Article
Microbiology
Mengqing Zhou, Yuping Huang, Yuanxing Zhang, Qiyao Wang, Yue Ma, Shuai Shao
Summary: This study found that under alkaline pH nutrient limitation, a subpopulation of Vibrio alginolyticus cells enters the VBNC state with pivotal virulence regulator ToxR playing a key role. ToxR directly controls the expression of multiple genes, including those involved in ROS resistance, to regulate the formation of the VBNC state, leading to decreased virulence and activity when ToxR is impaired.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Wenjie Zhou, Yingying Li, Zhuobo Li, Bo Ma, Xiao Jiang, Chaoqun Hu, Yongxing Ai, Peng Luo
Summary: A study found that under phage infection stress, there were numerous mutations and genetic divergence in the genomes of Vibrio alginolyticus PRM strains and coevolved cell populations.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Huan Liu, Miao Xiao, Jing Zuo, Xiaoxian He, Ping Lu, Yingyu Li, Yanni Zhao, Fei Xia
Summary: This study found that vanillic acid exhibited good antibacterial activity against the fish pathogen Vibrio alginolyticus, causing cell membrane damage and increasing membrane permeability in a dose-/time-dependent manner. Additionally, vanillic acid significantly reduced biofilm formation, mobility, exotoxin production, and downregulated virulence-associated genes in V. alginolyticus. These findings suggest that vanillic acid may be a promising agent for treating infections associated with V. alginolyticus in aquaculture.
JOURNAL OF FISH DISEASES
(2021)
Article
Microbiology
Xiao-Xiao Gong, Yan-Hua Zeng, Hai-Min Chen, Na Zhang, Yue Han, Hao Long, Zhen-Yu Xie
Summary: This study found that c-di-GMP metabolic proteins can regulate different phenotypic outputs in Vibrio alginolyticus, a dominant marine pathogen, and overexpression of certain proteins enhances biofilm matrix production. The high abundance of c-di-GMP metabolic proteins suggests the importance of c-di-GMP signaling in the response and adaptation of V. alginolyticus to the marine environment. This work provides a foundation for further research on the molecular mechanisms of c-di-GMP in biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Michio Homma, Akira Mizuno, Yuxi Hao, Seiji Kojima
Summary: This study reveals that GTPase FlhF and ATPase FlhG are critical factors in regulating flagellum number in Vibrio alginolyticus. The N-terminal region of FlhG controls the flagellum number by adjusting FlhF activity and the amount of FlhG.
JOURNAL OF BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Grigori Y. Rychkov, Zeeshan Shaukat, Chiao Xin Lim, Rashid Hussain, Ben J. Roberts, Claudia M. Bonardi, Guido Rubboli, Brandon F. Meaney, Robyn Whitney, Rikke S. Moller, Michael G. Ricos, Leanne M. Dibbens
Summary: The effects of various KCNT1 mutations on the severity of neurological disorders were investigated, and a positive correlation was found between the severity of the disorder and the KCNT1 channel open probability at resting membrane potential. This suggests that gain of function KCNT1 mutations cause epilepsy by increasing resting potassium conductance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pablo Avalos Prado, Stephanie Hafner, Yannick Comoglio, Brigitte Wdziekonski, Christophe Duranton, Bernard Attali, Jacques Barhanin, Guillaume Sandoz
Summary: The study reveals the role of KCN E1 as a bona fide auxiliary subunit in the TMEM16A chloride channel, changing the ion conduction property of the channel. Genetic mutations in KCN E1 can affect the regulation of TMEM16A, leading to inherited pathologies.
Article
Cell Biology
Rui Tang, Emily G. G. Shuldiner, Marcus Kelly, Christopher W. W. Murray, Jess D. D. Hebert, Laura Andrejka, Min K. K. Tsai, Nicholas W. W. Hughes, Mitchell I. I. Parker, Hongchen Cai, Yao-Cheng Li, Geoffrey M. M. Wahl, Roland L. L. Dunbrack, Peter K. K. Jackson, Dmitri A. A. Petrov, Monte M. M. Winslow
Summary: Using CRISPR/Cas9 technology, Tang et al. show that HRAS and NRAS suppress the growth of KRAS-driven lung cancer by inhibiting KRAS-KRAS interactions and reducing downstream ERK signaling. The study highlights the specific suppressive role of RAS paralogues in KRAS-driven lung cancer, as compared to BRAF-driven lung cancer.
