Article
Biochemistry & Molecular Biology
Fenggui Fan, Hao Zhang, Qian Wei, Yahui Wei
Summary: This study reveals the evolutionary conservation of PDI-S between land plants and algal organisms, and elucidates the induction mechanism of AtPDI11 under ER stress and the crucial importance of the D domain for its activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sujun Chen, Guanghui Zhu, Yue Yang, Fubo Wang, Yu-Tian Xiao, Na Zhang, Xiaojie Bian, Yasheng Zhu, Yongwei Yu, Fei Liu, Keqin Dong, Javier Mariscal, Yin Liu, Fraser Soares, Helen Loo Yau, Bo Zhang, Weidong Chen, Chao Wang, Dai Chen, Qinghua Guo, Zhengfang Yi, Mingyao Liu, Michael Fraser, Daniel D. De Carvalho, Paul C. Boutros, Dolores Di Vizio, Zhou Jiang, Theodorus van der Kwast, Alejandro Berlin, Song Wu, Jianhua Wang, Housheng Hansen He, Shancheng Ren
Summary: Chen et al. conducted single-cell analysis to identify transcriptomic remodeling in various cell types that regulate prostate cancer progression. They found that the tumor microenvironment displayed activation of progression-associated transcriptomic programs and identified a subset of endothelial cells that actively communicate with tumor cells, promoting cancer cell invasion. Their study also revealed promiscuous KLK3 expression and the ability of cancer cells to alter T-cell transcriptomes.
NATURE CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yaning Li, Qing Fan, Bing Zhou, Yaping Shen, Yuanyuan Zhang, Lin Cheng, Furong Qi, Shuo Song, Yingying Guo, Renhong Yan, Bin Ju, Zheng Zhang
Summary: This article explores the structure and function of STS165, a broadly inter-Spike bivalent neutralizing antibody (nAb) against SARS-CoV-2 variants and even SARS-CoV, contributing to further understanding of the working mechanism of nAbs.
Article
Cell Biology
Gemma Chiva-Blanch, Alba Vilella-Figuerola, Teresa Padro, Francesc Formiga, Assumpta Ferrer, Lina Badimon
Summary: Successful aging is associated with lower levels of cMV released by endothelial cells and platelets, indicating lower endothelial cell inflammation and platelet activation. These findings contribute to understanding the link between unsuccessful aging, cognitive decline, and vascular cell inflammatory disturbances.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Gemma Chiva-Blanch, Alba Vilella-Figuerola, Teresa Padro, Francesc Formiga, Assumpta Ferrer, Lina Badimon
Summary: The study showed that successful aging is associated with lower levels of cMV released by endothelial cells and platelets, indicating lower endothelial cell inflammation and platelet activation. The results contribute to understanding the link between unsuccessful aging, cognitive decline, and vascular cell inflammatory disturbances.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Chiara Brignole, Veronica Bensa, Nuno A. Fonseca, Genny Del Zotto, Silvia Bruno, Ana F. Cruz, Fabiana Malaguti, Barbara Carlini, Fabio Morandi, Enzo Calarco, Patrizia Perri, Vera Moura, Laura Emionite, Michele Cilli, Francesco De Leonardis, Annalisa Tondo, Loredana Amoroso, Massimo Conte, Alberto Garaventa, Angela R. Sementa, Maria V. Corrias, Mirco Ponzoni, Joao N. Moreira, Fabio Pastorino
Summary: This study showed that cell surface NCL is expressed in NB cell lines, NB tumor cells, and BM-infiltrating NB cells. The targeted nanoparticles decorated with the F3 peptide were effective in inhibiting cell proliferation and reducing tumor growth in NB animal models. NCL may be a promising therapeutic target for NB.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Plant Sciences
Eva Koebke, Lisa Stephan, Markus G. Stetter, Martin Huelskamp
Summary: In this study, the conserved functions of BDCPs were unraveled using the liverwort Marchantia polymorpha. The results showed that MpSPI is involved in cell morphogenesis and salt resistance regulation, and the transcriptomics data confirmed the orthologous gene regulation between MpSPI and AtSPI in Arabidopsis thaliana.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Alisa Mikhaylina, Natalia Lekontseva, Victor Marchenkov, Viktoria Kolesnikova, Albina Khairetdinova, Oleg Nikonov, Vitalii Balobanov
Summary: This study investigated the possibilities and limitations of using circular homo-oligomeric Sm-like proteins as a base for attaching other domains. A circular homoheptameric Sm-like protein from Sulfolobus acidocaldarius was chosen as a stable backbone, and the apical domain of the GroEL chaperone protein was attached. The engineered fusion protein exhibited similar binding and molecular chaperone activity as the full-length GroEL chaperone, demonstrating the effectiveness of this protein engineering approach for creating and stabilizing oligomeric proteins.
