors such as incubation period, hospital response speed, disease healing time, and population mobility on the epidemic and medical resource occupation. Multiple linear regression was used to predict the number of isolation beds, and the results showed that these factors significantly influenced the demand and number of beds.