NATURE CELL BIOLOGY
(2023)
Article
Fisheries
Hong-Jin Li, Bin-Tong Yang, Yu-Feng Sun, Tong Zhao, Zhi-Peng Hao, Wei Gu, Meng-Xia Sun, Wei Cong, Yuan-Huan Kang
Summary: Vibrio mimicus is a pathogenic bacterium that causes diseases in humans and aquatic animals. This study focused on the development and evaluation of two recombinant Lactobacillus casei strains as oral vaccines against V. mimicus in Carassius auratus. The results showed that the recombinant L. casei strains could effectively stimulate both humoral and cellular immunity in C. auratus, leading to increased levels of specific immunoglobulin M and enhanced activity of various immune-related enzymes and molecules. Importantly, the vaccinated fish exhibited higher survival rates when challenged with V. mimicus. The study suggests that the Lc-pPG-OmpK-CTB strain is an effective candidate for oral vaccination.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anish Kumar Mondal, Paras Verma, Nayanika Sengupta, Somnath Dutta, Shashi Bhushan Pandit, Kausik Chattopadhyay
Summary: This study demonstrates the crucial role of a key tyrosine residue (Y321) in the hinge region of the pore-forming motif of Vibrio cholerae cytolysin in regulating membrane binding, structural rearrangements, oligomerization, and pore-formation, providing insights into the molecular mechanism of beta-barrel pore-forming toxins.
MOLECULAR MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Haixia Zhang, Alex Hanson, Tobias Scherf de Almeida, Christopher Emfinger, Conor McClenaghan, Theresa Harter, Zihan Yan, Paige E. Cooper, G. Schuyler Brown, Eric C. Arakel, Robert P. Mecham, Atilla Kovacs, Carmen M. Halabi, Blanche Schwappach, Maria S. Remedi, Colin G. Nichols
Summary: Cantu syndrome is caused by gain-of-function mutations in potassium channel subunits. An experiment with CRISPR/Cas9 found that R1154Q mutation led to lower K-ATP current density in cardiomyocytes and vascular smooth muscle. Surprisingly, there were distinct SUR2 transcripts, one encoding full-length protein and one with an in-frame deletion, present in R1154Q tissues.
Review
Biochemistry & Molecular Biology
Alanah Varricchio, Sunita A. Ramesh, Andrea J. Yool
Summary: Glioblastoma multiforme is a leading cause of brain cancer-related deaths worldwide, with cells capable of infiltrating healthy brain tissue rapidly. Targeting cellular motility pathways may help slow tumor dispersal and provide a longer time window for frontline treatments. Expressions of Aquaporins (AQPs) and voltage-gated ion channels in GBM cells could be pharmacological targets to restrain cell migration, potentially enhancing therapeutic effectiveness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
W. Wang, S. Guo, Y. Gao, X. Liang, L. Liu, S. Pan
Summary: The study indicated limitations in using recombinant OmpK for preparing diagnostic antibodies, while the serum against formalin and heat-killed V. parahaemolyticus showed broad reactivity, potentially useful for diagnosis and vaccine development.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Hao Zhang, Duan Ni, Jigang Fan, Minyu Li, Jian Zhang, Chen Hua, Ruth Nussinov, Shaoyong Lu
Summary: This study reveals the transition pathway of the C-terminal hypervariable region (HVR) of K-Ras4B, identifies key conformational substates, and uncovers important interactions between the HVR and the catalytic domain. These findings are essential for a deeper understanding of the HVR transition mechanism and the regulation of K-Ras4B activity.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Materials Science, Multidisciplinary
Soumendra Prasad Rout, Pradipta Dutta, Subir Kumar Maity
Summary: This paper analyzes the logic performance of a hybrid CMOS circuit based on a novel technology and compares it with Si-based CMOS circuit. The proposed structure shows improved circuit performance. Furthermore, the design and performance of universal logic gates are also studied.