Article
Food Science & Technology
Xiaohu Zhou, Chaohua Zhang, Wenhong Cao, Chunxia Zhou, Huina Zheng, Liangzhong Zhao
Summary: The study reports the more effective method of co-precipitation for producing protein mixtures from pea and grass carp. The results showed that the protein co-precipitates had higher subunit ratios, solubility, and foaming activity compared to isolated protein blends, indicating better functional properties. Co-precipitation was found to be more effective than blending for preparing mixed protein using pea and grass carp.
Article
Genetics & Heredity
Xin He, Linai Kuang, Zhiping Chen, Yihong Tan, Lei Wang
Summary: A novel prediction method called KFPM is proposed in this paper, which effectively integrates multiple biological features to infer potential essential proteins based on an improved PageRank algorithm. Experimental results show that KFPM significantly outperforms 13 state-of-the-art prediction methods in predicting candidate proteins, indicating its potential as a meaningful tool for future prediction of potential essential proteins.
FRONTIERS IN GENETICS
(2021)
Article
Biotechnology & Applied Microbiology
Antonela Fuselli, Maria de Los Milagros Burgi, Ricardo Kratje, Claudio Prieto
Summary: In this study, the soluble domain of hSCF was expressed in HEK293 cells, resulting in highly purified rhSCF with increased proliferative activity compared to bacteria-derived hSCF. Three monoclonal antibodies (mAbs) with high specificity for the glycoprotein were obtained using hybridoma technology. These antibodies were able to detect bacteria-derived and glycosylated rhSCF, and were suitable for quantitative analysis and immunodetection.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiaojiao Zhang, Xinman Wang, Xiaonan Dong, Fei Wang, Lingling Cao, Shuangtao Li, Ziji Liu, Xichun Zhang, Yang-Dong Guo, Bing Zhao, Na Zhang
Summary: In this study, the characteristics of the SlTLP family were investigated, revealing their important role in plant growth and development. The SlTLP genes were classified into two subgroups, with the members containing the F-box domain renamed as SlTLFPs. Most of the SlTLP proteins were localized in the nucleus, and their expression was found to be influenced by biotic and abiotic stress. Furthermore, the members with an F-box domain were shown to interact with specific proteins to form complexes and function as E3 ligases through the F-box domain.
Article
Multidisciplinary Sciences
Xun Chen, Matteo Gentili, Nir Hacohen, Aviv Regev
Summary: CeVICA is a cell-free nanobody engineering platform using ribosome display and computational clustering analysis for in vitro selection. It has successfully developed nanobodies against the RBD of SARS-CoV-2 spike protein, with 30 identified as true RBD binders and 11 able to inhibit SARS-CoV-2 pseudotyped virus infection.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yingli Sui, Kun Lu, Lin Fu
Summary: This study identified novel potential key genes ITGAX, LAPTM5, and SERPINE1 for CCRCC through gene expression profiles and survival analysis, indicating their relevance to the occurrence, development, and prognosis of CCRCC.
Article
Immunology
Te-Sheng Lien, Hao Chan, Der-Shan Sun, Jhen-Cheng Wu, You-Yen Lin, Guan-Ling Lin, Hsin-Hou Chang
Summary: The study suggests that EIII could be considered as a virulence factor of DENV, and that Nlrp3 inflammasome is a feasible target for developing therapeutic approaches against dengue-induced platelet defects. Blockages of EIII-induced platelet signaling using inhibitors markedly ameliorated DENV- and EIII-induced thrombocytopenia, platelet activation, and cell death.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Dermatology
Musin Kelel, Ruey-Bing Yang, Tsen-Fang Tsai, Pi-Hui Liang, Fu-Yu Wu, Yu-Tien Huang, Ming-Fong Yang, Yu-Ping Hsiao, Li-Fang Wang, Chen-Fen Tu, Fu-Tong Liu, Yungling L. Lee
Summary: The study reveals that FUT8 remodeling of EGFR plays a critical role in the development of psoriasis phenotypes and is associated with disease severity. FUT8 gain of function promotes cell proliferation, while short hairpin FUT8 reduces proliferation and alters the cell cycle.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Genetics & Heredity
Yuh-Charn Lin, Marcello Niceta, Valentina Muto, Barbara Vona, Alistair T. Pagnamenta, Reza Maroofian, Christian Beetz, Hermine van Duyvenvoorde, Maria Lisa Dentici, Peter Lauffer, Sadeq Vallian, Andrea Ciolfi, Simone Pizzi, Peter Bauer, Nana-Maria Gruening, Emanuele Bellacchio, Andrea Del Fattore, Stefania Petrini, Ranad Shaheen, Dov Tiosano, Rana Halloun, Ben Pode-Shakked, Hatice Mutlu Albayrak, Emreguel Isik, Jan M. Wit, Marcus Dittrich, Bruna L. Freire, Debora R. Bertola, Alexander A. L. Jorge, Ortal Barel, Ataf H. Sabir, Amal M. J. Al Tenaiji, Sulaima M. Taji, Nouriya Al-Sannaa, Hind Al-Abdulwahed, Maria Cristina Digilio, Melita Irving, Yair Anikster, Gandham S. L. Bhavani, Katta M. Girisha, Thomas Haaf, Jenny C. Taylor, Bruno Dallapiccola, Fowzan S. Alkuraya, Ruey-Bing Yang, Marco Tartaglia
Summary: Bi-allelic inactivating variants in SCUBE3 gene result in a previously unrecognized syndromic disorder characterized by growth retardation, skeletal abnormalities, distinctive craniofacial features, and dental anomalies. Functional studies demonstrate the involvement of SCUBE3 in modulating BMP signaling pathway to regulate growth, morphogenesis, bone, and teeth development. Knockout mice for SCUBE3 exhibit similar phenotypes, implicating the crucial role of SCUBE3 in these processes.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Biology
Ya-Lang Huang, Ming-Ting Huang, Pei-Shan Sung, Teh-Ying Chou, Ruey-Bing Yang, An-Suei Yang, Chung-Ming Yu, Yu-Wen Hsu, Wei-Chiao Chang, Shie-Liang Hsieh
Summary: Ya-Lang Huang et al. report a mechanism for TLR3-mediated signaling after immune simulation and influenza virus infection by way of the co-receptor CLEC18A. This study found that a single amino acid change in CLEC18A(S339R) can enhance the production of type I and type III interferons to suppress viral replication, and increase mice survival rate after flu infection infection.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Ku-Chi Tsao, Yuh-Charn Lin, Yi-Ting Chen, Shih-Lei Lai, Ruey-Bing Yang
Summary: This study demonstrates the critical cooperation between SCUBE1 and SCUBE2 in regulating angiogenic cell behaviors and the formation of functional vessels during zebrafish embryonic development.
CARDIOVASCULAR RESEARCH
(2022)
Article
Engineering, Biomedical
Yu-Feng Hu, An-Sheng Lee, Shih-Lin Chang, Shien-Fong Lin, Ching-Hui Weng, Hsin-Yu Lo, Pei-Chun Chou, Yung-Nan Tsai, Yen-Ling Sung, Chien-Chang Chen, Ruey-Bing Yang, Yuh-Charn Lin, Terry B. J. Kuo, Cheng-Han Wu, Jin-Dian Liu, Tze-Wen Chung, Shih-Ann Chen
Summary: A silk fibroin hydrogel can directly convert rat quiescent ventricular cardiomyocytes into pacemaker cardiomyocytes by inducing the ectopic expression of the vascular endothelial cell-adhesion glycoprotein cadherin, exhibiting functional and morphological features of genuine sinoatrial-node cardiomyocytes both in vitro and in vivo. This approach could provide a scalable production of biological pacemakers for human use by utilizing biomaterials with suitable properties.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Cardiac & Cardiovascular Systems
Pei-Chun Chou, Chih-Min Liu, Ching-Hui Weng, Kai-Chien Yang, Mei-Ling Cheng, Yuh-Charn Lin, Ruey-Bing Yang, Bai-Chuang Shyu, Song-Kun Shyue, Jin-Dian Liu, Shih-Pin Chen, Michael Hsiao, Yu-Feng Hu
Summary: This study reveals how fibroblasts drive the normal function of the sinoatrial node (SAN) by regulating metabolic reprogramming and rhythmic activity. The study also identifies the critical role of Aldoc in the interaction between fibroblasts and pacemaker cardiomyocytes.
CIRCULATION RESEARCH
(2022)
Article
Hematology
Hem Kumar Tamang, Ruey-Bing Yang, Zong-Han Song, Shao-Chun Hsu, Chien-Chung Peng, Yi-Chung Tung, Bing-Hsiean Tzeng, Chien-Chang Chen
Summary: This study explores the expression and role of Ca(v)3.2 in platelet function, revealing its importance for optimal platelet activation.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Biochemistry & Molecular Biology
Shuo-Ming Ou, Ming-Tsun Tsai, Huan-Yuan Chen, Fu-An Li, Kuo-Hua Lee, Wei-Cheng Tseng, Fu-Pang Chang, Yao-Ping Lin, Ruey-Bing Yang, Der-Cherng Tarng
Summary: Plasma galectin-3 is associated with organ fibrosis, and this study explored the potential of urinary galectin-3 as a biomarker for kidney disease progression. Patients with higher urinary galectin-3 levels had lower eGFRs and higher proteinuria levels. Higher levels of urinary galectin-3 were also associated with more severe renal fibrosis. The study suggests that urinary galectin-3 levels may be useful for identifying patients at high risk of kidney disease progression and renal fibrosis.