ECS JOURNAL OF SOLID STATE SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marina Diskowski, Vedrana Mikusevic, Charlott Stock, Inga Haenel
BIOLOGICAL CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Kristina Halbleib, Kristina Pesek, Roberto Covino, Harald F. Hofbauer, Dorith Wunnicke, Inga Haenelt, Gerhard Hummer, Robert Ernst
Article
Biology
Marina Diskowski, Ahmad Reza Mehdipour, Dorith Wunnicke, Deryck J. Mills, Vedrana Mikusevic, Natalie Baerland, Jan Hoffmann, Nina Morgner, Heinz-Juergen Steinhoff, Gerhard Hummer, Janet Vonck, Inga Haenelt
Review
Crystallography
Dorith Wunnicke, Inga Haenelt
Article
Multidisciplinary Sciences
C. Stock, L. Hielkema, I. Tascon, D. Wunnicke, G. T. Oostergetel, M. Azkargorta, C. Paulino, I. Haenelt
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Jan Gundlach, Larissa Krueger, Christina Herzberg, Asan Turdiev, Anja Poehlein, Igor Tascon, Martin Weiss, Dietrich Hertel, Rolf Daniel, Inga Haenelt, Vincent T. Lee, Joerg Stuelke
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Marina Schrecker, Dorith Wunnicke, Inga Hanelt
BIOLOGICAL CHEMISTRY
(2019)
Article
Physiology
Vedrana Mikusevic, Marina Schrecker, Natalie Kolesova, Miyer Patino-Ruiz, Klaus Fendler, Inga Haenelt
JOURNAL OF GENERAL PHYSIOLOGY
(2019)
Article
Multidisciplinary Sciences
Stephanie Ballweg, Erdinc Sezgin, Milka Doktorova, Roberto Covino, John Reinhard, Dorith Wunnicke, Inga Haenelt, Ilya Levental, Gerhard Hummer, Robert Ernst
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Igor Tascon, Joana S. Sousa, Robin A. Corey, Deryck J. Mills, David Griwatz, Nadine Aumueller, Vedrana Mikusevic, Phillip J. Stansfeld, Janet Vonck, Inga Haenelt
NATURE COMMUNICATIONS
(2020)
Article
Microbiology
Larissa Krueger, Christina Herzberg, Robert Warneke, Anja Poehlein, Janina Stautz, Martin Weiss, Roif Daniel, Inga Haenelt, Jorg Stuelke
JOURNAL OF BACTERIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Janina Stautz, Yvonne Hellmich, Michael F. Fuss, Jakob M. Silberberg, Jason R. Devlin, Randy B. Stockbridge, Inga Haenelt
Summary: Potassium ion homeostasis is vital for bacterial survival, with multiple uptake and release systems allowing bacteria to adapt to various stress situations. Understanding the molecular details of these systems' functions is crucial for microbiologists in studying how they coordinate to maintain potassium balance in bacteria.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jakob M. Silberberg, Robin A. Corey, Lisa Hielkema, Charlott Stock, Phillip J. Stansfeld, Cristina Paulino, Inga Haenelt
Summary: KdpFABC is a high-affinity bacterial K+ pump which combines the ion channel-like KdpA and the P-type ATPase KdpB for ion transport via an intersubunit tunnel. The transport mechanism involves a phenylalanine constriction and charge distribution for ATPase coupling. Elements of this mechanism are conserved among different families of P-type ATPases, suggesting a common ancestor for KdpB.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Jakob M. Silberberg, Charlott Stock, Lisa Hielkema, Robin A. Corey, Jan Rheinberger, Dorith Wunnicke, Victor R. A. Dubach, Phillip J. Stansfeld, Inga Haenelt, Cristina Paulino
Summary: This study explores the structural basis of inhibition by KdpB(S162) phosphorylation in KdpFABC under different conditions. A new inhibited state termed E1P tight is identified, which likely reflects a stalled state adopted by KdpFABC upon KdpB(S162) phosphorylation during high K+ levels. The compact fold of the cytoplasmic domains in the E1P tight state suggests a potential mechanism when the transition to E2P states is unsuccessful.
Article
Chemistry, Multidisciplinary
Nils Hellwig, Oliver Peetz, Zainab Ahdash, Igor Tascon, Paula J. Booth, Vedrana Mikusevic, Marina Diskowski, Argyris Politis, Yvonne Hellmich, Inga Haenelt, Eamonn Reading, Nina Morgner
CHEMICAL COMMUNICATIONS
(2018)