Article
Cell Biology
Yingzi Liu, Christian F. Guerrero-Juarez, Fei Xiao, Nitish Udupi Shettigar, Raul Ramos, Chen-Hsiang Kuan, Yuh-Charn Lin, Luis de Jesus Martinez Lomeli, Jung Min Park, Ji Won Oh, Ruiqi Liu, Sung-Jan Lin, Marco Tartaglia, Ruey-Bing Yang, Zhengquan Yu, Qing Nie, Ji Li, Maksim Plikus
Summary: Hair follicle stem cells are regulated by dermal papilla fibroblasts, which promote hair growth by secreting factors and influencing the mesenchymal niche through the Scube3/TGF-beta mechanism.
DEVELOPMENTAL CELL
(2022)
Article
Pharmacology & Pharmacy
Yue-Zhi Lee, Hsing-Yu Hsu, Cheng-Wei Yang, Yi-Ling Lin, Sui-Yuan Chang, Ruey-Bing Yang, Jian-Jong Liang, Tai-Ling Chao, Chun-Che Liao, Han-Chieh Kao, Jang-Yang Chang, Huey-Kang Sytwu, Chiung-Tong Chen, Shiow-Ju Lee
Summary: Ciclesonide blocks coronavirus-induced cytokine production by increasing I kappa B alpha protein levels and decreasing p65 nuclear translocation, and it can be used in the treatment of COVID-19 patients. The combination of Ciclesonide and dbq33b synergistically decreases cytokine levels and inhibits coronavirus replication. Therefore, this combination therapy is a promising treatment option for COVID-19 patients with high viral loads and NF-kappa B-mediated cytokine storms.
Article
Hematology
Binay K. Sahoo, Yuh-Charn Lin, Cheng-Fen Tu, Wei-Ju Liao, Ling -Hui Li, Kurt Yun Mou, Shu-Ping Wang, Chi-Tai Yeh, Chi-Yuan Yao, Hsin-An Hou, Wen -Chien Chou, Hwei-Fang Tien, Ruey-Bing Yang
Summary: A hallmark of MLL-r acute myeloid leukemia is addiction to protein tyrosine kinase signaling, particularly FLT3 signaling. This study reveals that SCUBE1 is a direct target of HOXA9/MEIS1 and is highly expressed on the MLL-r cell surface. SCUBE1 binds to both the FLT3 ligand and the extracellular ligand-binding domains of FLT3, facilitating activation of the FLT3-LYN signaling axis. Targeting surface SCUBE1 with an antibody-drug conjugate leads to decreased cell viability specifically in MLL-r leukemia. SCUBE1 is a potential therapeutic target for MLL rearrangement-induced leukemia.
Correction
Pharmacology & Pharmacy
Yue-Zhi Lee, Hsing-Yu Hsu, Cheng-Wei Yang, Yi-Ling Lin, Sui-Yuan Chang, Ruey-Bing Yang, Jian-Jong Liang, Tai-Ling Chao, Chun-Che Liao, Han-Chieh Kao, Jang-Yang Chang, Huey-Kang Sytwu, Chiung-Tong Chen, Shiow-Ju Lee
Review
Cell Biology
Yuh-Charn Lin, Binay K. Sahoo, Shiang-Shin Gau, Ruey-Bing Yang
Summary: The SCUBE family consists of three highly conserved proteins in vertebrates that play important roles in various physiological and pathological processes. Both soluble and membrane-associated SCUBEs are involved in signaling pathways and have been implicated in cancer, skeletal disease, and cardiovascular disease.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Oncology
Cheng-Fen Tu, Fu-An Li, Ling-Hui Li, Ruey-Bing Yang
Summary: Core fucosylation mediated by FUT8 enhances signaling of TGF-beta receptor, promoting breast cancer invasion and metastasis. Quantitative glycoproteomics identified 140 common core-fucosylated target glycoproteins, while pathway analysis highlighted crucial signaling networks like core fucosylation of integrin alpha v beta 5 or IL6ST for breast cancer cell invasion and migration.
BREAST CANCER RESEARCH
(2022)